Dose-Response最新文献_第9页

Verification of Preferred Reference Genes for RT-qPCR Analysis of Radiosensitivity Gene in Human Peripheral Blood. 人外周血放射敏感基因RT-qPCR分析优选内参基因的验证。

IF 2.4 4区医学

Dose-Response Pub Date : 2025-10-15 eCollection Date: 2025-10-01 DOI: 10.1177/15593258251384827

Mingquan He, Xueqin Yang, Yuting Chen, Chaoxian Gao

{"title":"Verification of Preferred Reference Genes for RT-qPCR Analysis of Radiosensitivity Gene in Human Peripheral Blood.","authors":"Mingquan He, Xueqin Yang, Yuting Chen, Chaoxian Gao","doi":"10.1177/15593258251384827","DOIUrl":"10.1177/15593258251384827","url":null,"abstract":"Objective: High-throughput gene expression analysis represents a promising approach for evaluating irradiation doses in vitro. This study aimed to identify preferred reference genes for normalizing RT-qPCR data in human peripheral blood following X-ray irradiation.Methods: We assessed the stability of GAPDH, 18S rRNA, UBC, HPRT, DPM1, ITFG1, MRPS5, and ACTB, which are commonly utilized in radiation biodosimetry studies. Three whole blood samples from healthy donors were divided into three culture time groups (2, 12, and 24 hours) and subjected to X-ray doses of 0, 0.5, 1.2, and 3.5 Gy, resulting in a total of 12 subgroups. NormFinder, geNorm, BestKeeper, and ΔCt were employed to evaluate the stability of the candidate genes. RefFinder was utilized to comprehensively rank the candidates and identify suitable reference genes.Results: The preferred reference genes identified were UBC, HPRT, and GAPDH for 2-hour culture time; for 12 hours, UBC, HPRT, and 18S rRNA were preferred; and for 24 hours, 18S rRNA, MRPS5, and GAPDH were preferred.Conclusion: This study presents novel findings of reference genes suitable for normalizing RT-qPCR data following X-ray irradiation in human peripheral blood across three distinct culture periods. It offers valuable insights for the selection of reference genes in radiation biodosimetry research.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":"23 4","pages":"15593258251384827"},"PeriodicalIF":2.4,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12536175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145344145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptive Responses in High-Radiation Environments: Insights From Chernobyl Wildlife and Ramsar Residents. 高辐射环境下的适应性反应：来自切尔诺贝利野生动物和拉姆萨尔居民的见解。

IF 2.4 4区医学

Dose-Response Pub Date : 2025-10-15 eCollection Date: 2025-10-01 DOI: 10.1177/15593258251385632

Smj Mortazavi, Sahel Rabiee, AmirAli Fallah, Razieh Rashidfar, Zeynab Seyyedi, Hassan Vafapour, Alireza Mortazavi, Fatemeh Makarempour, Zahra Kherad, Marzieh Abdollahi, James S Welsh

{"title":"Adaptive Responses in High-Radiation Environments: Insights From Chernobyl Wildlife and Ramsar Residents.","authors":"Smj Mortazavi, Sahel Rabiee, AmirAli Fallah, Razieh Rashidfar, Zeynab Seyyedi, Hassan Vafapour, Alireza Mortazavi, Fatemeh Makarempour, Zahra Kherad, Marzieh Abdollahi, James S Welsh","doi":"10.1177/15593258251385632","DOIUrl":"10.1177/15593258251385632","url":null,"abstract":"This mini-review explores adaptive responses in organisms exposed to high radiation levels, drawing comparisons between Chernobyl's wildlife-specifically its darker-pigmented frogs-and residents of Ramsar, Iran, a region with high natural background radiation. Chernobyl's wildlife adaptations are not surprising, as substantial evidence in humans, demonstrates similar adaptation to high radiation levels. Studies reveal that mechanisms such as increased melanin production in frogs and enhanced DNA repair capabilities in Ramsar residents help mitigate radiation damage. These adaptations provide a framework for understanding resilience to environmental stressors and contribute to broader discussions on evolutionary survival mechanisms in extreme environments. By examining ecological and physiological responses across species, this review sheds light on radiation's role in natural selection and potential applications for environmental and radiobiological research.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":"23 4","pages":"15593258251385632"},"PeriodicalIF":2.4,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12536131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145344168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lycium barbarum Polysaccharides Suppress Radiation-Induced Microglial Inflammation by Inhibiting the IKKβ/IκBα/NF-κB Pathway. 枸杞多糖通过抑制IKKβ/ i -κB α/NF-κB通路抑制辐射诱导的小胶质细胞炎症。

