{"title":"Cytopathological Impacts of Certain Plant Growth Regulators on The Circulating Hemocytes of Galleria mellonella L. (Lepidoptera: Pyralidae)","authors":"Hamadah, Kh., G. K., El-Hela, A., A. A.","doi":"10.21608/eajbsa.2022.259860","DOIUrl":"https://doi.org/10.21608/eajbsa.2022.259860","url":null,"abstract":"The greater wax moth Galleria mellonella is a worldwide insect pest damaging wax combs and feeding on bee‐hive products. The objective of the current study was to investigate the cytopathological impacts of four plant growth regulators (PGRs), viz ., indole-3-acetic acid, indole-3-butyric acid, 2,4-Dichlorophenoxy acetic acid and 6-benzyladenine, on the circulating hemocytes of last (7 th ) instar larvae of G. mellonella . For this purpose, the 3 rd instar larvae were force-fed on diet supplemented with LC 50 values of these PGRs (0.24, 0.022, 0.16 & 0.085 ppm, respectively). These larvae were continuously fed on the treated diet throughout the larval stage. The important results could be summarized as follows. Five main types of the freely circulating hemocytes in the haemolymph of larvae had been identified as Prohemocytes (PRs), Plasmatocytes (PLs), Granulocytes (GRs), Spherulocytes (SPs) and Oenocytoids (OEs). Different qualitative disorders of the profile of each circulating hemocyte type were demonstrated in thelast instar larvae, such as destruction of cell nuclei and/or membrane, some extruded cytoplasmic contents, and production of some vacuoles in the cytoplasm. However, some of the tested PGRs failed to exhibit cytopathological impacts on certain hemocytes, since IAA and 6-BA failed to affect PLs, IBA and 6-BA failed to affect GRs, IBA and 6-BA failed to affect SPs and IBA and 6-BA failed to affect OEs. Therefore, IAA and 2,4-D may be recommended to use in the IPM program against G. mellonella","PeriodicalId":11424,"journal":{"name":"Egyptian Academic Journal of Biological Sciences. A, Entomology","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80645005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Agronomy, Laamari, Agronomy, Tahar-chaouche, Agronomy
{"title":"Pauesia silanaTremblay, 1969 A Parasitoid of Cinara maghrebica-Aphid in Aleppo Pine Forests in Algeria Case Khenchela Province","authors":"A. Agronomy, Laamari, Agronomy, Tahar-chaouche, Agronomy","doi":"10.21608/eajbsa.2022.259859","DOIUrl":"https://doi.org/10.21608/eajbsa.2022.259859","url":null,"abstract":"","PeriodicalId":11424,"journal":{"name":"Egyptian Academic Journal of Biological Sciences. A, Entomology","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72757535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Application of Entomopathogenic Nematodes to Control Larvae of Temnorhynchus baal (Reiche and Saulcy) Under Laboratory Conditions and In Strawberry Fields","authors":"D. Adly, G. Nouh, A. Eid","doi":"10.21608/eajbsa.2022.256736","DOIUrl":"https://doi.org/10.21608/eajbsa.2022.256736","url":null,"abstract":"The white grub, Temnorhynchus baal, is a major pest of the strawberry crop in many Asian and African countries. The white grabs cause significant economic losses in the yield. The present study aims to evaluate the effectiveness of entomopathogenic nematodes (EPNs) on the third larval instar of T. baal under laboratory conditions. The study extended to compare the efficacy of a combination of EPNs ( Steinernema glaseri + Heterorhabditis bacteriophora ) against a natural infestation of T. baal with an organophosphorus insecticide (Diazinon) in an Egyptian strawberry field. In the laboratory, five larvae of T. baal were placed in soil and infected with EPNs. At the highest concentration of 4000 IJs/larva, the larvae were more susceptible to infection with S. glaseri than H. bacteriophora , with mortality percentages of 96 and 88%, respectively. The LC50 of S. glaseri and H. bacteriophora was 937.44 and 1026.58 IJs/larvae, respectively. The mortality percentage was 96% and 100% when treated for five larvae/cup and one larva/cup, respectively, after being infected with a combination of S. glaseri + H. bacteriophora . The mortality percentage was higher in the mixture than for each species studied individually. In the field, throughout the seasons, the percentage of wilted plants was 17.64, 66.09, and 83.95% in 2020 and 12.56, 67.87, and 75.62% in 2021, for the plots treated with a combination of EPNs, insecticides, and control, respectively. Present findings indicate that entomopathogenic nematodes are good alternatives to control the white grub, T. baal , in strawberry fields.","PeriodicalId":11424,"journal":{"name":"Egyptian Academic Journal of Biological Sciences. A, Entomology","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86500386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative Amino Acid Content of Pseudocanthotermes