Cytogenetic and Genome Research最新文献

{"title":"Retraction Statement","authors":"","doi":"10.1159/000523671","DOIUrl":"https://doi.org/10.1159/000523671","url":null,"abstract":"","PeriodicalId":11206,"journal":{"name":"Cytogenetic and Genome Research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47720033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front & Back Matter","authors":"Transf Ormati, C. Steinlein","doi":"10.1159/000522535","DOIUrl":"https://doi.org/10.1159/000522535","url":null,"abstract":"","PeriodicalId":11206,"journal":{"name":"Cytogenetic and Genome Research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47184592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contents Col. 161, 2021","authors":"C. Steinlein","doi":"10.1159/000522349","DOIUrl":"https://doi.org/10.1159/000522349","url":null,"abstract":"Human cytogenetics and genomics Maki Fukami Department of Molecular Endocrinology, National Research Institute for Child Health and Development 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan Tel. +81-3-5494-7025; Fax +81-3-5494-7026 fukami-m@ncchd.go.jp Joris Vermeesch Laboratory of Cytogenetics and Genome Research UZ Leuven, K.U. Leuven Campus Gasthuisberg Herestraat 49 B-3000 Leuven, Belgium Tel. (+32) 16 34 5941 joris.vermeesch@uzleuven.be","PeriodicalId":11206,"journal":{"name":"Cytogenetic and Genome Research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48690726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prenatal Genetic Diagnosis of Fetal Cystic Hygroma: A Retrospective Single-Center Study from China.","authors":"Yulin Zhou,&nbsp;Xingxiu Lu,&nbsp;Yanhong Zhang,&nbsp;Yunsheng Ge,&nbsp;Yasong Xu,&nbsp;Lili Wu,&nbsp;Yu Jiang","doi":"10.1159/000528600","DOIUrl":"https://doi.org/10.1159/000528600","url":null,"abstract":"<p><p>Fetal cystic hygroma (CH) is associated with poor prognosis and chromosomal anomalies. Recent studies have suggested that the genetic background of affected fetuses is essential for predicting pregnancy outcomes. However, the detection performance of different genetic approaches for the etiological diagnosis of fetal CH remains unclear. In this study, we aimed to compare the diagnostic efficiency of karyotyping and chromosomal microarray analysis (CMA) in a local fetal CH cohort, and tried to propose an optimized testing strategy that may help improve the cost-effectiveness of disease management. We reviewed all pregnancies that underwent invasive prenatal diagnosis between January 2017 and September 2021 at one of the largest prenatal diagnostic centers in Southeast China. We collected cases identified by the presence of fetal CH. Prenatal phenotypes and laboratory records of these patients were audited, collated, and analyzed. The detection rates of karyotyping and CMA were compared, and the concordance rate of these two methods was calculated. A total of 157 fetal CH cases were screened from 6,059 patients who underwent prenatal diagnosis. Diagnostic genetic variants were identified in 44.6% (70/157) of the cases. Karyotyping, CMA, and whole-exome sequencing (WES) identified pathogenic genetic variants in 63, 68, and 1 case, respectively. The Cohen's κ coefficient between karyotyping and CMA was 0.96, with a concordance of 98.0%. Of the 18 cases in which cryptic copy number variants <5 Mb were detected by CMA, 17 were interpreted as variants of uncertain significance, and the remaining cases were interpreted as pathogenic. Trio exome sequencing revealed a pathogenic homozygous splice site mutation in the PIGN gene in a case undiagnosed by CMA and karyotyping. Our study demonstrated that chromosomal aneuploidy abnormalities are the main genetic cause of fetal CH. Based on this, we recommend karyotyping combined with rapid aneuploidy detection as a first-tier approach for the genetic diagnosis of fetal CH. WES and CMA could improve the diagnostic yield when routine genetic tests fail to determine the cause of fetal CH.