Isolation and Characterisation of a cDNA for a Novel Small HSP from Sunflower Suspension Cell Cultures

A. S. Treglia, M. Gullì, C. Perrotta
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引用次数: 2

Abstract

A full-length cDNA encoding a novel small heat shock protein (HaHSP17.9) was isolated from a cDNA library of sunflower (Helianthus annuus cv. Gloriasol). The deduced amino acid sequence exhibited high degree homology to the class I cytosolic sHSPs from other plant species, and contained all the conserved regions characteristic of this class of proteins. Northern analyses showed that the transcript homologous to HaHSP17.9 accumulates during heat shock in suspension cultured cells and in the different parts of sunflower seedlings.
向日葵悬浮细胞中新型小热休克蛋白cDNA的分离与鉴定
从向日葵(Helianthus annuus cv.) cDNA文库中分离到一个新的小热休克蛋白(HaHSP17.9)的全长cDNA。Gloriasol)。推导出的氨基酸序列与其他植物的I类胞质sHSPs具有高度的同源性,并包含该类蛋白的所有保守区域。Northern分析表明,HaHSP17.9同源转录本在悬浮培养细胞和向日葵幼苗不同部位的热休克过程中积累。
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