grandiceps, Macrotermis jeanelli and Allodontermes tenax (Isoptera: Termitidea)","authors":"Makila Jacob, Omukunda Elizabeth, Ndong’a Millicent","doi":"10.21608/eajbsa.2022.256374","DOIUrl":"https://doi.org/10.21608/eajbsa.2022.256374","url":null,"abstract":"The main objective of this study was to compare the amino acid profiles of P.grandiceps, M. jeanelli and A. tenax. The study was carried out in Trans-Nzoia at an altitude of 1,900 meters, with a latitude of 1°1'8.72\"N, and longitude of 35°0'8.3\"E. Termite alates was collected when swarming and placed in 500grams collection jars and taken to the laboratory for amino acid analysis. Some of the alates and a number of small or large soldiers and workers were preserved in 80% ethanol for identification at the National Museums of Kenya. The collected termite alates were analyzed for composition of amino acids using standard methods detailed in AOAC (2000). From the results, there was no significant difference in amino acid content, amino acid classes, amino acid groups and total essential amino acids and total non-essential amino acids of the three termite species. Glutamic acid was the most abundant of the three species. It ranged between 8.43g/100g -15.5g/100g. The lowest concentrated amino acid was cysteine ranging between 0.60g/100g- 3.92g/100g. Leucine was found to be the most highly concentrated essential amino acid (eaa); 7.72g/100g crude protein in P. grandiceps , 7.48g/100g in M. jeanelli and 5.51g/100g in A. tenax . Methionine was the lowest eaa in P. grandiceps at 0.88g/100g crude protein while Histidine was the lowest eaa in both M. jeanelli and A. tenax at 2.15g/100g and 2.09g/100g respectively.","PeriodicalId":11424,"journal":{"name":"Egyptian Academic Journal of Biological Sciences. A, Entomology","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88679665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Study of the Colonization Behaviour of Stored Grains Pest, Sitophilus oryzae L. (Coleoptera: Curculionidae) Through Determining the Colour Preference.","authors":"A. M.","doi":"10.21608/eajbsa.2022.255521","DOIUrl":"https://doi.org/10.21608/eajbsa.2022.255521","url":null,"abstract":"Article History is The current research aimed to study the colour preferences of S . oryzae through the free choice test method, the preference of adult insects to different colour cues was evaluated with a multi-choice test 24 hours after insect release. The research was conducted with three replicates using six colour cues; red, white, black, yellow, green, and blue under laboratory conditions. The observed parameter was the number of adults attracted to the different colour cues. The results showed that the highest preference of S. oryzae was recorded in the green and red colour cues, while the white colour was the least preferred colour. These results could be helpful in the integrated pest management programs to control the stored product insect pest S . oryzae .","PeriodicalId":11424,"journal":{"name":"Egyptian Academic Journal of Biological Sciences. A, Entomology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88776290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eman E. Essa, Gawhara M. M. Abu El-Hassan, S. Farag
{"title":"Biochemical Composition, Toxicity and Bioactivities of the Essential Oil extracted from Coffea arabica L. husks against the Cotton Leafworm, Spodoptera littoralis (Boisduval) (Lepidoptera: Noctudiae)","authors":"Eman E. Essa, Gawhara M. M. Abu El-Hassan, S. Farag","doi":"10.21608/eajbsa.2022.254995","DOIUrl":"https://doi.org/10.21608/eajbsa.2022.254995","url":null,"abstract":"throughout Therefore, approaches for their a ) analyzed its were screened for insecticidal activity against the larval instars of the littoralis GC/MS analysis revealed that the arabic coffee included 13 compounds of which Oleic acid comprises the largest proportion 65 % which is known for its insecticidal efficacy. Other compounds that have insecticidal activities were also separated; Cyclononasiloxane, octadecamethyl-, n-Hexadecanoic acid, 9,12-Octadecadienoic acid (Z, Z)- and N-Isobutyl-11-(3,4-methylenedioxyphenyl)-2E,4E,10E-undecatrienoic amide. The oil extract was tested against the 4 th larval instars. The results indicated the efficacy of the extract as larvicide with the values of LC 50 and LC 90 equal to 1.8083 and 8.3227 respectively. The application of the oil extract against the larvae resulted in the prolongation of both larval and pupal duration and the decrease of the growth rate, pupation, adult longevity and fecundity. Also, the corrected weight of consumed leaf, consumption index (IC), relative growth rate (RGR), the efficacy of ingested and digested food conversion to body tissue (ECI) and (ECD), respectively and the approximate digestibility (AD) were decreased after