Egyptian Journal of Animal Health最新文献_第10页

Effect of dietry Origanum vulgare extract on Immunological responses of Basa fish (Pangasius hypophthalmus) against Aeromonas hydrophila infection. 土豆泥提取物对巴沙鱼对嗜水气单胞菌免疫反应的影响。

Egyptian Journal of Animal Health Pub Date : 2022-04-01 DOI: 10.21608/ejah.2022.240044

A. Abd-elaziz, Safaa H. Aboolo

{"title":"Effect of dietry Origanum vulgare extract on Immunological responses of Basa fish (Pangasius hypophthalmus) against Aeromonas hydrophila infection.","authors":"A. Abd-elaziz, Safaa H. Aboolo","doi":"10.21608/ejah.2022.240044","DOIUrl":"https://doi.org/10.21608/ejah.2022.240044","url":null,"abstract":"O riganum vulgare essential oil (OEO) was added in diets of Basa fish ( Pangasius hypophthalmus ) at levels of 0.0, 5.0, 10.0, 15.0, and 20.0 g /kg feed to determine its effects on antioxidative capacity, immunity, histopathology, expression of immune-related genes and resistance to Aeromonas hydrophila . After 2 months of feeding experiment, serum lysozyme, superoxide dismutase and catalase enzymes have been dramatically increased according to the levels of added (OEO) , the highest level was at group fed on 20 gm /kg feed and 15gm/kg feed while, the lowest level at the control group also the expression of interleukin-1beta and toll-like receptor 2 genes in kidney tissues have been increased as the same manner .OEO-supplemented diet pereserves the normal architecture of liver, gills, intes-tines, and kidneys in all experimental groups. The scores of the histopathological lesions were significantly decreased with presence of regeneration to damaged cells in groups fed 20 gm OEO/kg feed &15 gm OEO/kg feed. After pathogenic A. hydrophila challenge the mortality percent after 10 days of the challenge experiment promptly reduced and the percent of survival of Basa fish was relatively increased by OEO administration according to the given dose . Findings show that the OEO in diet has improved the antioxidative status, immunological responses, histopathology, evaluation of immune related genes expression of Basa","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86778277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacteriological and molecular characterization of Brucella isolates from sheep and goats 绵羊和山羊布鲁氏菌分离株的细菌学和分子特性

Egyptian Journal of Animal Health Pub Date : 2022-04-01 DOI: 10.21608/ejah.2022.252288

Eman I. M. Beleta, Rania I. Ismail, Hend I. Elsharkawy

{"title":"Bacteriological and molecular characterization of Brucella isolates from sheep and goats","authors":"Eman I. M. Beleta, Rania I. Ismail, Hend I. Elsharkawy","doi":"10.21608/ejah.2022.252288","DOIUrl":"https://doi.org/10.21608/ejah.2022.252288","url":null,"abstract":"T his study reported on a sporadic, naturally acquired infection of sheep and goats with Brucella on a private farm in El- Menofya Gover-norate, Egypt. The abortions, which occurred in a flock of 46 sheep and 33 goats, involved six ewes and five does at the third month of gesta-tion. Serum samples from the flock were examined for Brucella antibodies using rose bengal test (RBT), Tube Agglutination Test (TAT) , indirect Enzyme Linked Immunosorbent Assay (I ELISA) and were further confirmed using complement fixation test (CFT) gave 18(39.1%), 16(34.8%), 19 (41.3%), and 17(37%) in sheep; respectively, while in goats revealed 11 (33.3%), 8(24.2%), 12(36.4%), and 11(33.3%) respectively. Tissue samples were collected from 28 positive animals, as detected by CFT, at slaughter-ing. The bacteriological results revealed 44 isolates which were biochemi-cally identified as B. melitensis biovar3. The highest recovery rate was obtained from supra mammary lymph nodes (22/28; 78.6%), followed by spleen (15/28; 53.6%), and finally liver (7/28; 25%).The distribution of the virulence genes among 44 B. melitensis isolates revealed that Omp 25 recorded the highest incidence 44(100%), then followed wbkA 43(97.7%) and manB 42 (95.5%) . The high prevalence of virulence-associated genes among the B. melitensis","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88961667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring the antimicrobial resistance of Salmonella and Escherichia coli in chickens and migratory birds 鸡和候鸟中沙门氏菌和大肠杆菌的耐药性监测

