Endocrinologia japonica最新文献_第7页

Gradual progress of ACTH deficiency in a child with panhypopituitarism associated with pituitary stalk transection. 与垂体柄横断相关的全垂体功能低下儿童ACTH缺乏的逐渐进展。

Endocrinologia japonica Pub Date : 1992-04-01 DOI: 10.1507/endocrj1954.39.165

Y Hasegawa, T Hasegawa, T Yokoyama, S Kotoh, Y Tsuchiya

引用次数: 7

A patient with a prolactinoma associated with an aldosterone producing adrenal adenoma: differences in dopaminergic regulation of PRL and aldosterone secretion. 与醛固酮产生肾上腺腺瘤相关的泌乳素瘤患者:多巴胺能调节PRL和醛固酮分泌的差异

Endocrinologia japonica Pub Date : 1992-04-01 DOI: 10.1507/endocrj1954.39.169

R Demura, M Naruse, M Isawa, N Onoda, K Naruse, M Yamakado, H Demura

{"title":"A patient with a prolactinoma associated with an aldosterone producing adrenal adenoma: differences in dopaminergic regulation of PRL and aldosterone secretion.","authors":"R Demura, M Naruse, M Isawa, N Onoda, K Naruse, M Yamakado, H Demura","doi":"10.1507/endocrj1954.39.169","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.169","url":null,"abstract":"A patient with a rare combination of prolactinoma and aldosterone producing adrenal adenoma (APA) was reported in relation to studies concerning dopaminergic regulation of PRL and aldosterone secretion. The patient is a 38-year-old female with plasma PRL and aldosterone concentrations (PAC) of 563 ng/ml and 54 ng/dl, respectively. A bolus of 10 mg of metoclopramide significantly increased plasma PRL in 6 normal subjects and in 4 patients with APA, whereas the responses were blunted in 7 patients with prolactinoma and in our patient. The response of aldosterone to metoclopramide was less than that of PRL, but similar in all studied subjects, indicating that the dopaminergic inhibition of aldosterone secretion is less than that of PRL in normal subjects and did not change in patients with APA or prolactinoma. Oral administration of 2.5 mg of bromocriptine suppressed plasma PRL significantly in all the subjects studied, but did not produce any consistent changes in PAC. Discrepancies in the response of PRL and aldosterone to metoclopramide and to bromocriptine suggest a difference in the dopaminergic regulation of PRL and aldosterone secretion in both normal subjects and patients with prolactinoma and APA. It is unlikely that reduced dopaminergic inhibition is the basis for hypersecretion of PRL and aldosterone in our patient.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 2","pages":"169-76"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.169","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12568422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

A kindred of multiple endocrine neoplasia type 2B. 一种2B型多发性内分泌肿瘤。

Endocrinologia japonica Pub Date : 1992-02-01 DOI: 10.1507/endocrj1954.39.25

S Yamamoto, I Morimoto, T Fujihira, K Watanabe, K Zeki, K Yoshimoto, F Zeze, S Eto

{"title":"A kindred of multiple endocrine neoplasia type 2B.","authors":"S Yamamoto, I Morimoto, T Fujihira, K Watanabe, K Zeki, K Yoshimoto, F Zeze, S Eto","doi":"10.1507/endocrj1954.39.25","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.25","url":null,"abstract":"We describe familial cases of multiple endocrine neoplasia (MEN) 2B: A 48-year-old man is the proband. He had pheochromocytoma, medullary thyroid carcinomas (MTCs), parathyroid hyperplasia, mucosal neuromas, eversion of eyelids and Marfanoid appearance, and then underwent adrenalectomy and total thyroidectomy. Family screening revealed that his two daughters (10 and 8 years old) had mucosal neuromas and increased serum calcitonin (CT). Both of them had MTCs but no pheochromocytoma, and their MTCs were surgically removed. The father and his children have been in favorable condition since the operations. Southern blot analysis with 33 polymorphic DNA probes was done in MTCs obtained from two daughters. An RBP3 (10q11.2) locus linked to a predisposing gene on chromosome 10 was uninformative in either patient because of constitutional homozygosity. Loss of heterozygosity at the MYCL1 locus on chromosome 1p32 was observed in MTC from the younger sister, but no loss of heterozygosity was recognized in other loci examined. Deletion of the 1p32 locus may play a role in the development of MEN 2B.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 1","pages":"25-30"},"PeriodicalIF":0.0,"publicationDate":"1992-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.25","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12523909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Radioimmunoassay for calcitonin gene-related peptide and its measurement in sera of patients with thyroid disease. 甲状腺疾病患者血清降钙素基因相关肽的放射免疫测定及测定。

