31P magnetic resonance spectroscopy of endothelial cells grown in three-dimensional matrigel constructs as an enabling platform technology: II. The effect of anti-inflammatory drugs on phosphometabolite levels.

I Ringel, S Lecht, M Sterin, P I Lelkes, P Lazarovici
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引用次数: 1

Abstract

In the accompanying study, the authors presented phosphometabolite patterns of endothelial cells grown under three-dimensional (3D) conditions using (31)P magnetic resonance spectroscopy (MRS). Here the authors describe the effect of nonsteroidal anti-inflammatory drugs (NSAIDs), using this enabling platform technology, which is relevant for evaluating drug effects in tissue-engineered endothelial constructs. Treatment with indomethacin significantly changed the phosphometabolite fingerprint in this endothelial model, by, respectively, increasing (81%) and decreasing (42%) glycerophosphocholine (GPC) and phosphomonoesters (PM). Furthermore, a safer approach using a NSAID prodrug was also demonstrated in this study with a indomethacin phospholipid-derived prodrug (DP-155). Like the parental drug, DP-155 increased and decreased the levels of GPC and PM by 100% and 20%, respectively. These changes represent useful biomarkers to monitor NSAID effects on endothelized tissue-engineered constructs for the purpose of controlling endothelial cell survival and inflammation upon implantation.

在三维基质结构中生长的内皮细胞的31P磁共振波谱作为支持平台技术:消炎药对磷代谢水平的影响。
在相应的研究中,作者使用(31)P磁共振波谱(MRS)展示了在三维(3D)条件下生长的内皮细胞的磷代谢模式。在这里,作者描述了非甾体抗炎药(NSAIDs)的作用,使用这种使能平台技术,这与评估药物在组织工程内皮结构中的作用有关。吲哚美辛显著改变了内皮模型中磷代谢物指纹图谱,分别增加(81%)和减少(42%)甘油酰胆碱(GPC)和磷酸单酯(PM)。此外,使用吲哚美辛磷脂衍生前药(DP-155)的非甾体抗炎药前药也被证明是一种更安全的方法。与亲本药一样,DP-155使GPC和PM水平分别升高100%和降低20%。这些变化是监测非甾体抗炎药对内皮化组织工程构建物的影响的有用生物标志物,目的是控制内皮细胞的存活和植入后的炎症。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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