IF 2.4 4区医学

Dose-Response Pub Date : 2025-10-04 eCollection Date: 2025-10-01 DOI: 10.1177/15593258251382964

Xing Zhang, Qun Liu, Lian Liu, Feng Qian, Bo Xu Ren, Feng Ru Tang

{"title":"Lycium barbarum Polysaccharides Suppress Radiation-Induced Microglial Inflammation by Inhibiting the IKKβ/IκBα/NF-κB Pathway.","authors":"Xing Zhang, Qun Liu, Lian Liu, Feng Qian, Bo Xu Ren, Feng Ru Tang","doi":"10.1177/15593258251382964","DOIUrl":"10.1177/15593258251382964","url":null,"abstract":"Background: Radiation therapy for brain tumors often leads to radiation-induced brain injury, which is closely linked to microglial hyperactivation and neuroinflammation. Lycium barbarum polysaccharide (LBP), the primary active component of Lycium barbarum, may provide neuroprotection by suppressing microglial overactivation and reducing neuroinflammation.Methods: BV2 microglial cells were pretreated with LBP for 12 hours (h), exposed to 10 Gy X-ray irradiation, and then post-treated with LBP for another 12 h. We assessed microglial polarization and measured levels of nitric oxide (NO), interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and key proteins in the IKKβ/IκBα/NF-κB pathway.Results: LBP treatment shifted microglia from the pro-inflammatory M1 phenotype to the anti-inflammatory M2 phenotype and significantly decreased the release of NO, IL-1β, and TNF-α following irradiation.Conclusion: Our findings demonstrate that LBP mitigates radiation-induced microglial inflammation by inhibiting the IKKβ/IκBα/NF-κB pathway, suggesting its potential as a radioprotective agent against radiotherapy-induced neuroinflammation.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":"23 4","pages":"15593258251382964"},"PeriodicalIF":2.4,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12496469/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacological Intervention of Post-traumatic Seizure: Advanced Research Progress. 外伤性癫痫发作的药物干预研究进展

IF 2.4 4区医学

Dose-Response Pub Date : 2025-09-29 eCollection Date: 2025-07-01 DOI: 10.1177/15593258251384796

Weiwei Zeng, Juanfeng Ou, Ruitong Li, Yong Yin, Xiaoying Lin, Yingting Lu, Mimi Tang

{"title":"Pharmacological Intervention of Post-traumatic Seizure: Advanced Research Progress.","authors":"Weiwei Zeng, Juanfeng Ou, Ruitong Li, Yong Yin, Xiaoying Lin, Yingting Lu, Mimi Tang","doi":"10.1177/15593258251384796","DOIUrl":"10.1177/15593258251384796","url":null,"abstract":"Traumatic brain injury (TBI) is an important condition with high rates of disability and mortality worldwide. Post-traumatic seizure (PTS) frequently occur following TBI, manifesting in both early and late stages. Recurrent PTS without timely intervention may progress to post-traumatic epilepsy (PTE), which defined as the occurrence of two or more unprovoked seizures. Early pharmacological intervention is essential to mitigate the risk of PTE and enhance the prognosis for patients with TBI. Antiepileptic drugs (AEDs) offer a viable strategy for managing PTS. Recent studies indicated that AEDs are more effective in early post-traumatic seizure compared to late post-traumatic seizure, and their efficacy and safety require further evaluation. As research advances in the pathophysiological changes after TBI and the pathogenesis of PTS, current investigations are increasingly focused on neurological damage. Novel compounds targeting various pathways, including antioxidants, anti-neuroinflammatory agents, glutamate modulators and anti-oxidative stress compounds, have demonstrated promising potential in preclinical studies for PTS intervention. This review focuses on the research progress of different AEDs in PTS intervention and discusses the recent developments of emerging PTS intervention strategies based on multiple pathways, providing insights into the clinical application of AEDs and new directions for the development of new drugs for PTS intervention.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":"23 3","pages":"15593258251384796"},"PeriodicalIF":2.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12480794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

