Diseases of aquatic organisms最新文献_第3页

Antimicrobial susceptibility of Peruvian Yersinia ruckeri isolates to oxytetracycline and florfenicol using MIC testing. 利用 MIC 测试法，测定秘鲁 ruckeri 耶尔森氏菌分离株对土霉素和氟苯尼考的抗菌敏感性。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-08-29 DOI: 10.3354/dao03810

Marco Medina-Morillo, Rute Irgang, Jefferson Yunis-Aguinaga, Ruben Avendaño-Herrera

{"title":"Antimicrobial susceptibility of Peruvian Yersinia ruckeri isolates to oxytetracycline and florfenicol using MIC testing.","authors":"Marco Medina-Morillo, Rute Irgang, Jefferson Yunis-Aguinaga, Ruben Avendaño-Herrera","doi":"10.3354/dao03810","DOIUrl":"https://doi.org/10.3354/dao03810","url":null,"abstract":"Yersiniosis, caused by Yersinia ruckeri, has become the most common disease in farmed rainbow trout Oncorhynchus mykiss in Peru, affecting Puno and Junín Regions, important aquaculture areas in the country. Florfenicol (FLO) and oxytetracycline (OXY) are the antimicrobials most frequently used to mitigate losses attributed to this pathogen. This study presents an analysis of the susceptibility patterns of 60 Y. ruckeri isolates (30 isolates each from Puno and Junín), including the type strain CECT 4319T and the strains CECT 955 and CECT 956, against FLO and OXY. Minimum inhibitory concentrations (MICs) were determined following the guideline for standard broth dilution method published by the Clinical and Laboratory Standards Institute. MIC results ranged from 4.0 to 8.0 µg ml-1 for FLO and 0.5 to 4.0 µg ml-1 for OXY. Normalized resistance interpretation (NRI) analysis identified epidemiological cut-off values of ≤16.0 µg ml-1 for FLO and ≤4.0 µg ml-1 for OXY. All Peruvian isolates, including the collection strains, were categorized as wild-type for both antimicrobials. Even though the number of Y. ruckeri isolates with MIC values of 8 µg ml-1 for FLO is more than double in Puno than in Junín (15 vs. 7 isolates), the NRI analysis showed the same epidemiological cutoff of 16 µg ml-1; while for OXY, it was 4.0 µg ml-1 for Puno and 2.0 µg ml-1 for Junín. This study establishes the basis for monitoring susceptibility to FLO and OXY in new Y. ruckeri isolates in Peruvian rainbow trout farming.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"159 ","pages":"127-131"},"PeriodicalIF":1.1,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal patterns in coral disease prevalences at Thudufushi Island, Maldives, 2010-2022. 马尔代夫 Thudufushi 岛珊瑚疾病流行的时间模式，2010-2022 年。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-08-29 DOI: 10.3354/dao03807

Chiara Bises, Jacopo Gobbato, Niccolò Lainati, Inga Dehnert, Federica Siena, Davide Seveso, Enrico Montalbetti, Yohan Didier Louis, Simone Montano

{"title":"Temporal patterns in coral disease prevalences at Thudufushi Island, Maldives, 2010-2022.","authors":"Chiara Bises, Jacopo Gobbato, Niccolò Lainati, Inga Dehnert, Federica Siena, Davide Seveso, Enrico Montalbetti, Yohan Didier Louis, Simone Montano","doi":"10.3354/dao03807","DOIUrl":"https://doi.org/10.3354/dao03807","url":null,"abstract":"Coral reefs are lately suffering a fast decline in biodiversity due to the coupled effect of climate change and disease outbreaks, which in recent decades have been reported with higher frequency and shorter intervals. Limited studies have been conducted on coral diseases in the Maldives resulting in the impossibility of assessing the temporal trend in their dynamics. In this context, we evaluated the change in the distribution, prevalence, and host range of 4 diseases, namely black band disease (BBD), brown band disease (BrB), skeletal eroding band (SEB) and white syndrome (WS), in the reef system around Thudufushi Island after an interval of 12 yr since the last assessment. In this period, the overall disease prevalence increased, except for BrB, with SEB showing the most severe increase in 2022 in comparison to 2010. The overall average prevalence of coral diseases is approximately 2%, indicating an increase of about 0.7% since 2010. Diseased coral colonies were found in all the investigated sites, with the east site being the most affected and SEB emerging as the most prevalent disease across all the investigated sites. The affected colonies belong to 13 genera, with Psammocora genus showing the highest overall mean disease prevalence. This study depicted a basic temporal trend in disease prevalence that confirms an increase in coral diseases in the region and calls for a dedicated national monitoring protocol to better understand and predict future coral disease dynamics at regional scales.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"159 ","pages":"133-142"},"PeriodicalIF":1.1,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and antifungal sensitivity of Fusarium species isolated from piscine hosts. 从鱼类宿主中分离出的镰刀菌种的鉴定和抗真菌敏感性。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-08-29 DOI: 10.3354/dao03809

