Drug Development and Industrial Pharmacy最新文献

{"title":"Exploration of synergistically engineered invasomes of fluconazole incorporated with safranal against onychomycosis for enhanced transungual delivery.","authors":"Syeda Nashvia Adin, Isha Gupta, Mohd Aqil, Mohd Mujeeb","doi":"10.1080/03639045.2024.2437050","DOIUrl":"10.1080/03639045.2024.2437050","url":null,"abstract":"<p><strong>Objective: </strong>The preparation of safranal-containing invasomes for fluconazole (FLU-IN) is investigated in the current research work to augment FLU permeation, bioavailability, and solubility <i>via</i> nail for transungual delivery.</p><p><strong>Methods: </strong>FLU-IN was prepared utilizing the 'thin-film hydration process', and for optimization, 'Box-Behnken design (BBD)' was employed. Entrapment efficiency (EE), Poly-dispersity index (PDI), <i>in vitro</i> FLU release, vesicle size and zeta potential were used to characterize FLU-INopt. Confocal microscopy (CLSM), nail permeation investigation, and Transmission electron microscopy (TEM) were also carried out for further examination.</p><p><strong>Results: </strong>The FLU-INopt demonstrated tiny, spherical, sealed-shape vesicles with a vesicle size of 140.3 nm, PDI of 0.1604, <i>in vitro</i> release of 84.32%, and entrapment efficiency of 74.65%. According to the CLSM investigation, the prepared formulation exhibits better FLU penetration over the nail than FLU suspension gel. Compared to the standard fluconazole marketed gel, the anti-fungal investigation showed that the FLU-IN gel has good anti-fungal potential against <i>Trichophyton rubrum, Nakaseomyces glabrata and Candida albicans</i>. Additional research on Wistar albino rats' skin irritancy supports the new FLU-IN formulation's safety for topical treatment.</p><p><strong>Conclusion: </strong>The present study supported the claim that the developed invasomal formulation is a desirable vesicular carrier for FLU transungual delivery for the management of onychomycosis.</p>","PeriodicalId":11263,"journal":{"name":"Drug Development and Industrial Pharmacy","volume":" ","pages":"1031-1043"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitooligosaccharide-modified PLGA-loaded PPD nanoparticles ameliorated sepsis-associated acute kidney injury <i>via</i> the NF-κB signaling pathway.","authors":"Baifang Gong, Yawen Yu, Xinxin Bai, Yaping He, Tao Pan, Teng Liu, Zhixia Wang, Ke Liu, Huaying Fan","doi":"10.1080/03639045.2024.2434958","DOIUrl":"10.1080/03639045.2024.2434958","url":null,"abstract":"<p><strong>Objectives: </strong>Sepsis-associated acute kidney injury (SA-AKI) is a significant clinical challenge with high morbidity and mortality. Low bioavailability of protopanaxadiol (PPD) limits its clinical application. In this study, PPD was encapsulated with chitooligosaccharide (COS) modified polylactic-co-glycolic acid (PLGA) to develop novel nanomedicines for the treatment of SA-AKI.</p><p><strong>Methods: </strong>COS-PLGA-PPD nanoparticles were prepared by emulsified solvent evaporation method, and their properties were evaluated. <i>In vitro</i>, the anti-inflammatory and protective effects of COS-PLGA-PPD NPs were investigated in a cellular model of LPS-induced NRK-52E cells and their uptake in Caco-2 cells. Indicators of renal injury, inflammation, and NF-κB signaling pathway were evaluated by injecting LPS into SD rats and inducing SA-AKI model <i>in vivo</i>. The oral bioavailability of nanoparticles was evaluated by pharmacokinetics.</p><p><strong>Results: </strong>Compared with PPD and unmodified nanoparticles, COS-PLGA-PPD NPs were more stable, with a particle size of 139.69 nm, which enhanced the viability of NRK-52E cells, increased the uptake of Caco-2 cells, alleviated the symptoms of SA-AKI in rats, inhibited the NF-κB signaling pathway, reduced the expression of inflammatory factors, and had a bioavailability 1.7-fold that of PPD.