Diseases of aquatic organisms最新文献_第3页

Saprolegnia species isolated from rainbow trout: molecular identification, specific PCR and drug sensitivity. 虹鳟病原菌的分子鉴定、特异性PCR及药敏分析。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2026-06-04 DOI: 10.3354/dao03917

Victoria Chanu Khangembam, Dimpal Thakuria, Garima Garima, Vinita Pant, Kishor Kunal

{"title":"Saprolegnia species isolated from rainbow trout: molecular identification, specific PCR and drug sensitivity.","authors":"Victoria Chanu Khangembam, Dimpal Thakuria, Garima Garima, Vinita Pant, Kishor Kunal","doi":"10.3354/dao03917","DOIUrl":"10.3354/dao03917","url":null,"abstract":"Saprolegniosis is a common infectious disease caused by members of the genus Saprolegnia. This genus contains several species, some of which can cause severe infection in vulnerable fish such as rainbow trout Oncorhynchus mykiss. This study reports the isolation and identification of 4 Saprolegnia species, namely S. parasitica, S. diclina, S. aenigmatica and S. asterophora, from a rainbow trout farm. The isolates were identified by amplification and sequencing of the internal transcribed spacer region. Among the isolates, S. parasitica and S. diclina were more common. S. diclina was found only in eggs and alevins, whereas S. parasitica was observed in different life stages of rainbow trout from fry to adult. Using the isolates, a species-specific PCR for the molecular identification of S. parasitica was optimised; the PCR protocol had a limit of detection of 0.025 ng template in a 20 µl reaction. Sensitivity of S. parasitica and S. diclina to boric acid and hydrogen peroxide was also determined; this assay also included another important Saprolegnia species (S. australis isolated from rainbow trout from a different rearing facility) to evaluate the comparative response among different species. S. parasitica and S. diclina showed similar sensitivity to boric acid, whereas S. diclina showed the highest sensitivity to hydrogen peroxide followed by S. parasitica and S. australis. This highlights the importance of accurate species identification for choosing suitable control measures and the judicious use of anti-oomycete drugs.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"166 ","pages":"1-11"},"PeriodicalIF":1.2,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148155815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viability of probiotic C6-6 in fish feed and ability to inhibit columnaris-causing bacteria in rainbow trout Oncorhynchus mykiss. 鱼饲料中益生菌C6-6的活力及对虹鳟柱状致病菌的抑制能力。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2026-05-21 DOI: 10.3354/dao03916

Ryan Maxwell, Jie Ma, Benjamin R LaFrentz, Shelby Swearingen, Kenneth Cain

{"title":"Viability of probiotic C6-6 in fish feed and ability to inhibit columnaris-causing bacteria in rainbow trout Oncorhynchus mykiss.","authors":"Ryan Maxwell, Jie Ma, Benjamin R LaFrentz, Shelby Swearingen, Kenneth Cain","doi":"10.3354/dao03916","DOIUrl":"10.3354/dao03916","url":null,"abstract":"Probiotics are increasingly recognized as sustainable alternatives to antibiotics in aquaculture. This study evaluated the viability and disease-mitigating potential of the candidate probiotic Lelliotia amnigena strain C6-6 in rainbow trout Oncorhynchus mykiss. Viability was assessed under varying storage temperatures (-15, 4, and 17°C), time intervals (up to 24 wk), and feed preparation methods. Results showed that colder storage, particularly at -15°C, preserved higher concentrations of C6-6, while viability rapidly declined at 17°C. The viability of C6-6 over time was enhanced in feeds prepared without oil. In vitro assays demonstrated that C6-6 inhibited the growth of 3 species of columnaris-causing bacteria, namely Flavobacterium columnare, F. covae, and F. davisii. In vivo, trout fed C6-6 showed significantly reduced mortality (14%) compared to controls (26%) following F. columnare challenge, and C6-6 was reisolated from the gastrointestinal tract of treated fish. These findings highlight C6-6 as a promising probiotic with strong antagonistic activity and stable viability under proper storage, supporting its potential use in disease management strategies for coldwater aquaculture.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"166 ","pages":"1-11"},"PeriodicalIF":1.2,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147980856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production and evaluation of a rabbit polyclonal antibody specific to the Aeromonas hydrophila outer membrane protein Omp38. 兔嗜水气单胞菌外膜蛋白Omp38多克隆抗体的制备及评价。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2026-05-07 DOI: 10.3354/dao03914

