发布求助
文献互助 智能选刊 最新文献

Cytobios最新文献

筛选
英文 中文
The novel peptide composition of the seeds of Trichosanthes dioica Roxb. 薯蓣种子新肽组成的研究。
Cytobios Pub Date : 2000-01-01
S Kabir
{"title":"The novel peptide composition of the seeds of Trichosanthes dioica Roxb.","authors":"S Kabir","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The seeds of Trichosanthes dioica contain a large amount of peptides in the range of 2-8 kD. These peptides can be resolved in a discontinuous sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) system using Tricine as the trailing ion. The seed proteins contain a number of charge species as determined by isoelectric focusing (IEF) in polyacrylamide gels. The peptides were focused in the basic region as determined by two-dimensional electrophoresis involving IEF and SDS-PAGE. The seed peptides have the unique property of being resistant to the action of silver nitrate, a sensitive reagent commonly used to stain proteins. The seed contains haemagglutinating activity which is inhibited by galactose.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":"103 403","pages":"121-31"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21902999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Male reproductive toxicity of vincristine: ultrastructural changes in the epididymal epithelial apical cell. 长春新碱的雄性生殖毒性:附睾上皮顶端细胞的超微结构变化。
Cytobios Pub Date : 2000-01-01
M A Akbarsha, H I Averal, R Girija, S Anandhi, A Faridha Banu
{"title":"Male reproductive toxicity of vincristine: ultrastructural changes in the epididymal epithelial apical cell.","authors":"M A Akbarsha,&nbsp;H I Averal,&nbsp;R Girija,&nbsp;S Anandhi,&nbsp;A Faridha Banu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The toxic effect of vincristine on the apical cells of the rat caput epididymis was investigated. The drug was administered at 20 and 40 microg/kg body weight daily for 15 days. Light microscopy using semithin sections, and transmission electron microscopy, of the caput epididymis were undertaken. The results revealed that the basal region of the apical cell was in contact with the basement membrane and the luminal end took part in endocytosis. The apical cells reflected a dose-dependent response to vincristine (VCR) treatment. In general the changes included protrusion of the apical ends deep into the lumen, with the nucleus of the cell located in such protruded ends, and an increase in the abundance of lysosomal bodies and multivesicular bodies. These changes reflected the physiological response of the apical cell to VCR treatment rather than toxic manifestations.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":"102 400","pages":"85-93"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21727006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of luteolin on arylamine N-acetyltransferase activity in human liver tumour cells. 木犀草素对人肝肿瘤细胞芳胺n -乙酰转移酶活性的影响。
Cytobios Pub Date : 2000-01-01
J C Chen, J G Chung, K M Lin
{"title":"Effects of luteolin on arylamine N-acetyltransferase activity in human liver tumour cells.","authors":"J C Chen,&nbsp;J G Chung,&nbsp;K M Lin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The human liver tumour cell line (J5) was selected in order to evaluate whether or not luteolin affected arylamine N-acetyltransferase (NAT) activity. Using high performance liquid chromatography, the NAT activity for acetylation of arylamine substrates (2-aminofluorene and p-aminobenzoic acid) was determined. The cytosolic NAT activity in human liver tumour cells was 2.74+/-0.26 and 1.68+/-0.20 nmol/min/mg of protein for 2-aminofluorene and p-aminobenzoic acid, respectively. Luteolin displayed a dose-dependent inhibition to cytosolic NAT activity and intact human liver tumour cells. Time-course experiments showed that NAT activity measured from intact human liver tumour cells was inhibited by luteolin for up to 24 h. Using standard steady-state kinetic analysis, it was shown that luteolin was a possible noncompetitive inhibitor to NAT activity in cytosols. This report is the first to show how luteolin affects NAT activity in human liver tumour cells.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":"102 400","pages":"95-106"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21728156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The B chromosome system of Trypoxylon (Trypargilum) albitarse (Hymenoptera, Sphecidae) 1. Banding analysis. 雌雄蜂(膜翅目，蜂科)的B染色体系统联合分析。