</p><p><strong>Conclusion: </strong>COS-PLGA-PPD NPs ameliorate LPS-induced SA-AKI in rats by inhibiting the NF-κB signaling pathway, providing a basis for the treatment of SA-AKI.</p>","PeriodicalId":11263,"journal":{"name":"Drug Development and Industrial Pharmacy","volume":" ","pages":"1008-1020"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of processing parameters on characteristics of biodegradable extended-release microspheres containing leuprolide acetate.","authors":"Ngo Giao Thong, Bui Thi Ngoc Ha, Bui Thi Thuong, Nguyen Thanh Hai, Thi Hai Yen Tran","doi":"10.1080/03639045.2024.2433621","DOIUrl":"10.1080/03639045.2024.2433621","url":null,"abstract":"<p><strong>Objective: </strong>Poly(lactic-<i>co</i>-glycolic acid) microsphere containing leuprolide acetate - an extended-release drug delivery system whose characteristics (i.e. loading capacity, particle size and initial burst phase) depend on processing parameters.</p><p><strong>Methods: </strong>Microspheres were prepared by water/oil/water double-emulsion solvent evaporation method; drug content in microspheres was determined by high-performance liquid chromatography (HPLC); peptide concentration in the release medium was measured by fluorescence spectrometer; particle size and particle size distribution were measured by laser diffraction method; interaction between poly(lactic-<i>co</i>-glycolic acid) (PLGA) and leuprolide acetate (LA) was determined by differential scanning calorimetry (DSC) and Fourier-transform infrared spectroscopy (FTIR).</p><p><strong>Results: </strong>DSC curves and assay results proved LA adsorption ability of PLGA film. FTIR spectra proved ionic interactions between positive charged LA molecules and negative charged PLGA chains in phosphate buffer pH 7.4. Ten processing parameters including LA concentration (mg/mL), PLGA concentration (mg/mL), W1/O ratio (v/v), the first homogenization time (min), the first homogenization speed (rpm), O/W2 ratio (v/v), PVA concentration of W2 phase (mg/ml), the second homogenization time (s), the volume of diluted solution (ml) and nitrogen aeration time (min) have impacts on loading capacity, particle size and initial burst phase of microspheres. The release exponent (n) of Korsmeyer-Peppas model was 0.3571 (lower than 0.43), indicating that Fickian diffusion manipulated release kinetics of initial burst phase.</p><p><strong>Conclusions: </strong>Processing parameter modification contributes to small microspheres with high loading capacity and controlled initial burst phase.</p>","PeriodicalId":11263,"journal":{"name":"Drug Development and Industrial Pharmacy","volume":" ","pages":"981-994"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing critical quality attributes of fast disintegrating tablets using artificial neural networks: a scientific benchmark study.","authors":"Jagruti Desai, Prince Dhameliya, Swayamprakash Patel","doi":"10.1080/03639045.2024.2434640","DOIUrl":"10.1080/03639045.2024.2434640","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study is to create predictive models utilizing machine learning algorithms, including Artificial Neural Networks (ANN), k-nearest neighbor (kNN), support vector machines (SVM), and linear regression, to predict critical quality attributes (CQAs) such as hardness, friability, and disintegration time of fast disintegrating tablets (FDTs).</p><p><strong>Methods: </strong>A dataset of 864 batches of FDTs was generated by varying binder types and amounts, disintegrants, diluents, punch sizes, and compression forces. Preprocessing steps included normalizing numerical features based on industry standards, one-hot encoding for categorical variables, and addressing outliers to ensure data consistency. Four machine learning models were trained and evaluated on R² values and mean squared error (MSE). Feature importance was analyzed using permutation importance, and statistical validation (<i>p</i> < 0.05) and confidence intervals were computed for model performance. The 'differential_evolution' function was used to optimize the formulation.