Tri Nguyen Do, Binh Nhu Nguyen, Tran Thi Tran, Trinh Thi Nguyen

引用次数: 0

Detection of Anguillicoloides crassus (Nematoda) and a survey of endohelminths of American eels in Georgia, USA. 美国乔治亚州美洲鳗类线虫（线虫）的检测及内蠕虫的调查。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2026-05-07 DOI: 10.3354/dao03915

Maggie A Young, Makala E Fabrizio, Vasyl V Tkach, Jim Page, Amory Cook, J Mike Kinsella, Florian B Reyda, Anindo Choudhury, Brandon W Baker, Tyler J Achatz

{"title":"Detection of Anguillicoloides crassus (Nematoda) and a survey of endohelminths of American eels in Georgia, USA.","authors":"Maggie A Young, Makala E Fabrizio, Vasyl V Tkach, Jim Page, Amory Cook, J Mike Kinsella, Florian B Reyda, Anindo Choudhury, Brandon W Baker, Tyler J Achatz","doi":"10.3354/dao03915","DOIUrl":"10.3354/dao03915","url":null,"abstract":"The American eel Anguilla rostrata is a catadromous fish with a unique life history; however, parasite surveys of these eels in the southeastern USA are limited. In this study, we surveyed the parasite fauna of 98 American eels collected from the Altamaha and Ocmulgee rivers in Georgia between January 2024 and August 2025. Eels were examined for external and internal parasites. The invasive, pathogenic swim bladder nematode Anguillicoloides crassus was detected in 62 of 98 eels (63.2% prevalence), with all infections occurring in the Altamaha River population. Infected eels harbored up to 21 nematodes, although most infections consisted of fewer than 5 individuals. No clear seasonal trends of infection were observed. The prevalence and mean intensity of A. crassus infections observed in Georgia were higher than those reported for most other regions along the US east coast. This study provides the first published record of A. crassus from Georgia and expands the known parasite diversity of American eels within the state. Our data also demonstrates the presence of 2 trematode genera, 3 cestode genera, 6 nematode genera, 3 acanthocephalan genera, and 1 arthropod genus. Many of these represent the first reports from eels in the state of Georgia.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"166 ","pages":"1-5"},"PeriodicalIF":1.2,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact and distribution of Perkinsus olsenii in cultivated Mediterranean mussel Mytilus galloprovincialis from the southern coast of Campania (Italy). 意大利坎帕尼亚南部海岸栽培的地中海贻贝（Mytilus galloprovincialis）中桃金螺的影响和分布。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2026-05-07 DOI: 10.3354/dao03913

Francesca Carella, Lorenza D'Angelo, Laura Troise, Emanuela Di Lecce, Gianluigi Paduano, Maria Gabriella Lucibelli, Fabio Di Nocera

