Endothelium : journal of endothelial cell research最新文献

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31P magnetic resonance spectroscopy of endothelial cells grown in three-dimensional matrigel constructs as an enabling platform technology: II. The effect of anti-inflammatory drugs on phosphometabolite levels. 在三维基质结构中生长的内皮细胞的31P磁共振波谱作为支持平台技术:消炎药对磷代谢水平的影响。
Endothelium : journal of endothelial cell research Pub Date : 2008-09-01 DOI: 10.1080/10623320802487874
I Ringel, S Lecht, M Sterin, P I Lelkes, P Lazarovici
{"title":"31P magnetic resonance spectroscopy of endothelial cells grown in three-dimensional matrigel constructs as an enabling platform technology: II. The effect of anti-inflammatory drugs on phosphometabolite levels.","authors":"I Ringel,&nbsp;S Lecht,&nbsp;M Sterin,&nbsp;P I Lelkes,&nbsp;P Lazarovici","doi":"10.1080/10623320802487874","DOIUrl":"https://doi.org/10.1080/10623320802487874","url":null,"abstract":"<p><p>In the accompanying study, the authors presented phosphometabolite patterns of endothelial cells grown under three-dimensional (3D) conditions using (31)P magnetic resonance spectroscopy (MRS). Here the authors describe the effect of nonsteroidal anti-inflammatory drugs (NSAIDs), using this enabling platform technology, which is relevant for evaluating drug effects in tissue-engineered endothelial constructs. Treatment with indomethacin significantly changed the phosphometabolite fingerprint in this endothelial model, by, respectively, increasing (81%) and decreasing (42%) glycerophosphocholine (GPC) and phosphomonoesters (PM). Furthermore, a safer approach using a NSAID prodrug was also demonstrated in this study with a indomethacin phospholipid-derived prodrug (DP-155). Like the parental drug, DP-155 increased and decreased the levels of GPC and PM by 100% and 20%, respectively. These changes represent useful biomarkers to monitor NSAID effects on endothelized tissue-engineered constructs for the purpose of controlling endothelial cell survival and inflammation upon implantation.</p>","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320802487874","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27885745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Endothelial progenitor cells in patients with severe peripheral arterial disease. 内皮祖细胞在严重外周动脉疾病患者中的应用
Endothelium : journal of endothelial cell research Pub Date : 2008-09-01 DOI: 10.1080/10623320802487718
Pietro Delva, Sergio De Marchi, Manlio Prior, Maurizio Degan, Alessandro Lechi, Maristella Trettene, Enrico Arosio
{"title":"Endothelial progenitor cells in patients with severe peripheral arterial disease.","authors":"Pietro Delva,&nbsp;Sergio De Marchi,&nbsp;Manlio Prior,&nbsp;Maurizio Degan,&nbsp;Alessandro Lechi,&nbsp;Maristella Trettene,&nbsp;Enrico Arosio","doi":"10.1080/10623320802487718","DOIUrl":"https://doi.org/10.1080/10623320802487718","url":null,"abstract":"<p><p>The aims of this study were to investigate the interrelationships between endothelial progenitor cells (EPCs), peripheral arterial disease (PAD), and atherosclerotic risk factors, as only limited data are available regarding the EPCs in patients with PAD. The authors studied the number of EPCs by different methods in a carefully selected group of 45 patients with PAD along with 24 healthy subjects (HS). In patients with PAD, by utilizing the dual-binding method, the number of EPCs was significantly increased compared to HS (M +/- SD, PAD: 73 +/- 33, HS: 52 +/- 20 EPCs/high power field; p < .001). On the contrary, both CD34(+) cell count and CD133(+) cell count were significantly decreased compared to HS. Colony-forming units were significantly increased in PAD compared to HS (median and 25th and 75th percentiles, PAD: 7, 1, 9; HS: 1, 1, 4 CFU/well, respectively; Mann-Whitney, p = .006). In patients with PAD, the number and proliferative activity of circulating EPCs are increased with respect to HS even though EPC count by flourecence-activated cell sorting (FACS) analysis provided different results and this may explain the discrepancy in data collected using different methods. The regulation of the number and biological activity of EPCs in PAD remains unclear.</p>","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320802487718","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27885761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 19