Victoria Chanu Khangembam, Dimpal Thakuria, Ritesh Shantilal Tandel, Vinita Pant, Nityanand Pandey, Pramod Kumar Pandey

{"title":"Identification and antifungal sensitivity of Fusarium species isolated from piscine hosts.","authors":"Victoria Chanu Khangembam, Dimpal Thakuria, Ritesh Shantilal Tandel, Vinita Pant, Nityanand Pandey, Pramod Kumar Pandey","doi":"10.3354/dao03809","DOIUrl":"https://doi.org/10.3354/dao03809","url":null,"abstract":"Fusarium is a huge genus of filamentous fungi that has the potential to cause emerging diseases. Members of this genus can cause infections in plants, animals and humans. Here, we report the isolation of F. oxysporum and F. equiseti from 2 important fish species, Oncorhynchus mykiss (rainbow trout) and Tor putitora (golden mahseer), respectively. F. oxysporum has emerged as a significant fungal pathogen causing infection in many fish. However, F. equiseti has been isolated mainly from plants. As far as the available literatures are concerned, this is the first report on the isolation of F. oxysporum and F. equiseti from these hosts. The isolates were identified based on growth morphology and microscopic observation. F. oxysporum produced violet pigmentation on potato dextrose agar, while F. equiseti had yellow colouration. F. oxysporum produced 1- to 2-celled microconidia along with straight or curved macroconidia having 3 to 4 septa. F. equiseti produced abundant macroconidia with 4 or more septa. Species were further confirmed based on the nucleotide sequences of the internal transcribed spacer region. In a molecular phylogeny analysis, F. oxysporum and F. equiseti formed 2 different clades. In an antifungal sensitivity assay, F. oxysporum was found to be susceptible to clotrimazole with a minimum inhibitory concentration of 1.0 µg ml-1, whereas F. equiseti was susceptible to clotrimazole, ketoconazole and fluconazole. Overall, the main findings of this study are the infection of new hosts by Fusarium species and the limited activity of many antifungal drugs against these pathogens.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"159 ","pages":"117-126"},"PeriodicalIF":1.1,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shewanella putrefaciens as an emerging pathogen of hepatopancreas necrosis disease in Chinese mitten crab Eriocheir sinensis. 中华绒螯蟹肝胰腺坏死病的新病原体--普氏雪旺菌。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-08-29 DOI: 10.3354/dao03811

Jie Liu, Chenhao Teng, Xurui Zheng, La Xu, Haipeng Cao, Chunlei Gai

{"title":"Shewanella putrefaciens as an emerging pathogen of hepatopancreas necrosis disease in Chinese mitten crab Eriocheir sinensis.","authors":"Jie Liu, Chenhao Teng, Xurui Zheng, La Xu, Haipeng Cao, Chunlei Gai","doi":"10.3354/dao03811","DOIUrl":"https://doi.org/10.3354/dao03811","url":null,"abstract":"Shewanella putrefaciens has been recognized as an emerging important pathogen in aquaculture. However, scarce information is available on the characterization and microbial control of S. putrefaciens as a causal agent of hepatopancreas necrosis disease in Chinese mitten crab Eriocheir sinensis. In this study, a multi-resistant S. putrefaciens isolate (DZ-A) was identified as a causal pathogen of hepatopancreas necrosis disease in Chinese mitten crabs. It showed a lethal dose (LD50) of 2.20 × 105 CFU ml-1 in Chinese mitten crabs, and multiple resistance to aminoglycoside, chloramphenicol, macrolide, penicillin, peptide, and tetracycline antimicrobials. In addition, Bdellovibrio powder exhibited a significant antibacterial effect against the pathogenic S. putrefaciens, and conferred significant protection to challenged Chinese mitten crabs with relative percentage survivals of 80.00% to 93.33% via significant improvement in their immune response and antioxidant capability. The findings of this study provide valuable insights into the phenotypic characterization and biological control of pathogenic S. putrefaciens in Chinese mitten crabs.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"159 ","pages":"143-152"},"PeriodicalIF":1.1,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between skin and body condition in three species of baleen whales. 三种须鲸的皮肤与身体状况之间的关系。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-08-15 DOI: 10.3354/dao03808

