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New immunoassay systems based on recombinant human lactoferrin 基于重组人乳铁蛋白的新型免疫测定系统
Doklady of the National Academy of Sciences of Belarus Pub Date : 2021-07-16 DOI: 10.29235/1561-8323-2021-65-3-290-302
D. A. Semenov, I. Vashkevich, O. Sviridov
{"title":"New immunoassay systems based on recombinant human lactoferrin","authors":"D. A. Semenov, I. Vashkevich, O. Sviridov","doi":"10.29235/1561-8323-2021-65-3-290-302","DOIUrl":"https://doi.org/10.29235/1561-8323-2021-65-3-290-302","url":null,"abstract":"In this work, soluble and solid phase immunoreagents, including recombinant human lactoferrin (rhLF), a complex of rhLF with europium ions, rabbit antiserum to rhLF, anti-rhLF immunoglobulin purified by antigen-affinity chromatography and the conjugates of this immunoglobulin with an Eu3+ chelate or horseradish peroxidase have been obtained by a combination of biochemical and synthetic methods using rhLF as an initial compound. Biospecific interactions of the reagents in four immunochemical systems were assessed by measuring the enzyme activity or time-resolved fluorescence. The study resulted in the development of fast and precise immunoassays for biologically active rhLF in transgenic goat milk and in protein fractions obtained in the course of pure rhLF manufacture, as well as in pharmaceutical preparations and food additives.","PeriodicalId":11283,"journal":{"name":"Doklady of the National Academy of Sciences of Belarus","volume":"108 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74138743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Multilevel algorithm for color clustering of images 图像颜色聚类的多级算法
Doklady of the National Academy of Sciences of Belarus Pub Date : 2021-07-16 DOI: 10.29235/1561-8323-2021-65-3-269-274
B. Zalesky
{"title":"Multilevel algorithm for color clustering of images","authors":"B. Zalesky","doi":"10.29235/1561-8323-2021-65-3-269-274","DOIUrl":"https://doi.org/10.29235/1561-8323-2021-65-3-269-274","url":null,"abstract":"The fast multilevel algorithm to cluster color images (MACC – Multilevel Algorithm for Color Clustering) is presented. Currently, several well-known algorithms of image clustering, including the k‑means algorithm (which is one of the most commonly used in data mining) and its fuzzy versions, watershed, region growing ones, as well as a number of new more complex neural network and other algorithms are actively used for image processing. However, they cannot be applied for clustering large color images in real time. Fast clustering is required, for example, to process frames of video streams shot by various video cameras or when working with large image databases. The developed algorithm MACC allows the clustering of large images, for example, FullHD size, on a personal computer with an average deviation from the original color values of about five units in less than 20 milliseconds, while a parallel version of the classical k‑means algorithm performs the clustering of the same images with an average error of more than 12 units for a time exceeding 2 seconds. The proposed algorithm of multilevel color clustering of images is quite simple to implement. It has been extensively tested on a large number of color images.","PeriodicalId":11283,"journal":{"name":"Doklady of the National Academy of Sciences of Belarus","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89647059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of the differential gene expression in anthracnose treated seedlings of yellow lupin 黄豆炭疽病处理幼苗差异基因表达的评价
Doklady of the National Academy of Sciences of Belarus Pub Date : 2021-07-16 DOI: 10.29235/1561-8323-2021-65-3-330-336
E. Sysoliatin, V. Anokhina, N. Anisimova, O. Babak, A. Kilchevsky
