Diseases of aquatic organisms最新文献_第3页

Experimental challenge of East Asian bullfrog Hoplobatrachus rugulosus tadpoles with tiger frog virus: dosage impact on disease severity. 虎蛙病毒攻毒东亚牛蛙小蝌蚪实验：剂量对疾病严重程度的影响。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2026-02-12 DOI: 10.3354/dao03896

Preeyanan Sriwanayos, Robert J Ossiboff, Somkiat Kanchanakhan, Jaree Polchana, Kamonchai Imnoi, Pedro H O Viadanna, Thais C S Rodrigues, James Colee, Thomas B Waltzek

{"title":"Experimental challenge of East Asian bullfrog Hoplobatrachus rugulosus tadpoles with tiger frog virus: dosage impact on disease severity.","authors":"Preeyanan Sriwanayos, Robert J Ossiboff, Somkiat Kanchanakhan, Jaree Polchana, Kamonchai Imnoi, Pedro H O Viadanna, Thais C S Rodrigues, James Colee, Thomas B Waltzek","doi":"10.3354/dao03896","DOIUrl":"10.3354/dao03896","url":null,"abstract":"Ranaviruses are associated with epizootics in poikilothermic vertebrates worldwide. The ranavirus tiger frog virus (TFV) causes lethal, systemic disease in amphibians and reptiles. TFV was associated with mortality in East Asian bullfrogs Hoplobatrachus rugulosus (EAB) of all developmental stages in ranaculture facilities in Thailand between the late 1990s and late 2010s. However, experimental evidence of the pathogenic potential of TFV in EAB has not previously been reported. The objective of this study was to determine the susceptibility of EAB tadpoles to TFV under controlled laboratory conditions. We conducted bath challenges at 2 viral dosages, 5 × 104 and 5 × 106 TCID50 virus ml-1, and monitored cumulative mortality for 21 d. Low to moderate (33%) mortality was observed among tadpoles exposed to a low dosage inoculum, while high (71%) mortality was observed among tadpoles exposed to a high dosage. All tadpoles that died or were euthanized in the high dosage treatment during the 21 d study period tested positive by qPCR and virus isolation, with homogenates yielding high viral loads as measured by qPCR. Histopathology, aided by the development of a frog virus 3 (FV3)-specific in situ hybridization probe, revealed the expected microscopic lesions in EAB tadpoles exposed to TFV that died during the experiment, but they were absent in unexposed control tadpoles. The pathogenicity of TFV in this highly valued food species underscores the need for improved biosecurity measures and mitigation strategies to protect the Thai ranaculture industry from TFV.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"165 ","pages":"1-14"},"PeriodicalIF":1.2,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147289619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ranavirus infection in anuran species in Atlantic Forest fragments in Brazil. 巴西大西洋森林片段中无尾猴的Ranavirus感染。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2026-01-29 DOI: 10.3354/dao03892

Julia Anselmo da Luz Rico, Aline Almeida Fonseca, Pedro Henrique de Oliveira Viadanna, Jesse L Brunner, Karla Magalhães Campião

{"title":"Ranavirus infection in anuran species in Atlantic Forest fragments in Brazil.","authors":"Julia Anselmo da Luz Rico, Aline Almeida Fonseca, Pedro Henrique de Oliveira Viadanna, Jesse L Brunner, Karla Magalhães Campião","doi":"10.3354/dao03892","DOIUrl":"https://doi.org/10.3354/dao03892","url":null,"abstract":"Ranaviruses (RVs) are emerging pathogens that infect amphibians, reptiles, and fish, and have been linked to declines in several anuran species globally. Assessing their prevalence in wild populations is essential for effective monitoring and conservation efforts. This study evaluates the prevalence of RV in anurans from forest fragments in the Atlantic Forest in Brazil. We investigated the presence of RV in tissue samples obtained from 203 anurans, which included metamorphosed individuals and tadpoles of native species and the invasive American bullfrog. Additionally, we collected water from ponds to assess the presence of RV in the environment (using eDNA). We performed quantitative real-time PCR assays targeting the major capsid protein (MCP)-encoding region of RV to evaluate the potential risk posed by RV within these communities. The analysis revealed the presence of viral DNA in 3 of 5 sites and an overall prevalence of 60% in the animals. Our results show that RV is widespread and prevalent among wild amphibians living in fragments of the Atlantic Forest. Our findings highlight the potential threat posed by RVs as an emerging disease.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"165 ","pages":"1-11"},"PeriodicalIF":1.2,"publicationDate":"2026-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146084821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-infection of two genotypes of decapod hepanhamaparvovirus 1 (DHPV) in Penaeus vannamei from South Korea. 两种基因型乙型肝炎病毒1型在韩国南美对虾中的联合感染。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2026-01-29 DOI: 10.3354/dao03891