31P magnetic resonance spectroscopy of endothelial cells grown in three-dimensional matrigel construct as an enabling platform technology: I. The effect of glial cells and valproic acid on phosphometabolite levels. 三维基质构建中内皮细胞的31P磁共振波谱作为支持平台技术:1 .胶质细胞和丙戊酸对磷代谢产物水平的影响。
Endothelium : journal of endothelial cell research Pub Date : 2008-09-01 DOI: 10.1080/10623320802487841
M Sterin, I Ringel, S Lecht, P I Lelkes, P Lazarovici
{"title":"31P magnetic resonance spectroscopy of endothelial cells grown in three-dimensional matrigel construct as an enabling platform technology: I. The effect of glial cells and valproic acid on phosphometabolite levels.","authors":"M Sterin,&nbsp;I Ringel,&nbsp;S Lecht,&nbsp;P I Lelkes,&nbsp;P Lazarovici","doi":"10.1080/10623320802487841","DOIUrl":"https://doi.org/10.1080/10623320802487841","url":null,"abstract":"<p><p>Very few studies describe endothelial cell (EC) properties under three-dimensional (3D) conditions using (31)P magnetic resonance spectroscopy (MRS). The authors developed a model in which living ECs growing in Matrigel threads (3D conditions) for 5 days are monitored by (31)P MRS, providing the fingerprint of the major EC phosphometabolites. Organic extracts of membranal phospholipids were also analyzed by (31)P MRS. For comparison and as a model for two-dimensional (2D) tissue culture conditions, (31)P MRS spectra of aqueous extracts of EC phosphometabolites grown under 2D conditions were also evaluated. The phosphometabolites fingerprint of the cells cultured under 3D was significantly different from that of ECs maintained under 2D. Moreover, the pattern of phosphometabolites was affected by coculture with C6-glioma cells and upon treatment with valproic acid, which is under clinical investigation as an antioangiogenic anticancer drug. The major effects were modulation of (i) energy metabolism intermediates such as phosphocreatine, (ii) precursors of phospholipids such as phosphomonoesters, and (iii) degradation products of phospholipids such as glycerophosphocholine. This endothelial model will be usefull as an enabling platform technology for tissue engineering.</p>","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320802487841","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27885744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Physiological pathway for low-density lipoproteins across the blood-brain barrier: transcytosis through brain capillary endothelial cells in vitro. 低密度脂蛋白穿过血脑屏障的生理途径:体外通过脑毛细血管内皮细胞的转囊作用。
Endothelium : journal of endothelial cell research Pub Date : 2008-09-01 DOI: 10.1080/10623320802487759
Pietra Candela, Fabien Gosselet, Florence Miller, Valerie Buee-Scherrer, Gérard Torpier, Roméo Cecchelli, Laurence Fenart
{"title":"Physiological pathway for low-density lipoproteins across the blood-brain barrier: transcytosis through brain capillary endothelial cells in vitro.","authors":"Pietra Candela, Fabien Gosselet, Florence Miller, Valerie Buee-Scherrer, Gérard Torpier, Roméo Cecchelli, Laurence Fenart","doi":"10.1080/10623320802487759","DOIUrl":"10.1080/10623320802487759","url":null,"abstract":"<p><p>Although an immense knowledge has accumulated concerning regulation of cholesterol homeostasis in the body, this does not include the brain, where details are just emerging. Using an in vitro blood-brain barrier model, the authors have demonstrated that low-density lipoprotein (LDL) underwent transcytosis through the endothelial cells (ECs) by a receptor-mediated process, bypassing the lysosomal compartment. Moreover, caveolae might be involved in these blood-borne molecule transports from the blood to the brain. Although several ligands are known to be internalized through cell surface caveolae, the subsequent intracellular pathways have remained elusive. By cell fractionation experiment and Western blot, the authors have demonstrated that the LDL receptor is located in the caveolae membrane fraction. Then, LDLs internalized were detected by electron microscopy in multivesicular bodies. The authors identified in brain capillary ECs a novel endosomal compartment, mildly acidic, positive for marker Lamp-1 but devoid of any degradative capability. From the point of view of pH, cellular location, and caveolae-derived formation, the multivesicular organelles described here can be related to the caveosome structure. These results could provide clues to physiological functions of caveolae-caveosome transcellular pathway in brain capillary ECs and may help in the rational design of more effective therapeutic drugs to the brain.