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Antibacterial Susceptibility Pattern of Gram Negative Bacteria Isolated from Patients with Urinary Tract Infection 尿路感染患者革兰氏阴性菌的抗菌药敏分析

Futuristic Biotechnology Pub Date : 2021-06-30 DOI: 10.54393/fbt.v1i01.1

H. Mukhtar

{"title":"Antibacterial Susceptibility Pattern of Gram Negative Bacteria Isolated from Patients with Urinary Tract Infection","authors":"H. Mukhtar","doi":"10.54393/fbt.v1i01.1","DOIUrl":"https://doi.org/10.54393/fbt.v1i01.1","url":null,"abstract":"In clinical setting, urinary tract infections (UTIs) rank second among infectious presentations. Each year, around 150 million people receive a UTI diagnosis worldwide. Objectives: To determine the antibacterial sensitivity of meropenem, imipenem and ciprofloxacin against bacteria isolated from patients presented with Urinary Tract Infection. Methods: This Descriptive type Cross-sectional study was carried out at Clinical Microbiology Laboratory, Pathology Department of King Edward Medical University. A total of 100 patients were included that had Urinary Tract Infections. Urine culture was done using standardized Cysteine lactose electrolyte deficient (CLED) agar. Gram reaction, morphology, and biochemical properties were used to identify bacterial pathogens. Antibiotic sensitivity of the selected Gram negative bacteria was observed by using the Kirby Bauer method for antibiotic susceptibility. Results: The bacteria isolated were E. coli (62%), Klebsiella spp. (11%), Candida spp. (8%), Pseudomonas aeruginosa (7%), Proteus spp. (4%), Staphylococcus spp. (3%), Staphylococcus saprophyticus (3%) and Enterobacter spp. (2%). Sensitivity pattern for Ciprofloxacin was 20 (23.3%) sensitive and 66 (76.7%) resistant. Similarly 87.2% were sensitive to Meropenem and 12.8% were resistant. While for Imipenem 95.3% were sensitive and 4.7% were resistant. Conclusions: It was concluded that UTI most commonly caused by E. coli which is a gram negative bacteria followed by Klebsiella, Pseudomonas, Proteus and Enterobacter. Other microorganisms such as Staphylococcus spp., Staphylococcus saprophyticus and Candida spp. were also isolated in least amount. Antimicrobial susceptibility showed that Gram negative isolates of uropathogens were highly resistant against Ciprofloxacin and showed sensitivity against Imipenem and Meropenem","PeriodicalId":114319,"journal":{"name":"Futuristic Biotechnology","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121386026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computer-Aided Drug Designing of Ocimum Basilicum Compounds as Therapeutic Agents Against RdRp of SARS-CoV2 罗勒复方抗SARS-CoV2 RdRp药物的计算机辅助药物设计

Futuristic Biotechnology Pub Date : 2021-02-26 DOI: 10.26434/CHEMRXIV.14069480.V1

M. Shafiq, R. Saif, L. Ali, T. Ahmad, A. Sohail

{"title":"Computer-Aided Drug Designing of Ocimum Basilicum Compounds as Therapeutic Agents Against RdRp of SARS-CoV2","authors":"M. Shafiq, R. Saif, L. Ali, T. Ahmad, A. Sohail","doi":"10.26434/CHEMRXIV.14069480.V1","DOIUrl":"https://doi.org/10.26434/CHEMRXIV.14069480.V1","url":null,"abstract":"The prevailing situation of the world is challenging due to COVID-19 pandemic that is caused by SARS-CoV2. To combat with this emerging pandemic by reducing disease severity and infection, the need of hour is to develop an effective vaccine and antiviral candidates as therapeutic agents against SARS-CoV2. This study was developed for the identification of potential anti-viral agents, from Ocimum basilicum against RdRp of SARS-CoV2. In this concern, nevadensin, ursolic acid, β-Sesquiphellandren, apigenin, nerolidol, nonyl acetate and geranyl acetate were screened out of fifty-seven compounds from Ocimum basilicum based on their best docking scores. The docking results were also compared with already clinically used drugs (Remdesivir and Ribavirin) against RdRp of SARS-CoV2. Molecular docking was performed using MOE software. The ADMET analysis and drug likeliness were also performed for all screened compounds by using admetSAR, pkCSM and SwissADME. Cumulatively, the optimum binding energies of screened compounds indicated their potential for drug development against SARS-CoV2. It appears promising that nevadensin exhibited a good docking score and high binding affinity towards RdRp of SARS-CoV2. Therefore, it may represent the potential to inhibit COVID-19. Hence, Ocimum basilicum nutraceuticals could be effective therapeutic candidates for the treatment and prevention of COVID-19.","PeriodicalId":114319,"journal":{"name":"Futuristic Biotechnology","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127689891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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