INWORKS Solid Cancer Mortality Risk Estimate is Much Higher than the Equivalent LSS Risk Estimate. INWORKS实体癌死亡率风险估计远高于等效的LSS风险估计。

IF 2.4 4区医学

Dose-Response Pub Date : 2025-09-22 eCollection Date: 2025-07-01 DOI: 10.1177/15593258251382312

Ken Chaplin

引用次数: 0

Retraction: Dose-Dependent, Antidepressant, and Anxiolytic Effects of a Traditional Medicinal Plant for the Management of Behavioral Dysfunctions in Animal Models. 撤回：一种传统药用植物对动物模型中行为功能障碍的剂量依赖、抗抑郁和抗焦虑作用。

IF 2.4 4区医学

Dose-Response Pub Date : 2025-09-20 eCollection Date: 2025-07-01 DOI: 10.1177/15593258251379150

引用次数: 0

Toosendanin Attenuates Mycoplasma pneumonia Induced Pneumonia (MPP) in Mice via Inhibiting NF-κB-Mediated Inflammatory Response: In vivo and silico Studies. 仙丹素通过抑制NF-κ b介导的炎症反应减轻小鼠肺炎支原体诱导的肺炎（MPP）：体内和体外研究。

IF 2.4 4区医学

Dose-Response Pub Date : 2025-09-18 eCollection Date: 2025-07-01 DOI: 10.1177/15593258251367619

Yin Zhou, Xinyou Su, Cuicui Wang, Abdullah A Alarfaj, Abdurahman Hajinur Hirad

{"title":"Toosendanin Attenuates Mycoplasma pneumonia Induced Pneumonia (MPP) in Mice via Inhibiting NF-κB-Mediated Inflammatory Response: In vivo and silico Studies.","authors":"Yin Zhou, Xinyou Su, Cuicui Wang, Abdullah A Alarfaj, Abdurahman Hajinur Hirad","doi":"10.1177/15593258251367619","DOIUrl":"10.1177/15593258251367619","url":null,"abstract":"Background: In the present study, the therapeutic effects of Toosendanin (TSN) against Mycoplasma pneumoniae (MP)-induced pneumonia (MPP) in mice.Research design: Swiss albino mice were exposed to MP culture for 2 days, causing pneumonia, and then treated with TSN for 3 days. Lung weights, total protein, IgM, C-reactive protein, oxidative stress, and inflammatory cytokines were assessed. Histological alterations were evaluated in lung tissues. Molecular docking analysis was performed to test TSN's interaction with inflammatory cytokines, including interleukin-1 beta (IL-1β), interleukin-6 (IL-6), transforming growth factor-beta 1 (TGF-β1), and nuclear factor kappa B (NF-κB) were evaluated targets.Results: TSN treatment significantly reduced lung weight by approximately 25% compared to the MP-infected group (P < 0.05). Total protein and C-reactive protein (CRP) levels decreased by 30% and 40%, respectively. Malondialdehyde (MDA), was reduced by 35%, while antioxidant enzyme levels (e.g., SOD, CAT) increased by 20%-25%. Pro-inflammatory cytokines such as IL-1β and IL-6 were significantly lowered by 40%-50%. Histological analysis revealed a marked reduction in inflammatory cell infiltration and alveolar damage scores (P < 0.01). Molecular docking confirmed strong binding interactions between IL-1β, IL-6, TGF-β1, NF-κB and TSN.Conclusions: The present findings confirm the beneficial effects of TSN in protecting mice from pneumonia.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":"23 3","pages":"15593258251367619"},"PeriodicalIF":2.4,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12446804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145112223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of Radiotherapy FLASH-RT: From Treating Lung Cancer to Preventing Pulmonary Fibrosis. 放疗FLASH-RT的疗效：从治疗肺癌到预防肺纤维化。

IF 2.4 4区医学

Dose-Response Pub Date : 2025-09-15 eCollection Date: 2025-07-01 DOI: 10.1177/15593258251379921

Tianyu Yang, Zhiming Xu, Wentao Hu, Zhifei Cao, Yongsheng Zhang

引用次数: 0

Unveiling Nephroprotective, Diuretic and Toxicity Response of Pinus roxburghii Sarg. Extract by In-vitro, In-vivo and Docking Techniques. 刺梨松的肾保护、利尿和毒性反应。体外、体内和对接提取技术。