</p><p><strong>Results: </strong>Among the models, ANN demonstrated the highest predictive accuracy, achieving R² values up to 0.9550 with the lowest MSE across training and test datasets, outperforming kNN, SVM, and linear regression. The ANN's ability to model complex, non-linear interactions between formulation variables was statistically significant, as validated through six checkpoint batches of acetylsalicylic acid FDTs. The feature importance analysis revealed compression force, binder type, and punch size as the most influential factors affecting hardness, while disintegrant type influenced friability. The 'differential_evolution' function effectively optimized the CQAs, resulting in FDTs with ideal characteristics.</p><p><strong>Conclusion: </strong>The ANN model, integrated with differential evolution, provided a robust tool for optimizing FDT formulations by accurately predicting CQAs and reducing the need for extensive experimental trials. Compared to traditional optimization methods, ANN excels in capturing intricate multi-variable relationships, making it a valuable approach for scaling beyond acetylsalicylic acid to other formulations. This method enhances the consistency and efficiency of tablet formulation, supporting broader pharmaceutical applications.</p>","PeriodicalId":11263,"journal":{"name":"Drug Development and Industrial Pharmacy","volume":" ","pages":"995-1007"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liposomal delivery of <i>Annona muricata</i> leaves extract for the treatment of hepatocellular carcinoma.","authors":"Rituraj Bharadwaj, Achyut Bora, Kangkana Sharma","doi":"10.1080/03639045.2024.2433618","DOIUrl":"10.1080/03639045.2024.2433618","url":null,"abstract":"<p><strong>Background: </strong>Liver in the body plays vital role including digestion, detoxification, metabolism and even production of hormones. Hepatocellular carcinoma is recognized as one of leading cause of death worldwide. Infection with hepatitis B and C virus, nonalcoholic fatty liver disease and excessive consumption of alcohol are among the most common risk factors associated with the development of hepatocellular carcinoma.</p><p><strong>Objective: </strong>The present research study involves formulation of liposomal delivery of methanolic extract of <i>Annona muricata</i> as an alternative for the treatment of hepatocellular carcinoma.</p><p><strong>Methods: </strong>The methanolic extract of <i>Annona muricata</i> was subjected for both nonvolatile and volatile content analysis by performing phytochemical screening and GCMS. The methanolic extract was entrapped within the liposomes for its effective delivery. The prepared liposomes were characterized in-vitro, and the optimized formulation was further evaluated against hepatocellular carcinoma induced in the animal model.</p><p><strong>Results: </strong>The methanolic extract showed the presence of alkaloid, carbohydrate, flavonoid, tannin, proteins and acetogenins, whereas the GMCS analysis depicts presence of 12 different compounds. The optimized in-vitro analysis of prepared liposomes showed a particle size of 107.2 ± 1.7 nm, zeta potential of -30.6 mV and entrapment efficiency of 62.15%. TEM micrograph of the optimized liposome formulation has showed spherical geometry with homogenous distribution and negligible agglomeration. In-vivo anticancer study reveals the potent efficacy of the formulation for the treatment of hepatocellular carcinoma.</p><p><strong>Conclusion: </strong>The research findings have established the efficacy of the methanolic extract of <i>Annona muri</i>cata in the treatment of hepatocellular carcinoma.</p>","PeriodicalId":11263,"journal":{"name":"Drug Development and Industrial Pharmacy","volume":" ","pages":"968-980"},"PeriodicalIF":2.