DNA Sequence最新文献_第6页

A 6940 bp DNA Fragment from Desulfovibrio gigas Contains Genes Coding for Lipoproteins, Universal Stress Response and Transcriptional Regulator Protein Homologues 一个6940 bp的DNA片段包含脂蛋白、通用应激反应和转录调节蛋白同源物的编码基因

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109024997

Gabriela Silva, C. Rodrigues-Pousada

{"title":"A 6940 bp DNA Fragment from Desulfovibrio gigas Contains Genes Coding for Lipoproteins, Universal Stress Response and Transcriptional Regulator Protein Homologues","authors":"Gabriela Silva, C. Rodrigues-Pousada","doi":"10.3109/10425170109024997","DOIUrl":"https://doi.org/10.3109/10425170109024997","url":null,"abstract":"The nucleotide sequence of a 6940 bp DNA fragment from Desulfovibrio gigas, containing seven ORFs was determined. ORF-1 encodes a probable lipoprotein having high similarities with lytic transglycosylases. ORF-2 encodes a polypeptide that does not show homologies with proteins deposited in the database, but it contains the consensus pattern of class II aminoacyl-tRNA synthetases. The putative protein encoded by ORF-3 possesses high similarities with universal stress response proteins from the UspA family. Northern blot analysis of ORF-3 shows that it is constitutively and abundantly expressed. ORF-4 encodes a probable helix-turn-helix-containing DNA-binding protein, given the presence of the helix-turn-helix motif, characteristic of this class of proteins. Its N-terminal region has high identity with its counterparts from proteins belonging to the RRF2 family. Northern blot analysis shows that ORF-4 is transcribed as a single mRNA in contrast to its orthologue from Desulfovibrio vulgaris. ORF-5 encodes a putative fusion protein as its N- and C-termini show significant homologies with molybdenum formylmethanofuran dehydrogenase and molybdopterin biosynthesis proteins, respectively. ORF-7 encodes a prokaryotic lipoprotein having homologies with multidrug efflux and DNA damage-inducible proteins, and it is constitutively and abundantly expressed.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"18 1","pages":"229 - 238"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83824918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Cloning and Characterization of 5′-Upstream Sequence of the M32 Gene for a Mouse Homologue of Drosophila Heterochromatin Protein 1 (HP1) 果蝇异染色质蛋白1 (HP1)同源物M32基因5′上游序列的克隆与鉴定

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109047562

M. Sato, K. Miyado, M. Kimura

{"title":"Cloning and Characterization of 5′-Upstream Sequence of the M32 Gene for a Mouse Homologue of Drosophila Heterochromatin Protein 1 (HP1)","authors":"M. Sato, K. Miyado, M. Kimura","doi":"10.3109/10425170109047562","DOIUrl":"https://doi.org/10.3109/10425170109047562","url":null,"abstract":"M32 [also termed chromatin modifier protein 2 (MOD2)] is a nuclear protein consisting of the condensed chromatin structure (heterochromatin) and considered one of the mammalian homologues of heterochromatin protein 1 (HP1), first isolated as one of the components of heterochromatin in Drosophila. This report presents the isolation and characterization of the 5′-upstream region of the mouse M32 gene containing a promoter region and 5′-untranslated region (5′-UTR) exon. The 5′-upstream region (approximately 0.27 kb starting from the 5′ end of the 5′-UTR exon) of the M32 gene contained neither a TATA box nor a CCAAT box, but possessed potential binding sites for transcription factors such as Spl, H4TF-1, PEA2, PEA3, GSG element and Egr-1, and was highly G/C-rich. The promoter activity of this 5′-upstream region was demonstrated by transfecting its fusion-construct with the E. coli β-galactosidase gene into the F9 mouse teratocarcinoma cell line. The 5′ ends of the mRNA were mapped to at least two positions in the 5′-upstream region. Interestingly, the 5′-upstream region exhibited a high degree of similarity to a portion of heterogeneous nuclear ribonucleo-protein (hnRNP) A2/B1 gene, which is thought to play a role in RNA processing, located in the reverse orientation to the M32 gene, and also to several known ESTs and cDNAs. These findings suggest that the 5′-upstream region of the M32 gene consists of a multiple regulatory complex which probably plays important roles in nuclear function such as chromatin organization and RNA processing.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"363 1","pages":"106 - 97"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82990016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Cloning and Sequence Determination of a gene Encoding an Osmotin-like Protein from Strawberry (Fmgaria X ananassa Duch.) 草莓中渗透蛋白样蛋白基因的克隆与序列测定

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109084473

Junlin Wu, Anwara . Khan, C. Shih, D. S. Shih

引用次数: 16

Cloning and Characterisation of the Hint Homologue of the Thermophile Thermus thermophilus 嗜热菌线索同源基因的克隆与鉴定

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109080772

Yann Gibert, S. Spada, J. Wall, J. Pembroke

引用次数: 0

Isolation of a Member of ets Gene Family in the Polychaete Annelid Perinereis cultrifera 多毛纲环节动物(Perinereis cultrifera) ets基因家族成员的分离