Endocrinologia japonica Pub Date : 1992-02-01 DOI: 10.1507/endocrj1954.39.81

H Yoshida, N Hamada, J Noh, K Ito, H Morii

{"title":"Radioimmunoassay for calcitonin gene-related peptide and its measurement in sera of patients with thyroid disease.","authors":"H Yoshida, N Hamada, J Noh, K Ito, H Morii","doi":"10.1507/endocrj1954.39.81","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.81","url":null,"abstract":"To investigate serum levels of calcitonin gene-related peptide (CGRP), we developed a sensitive radioimmunoassay (RIA). RIA for CGRP in serum can present some problems: the serum may degradate the tracer during incubation and suppress the antigen-antibody reaction. We avoided these problems by using aprotinin and CGRP-free serum instead of a buffer for the standard curve. We detected serum CGRP in all 39 healthy subjects when CGRP-free serum was not used for the standard curve, but 34 of these subjects had serum CGRP levels below the detection limit (less than 80 pmol/l) when CGRP-free serum was used for the standard curve. We defined the normal range for serum CGRP as below 100.8 pmol/l, which was the maximum level found in the healthy subjects. We studied serum levels of this peptide in patients with thyroid diseases, because the thyroid may be one origin of circulating CGRP. Four of 10 patients with medullary thyroid carcinoma had elevated serum levels of CGRP. Seven of 24 patients with subacute thyroiditis had elevated serum levels of CGRP, but at least one year after clinical recovery, CGRP was undetectable in all. Seven of the 37 patients with hypothyroidism had elevated serum levels of CGRP. None of the patients with hyperthyroidism, adenomatous goiter, thyroid adenoma, or thyroid carcinoma had elevated serum CGRP levels. It is necessary to use a standard curve obtained by the addition of aprotinin and CGRP-free serum to the assay standards to measure serum CGRP levels. Some patients with subacute thyroiditis, hypothyroidism, or medullary thyroid carcinoma had elevated serum CGRP levels.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 1","pages":"81-91"},"PeriodicalIF":0.0,"publicationDate":"1992-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.81","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12549633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Purification and properties of steroid sulfatase from human placenta. 人胎盘中类固醇硫酸酯酶的纯化及性质研究。

Endocrinologia japonica Pub Date : 1992-02-01 DOI: 10.1507/endocrj1954.39.93

T Suzuki, K Hirato, T Yanaihara, T Kadofuku, T Sato, M Hoshino, N Yanaihara

{"title":"Purification and properties of steroid sulfatase from human placenta.","authors":"T Suzuki, K Hirato, T Yanaihara, T Kadofuku, T Sato, M Hoshino, N Yanaihara","doi":"10.1507/endocrj1954.39.93","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.93","url":null,"abstract":"Steroid sulfatase was purified approximately 170-fold from normal human placental microsomes and properties of the enzyme were investigated. The major steps in the purification procedure included solubilization with Triton X-100, column chromatofocusing, and hydrophobic interaction chromatography on phenylsepharose CL-4B. The purified sulfatase showed a molecular weight of 500-600 kDa on HPLC gel filtration, whereas the enzyme migrated as a molecular mass of 73 kDa on sodium dodecyl sulfate polyacrylamide gel electrophoresis. The isoelectric point of steroid sulfatase was estimated to be 6.7 by isoelectric focusing in polyacrylamide gel in the presence of 2% Triton X-100. The addition of phosphatidylcholine did not enhance the enzyme activity in the placental microsomes obtained from two patients with placental sulfatase deficiency (PSD) after solubilization and chromatofocusing. This result indicates that PSD is the result of a defect in the enzyme rather than a defect in the membrane-enzyme structure. Amino acid analysis revealed that the purified human placental sulfatase did not contain cysteine residue. The Km and Vmax values of the steroid sulfatase for dehydroepiandrosterone sulfate (DHA-S) were 7.8 microM and 0.56 nmol/min, while those for estrone sulfate (E1-S) were 50.6 microM and 0.33 nmol/min, respectively. The results of the kinetic study suggest the substrate specificity of the purified enzyme, but further studies should be done with different substrates and inhibitors.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 1","pages":"93-101"},"PeriodicalIF":0.0,"publicationDate":"1992-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.93","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12773833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Serum intact parathyroid hormone concentration measured by a two-site immunoradiometric assay in normal subjects and patients with various parathyroid disorders. 两点免疫放射测定法测定正常受试者和各种甲状旁腺疾病患者血清完整甲状旁腺激素浓度。

