S. Hazem, Rania I. Ismail, Hend I. Elsharkawy, Mohamed K. Al-kholi, Eman M. Younis, Saher A. El-Madawy
{"title":"Overview on Brucellosis in Camels","authors":"S. Hazem, Rania I. Ismail, Hend I. Elsharkawy, Mohamed K. Al-kholi, Eman M. Younis, Saher A. El-Madawy","doi":"10.21608/ejah.2023.314382","DOIUrl":"https://doi.org/10.21608/ejah.2023.314382","url":null,"abstract":"A total of 270 dromedary dairy camels above five years old with a history of reproductive disorders, including abortion, repeated breeding, reduced milk production, and retained fetal membranes belonging to different localities at El-Sharqia Governorate, including Abu Kabir, Belbis, DeyarbNegm, EL Husseiniya, and Minya al-Qamh were employed in this study. Different risk factors associated with camel brucellosis were investigated in these camel's populations, these include the rearing of camels with different ruminant’s species, addition of new animals, as well as lack of sanitary measures. The modified Rose Bengal Test and Complement Fixation Test revealed a prevalence of 40 (14.8%) and 32 (11.9%) respectively. The three districts with the highest prevalence of camel brucellosis were Belbeis (14.12%), EL Husseiniya (12.19%), and Minya al-Qamh (10.7%). Three (9.4%) brucella species were isolated out of 32 milk samples from serologically positive she-camels. Two isolates were identified as B. melitensis biovar 3 and one isolate was identified as B. abortus biovar1. Bru-cella strain DNA extracts from milk samples showed a specific amplicon of 731 bp specific for B. melitensis (18) and an amplicon of 498 bp specific for B. abortus (4), but no amplicon of 285 bp specific for B. suis could be detected after being subjected to PCR using the IS711 primer in this investigation. We concluded that, in this study, Brucella meliteneis biovar 3 is a common field strain in camels. Oxidative stress biomarkers, malonaldehyde (MDA) were increased significantly ( P ≤ 0.05) in infected camel, whereas nitric oxide (NO) was moderate increase. Antioxidant enzymes like Gluta-thione Peroxidase enzyme (GPx) was significantly increase whereas catalase (CAT) was significantly reduced in infected camel. This study has confirmed our understanding of risk factors associated with camel brucellosis. The rearing of camels with different ruminant’s species, the addition of new animals, and the lack of sanitary measures were the primary risk factors associated with the spread of brucellosis among dairy camels .","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91369053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Beni-Suef Governorate, H. Ghada, Ali, M. Emtenan, Hassan
{"title":"Incidence of aflatoxin and ochratoxin in some poultry ration with trial for control in Beni-Suef Governorate.","authors":"Beni-Suef Governorate, H. Ghada, Ali, M. Emtenan, Hassan","doi":"10.21608/ejah.2023.311501","DOIUrl":"https://doi.org/10.21608/ejah.2023.311501","url":null,"abstract":": A total of 60 samples (30 poultry feed and 30 chicken liver samples) were collected randomly from some farms and localities in Beni-Suef Governorate in summer and winter seasons for monitoring my-cotoxin contamination condition in such farms. Samples were examined for aflatoxin and ochratoxin levels. All samples (poultry feed and chicken liver samples) were found contaminated with both mycotoxins. Concerning , poultry feed samples in summer, aflatoxin levels ranged from 1.10 to 65 ppb with mean of 8.79±4.11, one sample (6.66%) exceeded the permissible limit (20 ppb); While, ochratoxin levels ranged from 3.50 to 9.90 ppb with mean of 5.51±0.52, eight samples (53.33%) exceeded the permissible limit (5 ppb). In winter, aflatoxin levels ranged from 0.15 to 7.70 ppb with mean of 3.01±0.93, all samples were within the permissible limit (20 ppb); while, ochratoxin levels ranged from 2.50 to 11.90 ppb with mean of 4.55±0.70, four samples (26.66%) exceeded the permissible limit (5 ppb). Chicken liver samples were found contaminated with aflatoxin levels ranged from 0.51 to 6.52 ppb with mean of 2.15±0.72, all these levels were within FDA permissible limit (20 ppb); nine samples (30%) exceeded FAO permissible limits (4 ppb). While, ochratoxin levels ranged from 1.90 to 5.40 ppb with mean of 2.55±0.32, three samples","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81952892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rabab T. Hassanien, H. Hussein, M. H. Ali, M. Shahein
