Tissue tropism and molecular data of Myxobolus ellipsoides ex S. cephalus (Myxozoa: Myxobolidae) infecting European chub Squalius cephalus.

IF 1.1 4区 农林科学 Q3 FISHERIES
Eva Lewisch, Josef Harl, Hans-Peter Fuehrer, Licha N Wortha, Kurt Pinter, Mansour El-Matbouli
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Abstract

In the River Traun in Austria, diseased European chub Squalius cephalus were observed for several years. In 2019, an investigation of the condition revealed the presence of several myxozoan species in different tissues, without evidence of other pathogens. The most prevalent and abundant myxozoan parasite in the different organs was Myxobolus ellipsoides ex S. cephalus, a parasite formerly only reported to infect the fin of European chub. To further investigate tissue tropism and molecular data of this parasite, samples from 11 different organs of 13 European chub were collected 1 yr later and examined with various methods. Myxospore morphology was assessed by microscopy and compared to the literature. A specific PCR protocol targeting the 18S rRNA gene of M. ellipsoides ex S. cephalus and subsequent sequence analyses detected 11 different 18S variants clustering into 2 groups. To differentiate M. ellipsoides ex S. cephalus unambiguously from other myxozoan parasites in the tissues, histological methods and in situ hybridization with a species-specific probe targeting the 18S rRNA of the parasite were applied. DNA of M. ellipsoides ex S. cephalus could be detected by PCR in each of the examined fish in at least 2 of the sampled organs, but not in any blood sample. In 2 fish, M. ellipsoides ex S. cephalus myxospores were detected in plasmodia in the kidney. Our findings present new data regarding tissue tropism and molecular diversity of M. ellipsoides ex S. cephalus in European chub and provide a basis for further studies investigating possible health impacts by this parasite.

欧洲头角鲨感染椭圆粘虫的组织趋向性和分子特征。
在奥地利的特劳恩河,人们观察到患病的欧洲头鲨已经好几年了。2019年,对这种疾病的一项调查显示,在不同组织中存在几种黏液动物,但没有其他病原体的证据。不同器官中最常见和最丰富的黏液虫是以前只报道感染欧洲鲟鱼鳍的粘虫。为了进一步研究该寄生虫的组织趋向性和分子数据,我们在1年后采集了13只欧洲俱乐部11个不同器官的样本,并采用各种方法进行了检测。用显微镜观察粘孢子的形态,并与文献进行比较。利用特异的PCR方法检测出了11种不同的18S rRNA基因,可分为2类。为了在组织中明确区分M. ellipsoides ex S. cephalus与其他黏液寄生虫,采用组织学方法和针对寄生虫18S rRNA的物种特异性探针原位杂交。在每条被检鱼的至少2个器官中均可检测到非头状椭球绦虫的DNA,但在所有血液样本中均未检测到。在2条鱼的肾内疟原虫中检出椭圆形m.s cephalus黏液孢子。本研究结果提供了有关欧洲球虫头螺外椭球绦虫组织趋向性和分子多样性的新数据,为进一步研究该寄生虫可能对健康的影响提供了基础。
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来源期刊
Diseases of aquatic organisms
Diseases of aquatic organisms 农林科学-兽医学
CiteScore
3.10
自引率
0.00%
发文量
53
审稿时长
8-16 weeks
期刊介绍: DAO publishes Research Articles, Reviews, and Notes, as well as Comments/Reply Comments (for details see DAO 48:161), Theme Sections and Opinion Pieces. For details consult the Guidelines for Authors. Papers may cover all forms of life - animals, plants and microorganisms - in marine, limnetic and brackish habitats. DAO''s scope includes any research focusing on diseases in aquatic organisms, specifically: -Diseases caused by coexisting organisms, e.g. viruses, bacteria, fungi, protistans, metazoans; characterization of pathogens -Diseases caused by abiotic factors (critical intensities of environmental properties, including pollution)- Diseases due to internal circumstances (innate, idiopathic, genetic)- Diseases due to proliferative disorders (neoplasms)- Disease diagnosis, treatment and prevention- Molecular aspects of diseases- Nutritional disorders- Stress and physical injuries- Epidemiology/epizootiology- Parasitology- Toxicology- Diseases of aquatic organisms affecting human health and well-being (with the focus on the aquatic organism)- Diseases as indicators of humanity''s detrimental impact on nature- Genomics, proteomics and metabolomics of disease- Immunology and disease prevention- Animal welfare- Zoonosis
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