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109047565

B. Bocquet-Muchembled, R. Leroux, Anne Chotteau-LeliÉGvre, F. Fontaine

引用次数: 0

Identification of C15orf5, a Heart-Enriched Transcript on Chromosome 15q23-q24 染色体15q23-q24上心脏富集转录物C15orf5的鉴定

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109042052

L. Carim-Todd, L. Sumoy, N. Andreu, Xavier Estivill And, M. Escarceller

引用次数: 1

Isolation and Characterisation of a cDNA for a Novel Small HSP from Sunflower Suspension Cell Cultures 向日葵悬浮细胞中新型小热休克蛋白cDNA的分离与鉴定

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109084464

A. S. Treglia, M. Gullì, C. Perrotta

引用次数: 2

Characterization of the L. manihotivorans α-Amylase Gene manhotivorans α-淀粉酶基因的鉴定

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109042048

J. Morlon‐Guyot, F. Mucciolo-Roux, R. R. Sanoja, J. Guyot

引用次数: 24

Genomic Structure of Mouse Copper Chaperone, 00×17 小鼠铜伴侣蛋白的基因组结构，00×17

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109084454

Yoshinori Takahashi, K. Kako, K. Ohmura, K. Tsumori, Y. Ohmasa, Shin-ichi Kashiwabara, T. Baba, Eisuke Munekatat

{"title":"Genomic Structure of Mouse Copper Chaperone, 00×17","authors":"Yoshinori Takahashi, K. Kako, K. Ohmura, K. Tsumori, Y. Ohmasa, Shin-ichi Kashiwabara, T. Baba, Eisuke Munekatat","doi":"10.3109/10425170109084454","DOIUrl":"https://doi.org/10.3109/10425170109084454","url":null,"abstract":"Cox17p was first cloned as a cytoplasmic copper chaperone from yeast mutant and recent works suggested the existence of mammalian homologues. Previous report has shown that a gel filtration fraction of heart extract containing porcine Coxl7p peptide promoted the survival of NIH3T3 fibroblast cells. In the present study, we first cloned DNA fragments of the mouse COX17 gene. The mouse COX17 spans ∼6kb and consists of three exons. It was mapped to the center of chromosome 16, using a radiation hybrid-mapping panel. The major transcription start site is 80 bp upstream of the ATG initiation codon as determined by rapid amplification of cDNA ends (5′-RACE) analysis. Two potential polyadenylation sites are 3233 and 3293 bp downstream of the termination codon, respectively. Transient transfection of reporter plasmids containing portions of the mouse COX17 5′-flanking region into AtT-20 and NIH3T3 cells allowed the localization of the essential promoter to a 0.8 kb region upstream of the transcription starting site. Furthermore, the transfected luciferase activity was much higher in AtT-20 than NIH3T3. According to sequence analysis of the –0.8 kb 5′-flanking region, GC rich segments including consensus sequences for binding of the transcription factor Sp1, but no The TATAICAAT boxes, exist in the region of the transcription start site. Besides the GC box, binding sites for NRF-1 and 2 known as specific transcription factors for COX subunits are also localized around the transcription starting site.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"19 1","pages":"305 - 318"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85097925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Nucleotide Sequence of Envelope Protein of Japanese Encephalitis Virus SA14-14-2 Adapted to Vero Cells 日本脑炎病毒SA14-14-2包膜蛋白的核苷酸序列

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109084471

S. Hong, W. Yoo, R. Putnak, K. Eckels, H. Rho, Soo‐Ok Kim

{"title":"Nucleotide Sequence of Envelope Protein of Japanese Encephalitis Virus SA14-14-2 Adapted to Vero Cells","authors":"S. Hong, W. Yoo, R. Putnak, K. Eckels, H. Rho, Soo‐Ok Kim","doi":"10.3109/10425170109084471","DOIUrl":"https://doi.org/10.3109/10425170109084471","url":null,"abstract":"Live attenuated Japanese encephalitis (JE) virus SA14-14-2 produced in primary dog kidney cells (PDK) was adapted to Vero cells. In an effort to gain insight into the molecular basis of the biological characteristics of the SA14-14-2(Vero) strain, the 1500 nucleotide sequence encoding the envelope (E) gene which possesses major neutralizing epitopes was determined and compared with the sequences of two other attenuated JE virus strains, SA14-14-2(PHK) and SA14-14-2(PDK). The amino acid sequence of the C-terminal region (a.a. 280-500) was found to be identical for all three strains, while the N-terminal region (a.a. 1-279) shows sequence variation. The distribution of mutations in the N-terminal region was nearly the same among the three attenuated strains, suggesting that the N-terminal sequences might be related with virus-host cell specificity. However, it was found that Lys and Val (a.a. 138 and 176, respectively), known to be responsible for attenuation, are still conserved in SA14-14-2(Vero). Animal testing showed that SA14-14-2(Vero) has an attenuation phenotype similar to that of the parent s SA14-14-2(PDK) strain in mice.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"17 1","pages":"437 - 442"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82640256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5