Cytobios Pub Date : 2000-01-01
S M Araújo, S G Pompolo, J A Dergam, L A Campos
{"title":"The B chromosome system of Trypoxylon (Trypargilum) albitarse (Hymenoptera, Sphecidae) 1. Banding analysis.","authors":"S M Araújo,&nbsp;S G Pompolo,&nbsp;J A Dergam,&nbsp;L A Campos","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Karyotypic analyses of 366 specimens of the solitary wasp Trypoxylon (Trypargilum) albitarse collected from ten populations in the municipalities of Viçosa and Porto Firme (Minas Gerais, Southeastern Brazil), revealed the presence of two morphological types of supernumerary (B) chromosomes. C-banding and fluorochrome banding suggest that the B chromosomes of T. albitarse may have originated from heterochromatin breaks within the standard (A) chromosome complement.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":"101 396","pages":"7-13"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21551164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of constitutive heterochromatin of Callithrix geoffroyi (Callitrichidae, Primates) by restriction enzymes and fluorochrome bands. 利用限制性内切酶和荧光染料条带对黄油鸡(Callithrix geoffroyi)组成异染色质的研究。
Cytobios Pub Date : 2000-01-01
J C Pieczarka, C Y Nagamachi, A Pissinatti, R M Barros, M S Mattevi
{"title":"Characterization of constitutive heterochromatin of Callithrix geoffroyi (Callitrichidae, Primates) by restriction enzymes and fluorochrome bands.","authors":"J C Pieczarka,&nbsp;C Y Nagamachi,&nbsp;A Pissinatti,&nbsp;R M Barros,&nbsp;M S Mattevi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The neotropical primate genus Callithrix comprises two groups of species, jacchus and argentata, which inhabit distinct geographical regions and manifest different fur coloration and constitutive heterochromatin (CH) markers in their karyotypes. In this investigation the CH of a representative of the jacchus group, Callithrix geoffroyi, was analysed using fluorochromes and restriction enzymes in situ. To clarify the source of the constitutive heterochromatin of both groups, the data obtained in the jacchus group were compared with those published in the argentata group obtained by the same techniques. The C-bands of C. geoffroyi (four specimens, 2n = 46) were centromeric in all chromosomes, and distally located in pairs 6 and 22. The Alu I, Hae III, Hin fI, Rsa I, Dde I, Mbo I, and Msp I restriction endonucleases and CMA3 and DAPI fluorochromes produced different bands, which allowed the characterization of four distinct types of constitutive heterochromatin in the C. geoffroyi genome. Several of these types of heterochromatin were present in the ancestor of the two groups of species, jacchus and argentata, while others originated after their cladogenesis.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":"101 398","pages":"161-72"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21605266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Female breast carcinomas: nuclear and cytoplasmic proteins versus steroid receptors. 女性乳腺癌:核和细胞质蛋白与类固醇受体。
Cytobios Pub Date : 2000-01-01
M Bryś, H Romanowicz-Makowska, A Nawrocka, W M Krajewska
{"title":"Female breast carcinomas: nuclear and cytoplasmic proteins versus steroid receptors.","authors":"M Bryś,&nbsp;H Romanowicz-Makowska,&nbsp;A Nawrocka,&nbsp;W M Krajewska","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Nuclear and cytoplasmic proteins of human female breast cancer were analysed by one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Oestrogen receptor and progesterone receptor expression was determined by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) method. The electropherograms were developed by silver nitrate staining and quantitative analysis was carried out by video densitometer using the software Gel-Pro Analyzer. Nuclear and cytoplasmic proteins of breast carcinomas and normal tissue differed both qualitatively and quantitatively. Nuclear polypeptides of 108, 53 and 48 kD as well as the 36 kD cytoplasmic polypeptide were specific for tumour samples, while the 51 kD nuclear polypeptide was detected only in normal tissue. Quantitative differences in band density were noted in the 32 kD nuclear polypeptide. This polypeptide was expressed in greatest concentration in infiltrating ductal carcinomas which also indicated the greatest oestrogen receptor gene expression. This relationship appeared to be statistically significant (p < 0.005). No correlations were evident between the 32 kD protein expression and the progesterone receptor gene expression in any of the tissue types examined, nor between the 32 kD protein and the patient's age or tumour grade.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":"101 397","pages":"87-94"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21607767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