</p>","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320802487759","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27885764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 97
Interaction of estrogen and tumor necrosis factor alpha in endothelial cell migration and early stage of angiogenesis. 雌激素和肿瘤坏死因子α在内皮细胞迁移和血管生成早期的相互作用。
Endothelium : journal of endothelial cell research Pub Date : 2008-09-01 DOI: 10.1080/10623320802487775
Maria Florian, Livia Florianova, Sabah Hussain, Sheldon Magder
{"title":"Interaction of estrogen and tumor necrosis factor alpha in endothelial cell migration and early stage of angiogenesis.","authors":"Maria Florian,&nbsp;Livia Florianova,&nbsp;Sabah Hussain,&nbsp;Sheldon Magder","doi":"10.1080/10623320802487775","DOIUrl":"https://doi.org/10.1080/10623320802487775","url":null,"abstract":"<p><p>The role of estrogen replacement therapy in postmenopausal women remains controversial. The authors hypothesized that contradictory results with estrogen therapy may be explained by estrogen's potent proangiogenic property, which could be protective in women without atherosclerotic disease but in the presence of chronic inflammation, could lead to destabilization of atherosclerotic plaques. The authors thus examined the interaction between 17beta-estradiol (E2) and the inflammatory cytokine tumor necrosis factor alpha (TNFalpha) in an early stage of angiogenesis. Human umbilical endothelial cells were grown to confluence. Migration was assessed with a wound assay and proliferation was assessed with 5-bromo-2'-deoxyuridine (BrDU). Cells were treated with medium alone, TNFalpha at 0.3, 1, or 20 ng/ml, E2 at 20 nM, or the combination of E2 and TNFalpha. The authors used real-time polymerase chain reaction (PCR) to measure changes in expression of the angiogenesis genes angiopoeitin-2 (Ang-2), vacular endothelial growth factor (VEGF)-A and -C, and interleukin (IL)-8. A large dose of TNFalpha (20 ng/ml) inhibited healing at 24 to 48 h and the addition of E2 preserved some healing. E2 by itself doubled migration, with only a minimal effect on proliferation. A low dose of TNFalpha (0.3 ng/ml) had no effect on migration, 1.0 ng/ml moderately increased it, but the addition of E2 to both doses of TNFalpha increased migration. There was no change in migration when cells were pretreated with E2 and given TNFalpha after wounding, whereas pretreatment with TNFalpha followed by E2 significantly increased wound healing. The nitric oxide synthase (NOS) inhibitor N-nitro-l-arginine-methyl ester (l-NAME) completely blocked the E2 effect on migration. TNFalpha (0.3 and 1.0 ng/ml) increased expression of VEGF-C (2.8 +/- 0.1- and 2.5 +/- 0.2-fold, respectively) and IL-8 (32.8 +/- 1.2- and 42.7 +/- 3.6-fold, respectively) mRNA, but E2 had no significant effect on these molecules. E2 increases the angiogenic activity of TNFalpha. This could potentially worsen the stability of complex atherosclerotic plaques and increase cardiovascular events.</p>","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320802487775","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27885747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Effect of nicotine and nicotine metabolites on angiotensin-converting enzyme in human endothelial cells. 尼古丁及其代谢物对人内皮细胞血管紧张素转换酶的影响。
Endothelium : journal of endothelial cell research Pub Date : 2008-09-01 DOI: 10.1080/10623320802487627
Liza U Ljungberg, Karin Persson