the application of the oil extract on the larvae. As a result of the current study, the oil extract from the husk of Coffea arabica revealed its efficacy as both larvicide and deterrent against the 4 th instar larvae of cotton leafworm S. littoralis .","PeriodicalId":11424,"journal":{"name":"Egyptian Academic Journal of Biological Sciences. A, Entomology","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86704764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Effect of Onion Powder (Allium Sp.) As An Antibiotic on The Larval Haemolymph of Silkworm and Its Effect on The Economic Parameters of Cocoons as A Food Supplementary","authors":"Shimaa, Shakl","doi":"10.21608/eajbsa.2022.251819","DOIUrl":"https://doi.org/10.21608/eajbsa.2022.251819","url":null,"abstract":"The study aims to use natural antimicrobial such as onion powder ( Allium sp .) which has an effective role as a strong antibiotic to raise the immunity of sensitive silkworm larvae to infection with many diseases. Three doses of Onion powder were used 5,10 and 15gm, and the following was studied: The economic parameters of the cocoons and the weight of silkworm larvae of the fifth age and the effect of using the onion powder on these traits were studied. The results showed that the maximum weight of the larvae was in the dose of 15 gm while the dose of 10 gm was the highly increment in the weight of cocoon, cocoon shell weight and the shell ratio. It concluded that the use of 5 and 10gm from Onion Powder as supplementary feeding on the mulberry leaves enhanced the immunology of larvae without affecting the quality of the economic parameters of the cocoons.","PeriodicalId":11424,"journal":{"name":"Egyptian Academic Journal of Biological Sciences. A, Entomology","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76125427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Histological Changes in the Adult Seed Bug, Graptostethus servus (Hemiptera: Lygaeidae) Treated with the Entomopathogenic Fungus, Beauveria bassiana (Ascomycota: Hypocreales).","authors":"M. Nada, A. Gad, A. Soliman","doi":"10.21608/eajbsa.2022.251638","DOIUrl":"https://doi.org/10.21608/eajbsa.2022.251638","url":null,"abstract":"---------------------Keywords is very vulnerable to B. bassiana. LC 50 value was 2.9x10 6 spores/ml and the LT 50 value was 4.16 days. The infection affected various body parts, especially the cuticle and the midgut. The application of B. bassiana on the adultʼs cuticle resulted in an apparent disturbance in the structure of the cuticle and degeneration of its different parts. These changes were observed 48 hours after treatment and increased in severity over time. The fungal infection was not limited to the cuticle, but also affected the epithelial cells of the crop and midgut, as well as the mesenteric caeca. These results highlight the insecticidal potential of the fungus B. bassiana against the pest G. servus .","PeriodicalId":11424,"journal":{"name":"Egyptian Academic Journal of Biological Sciences. A, Entomology","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77154242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"New Record of The Billbug Genus Sphenophorus Schoenherr, 1838 (Coleoptera: Curculionidae: Dryophthorinae) From Egypt","authors":"Waleed El-Maghraby, Hala Kadada, Manal Elshaier","doi":"10.21608/eajbsa.2022.249408","DOIUrl":"https://doi.org/10.21608/eajbsa.2022.249408","url":null,"abstract":"the specimen is billbug species of the genus Sphenophorus but they cannot confirm the species. During the present work, the specimens were described, photographed, and identified using the available taxonomic keys and previous descriptions. The taxonomic work confirmed that the specimens belong to Sphenophorus venatus Chittenden (hunting billbug) and were reported for the first time from Egypt. This finding with data about damage, distribution, and host plants contributes to enriching lists of Egypt’s insect fauna and conservation of biodiversity.","PeriodicalId":11424,"journal":{"name":"Egyptian Academic Journal of Biological Sciences. A, Entomology","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88881278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Lydia, H. Berrai, A. Abdul-Hussain, L. Saharaoui, M. Biche
{"title":"First Records of Wahlgreniella nervata (Gillette, 1908) (Hemiptera : Aphididae) on Bell Pepper In The Region of Staoueli (Algeria)","authors":"D. Lydia, H. Berrai, A. Abdul-Hussain, L. Saharaoui, M. Biche","doi":"10.21608/eajbsa.2022.249311","DOIUrl":"https://doi.org/10.21608/eajbsa.2022.249311","url":null,"abstract":"","PeriodicalId":11424,"journal":{"name":"Egyptian Academic Journal of Biological Sciences. A, Entomology","volume":"99 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72430088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}