Dental and Medical Problems最新文献

{"title":"Comparison of the efficacy of simple and combined oral rinses with chlorhexidine digluconate against selected bacterial and yeast species: An in vitro study.","authors":"Rafał Wiench, Anna Kuśka-Kiełbratowska, Małgorzata Kępa, Zuzanna Grzech-Leśniak, Maciej Jabłoński, Jan Kiryk, Kinga Grzech-Leśniak, Dariusz Skaba","doi":"10.17219/dmp/187834","DOIUrl":"https://doi.org/10.17219/dmp/187834","url":null,"abstract":"<p><strong>Background: </strong>Chlorhexidine digluconate (CHG) is considered the most effective and safe antimicrobial agent in dentistry. Recently, it has often been produced in the form of preparations with additional substances that may modify its effect.</p><p><strong>Objectives: </strong>The aim of the present study was to compare the efficacy of various simple and combined CHG rinses against selected bacterial and yeast strains.</p><p><strong>Material and methods: </strong>This research followed the European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines, using the disk diffusion method. The study was carried out on the following reference strains: Staphylococcus aureus ATCC 43300; Streptococcus pyogenes ATCC 19615; Pseudomonas aeruginosa ATCC 27853; Enterococcus faecalis ATCC 29212; Candida albicans ATCC 10231; C. glabrata ATCC 15126; C. krusei ATCC 14243; and C. parapsilosis ATCC 22019. The disinfection efficacy of 9 commercial mouthwashes with CHG was assessed (4 simple preparations, with different concentrations (0.5%, 0.2%, 0.12%, and 0.05%), and 5 combined preparations (0.2% CHG with adjuvants)) by comparing the size of the growth inhibition zones (GIZs) of microorganisms after 24 h of incubation.</p><p><strong>Results: </strong>Growth inhibition zones were observed around all tested substances, for all assessed strains. In simple preparations, the greatest reduction in growth was observed for Gram-positive bacteria. Statistically significantly smaller GIZs were recorded for P. aeruginosa and all Candida strains. The size of GIZ also depended on the CHG concentration used. In combined preparations, the greatest reduction in growth was also observed for Gram-positive bacteria (especially large GIZs for S. aureus when using 0.2% CHG with colostrum). Statistically significantly smaller GIZs were observed for P. aeruginosa and all yeasts. None of the evaluated adjuvants impaired the disinfecting effect of CHG.</p><p><strong>Conclusions: </strong>The evaluated combined preparations of CHG showed disinfecting efficacy against selected bacterial and fungal strains comparable to that of simple formulations. The combination of 0.2% CHG with colostrum showed the additive synergism of antimicrobial activity against the S. aureus ATCC 43300 strain.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analyzing and exploring Graph Attention Networks and protein-based language models for predicting Porhyromonas gingivalis resistant efflux protein sequences.","authors":"Pradeep Kumar Yadalam, Prabhu Manickam Natarajan, Naresh Shetty, Maria Maddalena Marrapodi, Hande Uzunçıbuk, Diana Russo, Marco Cicciù, Giuseppe Minervini","doi":"10.17219/dmp/186143","DOIUrl":"https://doi.org/10.17219/dmp/186143","url":null,"abstract":"<p><strong>Background: </strong>Antimicrobial resistance (AMR) must be predicted to combat antibiotic-resistant illnesses. Based on high-priority AMR genomes, it is possible to track resistance and focus treatment to stop global outbreaks. Large language models (LLMs) are essential for identifying Porhyromonas gingivalis multiresistant efflux genes to prevent resistance. Antibiotic resistance is a serious problem; however, by studying specific bacterial genomes, we can predict how resistance develops and find better kinds of treatment.</p><p><strong>Objectives: </strong>This paper explores using advanced models to predict the sequences of proteins that make P. gingivalis resistant to treatment. Understanding this approach could help prevent AMR more effectively.</p><p><strong>Material and methods: </strong>This research utilized multi-drug-resistant efflux protein sequences from P. gingivalis, identified through UniProt ID A0A0K2J2N6_PORGN, and formatted as FASTA sequences for analysis. These sequences underwent rigorous detection and quality assurance processes to ensure their suitability for computational analysis. The study employed the DeepBIO framework, which integrates LLMs with deep attention networks to process FASTA sequences.</p><p><strong>Results: </strong>The analysis revealed that the Long Short-Term Memory (LSTM)-attention, ProtBERT and BERTGAT models achieved sensitivity scores of 0.9 across the board, with accuracy rates of 89.5%, 88.5% and 90.5%, respectively. These results highlight the effectiveness of the models in identifying P. gingivalis strains resistant to multiple drugs. Furthermore, the study assessed the specificity of the LSTM-attention, ProtBERT and BERTGAT models, which achieved scores of 0.89, 0.87 and 0.90, respectively. Specificity, or the genuine negative rate, measures the ability of a model to accurately identify non-resistant cases, which is crucial for minimizing false positives in AMR detection.