Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA最新文献_第8页

[Evaluation of quality of life of hypertensive patients]. [高血压患者生活质量评价]。

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

Ying Chen, Sheng-yong Wang, Gui-bo Chi, Xia-hua Yu, Cheng Hong, An-rong Li

{"title":"[Evaluation of quality of life of hypertensive patients].","authors":"Ying Chen, Sheng-yong Wang, Gui-bo Chi, Xia-hua Yu, Cheng Hong, An-rong Li","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To evaluate the quality of life (QOL) of patients with hypertension.Methods: Totally 319 patients with hypertension were investigated for their QOL in comparison with 319 healthy controls using the World Health Organization Quality of Life assessment instrument (WHOQOL-100).Results: The scores of physical functions, psychological condition, independent ability, social relation, living environment, and personal faith and the total score of QOL-100 were significantly lower in the hypertensive patients than in the normal subjects (P<0.01). After adjusting the confounding factors of other diseases, the scores of all the items with the exception of personal faith and the total score of QOL-100 were all lower in the patients than in the controls (P<0.01). Hostelling T2 test and multivariate analysis of variance showed significant differences in the QOL between the hypertensive patients and the controls in the 6 domains synthesized (P<0.01), further demonstrating lowered QOL of hypertensive patients in comparison with the healthy population.Conclusion: Improvement of the hypertensive patients' QOL in addition to effective blood pressure control is a basic target in clinical therapy of hypertension.","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25705735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of multidrug resistance with up-regulation of protein kinase C expression in KBV200 cells. KBV200细胞多药耐药与蛋白激酶C表达上调的相关性

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

Ya-wei Yuan, Ai-min Sun, Chuan-gang Li

{"title":"Correlation of multidrug resistance with up-regulation of protein kinase C expression in KBV200 cells.","authors":"Ya-wei Yuan, Ai-min Sun, Chuan-gang Li","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate protein kinase C (PKC) expression and its association with multidrug resistance (MDR) in KBV200 cells.Methods: KBV200 cells were preincubated with PKC activator phorbol-12-myristate-13-acetate (PMA, 200 nmol/L) and PKC activity was assayed by measurement of peptide substrate (32)P incorporation from [gamma-(32)P]ATP, with the cells without PMA preincubation serving as the control. Western blotting was performed for assessing the expression of PKC isoform, and the cell inhibition rate was evaluated by MTT assay.Results: PMA preincubation of the cells significantly enhanced the activity of the total PKC and the membrane fraction, but lowered the PKC activity of the cytosol fraction, as compared with the cells without PMA treatment (P<0.01). PKC-alpha expression was upregulated in the membrane fraction and down-regulated in the cytosol fraction in KBV200 cells after PMA preincubation. PKCbeta expression was slightly elevated in the cytosol fraction but exhibited no obvious changes in the membrane fraction after PMA pretreatment of the cells. The values of IC(50) of vincristine and adriamycin in PMA-treated cells were increased to 2275.5 nmol/L and 233.25 nmol/L, respectively (P<0.01).Conclusion: PMA can increase the multidrug resistance of KBV200 cells, which suggests the possible involvement of PKC in the mechanism of multidrug resistance of tumor cells.","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25706376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Percutaneous port-catheter system implantation via left subclavian artery with drug perfusion for diabetic foot: report of one case]. 【经左锁骨下动脉经皮静脉导管系统植入术加药物灌注治疗糖尿病足1例】。

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

Mei-ping Guan, Jie Shen, Chen-zhong Li

引用次数: 0

[Expression of L-type calcium channel alpha1C subunit in adult rat heart]. 成年大鼠心脏中l型钙通道α - 1c亚基的表达

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

Yan Ou, Xiao-lin Niu, Fu-xian Ren, Ying Zhang, Feng-dong Ling

{"title":"[Expression of L-type calcium channel alpha1C subunit in adult rat heart].","authors":"Yan Ou, Xiao-lin Niu, Fu-xian Ren, Ying Zhang, Feng-dong Ling","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the expression and distribution of L-type calcium channel alpha1C subunits in adult rat heart.Methods: HE staining was applied on the frozen sections of adult rat heart to identify the sinoatrial node (SAN), atrioventricular node (AVN), and posterior nodal extension (PNE). The protein expression of L-type calcium channel alpha1C in adult rat heart and its cellular localization were examined by Western blotting and immunohistochemistry, respectively.Results: L-type calcium channel alpha1C subunit was immunolocalized on the membrane of the myocardial cells, and its expression increased gradually in the SAN, AVN, PNE, right atrium and right ventricle. The protein level of L-type calcium channel alpha1C in the AVN was similar to that in the PNE (P>0.05), and its level in the right atrium and ventricle were significantly higher than those in the SAN and AVN (P<0.01). The results of Western blotting were well consistent with the results of immunohistochemistry.Conclusion: The expression of L-type calcium channel alpha1C subunit may play a role in the electrophysiological functions of the heart.","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25725876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Misdiagnosis of dementia with Lewy bodies: report of one case]. 【路易体痴呆误诊1例报告】。

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

Yu-ming Li, Lu-ni Wang

引用次数: 0

[Sequence analysis of the mutated genes in CTXEVC Phi and nct-CTXnew Phi genomes in Vibrio cholerae JS94484 strain]. [霍乱弧菌JS94484株CTXEVC Phi和nct-CTXnew Phi基因组突变基因序列分析]。