{"title":"Assessment of the differential gene expression in anthracnose treated seedlings of yellow lupin","authors":"E. Sysoliatin, V. Anokhina, N. Anisimova, O. Babak, A. Kilchevsky","doi":"10.29235/1561-8323-2021-65-3-330-336","DOIUrl":"https://doi.org/10.29235/1561-8323-2021-65-3-330-336","url":null,"abstract":"Seedlings of yellow lupine treated with Colletotrichum lupini isolate were studied by the method of SRAP-analysis with the purpose to assess the differential expression of genes. As a result, the PCR fragment corresponding to tolerant seedlings was found. The genetic determinants found are likely involved in the control of the resistance (tolerance) of lupine plants to anthracnose.","PeriodicalId":11283,"journal":{"name":"Doklady of the National Academy of Sciences of Belarus","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78817626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
5 Belarus Begins 白俄罗斯开始
Doklady of the National Academy of Sciences of Belarus Pub Date : 2021-05-04 DOI: 10.12987/9780300260878-008
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引用次数: 0
Conclusions: Lukashenka’s Rise and Fall 结论:卢卡申科的兴衰
Doklady of the National Academy of Sciences of Belarus Pub Date : 2021-05-04 DOI: 10.12987/9780300260878-018
{"title":"Conclusions: Lukashenka’s Rise and Fall","authors":"","doi":"10.12987/9780300260878-018","DOIUrl":"https://doi.org/10.12987/9780300260878-018","url":null,"abstract":"","PeriodicalId":11283,"journal":{"name":"Doklady of the National Academy of Sciences of Belarus","volume":"113 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89043833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
10 Building an Authoritarian State: Lukashenka’s Second Term 建立专制国家:卢卡申科的第二任期
Doklady of the National Academy of Sciences of Belarus Pub Date : 2021-05-04 DOI: 10.12987/9780300260878-013
{"title":"10 Building an Authoritarian State: Lukashenka’s Second Term","authors":"","doi":"10.12987/9780300260878-013","DOIUrl":"https://doi.org/10.12987/9780300260878-013","url":null,"abstract":"","PeriodicalId":11283,"journal":{"name":"Doklady of the National Academy of Sciences of Belarus","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86663961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
11 The Third Term: The Edifice Crumbles 第十一章第三期:大厦崩塌
Doklady of the National Academy of Sciences of Belarus Pub Date : 2021-05-04 DOI: 10.12987/9780300260878-014
{"title":"11 The Third Term: The Edifice Crumbles","authors":"","doi":"10.12987/9780300260878-014","DOIUrl":"https://doi.org/10.12987/9780300260878-014","url":null,"abstract":"","PeriodicalId":11283,"journal":{"name":"Doklady of the National Academy of Sciences of Belarus","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74996473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
6 Belarus Begins Again: The Traumatic Twentieth Century 白俄罗斯的重新开始:充满创伤的二十世纪
Doklady of the National Academy of Sciences of Belarus Pub Date : 2021-05-04 DOI: 10.12987/9780300260878-009
{"title":"6 Belarus Begins Again: The Traumatic Twentieth Century","authors":"","doi":"10.12987/9780300260878-009","DOIUrl":"https://doi.org/10.12987/9780300260878-009","url":null,"abstract":"","PeriodicalId":11283,"journal":{"name":"Doklady of the National Academy of Sciences of Belarus","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73028103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
7 The Building Blocks of National Identity 国家认同的构成要素
Doklady of the National Academy of Sciences of Belarus Pub Date : 2021-05-04 DOI: 10.12987/9780300260878-010
{"title":"7 The Building Blocks of National Identity","authors":"","doi":"10.12987/9780300260878-010","DOIUrl":"https://doi.org/10.12987/9780300260878-010","url":null,"abstract":"","PeriodicalId":11283,"journal":{"name":"Doklady of the National Academy of Sciences of Belarus","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81269359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
2 Litva
Doklady of the National Academy of Sciences of Belarus Pub Date : 2021-05-04 DOI: 10.12987/9780300260878-005
{"title":"2 Litva","authors":"","doi":"10.12987/9780300260878-005","DOIUrl":"https://doi.org/10.12987/9780300260878-005","url":null,"abstract":"","PeriodicalId":11283,"journal":{"name":"Doklady of the National Academy of Sciences of Belarus","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83624478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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