</p>","PeriodicalId":11206,"journal":{"name":"Cytogenetic and Genome Research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9550252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"B-Cell Acute Lymphoblastic Leukemia with iAMP21 in a Patient with Constitutional Ring Chromosome 21.","authors":"Vandana Baloda,&nbsp;Nidhi Aggarwal,&nbsp;Flavia G Rosado,&nbsp;Sarah Mackey,&nbsp;James Felker,&nbsp;Svetlana A Yatsenko","doi":"10.1159/000527025","DOIUrl":"https://doi.org/10.1159/000527025","url":null,"abstract":"<p><p>Pediatric B-cell acute lymphoblastic leukemia (B-ALL) is associated with various specific cytogenetic and molecular markers that significantly influence treatment and prognosis. Intrachromosomal amplification of chromosome 21 (iAMP21) defines a rare distinct cytogenetic subgroup of childhood B-ALL, which is characterized by amplification of region 21q22.12 comprising the RUNX1 gene. Constitutional structural chromosomal abnormalities involving chromosome 21 confer an increased risk for B-ALL with iAMP21. Here, we report the development of B-ALL with iAMP21 in a 9-year-old child with a constitutional ring chromosome 21, r(21)c, uncovered after B-ALL diagnosis. Cytogenetic and microarray analysis of the post-therapy sample revealed an abnormal chromosome 21 lacking a satellite and having a deletion of the terminal 22q22.3 region, consistent with a constitutional ring chromosome 21, r(21)(p11.2q22). On a retrospective analysis, this ring chromosome was observed in the normal cells in the pre-treatment diagnostic specimen. Constitutional ring chromosome 21 may remain undetected in patients with mild or no neurodevelopmental phenotype, posing an unknown lifelong risk of developing B-ALL with iAMP21. Individuals with constitutional structural chromosome 21 rearrangements such as ring 21 require a close surveillance and long-term follow-up studies to establish their risk of B-ALL relapse and possibility of developing other malignancies. Germline analysis is recommended to all pediatric patients with iAMP21-related B-ALL to rule out structural chromosome 21 rearrangements and to elucidate molecular mechanisms of iAMP21 formation.</p>","PeriodicalId":11206,"journal":{"name":"Cytogenetic and Genome Research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10773139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Girl-Boy Twins with Developmental Delay from 16p11.2 Triplication due to Biparental Inheritance from Two Parents with 16p11.2 Duplication.","authors":"Sidrah A Badar,&nbsp;Amy M Breman,&nbsp;Celanie K Christensen,&nbsp;Brett H Graham,&nbsp;Meredith R Golomb","doi":"10.1159/000521297","DOIUrl":"https://doi.org/10.1159/000521297","url":null,"abstract":"<p><p>The 16p11.2 duplication is a well-known cause of developmental delay and autism, but there are only 2 previously reported cases of 16p11.2 triplication. Both of the previously reported cases exhibited tandem triplication on a 16p11.2 duplication inherited from 1 parent. We report fraternal twins presenting with developmental delay and 16p11.2 triplication resulting from inheritance of a 16p11.2 duplicated homolog from each parent. This report also reviews the overlapping features in previously published cases of 16p11.2 triplication, and possible implications are discussed.</p>","PeriodicalId":11206,"journal":{"name":"Cytogenetic and Genome Research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39904042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytogenetic Analysis of the Fungus-Farming Ant Cyphomyrmex rimosus (Spinola, 1851) (Formicidae: Myrmicinae: Attini) Highlights Karyotypic Variation.","authors":"Gisele Amaro Teixeira,&nbsp;Gabriela de Figueiredo Jacintho,&nbsp;Hilton Jeferson Alves Cardoso de Aguiar,&nbsp;Denilce Meneses Lopes,&nbsp;Luísa Antônia Campos Barros","doi":"10.1159/000529607","DOIUrl":"https://doi.org/10.1159/000529607","url":null,"abstract":"<p><p>The fungus-farming ant genus Cyphomyrmex (subtribe Attina, clade Neoattina) comprises 23 described species that are widely distributed throughout the Neotropics. Species within Cyphomyrmex have taxonomic issues such as Cyphomyrmex rimosus (Spinola, 1851) which is likely a species complex. Cytogenetics is a useful tool for evolutionary studies and understanding species with dubious taxonomy. In this study, we characterized the karyotype of C. rimosus from Viçosa, Minas Gerais State, southeastern Brazil using classical and molecular cytogenetic techniques to enrich the chromosomal information about Cyphomyrmex. The karyotype of C. rimosus from the rainforest of southeastern Brazil (2n = 22, 18m + 4sm) notably contrasts with that previously described for this species in Panama (2n = 32). This intraspecific chromosomal variation suggests the existence of a species complex within this taxon according to the previous hypothesis derived from morphological analysis. We detected GC-rich heterochromatic regions in C. rimosus and, using repetitive DNA probes, showed that this heterochromatin shares repetitive sequences with other Neoattina species already studied, enhancing the importance of this specific genome region in the understanding of Attina evolution. Mapping of microsatellite (GA)15 on C. rimosus was restricted to the euchromatic regions of all chromosomes. The single intrachromosomal rDNA sites observed in C. rimosus follow the general genomic organization trend of ribosomal genes in Formicidae. Our study extends the data of chromosome mapping on Cyphomyrmex and reinforces the importance of cytogenetic studies in different localities to better understand taxonomic issues in widely distributed taxa such as C. rimosus.