</p><p><strong>Conclusions: </strong>When utilized clinically, this LLM approach will help prevent AMR, which is a global problem. Understanding this approach may enable researchers to develop more effective treatment strategies that target specific resistant genes, reducing the likelihood of resistance development. Ultimately, this approach could play a pivotal role in preventing AMR on a global scale.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":"62 2","pages":"265-273"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of tablets containing a paraprobiotic strain and the cranberry extract on caries incidence in preschool children: A randomized controlled trial.","authors":"Dorota Olczak-Kowalczyk, Anna Turska-Szybka, Svante Twetman, Dariusz Gozdowski, Paula Piekoszewska-Ziętek, Joanna Góra, Marta Wróblewska","doi":"10.17219/dmp/171895","DOIUrl":"10.17219/dmp/171895","url":null,"abstract":"<p><strong>Background: </strong>Preand probiotics may help restore a dysbiotic oral ecosystem. The first years of life provide a window of opportunity to modulate the composition of the oral microbiota and prevent disease.</p><p><strong>Objectives: </strong>The aim of the present study was to investigate the effect of a tablet containing inactivated Ligilactobacillus salivarius CECT 5317 and the cranberry extract on the development of caries in cariesactive preschool children.</p><p><strong>Material and methods: </strong>The study employed a randomized, placebo-controlled, double-blind design. Preschool children (N = 73) with at least one active carious lesion were enrolled and randomly assigned to the test group or the placebo group. The intervention period was 3 months. Caries was assessed according to the International Caries Detection and Assessment System (ICDAS) II criteria at baseline and after 9 months, and oral hygiene was evaluated with the simplified oral hygiene index (OHI-S). The salivary counts of Streptococcus mutans and Lactobacillus spp. were determined at baseline, and then after 3 and 9 months through the conventional cultivation on TYCSB and MRS agar, respectively.</p><p><strong>Results: </strong>Sixty children completed the trial (a dropout rate of 19%). The baseline caries prevalence was high in both groups (~71%) and there were no major differences between the groups with regard to background variables. The 9-month incidence of initial carious lesions (ICDAS 1+2) was significantly lower in the test group as compared to the placebo group (p < 0.05). The plaque levels, and the salivary counts of S. mutans and Lactobacillus spp. remained unchanged in both groups throughout the study.</p><p><strong>Conclusions: </strong>A daily intake of a tablet containing a paraprobiotic and the cranberry extract reduced the 9-month incidence of initial non-cavitated carious lesions in caries-active preschool children. The present study is one of the first to show the impact of synbiotics on the development of caries in children.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":"209-215"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of chlorhexidine gluconate and povidone-iodine mouthwash on cycle threshold values in patients infected with SARS-CoV-2.","authors":"Lilies Dwi Sulistyani, Teuku Zulfahmi Rizki, Budi Haryanto, Vera Julia, Arfan Badeges, Dwi Ariawan, Mohammad Adhitya Latief, Yudy Ardilla Utomo","doi":"10.17219/dmp/192493","DOIUrl":"https://doi.org/10.17219/dmp/192493","url":null,"abstract":"<p><strong>Background: </strong>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants exhibit different phenotypes and clinical manifestations in comparison to non-mutated viruses. Spike gene target failure (SGTF) is a characteristic feature of the gene in a novel variant that is recognized as highly transmissible. Several studies have demonstrated the virucidal effects of mouthwashes on SARS-CoV-2. Moreover, mouthwashes have proven beneficial for patients undergoing oral and maxillofacial surgery.</p><p><strong>Objectives: </strong>The present study aimed to analyze the effects of 2 different types of mouthwash (0.2% chlorhexidine gluconate and 1% povidone-iodine) on the cycle threshold (CT) values in coronavirus disease 2019 (COVID-19) patients with and without SGTF.</p><p><strong>Material and methods: </strong>This single-blind, non-randomized controlled clinical trial comprised 45 patients who were divided into 3 groups based on the intervention method: 0.2% chlorhexidine gluconate mouthwash; 1% povidone-iodine mouthwash; and mineral water (control group). The patients were instructed to gargle with the assigned solution 3 times a day for 5 days. Reverse transcription polymerase chain reaction (RT-PCR) tests were conducted at the time of initial diagnosis and on days 3 and 5. A normality test (Shapiro-Wilk test) was performed. Consequently, the non-parametric Friedman test was used.