{"title":"Impact and distribution of Perkinsus olsenii in cultivated Mediterranean mussel Mytilus galloprovincialis from the southern coast of Campania (Italy).","authors":"Francesca Carella, Lorenza D'Angelo, Laura Troise, Emanuela Di Lecce, Gianluigi Paduano, Maria Gabriella Lucibelli, Fabio Di Nocera","doi":"10.3354/dao03913","DOIUrl":"10.3354/dao03913","url":null,"abstract":"Following a previous description from the Campania region (southern Italy), a survey for perkinsosis was performed at 12 mussel farms along the Italian coast during the period October 2022 to July 2023. The prevalence of Perkinsus parasites was estimated by PCR using genus-specific primers. Seven farms tested positive and sequencing was performed to identify the Perkinsus species. The parasite Perkinsus olsenii was detected in the farm samples with a BLAST identity of 97-100%. One of the affected farms was also monitored for the period of July 2022-June 2023 using histopathology, Ray's fluid thioglycollate culture method, and quantitative PCR. The sensitivity of each of the techniques was also determined. Trophozoite stages were observed mostly aggregated in the connective tissue of the digestive tract and gonad/mantle, and to a lesser extent in the foot and kidney, producing an encapsulated inflammatory response that occupied most of the tissues in some samples. The condition index of affected individuals was lower than in healthy ones (p < 0.05) and larger animals (5.8-6 cm) were more often infected (p < 0.05). This study provides information regarding P. olsenii pathogen infections in M. galloprovincialis in the Campania region, its seasonal variations, and host-parasite interactions over a 1 year period.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"166 ","pages":"1-15"},"PeriodicalIF":1.2,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infection dynamics of Batrachochytrium dendrobatidis in subtropical amphibian communities. 亚热带两栖动物群落中水蛭壶菌的感染动态。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2026-05-07 DOI: 10.3354/dao03911

Camila F Moser, Wesley J Neely, Leigha M Stahl, Laura K Schuck, Henrique Schipanski, C Guilherme Becker, Pedro Peloso

{"title":"Infection dynamics of Batrachochytrium dendrobatidis in subtropical amphibian communities.","authors":"Camila F Moser, Wesley J Neely, Leigha M Stahl, Laura K Schuck, Henrique Schipanski, C Guilherme Becker, Pedro Peloso","doi":"10.3354/dao03911","DOIUrl":"10.3354/dao03911","url":null,"abstract":"Batrachochytrium dendrobatidis (Bd) is a pathogenic fungus causing population declines and extinctions of amphibians globally. Anthropogenic habitat alteration can directly and indirectly influence host-pathogen interactions by modifying wetland microclimates, physicochemical profiles, and amphibian communities. To test the combined effects of host-specific traits and environmental factors on Bd infection dynamics, we sampled 581 amphibians from 12 species in Tuscaloosa County, Alabama, USA. We tested the influence of host traits (body size and ecological behavioral habit) and environmental factors (habitat disturbance and water temperature) on Bd prevalence and infection loads, while body size was included in the models only as a covariate to control for potential effects on infection parameters. Bd was detected in 127 individuals (22% community prevalence) across 6 frog and 2 salamander species. Aquatic amphibians showed higher Bd prevalence and loads than terrestrial and arboreal species, with no significant differences between the latter 2 groups. Higher water temperatures were associated with lower Bd prevalence and loads, while body size and habitat disturbance were not important Bd predictors. These results emphasize the influence of host traits and environmental factors on Bd dynamics, expanding our understanding of amphibian host-pathogen interactions in an understudied region.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"166 ","pages":"1-12"},"PeriodicalIF":1.2,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147834980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Haplosporidium pinnae infection confirmed in Pinna nobilis populations in the Sea of Marmara. 在马尔马拉海的大耳蜗种群中证实有耳单孢子虫感染。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2026-04-23 DOI: 10.3354/dao03912

Uğur Karadurmuş, Emre Turgay, Mert Gürkan, İbrahim Ender Künili, Selin Ertürk Gürkan, Mustafa Sarı