Endocrinologia japonica Pub Date : 1992-02-01 DOI: 10.1507/endocrj1954.39.115

R Okazaki, T Matsumoto, Y Furukawa, Y Fujimoto, H Niimi, Y Seino, T Fujita, S Nagataki, E Ogata

{"title":"Serum intact parathyroid hormone concentration measured by a two-site immunoradiometric assay in normal subjects and patients with various parathyroid disorders.","authors":"R Okazaki, T Matsumoto, Y Furukawa, Y Fujimoto, H Niimi, Y Seino, T Fujita, S Nagataki, E Ogata","doi":"10.1507/endocrj1954.39.115","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.115","url":null,"abstract":"Serum intact parathyroid hormone (PTH) concentration was measured by a two-site immunoradiometric assay (IRMA) in normal subjects and patients with various parathyroid disorders. Serum intact PTH levels were all within the detection limit of the IRMA in normal subjects, and there was a significant negative correlation between serum calcium (Ca) and intact PTH levels. Although 3 out of 26 patients (11.5%) with primary hyperparathyroidism had a normal serum intact PTH concentration, these patients could be readily discriminated from normal subjects by plotting serum intact PTH against the serum Ca concentration. In contrast, serum intact PTH was undetectable in 16 out of 17 patients (94.1%) with idiopathic hypoparathyroidism. Patients with pseudohypoparathyroidism (PHP) type I, mostly under treatment with active vitamin D, exhibited wide distribution of serum intact PTH concentration, and appeared to belong to two distinct subgroups. One group of patients demonstrated a similar relationship between serum intact PTH and Ca levels to normal subjects. The other exhibited much higher serum intact PTH levels despite a normal serum Ca concentration, and no obvious relationship could be observed between the two parameters. These results demonstrate that an inverse relationship between serum Ca and intact PTH can be demonstrated in normal subjects with normocalcemia, that most of the parathyroid disorders can be diagnosed by measuring serum Ca and the intact PTH concentrations simultaneously, and that patients with PHP can be divided into two subgroups: one with a normal relationship between serum Ca and intact PTH, and the other with a high serum PTH level in the face of normocalcemia.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 1","pages":"115-20"},"PeriodicalIF":0.0,"publicationDate":"1992-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12773999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Abdominal vagotomy decreased the number of ova shed and serum progesterone levels on estrus in the cyclic hamster. 腹部迷走神经切开术降低了周期仓鼠发情时的排卵数和血清黄体酮水平。

Endocrinologia japonica Pub Date : 1992-02-01 DOI: 10.1507/endocrj1954.39.141

Y Nakamura, H Kato, P F Terranova

{"title":"Abdominal vagotomy decreased the number of ova shed and serum progesterone levels on estrus in the cyclic hamster.","authors":"Y Nakamura, H Kato, P F Terranova","doi":"10.1507/endocrj1954.39.141","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.141","url":null,"abstract":"The effects of abdominal vagotomy (AVGT) on ovarian function were studied in cyclic hamsters. AVGT significantly decreased the number of ova shed (AVGT: 10.5 +/- 1.5 ova/hamster, sham: 15.8 +/- 0.7 ova/hamster; P less than 0.05) and serum progesterone levels (AVGT: 2.1 +/- 0.3 ng/ml, sham: 3.9 +/- 0.7 ng/ml; P less than 0.05) on the morning of estrus. However, progesterone concentrations in the corpora lutea and non-luteal ovary on estrus in the AVGT and sham groups were similar. The serum estradiol levels in both groups on proestrus increased from 0900 h (AVGT: 75 +/- 10 pg/ml, sham: 72 +/- 6 pg/ml) to 1500 h (AVGT: 204 +/- 27 pg/ml, sham: 196 +/- 35 pg/ml) but there was no significant difference between the two groups. Partial degranulation of ovarian mast cells was not increased in the AVGT group. Also, vasoactive intestinal peptide (VIP) content in the ovary was not increased by AVGT at 0900 h on proestrus (AVGT: 60.1 +/- 16.8 pg/ovary, sham: 37.2 +/- 14.3 pg/ovary). These results indicated that AVGT interferes with normal ovulation and results in an increase in the number of atretic follicles, but that these effects by AVGT seemed not to be mediated through ovarian mast cells and VIP.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 1","pages":"141-5"},"PeriodicalIF":0.0,"publicationDate":"1992-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.141","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12773206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Western ligand blot assay for human growth hormone-dependent insulin-like growth factor binding protein (IGFBP-3): the serum levels in patients with classical growth hormone deficiency. Western配体印迹法测定人生长激素依赖性胰岛素样生长因子结合蛋白(IGFBP-3):典型生长激素缺乏症患者血清水平。