</p><p><strong>Results: </strong>The analysis revealed that the subjects who utilized mouthwashes exhibited higher CT values in comparison to the control group. Furthermore, 73% of patients who used 0.2% chlorhexidine gluconate presented with increased CT values, as indicated by a negative RT-PCR test on the 3rd day.</p><p><strong>Conclusions: </strong>Gargling with 0.2% chlorhexidine gluconate or 1% povidone-iodine for 30 s for at least 3 days has been demonstrated to increase CT values in both SGTF and non-SGTF COVID-19 patients. Hence, using the mouthwash may be considered for preoperative use in patients undergoing oral and maxillofacial surgery.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":"62 2","pages":"217-223"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143983600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of mechanical tongue cleaning on taste perception: A systematic review.","authors":"Fulvia Costantinides, Marta Gionechetti, Monica Baiana, Erica Vettori, Vanessa Nicolin, Roberto Di Lenarda","doi":"10.17219/dmp/172888","DOIUrl":"10.17219/dmp/172888","url":null,"abstract":"<p><p>The aim of the present literary review was to compile the most recent evidence regarding the impact of mechanical tongue cleaning on gustatory perception in patients with a coated tongue. The present study adhered to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. The bibliographic survey of PubMed®, Scopus, Web of Science, and Cochrane Library databases was conducted using the following combination of keywords and Boolean operators: (tongue brushing AND taste) OR (tongue cleaning AND taste) OR (tongue coating AND taste). The quality of studies and the risk of bias were assessed based on the checklist provided by Downs and Black.Four articles were selected for the review based on the established inclusion criteria. The analysis of the data showed a decrease in lingual coating post-brushing in all studies. All articles demonstrated an improvement in gustatory sensitivity following mechanical removal of the lingual patina, though not every study observed a statistically significant increase for the same flavor. The outcomes of this review suggest that mechanical cleaning of the dorsum of the tongue can increase gustatory perception, therefore, it could be considered a promising and cost-effective addition to daily oral hygiene practices.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":"361-369"},"PeriodicalIF":3.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary cytokines as a biomarker for diagnosis, prognosis and treatment of oral squamous cell carcinoma: A systematic review.","authors":"Gayathri Rengasamy, Hema Shree Kasirajan, Vishnu Priya Veeraraghavan, Pratibha Ramani, Gabriele Cervino, Giuseppe Minervini","doi":"10.17219/dmp/186664","DOIUrl":"10.17219/dmp/186664","url":null,"abstract":"<p><p>Oropharyngeal squamous cell carcinoma and oral squamous cell carcinoma (OSCC) are the most common types of head and neck squamous cell carcinoma. Oral squamous cell carcinoma is the 6th most prevalent epithelial malignancy worldwide, and it is known for having significant morbidity and mortality rates. The present systematic review aims to identify which cytokines can be used as salivary biomarkers for the prognosis, diagnosis and treatment of OSCC. The early detection of the tumor and its precursor lesions is critical for improving the survival rate, reducing costs and enhancing the quality of life following treatment for OSCC. The conducted literature search yielded 65 articles; however, only 8 articles met the inclusion and exclusion criteria and were selected for a systematic review. Eighty percent of the articles were review articles, encompassing case-control studies and longitudinal studies. In 50% of the studies, diagnostic meta-analyses were conducted. According to the reviewed articles, interleukin-1β (IL-1β), IL-6, IL-8, IL-1 receptor antagonist (IL-1RA), interferon-γ (IFN-γ), tumor necrosis factor (TNF) (-α, -β, -γ), and matrix metalloproteinase-9 (MMP-9) are potential markers for OSCC, with a sensitivity and specificity of 100%.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":"351-359"},"PeriodicalIF":3.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of tooth bleaching in patients with fixed orthodontic appliances: A systematic review.","authors":"Gustavo Teodoro Costa Lizarelli, Rafaela Manente, Aline Souza-Gabriel, Silmara Aparecida Milori Corona","doi":"10.17219/dmp/171606","DOIUrl":"10.17219/dmp/171606","url":null,"abstract":"<p><p>Patients seeking a harmonious smile that combines satisfactory aesthetics and stable occlusion raise questions about the concomitant association of treatments. This systematic review aimed to answer the following question: \"Does the application of hydrogen peroxide on teeth with fixed metallic orthodontic brackets interfere with the dental bleaching effect?