Joyce Neves, Séverine Methion, Bruno Díaz López

{"title":"Relationship between skin and body condition in three species of baleen whales.","authors":"Joyce Neves, Séverine Methion, Bruno Díaz López","doi":"10.3354/dao03808","DOIUrl":"https://doi.org/10.3354/dao03808","url":null,"abstract":"The assessment of free-ranging cetacean health through the study of skin conditions using photographs has gained prominence in recent years. However, little attention has been given to the relationships between cetacean skin conditions, species, and body condition. To explore this relationship among baleen whale species along the northwestern coast of Spain, we employed a non-invasive method involving photograph analysis. In this study, we examined skin conditions (including injuries, epizoites and ectoparasites, pigmentation disorders, skin lesions, and anatomical malformations) and body condition (overall physical contours and form, as an indicator of nutritional status and health) in 3 species of whales (blue, fin, and minke whales). This methodology facilitated the identification of 29 subcategories of distinct skin conditions and an assessment of body condition over a 5 yr period (2017 to 2021). In our study, we present evidence linking hypopigmentation, protruding pieces of tissue, and tattoo-like lesions to 'Poor' body condition in the 3 baleen whale species. Fin whales exhibited a higher susceptibility to mottling (prevalence = 17.7%), while blue whales were more prone to starbursts (prevalence = 90.5%). Additionally, we found a significant relationship between skin condition diversity and individual body condition. Our findings contribute valuable information to the broader understanding of the health status of baleen whales. Further investigations are necessary to delve into the etiology of the documented skin conditions and their potential implications for individual survival. This study serves as a foundation for ongoing research aimed at advancing our comprehension of these findings.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"159 ","pages":"99-115"},"PeriodicalIF":1.1,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141981959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Francisella sciaenopsi sp. nov. isolated from diseased red drum Sciaenops ocellatus in Florida, USA. Francisella sciaenopsi sp.

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-08-15 DOI: 10.3354/dao03803

Miku Kawahara, Theresa T Cody, Roy P E Yanong, Eileen Henderson, Zeinab Yazdi, Esteban Soto

{"title":"Francisella sciaenopsi sp. nov. isolated from diseased red drum Sciaenops ocellatus in Florida, USA.","authors":"Miku Kawahara, Theresa T Cody, Roy P E Yanong, Eileen Henderson, Zeinab Yazdi, Esteban Soto","doi":"10.3354/dao03803","DOIUrl":"https://doi.org/10.3354/dao03803","url":null,"abstract":"Piscine francisellosis is one of the most important bacterial diseases affecting various fish species worldwide. Francisella orientalis, F. noatunensis, and F. salimarina (F. marina) have been reported as etiological agents of disease in fish. A Francisella sp. was isolated from several diseased red drum Sciaenops ocellatus experiencing morbidity in Florida, USA, in 2008. In this study, molecular and phenotypic characterization of the recovered isolate was conducted. Phenotypically, the isolate showed a biochemical reaction profile distinct from that of F. orientalis and F. salimarina. Although the 16S rRNA sequence of this isolate shared 99.61% identity to the type strain of F. philomiragia O#319LT, whole genome analysis (average nucleotide identity <95%; digital DNA-DNA hybridization <70%) and a multilocus sequence analysis of 8 concatenated housekeeping genes in comparison with other Francisella spp. indicated that this isolate was a novel Francisella species, more closely related to F. orientalis. Immersion, intracoelomic injection, and co-habitation challenges using a Nile tilapia Oreochromis niloticus fingerling model of infection were done to investigate virulence in a piscine model. Variably pigmented granulomas and pigmented macrophage aggregates were observed in the kidneys and spleens of the challenged fish, but no mortality was recorded during the 15 d challenge period, suggesting that this novel Francisella sp. might be an opportunistic pathogen of fish. Based on the phenotypic and genotypic differences from other Francisella spp. observed in this study, we propose the name Francisella sciaenopsi sp. nov. for this novel isolate.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"159 ","pages":"79-89"},"PeriodicalIF":1.1,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141981958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First case of ichthyophonosis in farmed rainbow trout Oncorhynchus mykiss in Serbia. 塞尔维亚首例养殖虹鳟鱼鱼鳞病病例。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-08-15 DOI: 10.3354/dao03804

Vladimir Radosavljevic, Oliver Radanovic, Dimitrije Glišic, Nemanja Zdravkovic, Jelena Maksimovic-Zoric, Ksenija Nesic, Bozidar Savic, Bozidar Raškovic

