Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA最新文献_第7页

[Report of two cases of intracranial subependymoma]. 颅内室管膜下瘤2例报告

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

Xi-an Zhang, Song-tao Qi, Yu-ping Peng

引用次数: 0

[Preparation and identification of monoclonal antibodies against different epitopes on human zeta globin chain]. [人zeta球蛋白链不同表位单克隆抗体的制备与鉴定]。

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

Lei Tang, Ping Zhu, Chen Luo, Xiang-ming Xu, Ning Fu

{"title":"[Preparation and identification of monoclonal antibodies against different epitopes on human zeta globin chain].","authors":"Lei Tang, Ping Zhu, Chen Luo, Xiang-ming Xu, Ning Fu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To prepare and identify the monoclonal antibodies (mAb) against human zeta globin chain.Methods: BALB/c mice were immunized with purified recombinant zeta globin chain, and the hybridomas were generated by fusion of mouse spleen cells and myeloma cells NS-1. After three fusions and successive cloning, 3 hybridoma cell lines secreting the mAb against zeta were obtained and the antibodies were purified from the ascites, followed by identification with indirect enzyme-linked immunosorbent assay (ELISA) and sandwich ELISA in combination with rabbit anti-zeta serum.Results and conclusion: Three hybridoma cell lines secreting anti-zeta mAb were established, designated as 1A12, 3H9 and 4D11, respectively. Both of 3H9 and 4D11 mAbs belonged to IgG1 isotype and mAb 1A12 to IgG2a. All the mAbs could bind specifically to recombinant zeta and natural zeta globin from hemolysate of --(SEA) gene carrier. In addition, 1A12 and 3H9 mAbs could recognize different epitopes on zeta globin, suggesting the possibility of developing a detection system for screening alpha-thalassemia with these mAbs.","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":"25 11","pages":"1394-7"},"PeriodicalIF":0.0,"publicationDate":"2005-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25725874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Low field magnetic resonance imaging manifestations of toxic encephalopathy induced by heroin inhalation: report of 3 cases]. 海洛因吸入致中毒性脑病的低场磁共振成像表现(附3例报告)。

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

Yi Xu, Hong Gu

引用次数: 0

[Construction of standard recombinant plasmids for 7 common mannan-binding lectin gene haplotypes]. 7种常见甘露聚糖结合凝集素基因单倍型标准重组质粒的构建

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

Xin-pei Yu, Yue Chen, Li-yun Zhang, Xiao Lu, Zheng-liang Chen

{"title":"[Construction of standard recombinant plasmids for 7 common mannan-binding lectin gene haplotypes].","authors":"Xin-pei Yu, Yue Chen, Li-yun Zhang, Xiao Lu, Zheng-liang Chen","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To construct the standard recombinant plasmids for 7 common haplotypes of mannan-binding lectin (MBL) gene.Methods: The DNA samples with known haplotypes and genotypes of MBL gene were used as the templates for amplifying the fragments of MBL gene haplotypes including the promoter region and exon 1 with sequence-specific primer-polymerase chain reaction (SSP-PCR) method. The amplified fragments were cloned into T vector and the bases located at codon 52 and codon 57 of exon 1 in MBL gene were mutated respectively by site-directed mutagenesis. All the 7 recombinant plasmids were identified by PCR and direct sequence analysis.Results: From the DNA samples with known haplotypes and genotypes of MBL gene, the standard plasmids of haplotypes HYPA, LXPA, LYQA, LYPA and LYPB of MBL gene were constructed by SSP-PCR and molecular cloning technique. From the recombinant plasmids of HYPA and LYQA, the standard plasmids of haplotypes HYPD and LYQC of MBL gene were constructed by site-directed mutagenesis, respectively.Conclusion: The constructed standard plasmids of haplotypes HYPA, LXPA, LYQA, LYPA, LYPB, HYPD and LYQC of MBL gene provide standard controls for detecting the SNPs, haplotypes and genotypes of MBL gene with such genotyping methods us SSP-PCR and real-time PCR.","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":"25 11","pages":"1379-83"},"PeriodicalIF":0.0,"publicationDate":"2005-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25705733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Construction of eukaryotic expression vectors of two mutants of hypoxia-inducible factor-1 and their expressions in human microvascular endothelial cells]. [两种缺氧诱导因子-1突变体真核表达载体的构建及其在人微血管内皮细胞中的表达]。

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

Rui-bin Fu, Ping-sheng Wu, Yun-feng Song, Jian Qiu, Tie-ying Dai, Jian-hua Li, Jian-cheng Xiu

{"title":"[Construction of eukaryotic expression vectors of two mutants of hypoxia-inducible factor-1 and their expressions in human microvascular endothelial cells].","authors":"Rui-bin Fu, Ping-sheng Wu, Yun-feng Song, Jian Qiu, Tie-ying Dai, Jian-hua Li, Jian-cheng Xiu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To construct eukaryotic expression vectors of two mutants of hypoxia-inducible factor-1 (HIF-1alpha) and study their expressions in human microvascular endothelial cells (HMVECs).Methods: Site-directed mutagenesis was performed to induce the mutation of the codons for the residue Pro564 (ccc) in HIF-1alpha into gcc (Ala) in pcDNA3.1(+)-HIF-1alphato obtain single-site-mutated vector pcDNA3.1(+)-HIF-1alpha-564Ala, which was then subjected to a second site-directed mutagenesis to convert the codons for Asn803 into that of Ala (gct) to acquire double-site-mutated pcDNA3.1(+)-HIF-1alpha-564Ala-803Ala. After lipofectin-mediated transient transformation of HMVECs with the 3 recombinant plasmids including the two plasmids containing the mutations and the one without mutation, respectively, the expression levels of HIF-1alpha mRNA and protein were determined using RT-PCR, immunofluorescent staining and Western blotting.Results: DNA sequence analysis demonstrated success of the two-step mutagenesis and the two plasmids of pcDNA3.1+-HIF-1alpha-564Ala and pcDNA3.1(+)-HIF-1alpha-564Ala- 803Ala were obtained, both of which could produce HIF-1alpha protein resistant to oxidation degradation in HMVECs as compared with the non-mutated one.Conclusion: The recombinant plasmids pcDNA3.1(+)-HIF-1alpha-564Ala and pcDNA3.1(+)-HIF-1alpha- 564Ala-803Ala have been successfully constructed with efficient expressions in HMVECs.","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":"25 11","pages":"1348-51"},"PeriodicalIF":0.0,"publicationDate":"2005-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25706378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Effects of local carbon ion irradiation on survival and peripheral blood leukocyte and platelet counts of tumor-bearing mice]. 局部碳离子照射对荷瘤小鼠存活及外周血白细胞和血小板计数的影响。