\". The PICOS (Population, Intervention, Comparison, Outcome, and Study design) strategy and the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines were adhered to during the review process. A comprehensive search strategy was implemented, encompassing the PubMed®, Web of Science, Scopus, Embase, and LILACS databases. The risk of bias was assessed using the Cochrane risk of bias (RoB) 2.0 tool. The initial search yielded 51 articles, which were then filtered to remove duplicates. The titles and abstracts of 26 studies were subsequently reviewed. Eleven articles were selected for full-text reading, and after applying the eligibility criteria, 3 studies were included for qualitative analysis. Two studies were classified as having a low risk of bias, while 1 study was classified as having some concerns. The bleaching was satisfactory with 8% and 10% hydrogen peroxide (HP) applied for 45 min daily over 10 days, even with fixed orthodontic brackets. However, a single study that employed the in-office technique (38% HP) demonstrated that fixed orthodontic appliances influenced external tooth bleaching. Tooth bleaching using the home bleaching technique yielded effective results when performed alongside orthodontic treatment.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":"343-350"},"PeriodicalIF":3.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of processing techniques of monolithic lithium disilicate ceramic on wear resistance against zirconia antagonist.","authors":"Anselmo Agostinho Simionato, Alessandra De Sousa Ramos, Olívia Breda Moss, Adriana Cláudia Lapria Faria, Renata Cristina Silveira Rodrigues, Ricardo Faria Ribeiro","doi":"10.17219/dmp/171899","DOIUrl":"10.17219/dmp/171899","url":null,"abstract":"<p><strong>Background: </strong>The durability of the materials used in the prosthesis is a determining factor for the success of the rehabilitation.</p><p><strong>Objectives: </strong>The aim of the study was to evaluate the wear resistance of monolithic lithium disilicate ceramics processed by heat pressing and computer-aided design/computer-aided manufacturing (CAD/CAM) methods, with a leucite-reinforced feldspathic ceramic processed by CAD/CAM serving as a control group.</p><p><strong>Material and methods: </strong>Monolithic lithium disilicate ceramic samples, processed as CAD/CAM milled blocks or heat-pressed ingots, were tested against flat zirconia antagonists. A CAD/CAM leucite-reinforced feldspathic ceramic was used as the control specimen. Conical specimens were made for each group and subjected to thermomechanical cycling with a flat zirconia antagonist. The roughness of the conical and flat specimens was evaluated before and after thermomechanical cycling. The height loss in the conical specimens was also assessed. The data concerning height loss was analyzed using one-way analysis of variance (ANOVA) and Tukey's post hoc test. The data obtained from the roughness analysis of the conical and flat samples was evaluated using a linear model of repeated measures and Bonferroni complementary test.</p><p><strong>Results: </strong>A significant difference was observed in the extent of height loss among the ceramics under consideration. Amber® Mill (AMM) exhibited a lesser extent of height loss in comparison to Rosetta® SP (RSP) (p = 0.010) and Rosetta® BM (RBM) (p = 0.005), yet it demonstrated congruence with Rosetta® SM (RSM) (p = 0.525). Additionally, a significant difference was noted between the initial and final roughness values for both the conical (p = 0.017) and flat (p < 0.05) samples, with the final roughness values being lower than the initial values.</p><p><strong>Conclusions: </strong>The attrition between ceramic surfaces and zirconia led to a decrease in superficial roughness (Sa). In the context of lithium disilicate ceramics, milled ceramics demonstrated superior performance in terms of wear behavior. The tested feldspathic ceramic exhibited a lower wear resistance compared to the milled lithium disilicate ceramics; however, its wear behavior was similar to that of the heat-pressed lithium disilicate ceramic.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":"62 2","pages":"285-292"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of high-consumption beverages on the color, and surface roughness and microhardness of resin-matrix ceramics.","authors":"Ebele Adaobi Silva, Anselmo Agostinho Simionato, Olívia Breda Moss, Adriana Cláudia Lapria Faria, Renata Cristina Silveira Rodrigues, Ricardo Faria Ribeiro","doi":"10.17219/dmp/171320","DOIUrl":"https://doi.org/10.17219/dmp/171320","url":null,"abstract":"<p><strong>Background: </strong>Resin-matrix ceramics have been developed to combine the high esthetics of ceramics with the mechanical properties of composite resin. The surface changes of these materials when exposed to highly consumed beverages are still not fully elucidated and need further research.