IF 2.4 4区医学

Dose-Response Pub Date : 2025-09-08 eCollection Date: 2025-07-01 DOI: 10.1177/15593258251352705

Maddiha Nasir, Fatima Saqib, Muhammad Farhaj Latif, Codrut Loan Ciurea, Catalin Misarca, Marius Alexandru Moga, Oana Andreescu

{"title":"Unveiling Nephroprotective, Diuretic and Toxicity Response of Pinus roxburghii Sarg. Extract by In-vitro, In-vivo and Docking Techniques.","authors":"Maddiha Nasir, Fatima Saqib, Muhammad Farhaj Latif, Codrut Loan Ciurea, Catalin Misarca, Marius Alexandru Moga, Oana Andreescu","doi":"10.1177/15593258251352705","DOIUrl":"10.1177/15593258251352705","url":null,"abstract":"Objective: Pinus roxburghii Sarg. (Pinaceae) has shown useful effects in the treatment of urinary problems. This research aimed to explore P.roxburghii scientifically via in-vitro, in-vivo and in-silico techniques to find out its therapeutic benefits on kidney and bladder.Method: Pharmacologically active components of aqueous ethanolic extract of P.roxburghii were analyzed by HPLC-DAD screening. These components were predicted for multfi-mechanisms and spasmolytic effects in network pharmacology. In-vitro experiments were conducted on isolated bladder of rat to analyze the spasmolytic effects of plant extract. Spasmolytic effects were also compared with the standard drugs verapamil and oxybutynin In-vivo experiments were also performed to observe diuretic and nephroprotective potential of the plant.Result: Phytochemical analysis indicated the presence of chlorogenic acid, gallic acid, P-coumaric acid, kaempferol, salicylic acid, sinapic acid and benzoic acid. Aqueous ethanolic extract of plant relaxed the contractions induced by high K+ (80 mm) and carbachol (1 µM) in isolated urinary bladder strips of rat. In-vivo experiments revealed the nephroprotective and diuretic potential.Conclusion: So it has been proved via scientific evidence that the concerned plant possesses spasmolytic, nephroprotective and diuretic potential and may be an important therapeutic agent against urinary problems in the near future.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":"23 3","pages":"15593258251352705"},"PeriodicalIF":2.4,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12421008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145039393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Questioning the Linear No-Threshold Model (LNT): Lessons From Hiroshima/Nagasaki and Fukushima. 质疑线性无阈模型（LNT）：广岛/长崎和福岛的教训。

IF 2.4 4区医学

Dose-Response Pub Date : 2025-09-06 eCollection Date: 2025-07-01 DOI: 10.1177/15593258251367588

Shizuyo Sutou

{"title":"Questioning the Linear No-Threshold Model (LNT): Lessons From Hiroshima/Nagasaki and Fukushima.","authors":"Shizuyo Sutou","doi":"10.1177/15593258251367588","DOIUrl":"10.1177/15593258251367588","url":null,"abstract":"Living organisms have been exposed to ionizing radiation throughout Earth's 4-billion-year history, with humans presently receiving about 2 mSv of ionizing radiation every year. While radiation generates reactive oxygen and nitrogen species (ROS and RNS), organisms have evolved mechanisms to neutralize these toxic molecules and utilize them as signal transducers. High doses of radiation are harmful, but low doses are seemingly essential, and moderate doses can provide benefits-a phenomenon known as hormesis. Radiation exposure is currently regulated by the linear no-threshold model (LNT), which assumes all radiation is harmful, even at the smallest doses. However, substantial evidence, including insights into biological defense mechanisms like DNA repair, apoptosis, and immune system, supports hormesis. Although the Life Span Study (LSS) data historically backed the LNT, closer analysis reveals that low-dose radiation is linked to increased life expectancy and reduced cancer risk, invalidating LNT. During the 2011 Fukushima nuclear disaster, the Japanese government, adhering to the LNT-based precautionary principle, evacuated residents despite low contamination levels. This decision caused over 2000 deaths, though no fatalities were directly attributed to radiation. These findings challenge the LNT model and highlight the need for regulatory standards that incorporate thresholds and/or hormesis principles, better reflecting biological evidence.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":"23 3","pages":"15593258251367588"},"PeriodicalIF":2.4,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12414125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0