Endocrinologia japonica Pub Date : 1992-02-01 DOI: 10.1507/endocrj1954.39.121

Y Hasegawa, T Hasegawa, T Yokoyama, S Kotoh, Y Tsuchiya, F Kurimoto

引用次数: 11

Induction of ovulation and spermatogenesis by hMG/hCG in hypogonadotropic GH-deficient patients. 促性腺激素低下gh缺乏患者hMG/hCG诱导排卵和精子发生。

Endocrinologia japonica Pub Date : 1992-02-01 DOI: 10.1507/endocrj1954.39.31

Y Okada, T Kondo, S Okamoto, M Ogawa

{"title":"Induction of ovulation and spermatogenesis by hMG/hCG in hypogonadotropic GH-deficient patients.","authors":"Y Okada, T Kondo, S Okamoto, M Ogawa","doi":"10.1507/endocrj1954.39.31","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.31","url":null,"abstract":"Nine female and 20 male hypogonadotropic GH-deficient patients were studied for sexual development by hCG/hMG. In the female patients, gonadotropin therapy was started at the mean age of 22.7 +/- 2.1 years. The administration of progesterone induced withdrawal bleeding at an average of 2.77 +/- 1.94 years after the initiation of hMG/hCG therapy in 8 of the 9 patients studied. Of 6 patients who had been confirmed as positive in a gestagen test, induction of ovulation by hMG/hCG was observed in 5 patients at an average of 5.58 +/- 1.23 years after the onset of therapy, but not in the remaining patient who had been given estrogen and progesterone 4 years 9 months prior to the initiation of the gonadotropin therapy. In male patients, gonadotropin therapy was started at the mean age of 23.6 +/- 5.7 years. Seminal fluid was obtained by masturbation and brought to our clinic in the morning. Of the 20 patients, 19 patients could be observed once a month regularly. Of the 19 patients, spermatozoa could be detected at a mean period of 2.19 +/- 0.87 years after initiation of hCG/hMG therapy in 18, but not in the remaining patient, after 5 years of therapy, who did not receive hCG/hMG regularly. The sperm count exceeded 20 x 10(6)/ml and more in 12 and was lower than that in 8 patients after 3 years of the therapy. No side effects were observed in female patients, but gynecomastia developed in 2 of the 20 male patients. These data suggest that gonadotropin therapy for hypogonadotropic GH-deficient patients is effective in promoting ovulation and spermatogenesis despite the initial replacement therapy with sex hormones.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 1","pages":"31-43"},"PeriodicalIF":0.0,"publicationDate":"1992-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.31","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12773211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Effect of glucose on Na, K-ATPase activity in cultured bovine aortic endothelial cells. 葡萄糖对培养的牛主动脉内皮细胞Na、k - atp酶活性的影响。

Endocrinologia japonica Pub Date : 1992-02-01 DOI: 10.1507/endocrj1954.39.1

T Yamashita, F Umeda, T Hashimoto, T Inoguchi, T Yamauchi, K Mimura, J Watanabe, H Nawata

{"title":"Effect of glucose on Na, K-ATPase activity in cultured bovine aortic endothelial cells.","authors":"T Yamashita, F Umeda, T Hashimoto, T Inoguchi, T Yamauchi, K Mimura, J Watanabe, H Nawata","doi":"10.1507/endocrj1954.39.1","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.1","url":null,"abstract":"The effect of high concentrations of glucose on Na, K-ATPase activity and the polyol pathway was studied using cultured bovine aortic endothelial cells. Na, K-ATPase activity was expressed as ouabain-sensitive K+ uptake. A significant decrease in Na, K-ATPase activity with an intracellular accumulation of sorbitol was found in confluent endothelial cells incubated with 400 mg/dl glucose for 96 h. However, there was no significant change in the Na, K-ATPase activity or sorbitol content of the cells incubated with 100 mg/dl glucose plus 300 mg/dl mannitol. The decrease in Na, K-ATPase induced by the high glucose concentration was restored by the simultaneous addition of 10(-4) M ponalrestat (ICI 128,436; Statil), an aldose reductase inhibitor. The addition of this agent also significantly reduced the increase in sorbitol induced by high glucose levels. These results suggest that the decrease in Na, K-ATPase activity induced in cultured aortic endothelial cells by high concentrations of glucose may be caused in part by the accumulation of sorbitol.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"1992-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12492831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6