</p><p><strong>Objectives: </strong>The aim of the present study was to evaluate the color stability, and surface roughness and microhardness of a polymer-infiltrated ceramic network (PICN) (VITA ENAMIC® - VE) and a resin nanoceramic (Ambarino® High-Class - AH), used to obtain computer-aided design/computer-aided manufacturing (CAD/CAM) restorations, after immersion in high-consumption beverages.</p><p><strong>Material and methods: </strong>A total of 96 specimens were prepared (n = 48 per group - VE and AH), further subdivided into 6 groups (n = 8) according to the beverage in which they were immersed. The color change (ΔE00), surface roughness (Ra) and microhardness (KHN) measurements were made before and after immersion in the beverages.</p><p><strong>Results: </strong>The materials presented significant differences in ΔE00 when immersed in distilled water (p = 0.043), coffee (p = 0.010) and red wine (p < 0.001). Acceptable values for ΔE00 were recorded for distilled water and the energy drink with regard to AH, and for the energy drink and orange juice with regard to VE. Regarding the Ra data, the VE samples showed a difference after immersion in all the tested beverages, while AH differed only when immersed in distilled water and the energy drink. Immersion in the beverages seemed to reduce KHN in the tested materials, although significant differences were detected only in the VE samples from the energy drink (p < 0.001) and orange juice (p < 0.001) groups.</p><p><strong>Conclusions: </strong>Based on the results, it can be concluded that the beverages tested may affect the esthetics and surface characteristics of the materials tested.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":"62 2","pages":"299-307"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circulating biomarkers of nitrosative stress, protein glycoxidation and inflammation in maxillofacial surgery patients treated with titanium implants.","authors":"Bożena Antonowicz, Jan Borys, Anna Zalewska, Małgorzata Żendzian-Piotrowska, Kamila Łukaszuk, Łukasz Woźniak, Mariusz Szuta, Mateusz Maciejczyk","doi":"10.17219/dmp/188863","DOIUrl":"10.17219/dmp/188863","url":null,"abstract":"<p><strong>Background: </strong>Titanium (Ti) biomaterials are widely used in the surgical management of maxillofacial trauma, in oncology and orthognathic surgery. Although Ti is considered highly biocompatible, adverse reactions at the implant site have been reported in numerous clinical studies. However, the influence of Ti mandibular implants on glutathione metabolism, nitrosative stress and systemic inflammation has not been investigated to date.</p><p><strong>Objectives: </strong>The study aimed to evaluate the acute (short-term) effects of Ti mandibular implants on the circulating biomarkers of the antioxidant defense system, on oxidative and nitrosative stress, as well as the inflammatory response of the blood plasma/erythrocytes, in maxillofacial surgery patients compared to the control group.</p><p><strong>Material and methods: </strong>The experimental group consisted of 40 patients with bilateral mandibular fractures, who received osteosynthesis treatment with the use of Ti-6Al-4V alloy miniplates and screws. The control group comprised 40 ageand gender-matched patients who were qualified for the surgical treatment of craniofacial defects through bimaxillary osteotomy.</p><p><strong>Results: </strong>An increase in the activity of pro-oxidant enzymes (↑ nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX), ↑ xanthine oxidase (XO)), impaired glutathione metabolism (↓ total glutathione, ↑ oxidized glutathione (GSSG), ↓ reduced glutathione (GSH), ↓ redox status), higher levels of oxidative stress (↓ total thiols, ↑ malondialdehyde (MDA), ↑ lipid hydroperoxides (LOOHs)), ↓ total antioxidant status (TAS)), carbonyl stress (↑ dityrosine, ↑ N-formylkynurenine) and nitrosative stress (↑ nitric oxide (NO), ↑ S-nitrosothiols, ↑ peroxynitrite, ↑ nitrotyrosine), as well as an intensified systemic inflammatory response (↑ interleukin (IL)-1β, ↑ IL-6), were observed in maxillofacial surgery patients.</p><p><strong>Conclusions: </strong>Despite the fact that the study examined only the circulating biomarkers of redox balance and inflammation, the results suggest that a systemic inflammatory response can be triggered by local immune reactions. Systemic inflammation and oxidative stress may stem from an early adaptive immune response to foreign objects in the body. Although further research is required, the removal of the existing Ti mandibular implants should be considered.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":"225-236"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}