{"title":"Effect of nicotine and nicotine metabolites on angiotensin-converting enzyme in human endothelial cells.","authors":"Liza U Ljungberg,&nbsp;Karin Persson","doi":"10.1080/10623320802487627","DOIUrl":"https://doi.org/10.1080/10623320802487627","url":null,"abstract":"<p><p>Nicotine has been shown to induce endothelial dysfunction, which is an early marker of atherosclerosis. Nicotine undergoes extensive metabolism in the liver, forming a number of major and minor metabolites. There are very limited data on the effect of nicotine metabolites on the cardiovascular system. This study investigates the effects of nicotine and the nicotine metabolites, cotinine, cotinine-N-oxide, nicotine-1'-N-oxide, norcotinine, trans-3'-hydroxycotinine, on angiotensin-converting enzyme (ACE) in human endothelial cells. Cultured endothelial cells obtained from human umbilical cord vein (HUVECs) were stimulated with nicotine or nicotine metabolites in concentrations similar to those observed in plasma during smoking. ACE activity and expression were analyzed using commercial kits. The results showed that nicotine and nicotine metabolites can increase both activity and expression of ACE. However, a marked individual variation in the response to the drugs was observed. This variation was not associated with the ACE insertion/deletion polymorphism. Tobacco contains numerous chemical compounds, and the underlying cause for development of atherosclerosis in smokers is probably multifactorial. The results from this study could explain one cellular mechanism by which smoking exerts negative effect on the vascular system.</p>","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320802487627","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27886455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
Effects of two complex hemodynamic stimulation profiles on hemostatic genes in a vessel-like environment. 血管样环境中两种复杂血流动力学刺激对止血基因的影响。
Endothelium : journal of endothelial cell research Pub Date : 2008-09-01 DOI: 10.1080/10623320802487536
N Bergh, E Ulfhammer, L Karlsson, S Jern
{"title":"Effects of two complex hemodynamic stimulation profiles on hemostatic genes in a vessel-like environment.","authors":"N Bergh,&nbsp;E Ulfhammer,&nbsp;L Karlsson,&nbsp;S Jern","doi":"10.1080/10623320802487536","DOIUrl":"https://doi.org/10.1080/10623320802487536","url":null,"abstract":"<p><p>Endothelial cells are the main sensors of changes in the biomechanical flow environment and play a pivotal role in vascular homeostasis. An in vitro perfusion model was developed to study the regulatory effect on gene expression by different flow and pressure profiles. Human umbilical vein endothelial cells were grown to confluence inside capillary microslides or silicone tubes. Thereafter, they were exposed to different levels of shear stress or different levels of static or pulsatile pressure. Genes representing various hemostasis functions of the endothelial cells were analyzed. Shear stress was a more effortful stimulus than static or pulsatile tensile stress. Although shear stress affected mRNA expression of all six studied genes (tissue-type plasminogen activator [t-PA], plasminogen activator inhibitor [PAI]-1, Thrombomodulin [TM], urokinase-type plasminogen activator [u-PA], vascular cell adhesion molecule [VCAM-1], and endothelial nitric oxide synthase [eNOS]), none of the genes was found regulated by pressure. Shear stress down-regulated t-PA and VCAM-1 in a dose response-dependent way, and up-regulated TM. u-PA, eNOS, and PAI-1 were up-regulated by shear stress, but there was no obvious dose-response effect for these genes. These findings suggest that shear stress has a more powerful gene regulatory effect on endothelial gene expression than tensile stress. Low shear stress induced a more proatherogenic endothelial surface but preserved t-PA gene expression levels compared to high shear stress.</p>","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320802487536","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27885762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
p66Shc deletion confers vascular protection in advanced atherosclerosis in hypercholesterolemic apolipoprotein E knockout mice. p66Shc缺失在高胆固醇血症载脂蛋白E敲除小鼠晚期动脉粥样硬化中赋予血管保护作用。