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

Yong-yu Rui, Biao Kan, Shou-yi Gao, Yan-qing Liu, Guo-ming Qi

{"title":"[Sequence analysis of the mutated genes in CTXEVC Phi and nct-CTXnew Phi genomes in Vibrio cholerae JS94484 strain].","authors":"Yong-yu Rui, Biao Kan, Shou-yi Gao, Yan-qing Liu, Guo-ming Qi","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To analyze the sequences of the mutated genes in CTX(EVC)Phi and nct-CTX(new)Phi genomes in Vibrio cholerae JS94484 strain.Methods: The mutated genes in CTX(EVC)Phi and nct-CTX(new)Phi genome were obtained by PCR, sequenced and analyzed.Results: ig1, rstR, ig2, and ctxAB genes in CTX(EVC)Phi genome of V. cholerae strain JS94484 were highly homologous with those of standard EVC strain N16961, while ig1, rstR and ig2 genes in nct-CTX(new)Phi genome of the strain JS94484 shared low homology with those of the other 3 biotypes of V. cholerae. Considerable difference was detected in the last 60 bp of zot genes between CTX(EVC)Phi and nct-CTX(new)Phi genomes, indicating possible difference in the amino sequences of the Zot proteins encoded by these two genes. The sequence of toxin-coregulated pilus A subunit gene (tcpA) of the strain JS94484 was identical with that of strain N16961.Conclusion: ig1, rstR and ig2 genes of nct-CTX(new)Phigenome are of a novel type, and their functions await further investigation.","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25705729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Effects of benazepril combined with valsartan on congestive heart failure]. [苯那普利联合缬沙坦治疗充血性心力衰竭的疗效]。

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

Jian-feng Ye, Dong-sheng Liu

引用次数: 0

[Effect of interferon alpha on liver fibrosis in patients with chronic hepatitis B]. [干扰素对慢性乙型肝炎肝纤维化的影响]。

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

Hong Yu, Zhi-chen Cao, Jian-ying Geng, Zhan-guo He, Zheng-min Wang, Xiao-yun Sun, Zhong-hua Wang

{"title":"[Effect of interferon alpha on liver fibrosis in patients with chronic hepatitis B].","authors":"Hong Yu, Zhi-chen Cao, Jian-ying Geng, Zhan-guo He, Zheng-min Wang, Xiao-yun Sun, Zhong-hua Wang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To observe the histological changes in the fibrotic and inflammatory tissues in response to interferon alpha treatment in patients with chronic viral hepatitis B.Methods: Sixteen patients with chronic viral hepatitis B in S3-S4 stages established by pathological examination were treated with interferon alpha for 6-9 months, and the degree of liver fibrosis and inflammation were examined 3 times during the treatment. The expression of Fas, transforming growth factor beta1 (TGFbeta1) and HBcAg in the liver tissues were detected by immunohistochemistry, and DNA fragmentation was examined by TUNEL assay. The levels of the serum markers for liver fibrosis and liver function were also measured.Results: Patients with liver fibrosis in S3-S4 stages had high pathological expression of Fas and TGFbeta1 with severe DNA damage in the liver tissues. After 3 months of interferon therapy, the expression of Fas and TGFbeta1 were lowered (P<0.05), and further treatment till 3-9 months resulted in gradual decrease in the degree of hepatic fibrosis and cell apoptosis (P<0.05), with improved serum liver fibrosis indices and liver function.Conclusion: Interferon alpha may alleviate liver fibrosis and suppress cell apoptosis in patients in S3-S4 stages after a 6- to 9-month continuous treatment.","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25725878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of FLT3 gene and FLT3/ITD mutation by polymerase chain reaction-single-strand conformation polymorphism in patients with acute lymphoblastic leukemia. 聚合酶链反应-单链构象多态性检测急性淋巴细胞白血病患者FLT3基因及FLT3/ITD突变。

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-10-01

Bing Xu, Lin Li, Jia-hong Tang, Shu-yun Zhou

{"title":"Detection of FLT3 gene and FLT3/ITD mutation by polymerase chain reaction-single-strand conformation polymorphism in patients with acute lymphoblastic leukemia.","authors":"Bing Xu, Lin Li, Jia-hong Tang, Shu-yun Zhou","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To analyze Fms-like tyrosine kinase 3 (FLT3) gene and FLT3 internal tandem duplication (ITD) mutation in acute lymphoblastic leukemia (ALL) patients of different immunological subtypes.Methods: Polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) was used to detect FLT3 gene and FLT3/ITD mutation in 63 ALL cases.Results: Among the 63 ALL cases, FLT3 gene was detected in 41 (61.5%) cases. The positivity rate of FLT3 gene in pre-pre B-lineage ALL, pre-B-ALL, B-lineage ALL and T-lineage ALL cases were 93.3% (14/15), 77.8% (14/18), 41.7% (5/12) and 28.6% (4/14), respectively. The positivity rate of FLT3 gene was significantly higher in pre-pre B-ALL/pre B-ALL subtypes (84.8%) than in B-ALL subtypes (41.7%, P<0.005), and the rate was significantly higher in B-ALL subtypes (73.3%) than in T-ALL subtypes (28.6%, P<0.001). Two cases (3.2%) were found to have FLT3/ITD mutation, which were also positive for myeloid antigen expression and diagnosed as acute mixed-lineage leukemia, showing leukocytosis and high percentage of bone marrow blast cells with poor prognosis.Conclusions: FLT3 gene can be detected in both B-and T-lineage ALL patients, but more frequently in the former. In B-lineage ALL patients, FLT3 gene is more frequent in cases with undifferentiated than those with differentiated blast cells. FLT3/ITD is rarely detected in ALL patients and FLT3/ITD mutation detection might be helpful to identify the genotypes and evaluate the prognosis of acute leukemia.","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25664429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Expression of urokiase-type plasminogen activator and matrix metalloproteinases in human tissue of abdominal aortic aneurysm]. [尿酶型纤溶酶原激活剂和基质金属蛋白酶在人腹主动脉瘤组织中的表达]。

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-10-01

Yan Li, Ye-wei Zhang

引用次数: 0