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyroligneous extract, a biomaterial derived from pyrolytic palm kernel shell wood vinegar, as a novel diabetic wound healing aid: an animal study.","authors":"Yongyuth Theapparat, Sunisa Khongthong, Natthrit Roekngam, Tan Suwandecha, Jongdee Nopparat, Damrongsak Faroongsarng","doi":"10.1080/03639045.2024.2427795","DOIUrl":"10.1080/03639045.2024.2427795","url":null,"abstract":"<p><strong>Objective: </strong>Wound in diabetes is difficult to heal since it possesses excessive inflammation. The aim of the study was to evaluate wound healing activity of chitosan-based hydrogel containing pyroligneous acid in diabetic animals.</p><p><strong>Significance: </strong>Pyroligneous acid, a byproduct of biochar production from palm kernel shell biomass, contained oxygenated compounds which, with extracting enrichment, could promote wound healing.</p><p><strong>Methods: </strong>Streptozotocin-induced diabetic male jcl: ICR mice were subjected to create wounds and treat with hydrogel containing pyroligneous extract at dose strengths of 0 (placebo), 100 and 150 µg/g-gel. Commercial gel (Intrasite^®) was used as an active comparator. On 3-, 7-, 10- and 14-day post-wounding, wound contraction was rated and wound site tissues were collected. The specimens were H&E stained and microscopically examined to evaluate histological responses. The underline wound healing related cytokine and polypeptide expressions were determined using real-time PCR and western blot.</p><p><strong>Results: </strong>It was found that the extract accelerated the healing process in a dose-dependent manner where at dose strength of 150 µg/g-gel was as effective as active comparator. It increased gene expression of the cytokine and related proteins in TGF-β/SMAD signaling pathway and may further activate diabetic induced TGF-β downregulation to restore up to the level that healthy skin tissues express. It also enhanced the expressions of Akt, FAK, RhoA and Rac-1 and evidently activated phosphorylation of Akt and FAK.</p><p><strong>Conclusion: </strong>The study demonstrated the extract could be a novel biomaterial for healing of such a chronic inflammatory wound as the wound in diabetes.</p>","PeriodicalId":11263,"journal":{"name":"Drug Development and Industrial Pharmacy","volume":" ","pages":"907-916"},"PeriodicalIF":2.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> evaluation of lipidic nanocarriers for mebendazole delivery to improve anticancer activity.","authors":"M Amin Tamma, Hamdi Nsairat, Mohamed El-Tanani, Razan Madi","doi":"10.1080/03639045.2024.2428405","DOIUrl":"10.1080/03639045.2024.2428405","url":null,"abstract":"<p><strong>Objective: </strong>To enhance the anticancer activity of the repurposed drug mebendazole (MBZ) against A549 cell lines by developing nanostructured lipid carriers (NLCs).</p><p><strong>Significance: </strong>MBZ, an anthelmintic drug, exhibits anticancer properties primarily through the inhibition of Ran GTPase and mitotic spindle assembly. Enhancing its delivery and efficacy via NLC could provide a novel and effective approach for lung cancer treatment.</p><p><strong>Methods: </strong>NLCs were prepared by mixing different ratios of solid lipid (stearic acid) and liquid lipid (oleic acid) with surfactants and emulsifiers. The NLCs were fully characterized to ensure stability, particle size, zeta potential, and encapsulation efficiency (EE%). The stability of the NLCs was monitored over a 3-week period. The anticancer activity of MBZ-NLCs was evaluated using IC₅₀ assays and <i>in vitro</i> scratch assays.</p><p><strong>Results: </strong>The NLCs exhibited an average particle size of 300 ± 10 nm and a zeta potential of -27 ± 0.5 mV, indicating good stability. EE% significantly improved from 40% in conventional liposome formulations to 90.7% in NLCs. The anticancer activity of MBZ-NLCs was markedly enhanced, with an IC₅₀ of 62 nM compared to 581 nM for free MBZ, representing a 10-fold increase in potency. Additionally, <i>in vitro</i> scratch assays revealed that MBZ-NLCs effectively prevented cell-cell contact, further supporting their potential for improved therapeutic efficacy.