</p>","PeriodicalId":11206,"journal":{"name":"Cytogenetic and Genome Research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10088682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fourth Report on Chicken Genes and Chromosomes 2022.","authors":"Jacqueline Smith,&nbsp;James M Alfieri,&nbsp;Nick Anthony,&nbsp;Peter Arensburger,&nbsp;Giridhar N Athrey,&nbsp;Jennifer Balacco,&nbsp;Adam Balic,&nbsp;Philippe Bardou,&nbsp;Paul Barela,&nbsp;Yves Bigot,&nbsp;Heath Blackmon,&nbsp;Pavel M Borodin,&nbsp;Rachel Carroll,&nbsp;Meya C Casono,&nbsp;Mathieu Charles,&nbsp;Hans Cheng,&nbsp;Maddie Chiodi,&nbsp;Lacey Cigan,&nbsp;Lyndon M Coghill,&nbsp;Richard Crooijmans,&nbsp;Neelabja Das,&nbsp;Sean Davey,&nbsp;Asya Davidian,&nbsp;Fabien Degalez,&nbsp;Jack M Dekkers,&nbsp;Martijn Derks,&nbsp;Abigail B Diack,&nbsp;Appolinaire Djikeng,&nbsp;Yvonne Drechsler,&nbsp;Alexander Dyomin,&nbsp;Olivier Fedrigo,&nbsp;Steven R Fiddaman,&nbsp;Giulio Formenti,&nbsp;Laurent A F Frantz,&nbsp;Janet E Fulton,&nbsp;Elena Gaginskaya,&nbsp;Svetlana Galkina,&nbsp;Rodrigo A Gallardo,&nbsp;Johannes Geibel,&nbsp;Almas Gheyas,&nbsp;Cyrill John P Godinez,&nbsp;Ashton Goodell,&nbsp;Jennifer A M Graves,&nbsp;Daren K Griffin,&nbsp;Bettina Haase,&nbsp;Jian-Lin Han,&nbsp;Olivier Hanotte,&nbsp;Lindsay J Henderson,&nbsp;Zhuo-Cheng Hou,&nbsp;Kerstin Howe,&nbsp;Lan Huynh,&nbsp;Evans Ilatsia,&nbsp;Erich Jarvis,&nbsp;Sarah M Johnson,&nbsp;Jim Kaufman,&nbsp;Terra Kelly,&nbsp;Steve Kemp,&nbsp;Colin Kern,&nbsp;Jacob H Keroack,&nbsp;Christophe Klopp,&nbsp;Sandrine Lagarrigue,&nbsp;Susan J Lamont,&nbsp;Margaret Lange,&nbsp;Anika Lanke,&nbsp;Denis M Larkin,&nbsp;Greger Larson,&nbsp;John King N Layos,&nbsp;Ophélie Lebrasseur,&nbsp;Lyubov P Malinovskaya,&nbsp;Rebecca J Martin,&nbsp;Maria Luisa Martin Cerezo,&nbsp;Andrew S Mason,&nbsp;Fiona M McCarthy,&nbsp;Michael J McGrew,&nbsp;Jacquelyn Mountcastle,&nbsp;Christine Kamidi Muhonja,&nbsp;William Muir,&nbsp;Kévin Muret,&nbsp;Terence Murphy,&nbsp;Ismael Ng'ang'a,&nbsp;Masahide Nishibori,&nbsp;Rebecca E O'Connor,&nbsp;Moses Ogugo,&nbsp;Ron Okimoto,&nbsp;Ochieng Ouko,&nbsp;Hardip R Patel,&nbsp;Francesco Perini,&nbsp;María Ines Pigozzi,&nbsp;Krista C Potter,&nbsp;Peter D Price,&nbsp;Christian Reimer,&nbsp;Edward S Rice,&nbsp;Nicolas Rocos,&nbsp;Thea F Rogers,&nbsp;Perot Saelao,&nbsp;Jens Schauer,&nbsp;Robert Schnabel,&nbsp;Valerie Schneider,&nbsp;Henner Simianer,&nbsp;Adrian Smith,&nbsp;Mark P Stevens,&nbsp;Kyle Stiers,&nbsp;Christian Keambou Tiambo,&nbsp;Michele Tixier-Boichard,&nbsp;Anna A Torgasheva,&nbsp;Alan Tracey,&nbsp;Clive A Tregaskes,&nbsp;Lonneke Vervelde,&nbsp;Ying Wang,&nbsp;Wesley C Warren,&nbsp;Paul D Waters,&nbsp;David Webb,&nbsp;Steffen Weigend,&nbsp;Anna Wolc,&nbsp;Alison E Wright,&nbsp;Dominic Wright,&nbsp;Zhou Wu,&nbsp;Masahito Yamagata,&nbsp;Chentao Yang,&nbsp;Zhong-Tao Yin,&nbsp;Michelle C Young,&nbsp;Guojie Zhang,&nbsp;Bingru Zhao,&nbsp;Huaijun Zhou","doi":"10.1159/000529376","DOIUrl":"10.1159/000529376","url":null,"abstract":"none.","PeriodicalId":11206,"journal":{"name":"Cytogenetic and Genome Research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10217896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracking Chromosomal Origins in the Northern Italy System of Metacentric Races of the House Mouse.","authors":"Mabel D Giménez,&nbsp;Jonathan J Hughes,&nbsp;Moira Scascitelli,&nbsp;Sofia I Gabriel,&nbsp;Daniel W Förster,&nbsp;Thadsin Panithanarak,&nbsp;Heidi C Hauffe,&nbsp;Jeremy B Searle","doi":"10.1159/000527106","DOIUrl":"https://doi.org/10.1159/000527106","url":null,"abstract":"<p><p>The Western European house mouse is chromosomally diverse, with diploid karyotypes ranging from the standard 40 telocentric chromosomes down to 22 chromosomes. Karyotypes are modified through Robertsonian (Rb) fusion of 2 telocentrics into a single metacentric, occurring repeatedly with fixation, and whole-arm reciprocal translocations (WARTs) generating additional novel karyotypes. Over 100 metacentric populations (chromosomal races) have been identified, geographically clustered into \"systems.