Egyptian Journal of Animal Health Pub Date : 2022-04-01 DOI: 10.21608/ejah.2022.246348

引用次数: 0

Usage of concentrated pomegranate to extend the shelf life of chicken breast 用浓缩石榴延长鸡胸肉的保质期

Egyptian Journal of Animal Health Pub Date : 2022-04-01 DOI: 10.21608/ejah.2022.240650

El Asuoty, M. Gerges

引用次数: 0

Antibiotic resistance pattern of Clostridium perfringens isolated from necrotic enteritis cases of broiler chickens in Luxor city 卢克索市肉鸡坏死性肠炎产气荚膜梭菌的耐药性分析

Egyptian Journal of Animal Health Pub Date : 2022-04-01 DOI: 10.21608/ejah.2022.252183

S. El-Nagar, Azhar G. Shalaby, H. Badr

{"title":"Antibiotic resistance pattern of Clostridium perfringens isolated from necrotic enteritis cases of broiler chickens in Luxor city","authors":"S. El-Nagar, Azhar G. Shalaby, H. Badr","doi":"10.21608/ejah.2022.252183","DOIUrl":"https://doi.org/10.21608/ejah.2022.252183","url":null,"abstract":": C lostridium perfringens is one of the highest dominant spore-forming foodborne pathogens, which is widely distributed and causes severe diseases in humans and animals. Poultry is the main host of this pathogen. In this study, we investigated the prevalence, antibiotic resistance pattern, and toxin-encoding genes of C. perfringens isolated from chicken with necrotic enteritis. Twelve of C. perfringens strains were isolated from 100 intestinal samples (Native and Hubbard chickens) with a percentage of 12% and subsequently confirmed by PCR; all strains carried alpha-toxin gene and were negative for other major toxin genes (Beta, Epsilon and Iota). The C. perfringens isolates showed highly resistance (100%) to most of the antibiotics tested; Ampicillin, Amoxicillin, Amoxicillin-clavulanic acid, Erythromycin, Lincomycin, Metronidazole, Neomycin, Oxytetracycline, Penicillin, Spectinomycin, Streptomycin, but showed sensitivity with a low percentage (25% or low) for Colistin sulphate, Doxycyclin, Enrofloxacin, Norfloxacin and Sulfamethoxazole-trimethoprim. However, virulence genes were detected with a different percentage of 100%, 75% and 16.7 with Cpe , netB and Tpel respectively. In the examination of antimicrobial-resistant genes the examined isolates showed 100%, 75% and 25%","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83028071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Brucella infection in dairy buffaloes using conventional and molecular methods 常规方法与分子方法检测奶牛布鲁氏菌感染

Egyptian Journal of Animal Health Pub Date : 2022-04-01 DOI: 10.21608/ejah.2022.236770

