Diseases of aquatic organisms最新文献_第3页

RNA interference targeting WSSV ribonucleotide reductase 2 provides long-term protection against infection in Litopenaeus vannamei. 以 WSSV 核糖核苷酸还原酶 2 为靶点的 RNA 干扰可提供长期保护，防止万年青感染。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-08-15 DOI: 10.3354/dao03805

Soon Joo Hong, Ki Hong Kim

{"title":"RNA interference targeting WSSV ribonucleotide reductase 2 provides long-term protection against infection in Litopenaeus vannamei.","authors":"Soon Joo Hong, Ki Hong Kim","doi":"10.3354/dao03805","DOIUrl":"10.3354/dao03805","url":null,"abstract":"Many studies have demonstrated that long double-stranded RNAs (dsRNAs) targeting essential genes of white spot syndrome virus (WSSV) can induce a sequence-specific antiviral RNA interference (RNAi) response in shrimp, thereby offering protection against WSSV infection. However, further experimental data on the required dose of dsRNAs and the duration of protection from a single administration are necessary to establish RNAi-mediated methods as effective and practical antiviral measures. In this study, we evaluated the protective efficacy and the duration of protection provided by a single administration of various doses of long dsRNA targeting WSSV ribonucleotide reductase 2 (rr2) in white-leg shrimp Litopenaeus vannamei. The protective efficacy of long dsRNA targeting WSSV rr2 was not diminished by the reduction of the dose to 100 ng g-1 of body weight, suggesting that a relatively low dose can effectively induce an RNAi response in shrimp. Furthermore, shrimp were well-protected against WSSV challenges for up to 4 wk post-administration of the rr2-targeting long dsRNA, although the protective effect almost disappeared at 6 wk post-administration. These results suggest that long dsRNAs can provide protection against WSSV for at least 1 mo, and monthly administration of long dsRNAs could serve as a long-term protective strategy for shrimp against WSSV.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"159 ","pages":"71-78"},"PeriodicalIF":1.1,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141981960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of buccal cavity parasite Ceratothoa carinata (Isopoda, Cymothoidae) on the condition and reproduction of its host fish Japanese scad Decapterus maruadsi. 颊腔寄生虫 Ceratothoa carinata（等足目，鲤科）对宿主鱼日本鳞鯵的状态和繁殖的影响。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-08-15 DOI: 10.3354/dao03806

Reina Nakano, Yuzumi Okumura, Hiroki Hata

引用次数: 0

Regeneration of tunic cuticle is suppressed in edible ascidian Halocynthia roretzi contracting soft tunic syndrome. 食用腹足纲 Halocynthia roretzi 感染软鳞茎皮综合征后，鳞茎皮的再生受到抑制。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-08-01 DOI: 10.3354/dao03801

Kei Nakayama, Yumiko Obayashi, Leo Munechika, Shin-Ichi Kitamura, Tetsuya Yanagida, Miho Honjo, Shoko Murakami, Euichi Hirose

{"title":"Regeneration of tunic cuticle is suppressed in edible ascidian Halocynthia roretzi contracting soft tunic syndrome.","authors":"Kei Nakayama, Yumiko Obayashi, Leo Munechika, Shin-Ichi Kitamura, Tetsuya Yanagida, Miho Honjo, Shoko Murakami, Euichi Hirose","doi":"10.3354/dao03801","DOIUrl":"https://doi.org/10.3354/dao03801","url":null,"abstract":"Soft tunic syndrome is an infectious disease caused by the flagellate Azumiobodo hoyamushi, which severely damages the aquaculture of the edible ascidian Halocynthia roretzi. Tunic is a cellulosic extracellular matrix entirely covering the body in ascidians and other tunicates, and its dense cuticle layer covers the tunic surface as a physical barrier against microorganisms. When the tunic of intact H. roretzi individuals was cut into strips, electron-dense fibers (DFs) appeared on the cut surface of the tunic matrix and aggregated to regenerate a new cuticular layer in seawater within a few days. DF formation was partially or completely inhibited in individuals with soft tunic syndrome, and DF formation was also inhibited by the presence of some proteases, indicating the involvement of proteolysis in the process of tunic softening as well as cuticle regeneration. Using pure cultures of the causative flagellate A. hoyamushi, the expression of protease genes and secretion of some proteases were confirmed by RNA-seq analysis and a 4-methylcoumaryl-7-amide substrate assay. Some of these proteases may degrade proteins in the tunic matrix. These findings suggest that the proteases of A. hoyamushi is the key to understanding the mechanisms of cuticular regeneration inhibition and tunic softening.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"159 ","pages":"37-48"},"PeriodicalIF":1.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Countrywide screening supports model-based predictions of the distribution of Batrachochytrium dendrobatidis in Ukraine. 全国范围的筛查支持基于模型的乌克兰蝙蝠病分布预测。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-08-01 DOI: 10.3354/dao03802