{"title":"Haplosporidium pinnae infection confirmed in Pinna nobilis populations in the Sea of Marmara.","authors":"Uğur Karadurmuş, Emre Turgay, Mert Gürkan, İbrahim Ender Künili, Selin Ertürk Gürkan, Mustafa Sarı","doi":"10.3354/dao03912","DOIUrl":"10.3354/dao03912","url":null,"abstract":"Fan mussels Pinna nobilis across the Mediterranean Sea have been severely impacted by a widespread mass mortality event, largely attributed to Haplosporidium pinnae. While the Sea of Marmara (SoM) has historically served as a refuge for this Critically Endangered species, recent findings suggest the emergence of various pathogens, including haplosporidian parasites, in SoM populations. This study presents the first confirmed case of H. pinnae infection in fan mussels from the southern SoM, with a focus on species-level identification, and contributes to growing concerns about the potential spread of this pathogen into previously unaffected regions. On 23 November 2024, 5 live fan mussels were collected from depths of 2.3-6.5 m in the southern SoM. Histopathological examination revealed structural alterations in the mantle tissue and the presence of plasmodial stages of H. pinnae. Molecular analyses further confirmed the presence of H. pinnae in several samples, exhibiting 100% sequence similarity with isolates from other Mediterranean regions. This study provides essential evidence of infection in the SoM and underscores the need for continued monitoring and conservation efforts for fan mussel populations in the region, especially as pathogens continue to spread across the Mediterranean Sea. The results represent a significant conservation alarm and highlight the urgent need for continued pathogen surveillance, early warning strategies, and robust management interventions in one of the last strongholds of this keystone species.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"166 ","pages":"1-6"},"PeriodicalIF":1.2,"publicationDate":"2026-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147765493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging pathogen Chryseobacterium sp. isolated from diseased goldfish Carassius auratus: antimicrobial susceptibility and pathology. 从患病金鱼鲫鱼中分离的新发病原体黄杆菌：抗菌敏感性和病理学。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2026-04-09 DOI: 10.3354/dao03910

Rajamani Srinath, Arun Sudhagar, Arathi Dharmaratnam, Sagar Gorakh Satkar, Valaparambil Saidmuhammed Basheer

{"title":"Emerging pathogen Chryseobacterium sp. isolated from diseased goldfish Carassius auratus: antimicrobial susceptibility and pathology.","authors":"Rajamani Srinath, Arun Sudhagar, Arathi Dharmaratnam, Sagar Gorakh Satkar, Valaparambil Saidmuhammed Basheer","doi":"10.3354/dao03910","DOIUrl":"10.3354/dao03910","url":null,"abstract":"Chryseobacterium sp., belonging to the family Weeksellaceae, is an emerging fish pathogen that is opportunistic in nature. In this study, we investigated a clinically infected goldfish Carassius auratus from a fish farm during a disease outbreak. The bacterial colonies were isolated from the kidneys of infected goldfish. Following a 24 h incubation at 28°C, the colonies on nutrient agar exhibited yellow pigmentation and were identified as Gram-negative, rod-shaped, catalase-positive, and oxidase-positive. A total of 35 antibiotics belonging to different classes were used to determine the antibiotic susceptibility pattern of the isolated bacteria. Amplification and sequence analysis of the 16S rRNA gene identified the microorganism as Chryseobacterium sp., sharing 99.3% sequence similarity. The pathogenicity of this bacterial pathogen was experimentally confirmed in goldfish by a challenge experiment, and LD50 was determined as 7.46 × 107 CFU ml-1. Histopathological analysis of the infected fish revealed degeneration of renal tubule epithelium and melano-macrophagic aggregates with hyperaemic condition in the kidney. Similarly, infected gills exhibit primary and secondary lamellar hyperplasia, with lamellar oedema, necrosis and fusion of secondary lamella. This study underscores the virulence of Chryseobacterium sp. as a significant opportunistic pathogen in goldfish, highlighting the need for vigilant monitoring and intervention strategies to prevent outbreaks.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"166 ","pages":"1-12"},"PeriodicalIF":1.2,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147638324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emaciation and marine debris contribute to sperm whale Physeter macrocephalus strandings in southeastern USA: comparing 4 cases from 2020-2022. 美国东南部的抹香鲸phseter macrocephalus搁浅：比较2020-2022年的4个案例。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2026-04-09 DOI: 10.3354/dao03909

Jennifer C G Bloodgood, Emily J Cramer, Carl S Cloyed, Michelle D Staudinger, Mackenzie L Russell, Ruth H Carmichael, Kathleen M Colegrove, David S Rotstein, Wayne E McFee, Nicole I Stacy, Lauren E Albrittain, Wendy B Marks, Jillian R Schwartz, Denise Boyd