</p><p><strong>Conclusion: </strong>MBZ-NLCs exhibit significantly improved stability, EE%, and anticancer activity compared to free MBZ. These promising results suggest that MBZ-NLCs could be a potent therapeutic approach for lung cancer treatment, warranting further <i>in vivo</i> studies and exploration of different administration routes.</p>","PeriodicalId":11263,"journal":{"name":"Drug Development and Industrial Pharmacy","volume":" ","pages":"917-926"},"PeriodicalIF":2.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142616651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-administration of Ayurvedic medicines Arshogrit and Jatyadi Ghrit, attenuate croton oil-induced hemorrhoids in rat model of recto-anal inflammation by modulating TNF-α and IL-1β levels.","authors":"Acharya Balkrishna, Aakanksha Tiwari, Madhulina Maity, Meenu Tomer, Yash Varshney, Rishabh Dev, Sandeep Sinha, Anurag Varshney","doi":"10.1080/03639045.2024.2432595","DOIUrl":"10.1080/03639045.2024.2432595","url":null,"abstract":"<p><strong>Objective: </strong>To study the efficacy of co-administration of Arshogrit (AG) and Jatyadi Ghrit (JG), two herb-based Ayurvedic medicines, in rat model of croton oil-induced hemorrhoids.</p><p><strong>Significance: </strong>Hemorrhoids refer to a pathological condition affecting the recto-anal region causing pain, swelling, bleeding and protrusion. The available therapies for hemorrhoids are symptomatic or invasive but are expensive and associated with adverse effects. Hence, there exists a need for efficacious and safer pharmacotherapies.</p><p><strong>Methods: </strong>Ultra high performance liquid chromatography detected nine phytocompounds in AG and seven in JG. Seven fatty acids were additionally identified in JG by Gas Chromatography-Mass Spectrometry analysis. The in-vivo efficacy of the co-administration of AG, which was administered orally at the doses of 20, 60 and 200 mg/kg/day and JG, which was topically applied (100 mg/animal/day) was evaluated in Wistar rats by inducing hemorrhoids development with the application of croton oil preparation (COP) in the recto-anal area. Prednisolone was employed as the standard drug and was administered orally at the dose of 1 mg/kg/day.</p><p><strong>Results: </strong>AG and JG were able to attenuate the croton oil-induced macro and microscopic anomalies. Gross pathological observation demonstrated remarkable decrease in croton oil-induced swelling, hemorrhage and formation of pseudomembrane, with the escalating doses of AG. Microscopic observation revealed alleviation in the histopathological lesions (necrosis, inflammation, hemorrhage/congestion, degeneration and dilatation of blood vessels). AG and JG additionally reduced COP-induced increase in the serum levels of pro-inflammatory cytokines.</p><p><strong>Conclusion: </strong>This study convincingly demonstrates that co-administration of AG and JG is a potential therapy against hemorrhoids, warranting further investigations.</p>","PeriodicalId":11263,"journal":{"name":"Drug Development and Industrial Pharmacy","volume":" ","pages":"938-951"},"PeriodicalIF":2.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional Mongolian medicine Wu-Lan thirteen-flavor decoction protects rat from hypertension-induced renal injury via aryl hydrocarbon receptor-mediated pathway.","authors":"Xiaoli Du, Qianqian Tao, Siwen Fan, Jun Ren, Yu Dong, Gang Li, Shuang He, Xiaodong Cao, Yan Zhu","doi":"10.1080/03639045.2024.2432596","DOIUrl":"10.1080/03639045.2024.2432596","url":null,"abstract":"<p><strong>Background: </strong>Wu-Lan Thirteen-Flavour decoction (WLTd), a traditional Mongolian medicine, has been used for treating hypertension in clinical practice, but the chemical basis and underlying mechanisms remain unknown.