Myoung Gwang Choi, Soon Joo Hong, Ki Hong Kim

{"title":"Co-infection of two genotypes of decapod hepanhamaparvovirus 1 (DHPV) in Penaeus vannamei from South Korea.","authors":"Myoung Gwang Choi, Soon Joo Hong, Ki Hong Kim","doi":"10.3354/dao03891","DOIUrl":"https://doi.org/10.3354/dao03891","url":null,"abstract":"Decapod hepanhamaparvovirus 1 (DHPV), formerly known as hepatopancreatic parvovirus (HPV), primarily causes stunted growth in juvenile shrimp and, in some cases, mortality in shrimp larvae. DHPV belongs to the genus Hepanhamaparvovirus within the family Parvoviridae and is classified into 5 genotypes (genotypes I-V). Previous studies have reported DHPV infections in various shrimp species; however, no reports have documented co-infection with different DHPV genotypes in shrimp. In this study, we report the first evidence of co-infection by 2 DHPV genotypes-genotype IV (DHPV-GS) and genotype V (DHPV-GJ)-in Penaeus vannamei cultured in South Korea. To discriminate between the 2 genotypes, we established and validated genotype-specific PCR assays, and the assays demonstrated high specificity, with no cross-amplification observed between the 2 genotypes. Using these assays, we screened 38 shrimp from Goseong and 12 shrimp from Geoje, and found co-infection with both DHPV-GS and DHPV-GJ in 36.8% and 100% of the samples, respectively. These findings highlight the importance of genotype-specific diagnosis for accurate surveillance and improved understanding of DHPV epidemiology in aquaculture systems.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"165 ","pages":"1-6"},"PeriodicalIF":1.2,"publicationDate":"2026-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146084804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eimeria in the Xochimilco axolotl Ambystoma mexicanum.

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2026-01-29 DOI: 10.3354/dao03893

Adriana Vázquez-Carlos, Fernando Gual-Sill, Osvaldo López-Díaz, Emilio Rendón-Franco, Claudia I Muñoz-García

引用次数: 0

First confirmed case of malignant melanoma in Terapon jarbua from Indian coastal water. 印度沿海水域发现首例恶性黑色素瘤确诊病例。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2026-01-29 DOI: 10.3354/dao03894

Vasanthan Koothan, Nguyen Dinh-Hung, Singaravel Vijayapoopathi, Ayyaru Gopalakrishnan

{"title":"First confirmed case of malignant melanoma in Terapon jarbua from Indian coastal water.","authors":"Vasanthan Koothan, Nguyen Dinh-Hung, Singaravel Vijayapoopathi, Ayyaru Gopalakrishnan","doi":"10.3354/dao03894","DOIUrl":"https://doi.org/10.3354/dao03894","url":null,"abstract":"Melanoma is a highly aggressive neoplasm of melanocytes, commonly studied in mammals but rarely documented in wild fish populations. This study reports the first confirmed case of malignant melanoma in Terapon jarbua (Forsskål, 1775) from the Parangipettai landing center on the southeastern coast of India. Gross examination revealed superficial raised, hyperpigmented lesions primarily distributed along the dorsal and lateral body surfaces. Wet mount analysis showed extensive melanization within the dermal layers of the affected tissue. Histological examination identified pleomorphic melanocytes with dense melanin deposition, architectural disruption of the skin, and features consistent with superficially spreading melanoma. Scanning electron microscopy of melanotic lesions revealed significant alterations to the epidermal surface, with rounded or angular projections and sharply defined crevices. Transmission electron microscopy showed classical ultrastructural abnormalities such as pleomorphism, irregular melanosome aggregation, binucleation, cytoplasmic vacuolation, and necrotic cells with compromised plasma membranes. The presence of melanosomes at various maturation stages indicated hyperactive melanogenic activity. This novel discovery points to the importance of regular tumour surveillance and research into environmental stressors as possible causative agents, adding to the small but increasing body of evidence of neoplastic disease in Indian marine ecosystems.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"165 ","pages":"1-11"},"PeriodicalIF":1.2,"publicationDate":"2026-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146084831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Piscine orthoreovirus genotype 3b (PRV-3b) in rainbow trout Oncorhynchus mykiss farmed in Peru. 秘鲁养殖虹鳟鱼正呼肠孤病毒基因型3b （PRV-3b）

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2026-01-29 DOI: 10.3354/dao03895