Endothelium : journal of endothelial cell research Pub Date : 2008-09-01 DOI: 10.1080/10623320802487791
Ines Martin-Padura, Filomena de Nigris, Enrica Migliaccio, Gelsomina Mansueto, Simone Minardi, Monica Rienzo, Lilach O Lerman, Massimo Stendardo, Marco Giorgio, Gaetano De Rosa, Pier Giuseppe Pelicci, Claudio Napoli
{"title":"p66Shc deletion confers vascular protection in advanced atherosclerosis in hypercholesterolemic apolipoprotein E knockout mice.","authors":"Ines Martin-Padura,&nbsp;Filomena de Nigris,&nbsp;Enrica Migliaccio,&nbsp;Gelsomina Mansueto,&nbsp;Simone Minardi,&nbsp;Monica Rienzo,&nbsp;Lilach O Lerman,&nbsp;Massimo Stendardo,&nbsp;Marco Giorgio,&nbsp;Gaetano De Rosa,&nbsp;Pier Giuseppe Pelicci,&nbsp;Claudio Napoli","doi":"10.1080/10623320802487791","DOIUrl":"https://doi.org/10.1080/10623320802487791","url":null,"abstract":"<p><p>Previous studies showed that p66(Shc-/-) mice on a very-high-fat diet (HFD) had reduced oxidative stress, foam cell, and early atherosclerotic lesion formation. Here, the authors have used hypercholesterolemic apolipoprotein E (ApoE(-/-)) mice to investigate the role of p66Shc deletion in advanced atheroma. The authors generated mice deficient of both ApoE and p66Shc genes (ApoE(-/-) /p66(Shc-/-)). They used microsatellite polymerase chain reaction (PCR) analysis to analyze the genetic background and considered only animals with a constant percentages of C57B6L and 129SV background strands (it was obtained the 50.3% +/- 6.4% of C57B6L background). Computer-assisted analysis revealed that advanced atherosclerotic lesions in ApoE(-/-)/p66(Shc+/+) were significantly larger than those observed in ApoE(-/-)/p66(Shc-/-). Accordingly, the lipid-laden macrophage foam cells and oxidation-specific epitopes in ApoE(-/-)/p66(shc+/+) HFD-treated groups were higher than those observed in normal diet (ND)-treated groups. Thus, p66(Shc-/-) plays an important protective role also against advanced atherosclerotic lesion formation. Finally, the authors have used microarray to investigate major changes in gene expression in aortas of mice with ApoE(-/-)/p66(Shc-/-) background treated with a very HFD in comparison to ApoE(-/-)/p66(Shc+/+) (these data have been confirmed by by real-time PCR and immunohistochemistry). DAVID (Database for Annotation, Visualization and Integrated Discovery) analysis revealed that CD36 antigen (CD36), tissue inhibitor of metalloproteinase 2 (TIMP2), apolipoprotein E (ApoE), acetyl-coenzyme A acetyltransferase 1 (ACAT1), and thrombospondin 1 (THBS1) can be involved in p66 deletion-dependent vascular protection through the adipocytokine/lipid signaling pathway.</p>","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320802487791","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27885767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 50
Protein kinase C-mediated phosphorylation of p47 phox modulates platelet-derived growth factor-induced H2O2 generation and cell proliferation in human umbilical vein endothelial cells. 蛋白激酶c介导的p47 phox磷酸化调节血小板衍生生长因子诱导的H2O2生成和人脐静脉内皮细胞增殖。
Endothelium : journal of endothelial cell research Pub Date : 2008-07-01 DOI: 10.1080/10623320802174480
F Simon, A Stutzin
{"title":"Protein kinase C-mediated phosphorylation of p47 phox modulates platelet-derived growth factor-induced H2O2 generation and cell proliferation in human umbilical vein endothelial cells.","authors":"F Simon,&nbsp;A Stutzin","doi":"10.1080/10623320802174480","DOIUrl":"https://doi.org/10.1080/10623320802174480","url":null,"abstract":"<p><p>Substantial evidence indicate that growth factors such as platelet-derived growth factor (PDGF) exert their effect, at least in part, through reactive oxygen species (ROS) generated via NAD(P)H oxidase. In this work, the role of p47(phox), a key component of the phagocytic NAD(P)H oxidase in cell proliferation, was addressed. The authors show that diphenylene iodonium (DPI) and apocynin, but not N(G)-nitro-L-arginine methyl esterL-NAME, reduced PDGF-induced ROS generation and proliferation in human umbilical vein endothelial cells (HUVECs). Pharmacological inhibition of protein kinase C (PKC) as well as dominant-negative mutants of p47(phox) directed to PKC-dependent phosphorylation