Doklady Akademii nauk最新文献_第5页

Молекулярно-генетические механизмы регуляции дигидрофлавонол редуктазы и транскрипционного фактора Hy5 экзогенной 5-аминолевулиновой кислотой в проростках озимого рапса 二氢-基因调节机制和转录因子Hy5外源5-氨基酸在秋葵芽芽甘蓝预言中的转录因子

Doklady Akademii nauk Pub Date : 2020-07-09 DOI: 10.29235/1561-8323-2020-64-3-317-324

Н. Г. Аверина, А. В. Емельянова, Т. Г. Каляга, С. М. Савина

{"title":"Молекулярно-генетические механизмы регуляции дигидрофлавонол редуктазы и транскрипционного фактора Hy5 экзогенной 5-аминолевулиновой кислотой в проростках озимого рапса","authors":"Н. Г. Аверина, А. В. Емельянова, Т. Г. Каляга, С. М. Савина","doi":"10.29235/1561-8323-2020-64-3-317-324","DOIUrl":"https://doi.org/10.29235/1561-8323-2020-64-3-317-324","url":null,"abstract":"The effect of exogenous 5-aminolevulinic acid (ALA) on the activity of dihydroflavonol-4-reductase (DFR), the expression of the dfr gene and the hy5 gene of the transcription factor Hy5 and the light effect of different intensities in combination with the ALA action on the accumulation of anthocyanins in cotyledonous leaves of winter rape (Brassica napus L.) were studied. It was shown that the stimulation of the accumulation of anthocyanins under the exogenous ALA action at the molecular level was provided by increasing the expression level of the dfr and hy5 genes and the activity of the DFR enzyme. Increasing the light intensity from 40.5 to 66.2 μmol photons/m 2 ·s enhanced the ability of plants to accumulate anthocyanins on average by 35 %. The ALA action at concentrations of 50, 100, 150 and 200 mg/L led to an additional increase in the accumulation of anthocyanins at the two used levels of illumination, and in a dose-dependent manner. The stimulating effect of ALA under high light intensity was much higher than in the case of lower illumination. Thus, the stimulation of the anthocyanin accumulation under illumination of 40.5 μmol photons/m 2 ·s was 106 % when using 50 mg/L ALA, 165 % – when using 100 mg/L ALA, 222 % – in the case of 150 mg/L ALA and 350 % – under the action of 200 mg/L ALA compared with light control without of ALA treatment. At an illumination of 66.2 μmol photons/m 2 ·s, these indicators were 164, 262, 359 and 583 % respectively. Thus, it was demonstrated that the stimulation of the accumulation of anthocyanins under the action of ALA in winter rape plants was due to its positive effect on the transcription of the dfr and hy5 genes at the molecular level.","PeriodicalId":11227,"journal":{"name":"Doklady Akademii nauk","volume":"24 1","pages":"317-324"},"PeriodicalIF":0.0,"publicationDate":"2020-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84841593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Структурные и оптические свойства оксида кремния, имплантированного ионами цинка: влияние степени пересыщения и термообработки 离子锌植入的氧化物的结构和光学特性:过热程度和热处理的影响

Doklady Akademii nauk Pub Date : 2020-07-08 DOI: 10.29235/1561-8323-2020-64-3-273-281

М. А. Моховиков, О. В. Мильчанин, И. Н. Пархоменко, Фадей Фадеевич Комаров, Л. А. Власукова, Д. С. Королев, А. В. Мудрый, В. Д. Живулько, Арно Янсе ван Вуурен