</p><p><strong>Methods: </strong>The main components of WLTd were identified and quantified using HPLC and UPLC-MS/MS techniques. A compound-target-disease network was constructed using network pharmacology analysis to forecast the potential anti-hypertension targets. <i>In vivo</i> animal and <i>in vitro</i> cellular experiments were performed to validate the efficacy and molecular mechanisms of renal protection of WLTd and its main active components in spontaneous hypertension.</p><p><strong>Results: </strong>A total of 136 active compounds in WLTd were collected through relevant databases, and network pharmacology analysis identified that the aryl hydrocarbon receptor (AhR) signaling pathway may serve as a potential anti-hypertension targets. Eight of the active components, including vitexin, kaempferol, toosendanin, ursolic acid, matrine, oxymatrine, gardenoside and quercetin, were identified and quantified by HPLC and UPLC-MS/MS. WLTd effectively lowered the mean blood pressure (159.16 ± 13.91 vs 135 ± 13.37 mmHg), reduced the BUN (391.55 ± 59.96 vs 240.88 ± 51.15 mmol/L) and creatinine (1.78 ± 0.41 vs 0.67 ± 0.34 nmol/L) levels, and reduced hypertension-induced renal damage in SHR. AhR and related key gene expression changes predicted by network pharmacology analysis were validated by immunohistochemistry, RT-qPCR, and Western blot analyses. <i>In vitro,</i> studies also showed that WLTd up-regulated AhR expression in angiotensin II-induced HEK293 cell injury.</p><p><strong>Conclusions: </strong>Wu-Lan Thirteen-Flavour decoction effectively protects hypertension-induced renal injury by regulating the Aryl Hydrocarbon Receptor signaling pathway.</p>","PeriodicalId":11263,"journal":{"name":"Drug Development and Industrial Pharmacy","volume":" ","pages":"952-967"},"PeriodicalIF":2.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly synthesis of silver nanoparticles by <i>Trigonella foenum-graecum</i>: formulations, characterizations, and application in wound healing.","authors":"Amal Al-Subaiyel, Ahmed A H Abdellatif","doi":"10.1080/03639045.2024.2431934","DOIUrl":"https://doi.org/10.1080/03639045.2024.2431934","url":null,"abstract":"<p><strong>Background: </strong>Due to the toxicity and serious side effects of chemical incorporated in topical dosage form used for treatment of wound healing, there is a need to use natural preparation as wound healing preparation.</p><p><strong>Aims: </strong>Seeds of <i>Trigonella foenum-graecum</i> (TFG) are used to synthesize eco-friendly silver nanoparticles (SNPs) in an appropriate way to heal wounds.</p><p><strong>Methods: </strong>To synthesize SNPs, TFG was incubated with AgNO₃ to produce SNP-TFG. The obtained SNP-TFG was characterized for their wavelength, size and ζ-potential, surface morphology, and yield production. Then, SNP-TFG was formulated as a topical cream (O/W), characterized, and applied to the rats' groups to examine its wound-healing activity. Finally, a skin biopsy was performed to assess all rats' immunostaining and histopathological (HP) alterations in skin lesions on days 3, 7, 10, and 14.</p><p><strong>Results: </strong>The prepared SNP-TFG showed non-aggregated nano-formulation, with a λ_max of 396 nm. SNP-TFG recorded a size of 43.65 ± 2.1 nm, a charge of -15.03 ± 3.2 mV, and showed yield of 52.61 ± 1.41% while the release was continued for more than 12 h. During the biosynthesis process, the compounds present in TFG are capable of reducing silver ions (Ag⁺) to form SNPs. SNP-TFG cream showed a pH nearly equal to the skin's pH, with suitable viscosity and homogeneity and an apparent permeability of 0.009 ± 0.001. Further, the HP of the SNP-TFG showed a substantial reduction in wound mass, wound granulation tissue growth enhancement, and epidermal re-epithelialization (proliferation) compared to the control group.</p><p><strong>Conclusion: </strong>The obtained SNP-TFG is considered a novel skin wound-healing natural and eco-friendly nano-formulation.</p>","PeriodicalId":11263,"journal":{"name":"Drug Development and Industrial Pharmacy","volume":"50 11","pages":"927-937"},"PeriodicalIF":2.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}