targets inhibited PDGF-stimulated ROS production and cell proliferation. Hydrogen peroxide restored PDGF-stimulated proliferation in cells that was inhibited by apocynin, DPI, or by the dominant-negative mutants. PDGF-induced proliferation was reduced in the HUVEC-derived cell line E.A.hy926 overexpressing catalase. On the contrary, cells overexpressing superoxide dismutase 1 exhibited increased proliferation. These results demonstrate that PKC-dependent phosphorylation of p47(phox) is essential for PDGF-stimulated ROS generation and proliferation in HUVECs. More relevant, H(2)O(2) is identified as the key molecule that signals proliferation in the systems studied.</p>","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320802174480","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27564796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 34
Polymorphisms of the endothelin-1 gene associate with hypertension in patients with rheumatoid arthritis. 内皮素-1基因多态性与类风湿关节炎患者高血压相关
Endothelium : journal of endothelial cell research Pub Date : 2008-07-01 DOI: 10.1080/10623320802228708
Vasileios F Panoulas, Karen M J Douglas, Jacqueline P Smith, Patrick Taffé, Antonios Stavropoulos-Kalinoglou, Tracey E Toms, Moses S Elisaf, Peter Nightingale, George D Kitas
{"title":"Polymorphisms of the endothelin-1 gene associate with hypertension in patients with rheumatoid arthritis.","authors":"Vasileios F Panoulas,&nbsp;Karen M J Douglas,&nbsp;Jacqueline P Smith,&nbsp;Patrick Taffé,&nbsp;Antonios Stavropoulos-Kalinoglou,&nbsp;Tracey E Toms,&nbsp;Moses S Elisaf,&nbsp;Peter Nightingale,&nbsp;George D Kitas","doi":"10.1080/10623320802228708","DOIUrl":"https://doi.org/10.1080/10623320802228708","url":null,"abstract":"<p><p>Rheumatoid arthritis (RA) associates with excess cardiovascular (CV) morbidity and mortality. Hypertension, a highly prevalent entity in RA, has been associated with the endothelin-1 (ET-1) gene locus (EDN1) in some groups, such as Afro-Caribbean, the obese, and in low-renin states, but not in the general population as a whole. High levels of plasma ET-1 have been observed in RA. This study evaluated the potential association of EDN1 gene locus and serum ET-1 levels with hypertension in patients with RA. Genomic DNA and serum samples were collected from 397 well-characterized RA patients; DNA was also available from 401 local general population controls without RA. To explore the overall relevance of EDN1, two suitable single-nucleotide polymorphisms (SNPs), rs1800541 and rs5370, were selected and haplotype analysis was performed. Both SNPs were identified using real-time polymerase chain reaction (PCR) and melting curve analysis. Genetic analysis was related to hypertension as dichotomous trait and to blood pressure indices as continuous variables. Serum endothelin levels were also assessed in the RA patients. No genotype or haplotype differences were observed between RA and control subjects. Within RA, logistic regression analysis of each SNP separately revealed a threefold increase in the adjusted odds of being hypertensive of rs5370 TT homozygotes compared to GG homozygotes (OR = 2.89, 95%CI: 1.02 to 8.19). After adjustment for multiple potential confounders, haplotype analysis revealed an additive effect of the rs1800541-rs5370 T-T haplotype on hypertension (OR = 2.96, 95%CI: 1.28 to 6.86; p = .011), systolic blood pressure (SBP) (beta = 6.75 +/- 2.57 mm Hg; p = .009), and pulse pressure (PP) (beta = 4.37 +/- 2.12 mm Hg; p = .040). There was an increased prevalence of raised ET-1 levels amongst hypertensive RA patients, whereas a similar trend was observed for T-T haplotype carriers. RA patients who carry the rs1800541-rs5370 T-T EDN1 haplotype appear more likely to be hypertensive with an increased SBP and PP. These findings, if replicated in future studies, could be used as a screening tool for RA patients at increased hypertension, and thus cardiovascular, risk.</p>","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320802228708","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27564798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 55
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