Joanna Jakóbik, Mykola Drohvalenko, Eduardo Fernandez Melendez, Emilia Kępa, Oleksandra Klynova, Anna Fedorova, Oleksii Korshunov, Oleksii Marushchak, Oksana Nekrasova, Natalia Suriadna, Nazar Smirnov, Oksana Tkachenko, Andrii Tupikov, Christophe Dufresnes, Oleksandr Zinenko, Maciej Pabijan

{"title":"Countrywide screening supports model-based predictions of the distribution of Batrachochytrium dendrobatidis in Ukraine.","authors":"Joanna Jakóbik, Mykola Drohvalenko, Eduardo Fernandez Melendez, Emilia Kępa, Oleksandra Klynova, Anna Fedorova, Oleksii Korshunov, Oleksii Marushchak, Oksana Nekrasova, Natalia Suriadna, Nazar Smirnov, Oksana Tkachenko, Andrii Tupikov, Christophe Dufresnes, Oleksandr Zinenko, Maciej Pabijan","doi":"10.3354/dao03802","DOIUrl":"https://doi.org/10.3354/dao03802","url":null,"abstract":"The chytrid Batrachochytrium dendrobatidis (Bd) is a widespread fungus causing amphibian declines across the globe. Although data on Bd occurrence in Eastern Europe are scarce, a recent species distribution model (SDM) for Bd reported that western and north-western parts of Ukraine are highly suitable to the pathogen. We verified the SDM-predicted range of Bd in Ukraine by sampling amphibians across the country and screening for Bd using qPCR. A total of 446 amphibian samples (tissue and skin swabs) from 11 species were collected from 36 localities. We obtained qPCR-positive results for 33 samples including waterfrogs (Pelophylax esculentus complex) and fire- and yellow-bellied toads (Bombina spp.) from 8 localities. We found that Bd-positive localities had significantly higher predicted Bd habitat suitability than sites that were pathogen-free. Amplification and sequencing of the internal transcribed spacer (ITS) region of samples with the highest Bd load revealed matches with ITS haplotypes of the globally distributed BdGPL strain, and a single case of the BdASIA-2/BdBRAZIL haplotype. We found that Bd was non-randomly distributed across Ukraine, with infections present in the western and north-central forested peripheries of the country with a relatively cool, moist climate. On the other hand, our results suggest that Bd is absent or present in low abundance in the more continental central, southern and eastern regions of Ukraine, corroborating the model-predicted distribution of chytrid fungus. These areas could potentially serve as climatic refugia for Bd-susceptible amphibian hosts.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"159 ","pages":"15-27"},"PeriodicalIF":1.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Verminous bronchitis and pneumonia by nasal trematodes in Greater Caribbean manatees from Puerto Rico. 波多黎各大加勒比海蝠鲼鼻腔吸虫引起的虫性支气管炎和肺炎。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-08-01 DOI: 10.3354/dao03800

Carla I Rivera-Pérez, Antonio A Mignucci-Giannoni, Mark A Freeman, Juan M Orcera-Iglesias, Lesly J Cabrias-Contreras, Michelle M Dennis