{"title":"Структурные и оптические свойства оксида кремния, имплантированного ионами цинка: влияние степени пересыщения и термообработки","authors":"М. А. Моховиков, О. В. Мильчанин, И. Н. Пархоменко, Фадей Фадеевич Комаров, Л. А. Власукова, Д. С. Королев, А. В. Мудрый, В. Д. Живулько, Арно Янсе ван Вуурен","doi":"10.29235/1561-8323-2020-64-3-273-281","DOIUrl":"https://doi.org/10.29235/1561-8323-2020-64-3-273-281","url":null,"abstract":"The phase-structural composition of a silica film grown on Si substrate implanted with Zn ions at room temperature with different fluences has been studied using transmission electron microscopy and electron diffraction. The small clusters (1–2 nm) and the large clusters (5–7 nm) have been formed in as-implanted silica films with the Zn concentration of 6–7 at % and 16–18 at %, respectively. Furnace annealing at 750 °С for two hours results both in the formation of the orthorhombic Zn 2 SiO 4 phase (space group R-3) in the case of low fluence (5 · 10 16 cm –2 ) and in the formation of the cubic ZnO phase (space group F-43m) in the case of high fluence (1 · 10 17 cm –2 ). It has been shown that impurity loss during implantation and subsequent annealing increase with fluence of implanted ions. The fraction of Zn atoms in clusters has been estimated to be 15 % and 18 % for fluences (5 · 10 16 cm –2 ) and (1 · 10 17 cm –2 ), respectively. It has been shown that residual Zn impurities dissolved in silica matrix noticeably suppress the light-emitting properties of silica with embedded Zn 2 SiO 4 and ZnO nanocrystals.","PeriodicalId":11227,"journal":{"name":"Doklady Akademii nauk","volume":"517 1","pages":"273-281"},"PeriodicalIF":0.0,"publicationDate":"2020-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78149611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Коэффициентная устойчивость решений разностных схем, аппроксимирующих смешанные задачи для полулинейных гиперболических уравнений 半线性双曲方程近似混合问题的差分电路解系数稳定性

Doklady Akademii nauk Pub Date : 2020-05-17 DOI: 10.29235/1561-8323-2020-64-2-135-143

П. П. Матус, С. В. Лемешевский

引用次数: 0

Role of forests in maintaining a water balance in the territory of Belarus 森林在维持白俄罗斯境内水平衡方面的作用

Doklady Akademii nauk Pub Date : 2020-05-17 DOI: 10.29235/1561-8323-2020-64-2-225-232

S. Lysenko, V. Loginov

引用次数: 2

Total cross section of the reaction γd → π–pp in the threshold energy region 反应的总截面γd→π-pp在阈能区

Doklady Akademii nauk Pub Date : 2020-05-17 DOI: 10.29235/1561-8323-2020-64-2-159-163

E. Kokoulina, M. Levchuk, M. Nevmerzhitsky, R. Shulyakovsky

引用次数: 1

Fabrication and properties of calcium pectinate hydrogel nanoparticles with trans-cinnamic acid 反式肉桂酸果胶酸钙水凝胶纳米颗粒的制备及性能研究

Doklady Akademii nauk Pub Date : 2020-05-17 DOI: 10.29235/1561-8323-2020-64-2-164-172

A. Kraskouski, V. Kulikouskaya, O. Molchan, K. Hileuskaya, V. Yurin, V. Agabekov

引用次数: 0

Productivity of pigs and the genealogical structure of the breeding herds of Belarusian meat breed 猪的生产力和白俄罗斯肉猪种猪群的家谱结构

Doklady Akademii nauk Pub Date : 2020-05-17 DOI: 10.29235/1561-8323-2020-64-2-245-256