S. Hazem, Rania I. Ismail, Hend I. Elsharkawy, Eman I. M. Beleta, Abeer A. E. Mohamed

{"title":"Detection of Brucella infection in dairy buffaloes using conventional and molecular methods","authors":"S. Hazem, Rania I. Ismail, Hend I. Elsharkawy, Eman I. M. Beleta, Abeer A. E. Mohamed","doi":"10.21608/ejah.2022.236770","DOIUrl":"https://doi.org/10.21608/ejah.2022.236770","url":null,"abstract":"T his study was conducted on different dairy buffalo herds. A total of 316 buffaloes including 290 buffaloes suffering from reproductive disorders and 26 apparently healthy buffaloes in different rural areas belonging to the Al-Qalyubia Governorate were employed in this study. Epidemiological data revealed that these animals had no history of vaccination against Brucellosis. Blood sera of these animals were subjected to serological assessments using the Rose Bengal plate test (RBPT), Competitive En-zyme Linked Immunosorbent Assay (cELISA), complement fixation test (CFT) in addition to the use of real-time PCR ( rtPCR). MRT and bacteriological examination were performed on milk samples. The obtained results showed that the overall sero-prevalences of brucellosis were recorded as 17.41% , 17.09 % , 16.77% , and 17.09% by RBT, c-ELISA, CFT and rtPCR respectively. B. melitensis biovar 3 was isolated from milk samples 47 (14.87%) It was concluded that for the diagnosis of brucellosis in dairy buffaloes, MRT and RBT remain recommended for screening, CFT is ad-vised for confirming infection in individual animals, and cELISA can be used instead of CFT in some cases. Using rtPCR on serum samples should be regarded as an additional diagnostic technique for detecting and identify-ing Brucella infection and should be considered in conjunction with other serological tests that can progress and conquer the limits of different immunoassays. According to bacteriological testing, B. melitensis biovar 3 is still the prevalent Brucella field strain","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80160067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Some hormonal changes related to some trace elements deficiency in ration of male lambs 雄性羔羊日粮中微量元素缺乏与激素变化有关

Egyptian Journal of Animal Health Pub Date : 2022-01-01 DOI: 10.21608/ejah.2022.220757

Hanan A. Tag El Din, Hamada M. Yousif, Reda Rezk

{"title":"Some hormonal changes related to some trace elements deficiency in ration of male lambs","authors":"Hanan A. Tag El Din, Hamada M. Yousif, Reda Rezk","doi":"10.21608/ejah.2022.220757","DOIUrl":"https://doi.org/10.21608/ejah.2022.220757","url":null,"abstract":"T race element deficiency in male lambs is a serious problem. It refers to either insufficient nutrients or mineral elements imbalance. This work aimed to investigate the hormonal and some biochemical changes related to some trace element deficiency and the effect of mineral mixture supplementation on these changes. To achieve this aim we induced trace element deficiency in a tested male lambs group (N=10) belonged to our sheep herd and received a mineral mixture as a feed supplement for 10 days. The analysis of tested diet revealed protein 12.32%, calcium 1.21%, phosphorus 0.27%, and zinc 25.96 mg/kg DM. While the control healthy group (N=10) received a traditional diet. The results of serum hormones and biochemical analysis of tested group revealed a significant (P˂0.05) decrease in TSH, T3, T3/T4, testosterone, free testosterone, SHBG, GH, albumin and zinc, with a significant (P˂0.05) increase in T4, total protein and globulin compared to control healthy group. On the other hand, the results obtained after mineral mixture supplementation revealed a significant (P˂0.05) increase of TSH, T3, T3/T4, testosterone, free testosterone, SHBG, GH, albumin and zinc. Also, a significant (P˂0.05) decrease in T4, total protein, and globulin was observed compared with this group before mineral mixture supplementation, with observation of the changes were gradually toward recovery. Moreover, insignificant changes were recorded in calcium, phosphorus and iodine. In conclusion to trace element deficiency have an effect on vital hormones with extension to metabolic process, general health condition and proper growth of male lambs.","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83172881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of Spirulina-Platensis against Nano-copper Toxicity in Rabbit 螺旋藻对家兔纳米铜毒性的影响

Egyptian Journal of Animal Health Pub Date : 2022-01-01 DOI: 10.21608/ejah.2022.225148

Ehdaa, O. Hamed, A. Zohree

引用次数: 0

Effect Of Amprolium and Toltrazuril On disposition kinetics Of Tylvalosin In Infected Broiler Chickens With Mycoplasma Gallisepticum 氨丙铵和托曲祖利对鸡链球菌支原体感染后泰洛菌素处置动力学的影响

Egyptian Journal of Animal Health Pub Date : 2022-01-01 DOI: 10.21608/ejah.2022.226583