Romina Caballero-Celli, Victoria Jaramillo, Ricardo Ildefonso, Rodolfo Velazco-Peña, José Rodriguez-Callan, Francisco Ulloa-Stanojlovic, Muriel Gómez-Sánchez, Marco Rozas-Serri

{"title":"Piscine orthoreovirus genotype 3b (PRV-3b) in rainbow trout Oncorhynchus mykiss farmed in Peru.","authors":"Romina Caballero-Celli, Victoria Jaramillo, Ricardo Ildefonso, Rodolfo Velazco-Peña, José Rodriguez-Callan, Francisco Ulloa-Stanojlovic, Muriel Gómez-Sánchez, Marco Rozas-Serri","doi":"10.3354/dao03895","DOIUrl":"https://doi.org/10.3354/dao03895","url":null,"abstract":"Piscine orthoreovirus (PRV) infection is widespread in the global salmonid aquaculture industry. This study aimed to investigate the presence or absence of PRV genotypes in rainbow trout Oncorhynchus mykiss farmed in Peru. The Peruvian National Authority for Fisheries and Aquaculture Health and Safety (SANIPES) carried out a disease surveillance plan between October and December 2023. In this context, 48 fish exhibiting clinical signs of circulatory disorders were screened for PRV genotypes. Quantitative PCR analysis showed that 12.5% of pools (2 out of 16) were positive for PRV-3 but negative for PRV-1 and PRV-2. The CT value was 23.48 for tissue pool no. 14 and 27.03 for tissue pool no. 15, but both pools contained tissues from fish farmed in Lake Titicaca. Concurrently, Weissella ceti was isolated from these fish. PRV-3-positive fish showed mild mononuclear myocarditis in the ventricular spongy layer (2 out of 6). The sequences of the S1 and M2 segments of the Peruvian isolates belonged to the PRV-3 gene group, subgroup PRV-3b. These sequences showed 100% identity with the Danish PRV-3b isolates, suggesting possible European origin. Overall, the results of this study confirm, for the first time, the presence of PRV-3b in the Peruvian rainbow trout farming industry and highlight the need to implement biosecurity measures for the import of embryonic eggs to minimize the risk of pathogen entry and dissemination in the industry, as well as a permanent epidemiological surveillance plan for all PRV genotypes.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"165 ","pages":"1-12"},"PeriodicalIF":1.2,"publicationDate":"2026-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146084884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Voriconazole is an effective treatment for clinical chytridiomycosis in two Critically Endangered amphibians. 伏立康唑是治疗两种极危两栖动物壶菌病的有效药物。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2026-01-15 DOI: 10.3354/dao03889

Daniel J Guinto, Melissa Hernández-Poveda, Amy Aquilina, Mikaeylah J Davidson, Lee F Skerratt, Tiffany A Kosch, Lee Berger

{"title":"Voriconazole is an effective treatment for clinical chytridiomycosis in two Critically Endangered amphibians.","authors":"Daniel J Guinto, Melissa Hernández-Poveda, Amy Aquilina, Mikaeylah J Davidson, Lee F Skerratt, Tiffany A Kosch, Lee Berger","doi":"10.3354/dao03889","DOIUrl":"10.3354/dao03889","url":null,"abstract":"Voriconazole treatment for amphibian chytridiomycosis has emerged as a less toxic alternative to the widely used itraconazole, although its efficacy needs to be validated across different species, particularly for frogs with clinical signs of disease. We assessed the effectiveness of topical voriconazole antifungal treatments for subclinical and clinical chytrid infections after experimental Batrachochytrium dendrobatidis (Bd) exposure. In addition, we looked to validate this treatment for use with multiple age classes of 2 highly susceptible, Critically Endangered Australian myobatrachid frogs: southern corroboree frogs Pseudophryne corroboree and northern corroboree frogs P. pengilleyi. Treatment involved twice daily topical application of a 0.00015% voriconazole solution for 7 d or until clear of Bd. Supportive care was provided for frogs displaying clinical signs of chytridiomycosis. This study was conducted across 2 experiments: experiment 1 (Expt 1) involved only P. corroboree and Expt 2 included P. corroboree and P. pengilleyi. In Expt 1, voriconazole treatment cleared Bd from 78% (N = 21/27) of infected P. corroboree, resulting in 68% survival among the P. corroboree with clinical chytridiomycosis. In Expt 2, 100% of Bd-positive P. pengilleyi (N = 7) cleared and survived infection, including all 4 cases with clinical disease. In Expt 2, none of the P. corroboree (4) tested positive for Bd, and all remained normal throughout treatment. Our results demonstrate that voriconazole is an effective treatment for chytridiomycosis in P. corroboree and P. pengilleyi, and that clinically ill frogs of multiple age classes can recover with the addition of electrolyte therapy and antibiotics.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"165 ","pages":"1-10"},"PeriodicalIF":1.2,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145970752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an mRNA-specific real-time PCR for the detection of replicating cyprinid herpesvirus-3 (CyHV-3) in carp and non-target species. 在鲤鱼和非靶种中复制CyHV-3的mrna特异性实时PCR检测方法的建立。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2026-01-15 DOI: 10.3354/dao03887