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

Xiao-fan Ding, Long-hua Chen, Wen-jian Li, De-xing Li

{"title":"[Effects of local carbon ion irradiation on survival and peripheral blood leukocyte and platelet counts of tumor-bearing mice].","authors":"Xiao-fan Ding, Long-hua Chen, Wen-jian Li, De-xing Li","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the effects of local carbon ion irradiation on the length of survival and peripheral blood leukocyte and platelet counts of mice inoculated with pulmonary tumor cells.Methods: Thirty tumor-bearing mice were randomly divided into control group (tumor-bearing but without carbon ion irradiation, n=10), 12 Gy group (n=10) and 24 Gy group (n=10). The right hind limbs of the mice, where the tumor cells were inoculated, were irradiated with carbon-ion beams at a single dose in 12 Gy and 24 Gy groups, and those of the control group received no irradiation. The peripheral blood leukocytes and platelets of the mice in all the 3 groups were counted immediately before and 7 and 14 days after irradiation, respectively, with the survival time of the mice recorded.Results: There was no significant difference in the survival time of the mice between the 3 groups. The peripheral blood leukocyte counts in all groups increased after irradiation, no significant difference was noted between the two irradiation the groups and the control group. Irradiation at 24 Gy resulted in significant increase in peripheral blood platelet count on the 14th day (P=0.032, F=4.062), but no such increment was observed in the other 2 groups or on day 7 in the 24 Gy group.Conclusion: Local carbon ion irradiation may not produce significant effects on the length of survival and hemogram of the tumor-bearing mice.","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":"25 11","pages":"1432-4"},"PeriodicalIF":0.0,"publicationDate":"2005-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25716611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Oxidative stress-induced differentiation of L6 myoblasts]. 氧化应激诱导的L6成肌细胞分化。

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

Xiao-zhong Qiu, Lei Yu, Hua Liao, Li-sheng Zhang, Jian-qiang Qin, Jun Yang, Jun Ouyang

引用次数: 0

[Construction and characterization of mutant Enteroaggregative E. coli O42 strain with high-pathogenicity island deletion]. [高致病性岛型缺失肠聚集性大肠杆菌O42突变株的构建与鉴定]。

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

Jing Hu, Shou-yi Yu, Biao Kan, Zhi-hua Liu

引用次数: 0

[Differentiation of neonatal rat striatal neural stem cells induced by all-trans retinoic acid]. [全反式维甲酸诱导新生大鼠纹状体神经干细胞分化]。

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

Hong Deng, Fei Zou, Hai-ji Luo

引用次数: 0

[Expression of cathepsins B and L in early gestational decidua and chorionic villi]. 组织蛋白酶B和L在妊娠早期蜕膜和绒毛膜绒毛中的表达

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

An-ming Wang, Shi-ling Chen, Fu-qi Xing

{"title":"[Expression of cathepsins B and L in early gestational decidua and chorionic villi].","authors":"An-ming Wang, Shi-ling Chen, Fu-qi Xing","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To study the expression of cathepsins B and L in first-trimester gestational decidua and chorionic villi.Methods: The decidua and chorionic villi in the first trimester of gestation were obtained from 30 women undergoing induced abortion, 25 with spontaneous abortion, 10 with normal endometrium in secretory phase, and 15 with bydatidiform mole in whom the expression of cathepsins B and L was determined by immunohistochemistry.Results: Positive staining for cathepsins B and L were mainly detected in the trophoblasts and decidual cells. The positive expression rate of cathepsin B in the normal endometrium of secretory phase, decidua in the first trimester of gestation and spontaneous abortion were 10% (1/10), 83.3% (25/30), and 32.0% (8/25), respectively, and the rate of cathepsin L expression in the endometrium in secretory phase, decidua of induced abortion and spontaneous abortion were 0.0%, 63.3% (19/30), and 32.0% (8/25) respectively, showing significant difference in the expression rates of cathepsins B and L between the 3 groups (P<0.05). Strong positive expression of cathepsin B in chorionic villi of induced abortion, spontaneous abortion and hydatidiform mole were detected at the rates of 10.0% (3/30), 0.0%, and 66.7% (10/15), respectively, and cathepsin L at the rates of 26.7% (8/30), 4.0% (1/25), and 80.0% (12/15), respectively, with significant difference in strong cathepsins B and L expressions between the 3 groups (P<0.05).Conclusion: The expression of cathepsins B and L differs in normal and abnormal deciduas and chorionic villi of early pregnancy, suggesting that the cathepsins may play important roles in the process of implantation.","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":"25 11","pages":"1365-8"},"PeriodicalIF":0.0,"publicationDate":"2005-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25705730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0