{"title":"Verminous bronchitis and pneumonia by nasal trematodes in Greater Caribbean manatees from Puerto Rico.","authors":"Carla I Rivera-Pérez, Antonio A Mignucci-Giannoni, Mark A Freeman, Juan M Orcera-Iglesias, Lesly J Cabrias-Contreras, Michelle M Dennis","doi":"10.3354/dao03800","DOIUrl":"https://doi.org/10.3354/dao03800","url":null,"abstract":"Five adult Greater Caribbean manatees Trichechus manatus manatus were found stranded on various coasts of Puerto Rico; 2 stranded alive and 3 stranded dead. Clinical signs observed in live-stranded manatees included emaciation, weakness, bradypnea, arrhythmia, and nasal mucus discharge. Postmortem examinations revealed serosanguinous, mucohemorrhagic, or suppurative exudate in bronchi associated with luminal adult Pulmonicola cochleotrema (range: 18-182 trematodes), accompanied by pulmonary abscesses in 2 cases. Histologically, we observed eosinophilic bronchopneumonia of varying severity (n = 4) and chronic erosive to eosinophilic tracheobronchitis (n = 4) with squamous metaplasia (n = 3) and intralesional trematodes and eggs. The trematode identity was confirmed and compared through molecular analysis for the amplified 18S rDNA fragment. Comorbidities included enteric chiorchosis (n = 5), gastric heterocheilosis (n = 4), malnutrition (n = 4), trauma related to watercraft collision (n = 3), systemic toxoplasmosis (n = 1), acute bacterial peritonitis (n = 1), and interstitial nephritis (n = 1), suggesting that immunosuppression was a predisposing factor for lower respiratory tract pulmonicolosis. Based on lesion severity, clinical signs, and the presence and absence of other findings to explain death, this condition was considered the primary cause of death in 1 manatee, a contributory cause of death in 3 manatees, and an incidental finding in 1 individual. These clinicopathological descriptions will facilitate the diagnosis and clinical management of pulmonicolosis in T. manatus, a species endangered with extinction.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"159 ","pages":"49-63"},"PeriodicalIF":1.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Setting epidemiological cut-off values relevant to MIC and disc diffusion data for Aeromonas salmonicida generated by a standard method. 根据标准方法得出的沙门单胞菌 MIC 和盘扩散数据设定流行病学临界值。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-08-01 DOI: 10.3354/dao03798

Peter Smith, Elizabeth Buba, Andrew P Desbois, Alexandra Adams, David Verner-Jeffreys, Andrew Joseph, Edel Light, Laëtitia Le Devendec, Eric Jouy, Emeline Larvor, Pierre-Marie Boitard, Matthieu Jamin, Nicolas Keck, Alain Le Breton, Benoit Thuillier, Christian Ravaille, Thierry Morin, Sandrine Baron

{"title":"Setting epidemiological cut-off values relevant to MIC and disc diffusion data for Aeromonas salmonicida generated by a standard method.","authors":"Peter Smith, Elizabeth Buba, Andrew P Desbois, Alexandra Adams, David Verner-Jeffreys, Andrew Joseph, Edel Light, Laëtitia Le Devendec, Eric Jouy, Emeline Larvor, Pierre-Marie Boitard, Matthieu Jamin, Nicolas Keck, Alain Le Breton, Benoit Thuillier, Christian Ravaille, Thierry Morin, Sandrine Baron","doi":"10.3354/dao03798","DOIUrl":"https://doi.org/10.3354/dao03798","url":null,"abstract":"The Clinical and Laboratory Standards Institute has published epidemiological cut-off values for susceptibility data generated at 22°°C and read after 44-48 h for florfenicol, oxolinic acid and oxytetracycline against Aeromonas salmonicida. The cut-off values for the minimum inhibitory concentration (MIC) and disc diffusion were derived from data obtained by 1 laboratory and 2 laboratories respectively. The present work reports the generation of susceptibility data from additional laboratories and the calculation of provisional cut-off values from aggregations of these data with previously published data. With respect to MIC data, the provisional cut-off values, derived from aggregations of the data from 4 laboratories, were ≤4 µg ml-1 for florfenicol, ≤0.0625 µg ml-1 for oxolinic acid and ≤1 µg ml-1 for oxytetracycline. For disc diffusion data, the provisional cut-off values derived from aggregations of the data from 5 laboratories were ≥30 mm for florfenicol, ≥32 mm for oxolinic acid and ≥25 mm for oxytetracycline. In addition, a cut-off value of ≥29 mm for ampicillin was derived from the aggregation of data from 4 laboratories.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"159 ","pages":"29-35"},"PeriodicalIF":1.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Voriconazole successfully treats chytridiomycosis in frogs. 伏立康唑成功治疗了青蛙糜烂性真菌病。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-07-11 DOI: 10.3354/dao03795

Laura A Brannelly, Preeti Sharma, Sofia Ballesteros, Alexander S Wendt

引用次数: 0

The first intermediate host of the invasive frog trematode Glypthelmins quieta in Japan. 入侵蛙类震颤虫 Glypthelmins quieta 在日本的第一个中间宿主。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-07-11 DOI: 10.3354/dao03799