Ahmed R. E. Mahdy, Zinab Mohamed

{"title":"Effect Of Amprolium and Toltrazuril On disposition kinetics Of Tylvalosin In Infected Broiler Chickens With Mycoplasma Gallisepticum","authors":"Ahmed R. E. Mahdy, Zinab Mohamed","doi":"10.21608/ejah.2022.226583","DOIUrl":"https://doi.org/10.21608/ejah.2022.226583","url":null,"abstract":"A fter a single oral treatment, the pharmacokinetic characteristics of tylvalosin alone and in combination with either amprolium or toltrazuril in Mycoplasma gallisepticum-infected broiler chickens were evaluated. 18 chickens were divided into 3 equal groups, group one was given tylvalosin only, group two was given tylvalosin and amprolium and group three was given tylvalosin and toltrazuril. The best model for describing tylvalosin serum levels in our study was found to be a twocompartment open model. The maximum serum concentration of tylvalosin was 1.62±0.03, 1.2±0.02, and 1.41±0.01 μg/ml reached at 2.95±0.014, 2.8±0.01, and 2.83±0.02 h in group one, group two, and group three respectively. A significant (P<0.001)increasesin V/F and Cl/F10.19±0.11(mg)/(μg/ ml), 1.54±0.04(mg)/(μg/ml)/h and 8.56±0.07(mg)/(μg/ml), 1.31±0.03 (mg)/ (μg/ml)/h were found in group two and group three, respectively in comparison with group one 7.24 ± 0.06 (mg)/(μg/ml), 1.09±0.02 (mg)/(μg/ml)/h. The absorption and elimination half-lives of tylvalosin were 1.69 ± 0.016 h, 10.63± 0.330h and 1.56± 0.01h, 9.63± 0.41h and 1.58±0.02 h, 9.64±0.35 h in group one, group two, and group three respectively. The area under the curve was significantly (P < 0.001) decreased in group two and group three which were 13.6±0.06 and 15.98±0.14 μg/ml/h respectively, in comparison with group one which was 18.7±0.093 μg/ml/h. It was found that the serum concentration of tylvalosin in group one after twenty-four hours is higher than MIC for the used Mycoplasma gallisepticum (0.25 μg/ml) unlike the second and third group, so it is recommended to give tylvalosin every twenty-four hours if it is given alone while when it is given with amprolium and toltrazuril it should be given every twelve hours.","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87104559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacteriological and Molecular Studies on Johne’s Disease in Cattle 牛约翰氏病的细菌学和分子研究

Egyptian Journal of Animal Health Pub Date : 2022-01-01 DOI: 10.21608/ejah.2022.230532

El-Gedawy, Yousry A. El-Shazly

{"title":"Bacteriological and Molecular Studies on Johne’s Disease in Cattle","authors":"El-Gedawy, Yousry A. El-Shazly","doi":"10.21608/ejah.2022.230532","DOIUrl":"https://doi.org/10.21608/ejah.2022.230532","url":null,"abstract":"P aratuberculosis or Johne’s Disease (JD) is a chronic and incurable granulomatous enteric disease affecting cattle, buffaloes, sheep, goats and the other ruminants caused by M. avium subspecies paratuberculosis (MAP). In this study, A total of 300 cows (240 clinically diseased animals and 60 apparently healthy animals) were examined for the presence of clinical signs of johne's disease including incurable chronic diarrhoea, interment firstly then intense and continuous which is not responding to treatment, emaciation and progressive weakness. Fecal and serum samples (each of 300) were collected from the examined cows housed in 3 Egyptian Governorates (Sharkia, Kalyoubia and Giza). Fecal samples were collected then examined according to the pooling procedure and decontaminated by Hexa decylpyridinium chloride solution (HPC 0.9% prior to culturing on Herrold's Egg Yolk Medium (HEYM). MAP was isolated from 34 of the 60 pooled fecal samples tested (57%). Fecal smears were examined using Ziehl- Neelsen stain (ZN) for the presence of acid fast bacilli revealing 29 fecal smears (48%) of 60 fecal smears were positive. ELISA was conducted on serum samples to detect antibodies against MAP, 212 (71%) of serum samples were positive for antibodies against MAP. Molecular confirmation by PCR IS900 assay was carried out using specific primers directly on fecal sample, Out of the 60 pooled fecal samples, 45 pools (75%) were positive. A phylogenetic analysis for determining the genetic difference between current infection and other infected strains in other localities","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76449005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0