{"title":"Emaciation and marine debris contribute to sperm whale Physeter macrocephalus strandings in southeastern USA: comparing 4 cases from 2020-2022.","authors":"Jennifer C G Bloodgood, Emily J Cramer, Carl S Cloyed, Michelle D Staudinger, Mackenzie L Russell, Ruth H Carmichael, Kathleen M Colegrove, David S Rotstein, Wayne E McFee, Nicole I Stacy, Lauren E Albrittain, Wendy B Marks, Jillian R Schwartz, Denise Boyd","doi":"10.3354/dao03909","DOIUrl":"10.3354/dao03909","url":null,"abstract":"Large whale strandings along the east coast of the USA have increased in recent years, resulting in unusual mortality event (UME) declarations for several species. Endangered sperm whales Physeter macrocephalus have not been included in a UME; however, recent strandings have shown commonalities that warrant investigation. We analyzed 4 live-stranded sperm whales ('case' whales) in waters of the southeastern USA from 2020-2022 to determine if common factors contributed to death and to inform future management decisions. Thorough investigations were performed, including necropsy, histopathology, and infectious disease and biotoxin testing. Major findings were related to emaciation; there was no evidence of infectious disease or significant toxin exposure. Two of the whales had ingested fishing gear and other marine debris that may have contributed to emaciation. Prey identification and stable isotope analysis (SIA) of these whales combined with SIA of 4 additional sperm whales ('reference' whales) provided further life history data for this species. Prey consumed were primarily cephalopods from families consistent with known sperm whale diets in other parts of the world, and SIA suggested that case whales had similar foraging habitats and trophic levels compared to reference whales. The common finding of emaciation in case whales despite typical prey consumption emphasizes the need for continued research on factors that may influence foraging efficiency or prey availability and quality in this region. Furthermore, finding ingested fishing gear in 2 whales supports continued efforts to monitor and mitigate human impacts to support long-term survival and recovery of sperm whale populations.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"166 ","pages":"1-19"},"PeriodicalIF":1.2,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147638337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intestinal entamoebiasis in a captive colony of Anderson's crocodile newts Echinotriton andersoni. 圈养安徒生鳄蝾螈群肠道内阿米巴病的研究。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2026-03-26 DOI: 10.3354/dao03908

Varvara Semenova, Nicholas A Buscaglia, Robert J Ossiboff

{"title":"Intestinal entamoebiasis in a captive colony of Anderson's crocodile newts Echinotriton andersoni.","authors":"Varvara Semenova, Nicholas A Buscaglia, Robert J Ossiboff","doi":"10.3354/dao03908","DOIUrl":"10.3354/dao03908","url":null,"abstract":"Entamoeba protozoa are known to cause enteric and sometimes systemic disease in both domestic and non-domestic animals. In amphibians, Entamoeba spp. are primarily documented as incidental gastrointestinal inhabitants, with rare reports of clinically significant entamoebiasis. In the last decade, mortality outbreaks caused by a recently described protozoan, Entamoeba sp. CT1, have been documented in free-ranging anurans in Australia and managed anurans in the USA associated with gastroenteritis and substantial morbidity and mortality. In 2023, a small population of Anderson's crocodile newts Echinotriton andersoni in managed care at a zoological institution experienced a disease outbreak resulting in the death or euthanasia of 4/13 (31%) newts over an approximately 1 mo period. Clinical signs included dehydration, poor skin quality, and lethargy. Postmortem examination of 3 newts revealed marked, proliferative, and necrotizing inflammation of the intestinal tract, urinary bladder, and cloaca with intramucosal amoebic protozoa. The gonadal ducts (2/3 newts) were also variably affected. Entamoeba PCR and Sanger sequencing of formalin-fixed paraffin-embedded intestinal tissue from all examined newts amplified DNA with 100% sequence identity to the recently identified Entamoeba sp. CT1. However, unlike reports of Entamoeba sp. CT1 in anurans, lesions in the crocodile newts were not restricted to the gastrointestinal tract. This is the first report of Entamoeba sp. CT1 causing significant clinical disease in caudates, emphasizing its importance as an amphibian pathogen with the potential to cause disease in managed and free-ranging amphibian populations.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"165 ","pages":"1-6"},"PeriodicalIF":1.2,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147510500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0