I. Sheyko, E. Yanovich, N. V. Pristupa, T. N. Timoshenko, R. Sheyko, I. V. Anihovskaya, K. A. Kаpshevich

{"title":"Productivity of pigs and the genealogical structure of the breeding herds of Belarusian meat breed","authors":"I. Sheyko, E. Yanovich, N. V. Pristupa, T. N. Timoshenko, R. Sheyko, I. V. Anihovskaya, K. A. Kаpshevich","doi":"10.29235/1561-8323-2020-64-2-245-256","DOIUrl":"https://doi.org/10.29235/1561-8323-2020-64-2-245-256","url":null,"abstract":"The genealogical breed structure is represented by 5 main factory lines: Zubr, Zenit, Zaslon, Zont, Zefir. The genealogical structure of the Belarusian meat breed is formed using the generally accepted selection methods, including the assessment by pedigree, strict selection, targeted selection, the assessment of replacement young by productivity on elevers, the assessment of boars and queens by the offspring quality using the control feeding method. The targeted breeding work with herds of Belarusian meat breed pigs during a long period of time according to the long-term target program allows us to obtain large boars and queens that are characterized by the meat-type body. When assessing boar-producers of the Belarusian meat breed aged 36 months and older in all farms the values of the body live weight and length were on the average 305.3 kg and 179 cm respectively. As a result of studying the reproductive qualities of animals, it is established that, on the average, in the queens selected into the leading group in all farms, the indicators of prolificacy, the weight of one young pig at birth, the number of young pigs aged 21 days and milking ability were 11.74 animals and 1.11 kg, 10.06 animals and 54.34 respectively. The highest growth rate from birth to a live weight of 100 kg was seen in the Zefir line animals – 550 g. The indicator of the growth in reaching a live weight of 100 kg was 181 days. The superiority over the mean indicators of animals of all lines according to these traits was 10.7 days, or 5.6 % and 31 g, or 6.0 % respectively. The Zalon and Zenit line offsprings were inferior to the boars of the rest lines in terms of the growth in reaching a live weight of 100 kg by 2.8–19 days. Their indicators of the daily average growth from birth to a live weight of 100 kg also appeared to be, on the average, lower by 6–49 g. The differences in the Zaslon line boars were confident ( р ≤ 0.05).","PeriodicalId":11227,"journal":{"name":"Doklady Akademii nauk","volume":"66 1","pages":"245-256"},"PeriodicalIF":0.0,"publicationDate":"2020-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81563397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis of brazzein using the bacterial cell-free protein synthesis system 利用细菌无细胞蛋白合成系统合成铜黄蛋白

Doklady Akademii nauk Pub Date : 2020-03-20 DOI: 10.29235/1561-8323-2020-64-1-71-77

I. Kazlouski, I. Belskaya, А. Zinchenko

引用次数: 0

Comparative analysis of the methods of producing lithium titanates 钛酸锂生产方法的比较分析

Doklady Akademii nauk Pub Date : 2020-03-19 DOI: 10.29235/1561-8323-2020-64-1-42-49

I. Matsukevich, A. Kulak, O. V. Polhovskaya, D. A. Kiiliomin

引用次数: 1

Estimating the effectiveness of protection against UV radiation with extracts of lichen Hypogymnia physodes in biological systems in vivo 在体内生物系统中估计地衣提取物对紫外线辐射的防护效果

Doklady Akademii nauk Pub Date : 2020-01-07 DOI: 10.29235/1561-8323-2019-63-6-747-754

S. Goncharov, A. E. Kozlov, M. V. Маtveyenkov, I. A. Cheshik

{"title":"Estimating the effectiveness of protection against UV radiation with extracts of lichen Hypogymnia physodes in biological systems in vivo","authors":"S. Goncharov, A. E. Kozlov, M. V. Маtveyenkov, I. A. Cheshik","doi":"10.29235/1561-8323-2019-63-6-747-754","DOIUrl":"https://doi.org/10.29235/1561-8323-2019-63-6-747-754","url":null,"abstract":"The reactivity of biological systems to UV-A/B and the photoprotective potential of skin applications of lichen extracts Hypogymnia physodes according to the biochemical parameters of blood and morphometric parameters of the skin were evaluated in vivo in laboratory mice. Ethanol, acetone and hexane–acetone extracts (1 % in dimethylsulfoxide) effectively absorb the UV range inducing the most erythema. On the 4th day after UV-A/Вirradiation, severe brown burns of the back skin, severe erythema and edema, scab formation were observed. In serum, there were significantly changed biochemical parameters – the prooxidant capacity, the level of nitrate/nitrite-ions NOx, advanced oxidation protein products AOPP, glutathioneperoxidase activity GPx increased, the level of SH-groups reduced. Solutions of extracts (5 %) in dimethylsulfoxide at a different degree exhibited the photoprotective effect: morphologically – in minimizing the burn symptoms of the skin (edema, erythema, scab); biochemically – in the regulation of these parameters at the control level. According to the set of biochemical and morphometric parameters, the most promising photoprotectors were acetone extracts. The prooxidant capacity, GPx, AOРР (in all cases reduced) and NOx showed a non-specificity and rather high sensitivity and are more suitable for estimating inflammatory and oxidative processes. The highest efficiency and the adequacy to the tasks and the specifications are shown by the level of protein SH-groups.","PeriodicalId":11227,"journal":{"name":"Doklady Akademii nauk","volume":"20 1","pages":"747-754"},"PeriodicalIF":0.0,"publicationDate":"2020-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86956868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0