Eriko Ansai, Masato Nitta, Takumi Saito, Yosuke Kojima, Tsukasa Waki

引用次数: 0

Pennella balaenoptera actively select injured cetacean skin as attachment sites, making them potentially useful forensic tags. 巴拉蝠鲼会主动选择受伤的鲸类皮肤作为附着点，这使它们成为可能有用的法医标签。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-06-27 DOI: 10.3354/dao03796

O Chaieb, S Ten, F J Aznar

{"title":"Pennella balaenoptera actively select injured cetacean skin as attachment sites, making them potentially useful forensic tags.","authors":"O Chaieb, S Ten, F J Aznar","doi":"10.3354/dao03796","DOIUrl":"https://doi.org/10.3354/dao03796","url":null,"abstract":"Cetaceans harbor multiple epibionts on their external surface, and these attach to particular microhabitats. Understanding what drives the selection of attachment sites is relevant for refining the use of epibionts as indicators of their hosts. We report on about 100 females of the mesoparasitic copepod Pennella balaenoptera attached to a dead Cuvier's beaked whale Ziphius cavirostris stranded in Tunisia (western Mediterranean); the first report of P. balaenoptera in this country. The copepods were exclusively attached to numerous incisive, likely anthropogenic, wounds found on the host's skin. This finding suggests that newly recruited females may actively seek skin areas where physical penetration is facilitated; a factor that may help explain patterns of microhabitat selection by Pennella spp., and perhaps other pennellids, on their hosts. The estimated age of parasitization by P. balaenoptera (supported by age estimations of the co-occurring epibiotic barnacle Conchoderma virgatum) also suggests that the cetacean host likely survived these injuries, at least initially, and the presumed cause of death was starvation due to entanglement in a fishing net.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"158 ","pages":"195-200"},"PeriodicalIF":1.1,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141455882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Red and melanized focal changes in the white skeletal muscle of farmed rainbow trout Oncorhynchus mykiss. 养殖虹鳟鱼白色骨骼肌中的红色和黑色病灶变化。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-06-27 DOI: 10.3354/dao03797

Håvard Bjørgen, Malin Brimsholm, Morten Lund, Maria K Dahle, Espen Rimstad, Erling Olaf Koppang

{"title":"Red and melanized focal changes in the white skeletal muscle of farmed rainbow trout Oncorhynchus mykiss.","authors":"Håvard Bjørgen, Malin Brimsholm, Morten Lund, Maria K Dahle, Espen Rimstad, Erling Olaf Koppang","doi":"10.3354/dao03797","DOIUrl":"https://doi.org/10.3354/dao03797","url":null,"abstract":"Fillet discoloration by red and melanized focal changes (RFCs and MFCs) is common in farmed Atlantic salmon Salmo salar. In farmed rainbow trout Oncorhynchus mykiss, similar changes have been noted, but their prevalence and histological characteristics have not been investigated. Thus, we conducted a study encompassing 1293 rainbow trout from 3 different farm sites in Norway, all examined at the time of slaughter. Both macroscopic and histological assessments of the changes were performed. Reverse transcription (RT)-qPCR analyses and in situ hybridization (ISH) were used to detect the presence and location, respectively, of potential viruses. Only 1 RFC was detected in a single fillet, while the prevalence of MFCs ranged from 1.46 to 6.47% between populations. The changes were predominantly localized in the cranioventral region of the fillet. Histological examinations unveiled necrotic myocytes, fibrosis, and regeneration of myocytes. Melano-macrophages were found in the affected areas and in myoseptal adipose tissue. Organized granulomas were observed in only 1 fish. Notably, the presence of inflammatory cells, including melano-macrophages, appeared lower compared to what has been previously documented in Atlantic salmon MFCs. Instead, fibrosis and regeneration dominated. RT-qPCR and ISH revealed the presence of piscine orthoreovirus 1 (PRV-1) and salmonid alphavirus (SAV) in skeletal muscle. However, these viruses were not consistently associated with lesioned areas, contrasting previous findings in Atlantic salmon. In conclusion, rainbow trout develop MFCs of a different character than farmed Atlantic salmon, and we speculate whether the observed pathological differences are contributing to their reduced occurrence in farmed rainbow trout.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"158 ","pages":"201-213"},"PeriodicalIF":1.1,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141455883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0