引用次数: 0

RNA interference targeting WSSV ribonucleotide reductase 2 provides long-term protection against infection in Litopenaeus vannamei. 以 WSSV 核糖核苷酸还原酶 2 为靶点的 RNA 干扰可提供长期保护，防止万年青感染。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-08-15 DOI: 10.3354/dao03805

Soon Joo Hong, Ki Hong Kim

{"title":"RNA interference targeting WSSV ribonucleotide reductase 2 provides long-term protection against infection in Litopenaeus vannamei.","authors":"Soon Joo Hong, Ki Hong Kim","doi":"10.3354/dao03805","DOIUrl":"10.3354/dao03805","url":null,"abstract":"Many studies have demonstrated that long double-stranded RNAs (dsRNAs) targeting essential genes of white spot syndrome virus (WSSV) can induce a sequence-specific antiviral RNA interference (RNAi) response in shrimp, thereby offering protection against WSSV infection. However, further experimental data on the required dose of dsRNAs and the duration of protection from a single administration are necessary to establish RNAi-mediated methods as effective and practical antiviral measures. In this study, we evaluated the protective efficacy and the duration of protection provided by a single administration of various doses of long dsRNA targeting WSSV ribonucleotide reductase 2 (rr2) in white-leg shrimp Litopenaeus vannamei. The protective efficacy of long dsRNA targeting WSSV rr2 was not diminished by the reduction of the dose to 100 ng g-1 of body weight, suggesting that a relatively low dose can effectively induce an RNAi response in shrimp. Furthermore, shrimp were well-protected against WSSV challenges for up to 4 wk post-administration of the rr2-targeting long dsRNA, although the protective effect almost disappeared at 6 wk post-administration. These results suggest that long dsRNAs can provide protection against WSSV for at least 1 mo, and monthly administration of long dsRNAs could serve as a long-term protective strategy for shrimp against WSSV.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"159 ","pages":"71-78"},"PeriodicalIF":1.1,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141981960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of buccal cavity parasite Ceratothoa carinata (Isopoda, Cymothoidae) on the condition and reproduction of its host fish Japanese scad Decapterus maruadsi. 颊腔寄生虫 Ceratothoa carinata（等足目，鲤科）对宿主鱼日本鳞鯵的状态和繁殖的影响。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-08-15 DOI: 10.3354/dao03806

Reina Nakano, Yuzumi Okumura, Hiroki Hata

引用次数: 0

Regeneration of tunic cuticle is suppressed in edible ascidian Halocynthia roretzi contracting soft tunic syndrome. 食用腹足纲 Halocynthia roretzi 感染软鳞茎皮综合征后，鳞茎皮的再生受到抑制。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-08-01 DOI: 10.3354/dao03801

Kei Nakayama, Yumiko Obayashi, Leo Munechika, Shin-Ichi Kitamura, Tetsuya Yanagida, Miho Honjo, Shoko Murakami, Euichi Hirose

{"title":"Regeneration of tunic cuticle is suppressed in edible ascidian Halocynthia roretzi contracting soft tunic syndrome.","authors":"Kei Nakayama, Yumiko Obayashi, Leo Munechika, Shin-Ichi Kitamura, Tetsuya Yanagida, Miho Honjo, Shoko Murakami, Euichi Hirose","doi":"10.3354/dao03801","DOIUrl":"https://doi.org/10.3354/dao03801","url":null,"abstract":"Soft tunic syndrome is an infectious disease caused by the flagellate Azumiobodo hoyamushi, which severely damages the aquaculture of the edible ascidian Halocynthia roretzi. Tunic is a cellulosic extracellular matrix entirely covering the body in ascidians and other tunicates, and its dense cuticle layer covers the tunic surface as a physical barrier against microorganisms. When the tunic of intact H. roretzi individuals was cut into strips, electron-dense fibers (DFs) appeared on the cut surface of the tunic matrix and aggregated to regenerate a new cuticular layer in seawater within a few days. DF formation was partially or completely inhibited in individuals with soft tunic syndrome, and DF formation was also inhibited by the presence of some proteases, indicating the involvement of proteolysis in the process of tunic softening as well as cuticle regeneration. Using pure cultures of the causative flagellate A. hoyamushi, the expression of protease genes and secretion of some proteases were confirmed by RNA-seq analysis and a 4-methylcoumaryl-7-amide substrate assay. Some of these proteases may degrade proteins in the tunic matrix. These findings suggest that the proteases of A. hoyamushi is the key to understanding the mechanisms of cuticular regeneration inhibition and tunic softening.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"159 ","pages":"37-48"},"PeriodicalIF":1.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0