R Klein, D M Cummins, P G Mohr, N J G Moody

{"title":"Development of an mRNA-specific real-time PCR for the detection of replicating cyprinid herpesvirus-3 (CyHV-3) in carp and non-target species.","authors":"R Klein, D M Cummins, P G Mohr, N J G Moody","doi":"10.3354/dao03887","DOIUrl":"10.3354/dao03887","url":null,"abstract":"Cyprinid herpesvirus 3 (CyHV-3) is a highly contagious virus that causes high mortalities in common and koi carp worldwide. The molecular detection of this double-stranded DNA virus has been extensively researched. Nonetheless, there are currently no real-time PCR assays available for detecting CyHV-3 mRNA, which could serve as an indicator of active virus replication, aiding in the evaluation of the susceptibility of non-target species. This study describes a probe-based reverse transcription, real-time PCR (RT-qPCR) assay that was designed to detect CyHV-3 mRNA for efficient, high-throughput detection. The assay is highly specific for CyHV-3 mRNA, with no detection in samples from non-infected fish, closely related viruses or CyHV-3 DNA. The analytical sensitivity of the assay was examined using dilutions of a plasmid control and nucleic acid from a CyHV-3-infected cell line, where the CyHV-3 mRNA limit of detection was approximately 1 copy per reaction. Testing of diluted CyHV-3 mRNA demonstrated comparable sensitivity of the RT-qPCR with an existing reverse transcription PCR assay. Progressive monitoring of positive control samples revealed that the assay had a high level of repeatability. The assay was used to provide further evidence that non-target species silver perch and Murray cod were not susceptible to developing disease when experimentally exposed to CyHV-3. The novel RT-qPCR assay is an invaluable tool for detection of the replication phase of CyHV-3.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"165 ","pages":"1-12"},"PeriodicalIF":1.2,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145970732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic entanglement of an immature bottlenose dolphin in fishing gear in the northern Adriatic Sea, with intervention implications. 在亚得里亚海北部，一只未成熟的宽吻海豚在渔具中长期纠缠，具有干预意义。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2026-01-15 DOI: 10.3354/dao03890

Tilen Genov, Tomislav Paller

引用次数: 0

Dynamics of fibropapillomatosis in green turtles Chelonia mydas from Babitonga Bay, southern Brazil. 巴西南部巴比通加湾绿龟纤维乳头状瘤病的动态。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-12-18 DOI: 10.3354/dao03886

Tiago Fernando Alves, Marta Jussara Cremer

{"title":"Dynamics of fibropapillomatosis in green turtles Chelonia mydas from Babitonga Bay, southern Brazil.","authors":"Tiago Fernando Alves, Marta Jussara Cremer","doi":"10.3354/dao03886","DOIUrl":"https://doi.org/10.3354/dao03886","url":null,"abstract":"Green turtles Chelonia mydas are vital components of marine ecosystems and are recognized as indicators of environmental health. Fibropapillomatosis (FP), a debilitating disease associated with a chelonid herpesvirus, disproportionately affects juveniles in coastal foraging areas. This study presents the first analysis of FP dynamics in Babitonga Bay, southern Brazil, an important habitat for the species. Between 2019 and 2023, 171 juvenile green turtles were monitored using capture-mark-recapture and photo-identification techniques. FP prevalence in Babitonga Bay was among the highest reported in Brazil, reaching 53.2%. Tumors were predominantly located in the anterior region of the body, particularly around the flippers and axillary area, and were mostly small, consistent with mild FP. Disease severity was quantified using an FP index, and its association with body condition was examined, revealing no significant association. Data from recaptured individuals revealed both tumor progression and regression, underscoring individual variability and suggesting the influence of environmental and immunological factors. The predominance of FP in juveniles highlights their heightened vulnerability during early developmental stages. These findings enhance our understanding of FP dynamics in green turtles and support the need for continued health assessments and conservation measures. This research contributes to global efforts to safeguard marine biodiversity, aligning with the goals of the United Nations Decade of Ocean Science for Sustainable Development.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"164 ","pages":"189-203"},"PeriodicalIF":1.2,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145773913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0