\" Chromosomal races within systems often hybridise, and new races may emerge through this hybridisation (\"zonal raciation\"). We wished to determine the degree to which chromosomal races in a system have evolved independently or share common ancestry. Recombination between chromosomes from hybridising chromosomal races can erase the signals associated with a particular metacentric of interest, making inferences challenging. However, reduced recombination near the centromeres of chromosomal race-specific metacentrics makes centromere-adjacent markers ideal for solving this problem. For the Northern Italy System (NIS), we used microsatellite markers near the centromere to test previous hypotheses about evolutionary relationships of 5 chromosomal races. We chose markers from chromosomes 1, 3, 4, and 6, all of which comprise one arm of a metacentric in at least 2 of these NIS metacentric populations. We used estimates of FST and RST, as well as principal components analyses and neighbour-joining phylogenetic analyses, to infer evolutionary relationships between these 5 chromosomal races and neighbouring mice with the standard karyotype. We showed that the metacentric populations form a single grouping distinct from the standard populations, consistent with their common origin and consistent with a parsimonious sequence of chromosomal rearrangements to explain the relationship of the chromosomal races. That origin and evolution of the chromosomal races in the system would have involved Rb fusions, explaining the occurrence of chromosomal races with diploid numbers as low as 22. However, WARTs and zonal raciation have also been inferred, and the rare occurrence of chromosome 1 in different metacentrics in closely related chromosomal races is almost certainly explained by a WART. Our results with centromeric microsatellites are consistent with the above scenarios, illustrating, once again, the value of markers in the centromeric region to test evolutionary hypotheses in house mouse chromosomal systems.</p>","PeriodicalId":11206,"journal":{"name":"Cytogenetic and Genome Research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10413737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Neo-X Does Not Form a Barr Body but Shows a Slightly Condensed Structure in the Okinawa Spiny Rat (Tokudaia muenninki).","authors":"Ryoma Kudo, Ikuya Yoshida, Luisa Matiz Ceron, Shusei Mizushima, Yoko Kuroki, Takamichi Jogahara, Asato Kuroiwa","doi":"10.1159/000531275","DOIUrl":"10.1159/000531275","url":null,"abstract":"<p><p>X chromosome inactivation (XCI) is an essential mechanism for gene dosage compensation between male and female cells in mammals. The Okinawa spiny rat (Tokudaia muenninki) is a native rodent in Japan with XX/XY sex chromosomes, like most mammals; however, the X chromosome has acquired a neo-X region (Xp) by fusion with an autosome. We previously reported that dosage compensation has not yet evolved in the neo-X region; however, X-inactive-specific transcript (Xist) RNA (long non-coding RNA required for the initiation of XCI) is partially localized in the region. Here, we show that the neo-X region represents an early chromosomal state in the acquisition of XCI by analyses of heterochromatin and Barr body formation. We found no evidence for heterochromatin formation in the neo-X region by R-banding by acridine orange (RBA) assays and immunostaining of H3K27me3. Double-immunostaining of H3K27me3 and HP1, a component of the Barr body, revealed that the entire ancestral X chromosome region (Xq) showed a bipartite folded structure. By contrast, HP1 was not localized to the neo-X region. However, BAC-FISH revealed that the signals of genes on the neo-X region of the inactive X chromosome were concentrated in a narrow region. These findings indicated that although the neo-X region of the inactive X chromosome does not form a complete Barr body structure (e.g., it lacks HP1), it forms a slightly condensed structure. These findings combined with the previously reported partial binding of Xist RNA suggest that the neo-X region exhibits incomplete inactivation. This may represent an early chromosomal state in the acquisition of the XCI mechanism.</p>","PeriodicalId":11206,"journal":{"name":"Cytogenetic and Genome Research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9565367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}