{"title":"Overview of West Nile Virus","authors":"Rabab T. Hassanien, H. Hussein, M. H. Ali, M. Shahein","doi":"10.21608/ejah.2023.305227","DOIUrl":"https://doi.org/10.21608/ejah.2023.305227","url":null,"abstract":"F laviviruses had an extensive spread around the universe. West Nile Virus (WNV) is a zoonotic virus that threatens health of humans and animals and causes several epidemics for both hosts. Additionally, it has economic losses due to the expense of treatment of diseased cases, control programs, and animal losses. The epidemic spillover and spreading of the viruses is geographically distinguished in the last few decades. The epidemiological features of WNV are controlled by its insect vectors, their geo-graphical distribution, climatic changes, and changes in arthropods' habitat as a consequence of the change in urbanization behavior as well as the global travel movements. National and international surveillance for WNV transmission is an important strategy to track the virus's spread and effective management methods.","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"84 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82880449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparing real-time RT-PCR to the conventional method for titration of live attenuated avian infectious bronchitis vaccine","authors":"","doi":"10.21608/ejah.2023.296096","DOIUrl":"https://doi.org/10.21608/ejah.2023.296096","url":null,"abstract":"","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87469027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bacteriological Evaluation of some meat products from different markets in El-Behera Governorate","authors":"","doi":"10.21608/ejah.2023.294692","DOIUrl":"https://doi.org/10.21608/ejah.2023.294692","url":null,"abstract":"","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81025420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Samy, M. Said, A. H. Mohamed, A. Halim, Mohamed A. Soliman, Moataz Mohamed
{"title":"Comparison of replication competence between two highly pathogenic avian influenza viruses H5N1 and H5N8 in vitro","authors":"M. Samy, M. Said, A. H. Mohamed, A. Halim, Mohamed A. Soliman, Moataz Mohamed","doi":"10.21608/ejah.2023.294051","DOIUrl":"https://doi.org/10.21608/ejah.2023.294051","url":null,"abstract":"ixed infections with respiratory viruses have become frequent at poultry farm business in Egypt. The pandemic of highly pathogenic avian influenza virus (HPAIV) H5N1 (clade 2.2.1.2) has been spreading through the country since 2006. In 2016, the Spread of HPAIV H5N8 (clade 2.3.4.4b) to Egypt via wild birds into commercial poultry flocks made overburden on the economic situation of poultry business. The surveillance efforts conducted by the governmental veterinary authority GOVS showed dominating infection of H5N8 with rare spread of H5N1 in poultry market sector. Thus, the current study try to figure out whether a replication competence exhibited between the two highly pathogenic avian influenza viruses HPAIV H5N1 and H5N8 through designation an in-vitro induced infection of H5N8 and H5N1 in specific pathogen free embryonated chicken eggs (SPF-ECE). A concentration of 10 3 EID50 of HPAIV H5N1 and H5N8 were either sequentially or simultaneously inoculated into the allantoic cavity of SPF-ECE at 48 h of incubation, followed by the collection of allantoic fluid. A quantitative reverse transcription real-time polymerase chain reaction was used to determine the quantitative replication of viral RNA copies of neuraminidase gene of both AIV strains. A replication competence was observed according to which viral strain firstly inoculated. Whereas, H5N1 RNA titers was reduced by ≥4log 10 when H5N8 firstly inoculated. Vice versa H5N8 RNA titers were reduced by ≥4log 10 when H5N1 firstly inoculated. The interference","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84966216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Detection of some virulence and antibiotics resistance genes in Campylobacter isolated from turkeys","authors":"","doi":"10.21608/ejah.2023.293668","DOIUrl":"https://doi.org/10.21608/ejah.2023.293668","url":null,"abstract":"","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"82 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75883032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Validation of Simple In vitro alkaline DNA Comet assay for detection and evaluation of Genotoxicity and Biosafety of inactivated veterinary vaccines on Peripheral blood cell lymphocytes","authors":"","doi":"10.21608/ejah.2023.294819","DOIUrl":"https://doi.org/10.21608/ejah.2023.294819","url":null,"abstract":"","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85386143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Influence of Different Routes of Administration on the pharmacokinetic aspects of Doxycycline in Turkey","authors":"Ahmed M. Elmahdy, Mona Abd-Allah, Abd Elrehim","doi":"10.21608/ejah.2023.291740","DOIUrl":"https://doi.org/10.21608/ejah.2023.291740","url":null,"abstract":".","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87020616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Microbiological and pathological studies of concurrent infections associated with retarded growth in broilers at Behira governorate","authors":"","doi":"10.21608/ejah.2023.296094","DOIUrl":"https://doi.org/10.21608/ejah.2023.296094","url":null,"abstract":"","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75319241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}