DNA degradation in the kidney of folic acid-treated guinea pigs. 叶酸处理豚鼠肾脏的DNA降解。
Cytobios Pub Date : 2000-01-01
P M Zeis, M Tzaki, L Nakopoulou, P Nicolaidou, E Kavazarakis, A Messaritaki, M Moustaki, M P Zeis, D Gourgiotis
{"title":"DNA degradation in the kidney of folic acid-treated guinea pigs.","authors":"P M Zeis,&nbsp;M Tzaki,&nbsp;L Nakopoulou,&nbsp;P Nicolaidou,&nbsp;E Kavazarakis,&nbsp;A Messaritaki,&nbsp;M Moustaki,&nbsp;M P Zeis,&nbsp;D Gourgiotis","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Previous investigators agree on the increased DNA synthesis and destruction of tissues caused by folic acid (FA) administered parenterally. This study aims to clarify whether DNA degradation due to the destruction of cells and nuclei precedes DNA synthesis following FA administration. Forty guinea pigs were divided into four groups: group 1, contained 10 controls; in group 2, ten animals received intraperitoneally 300 mg/kg of body wt FA; in group 3, ten animals received FA and 12 h later frusemide intramuscularly in a dose of 7 mg/kg body wt; and finally in group 4, ten animals received frusemide as in group 3. FA produced necrosis of the epithelial cells of the convoluted tubules as the detection of the beta-aminoisobutyric acid end product of DNA and thymine catabolism indicated. Frusemide administered in group 3 had a favourable effect on the acute renal failure induced by FA.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":"102 400","pages":"107-13"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21728157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chromosome evolution involving Robertsonian rearrangements in Xyrichthys fish (Labridae, Perciformes). 包含Robertsonian重排的Xyrichthys鱼的染色体进化(唇形科)。
Cytobios Pub Date : 2000-01-01
K Ueno, A Takai
{"title":"Chromosome evolution involving Robertsonian rearrangements in Xyrichthys fish (Labridae, Perciformes).","authors":"K Ueno,&nbsp;A Takai","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Three Xyrichthys fish (Labridae, Perciformes), X. pavo, X. dea, and X. twistii, were cytogenetically studied. X. pavo and X. dea had 2n = 44 chromosomes, which were all acrocentric. X. twistii had 2n = 22 chromosomes consisting of eighteen meta- and submetacentric and four acrocentric chromosomes. The cellular DNA contents of X. pavo and X. twistii measured using flow cytometry were nearly equal. These results suggest that the karyotype of X. twistii evolved by decreasing the number of chromosomes by fusion events, probably Robertsonian fusion. Cytogenetic relationships among the three species were surmized on the basis of features on the karyotypes and the NOR locations. A large gap in the chromosome number between 2n = 44 and 2n = 22 is an interesting feature related to the process of chromosome evolution.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":"103 402","pages":"7-15"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21862264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The sequestration of hydroxyl ions by CO2 in liquid water: the physiological implications and the second function of carbonic anhydrase. 液态水中CO2对羟基离子的固存:碳酸酐酶的生理意义和第二功能。
Cytobios Pub Date : 2000-01-01
W F Widdas, G F Baker
{"title":"The sequestration of hydroxyl ions by CO2 in liquid water: the physiological implications and the second function of carbonic anhydrase.","authors":"W F Widdas,&nbsp;G F Baker","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The pH changes due to bubbling CO2 through water produced anomalies which were more readily explained by an hypothesis based on electrostatic attractions between the molecules. The present studies have suggested that an hexagonal array of six carbon dioxide molecules could bind and sequester a hydroxyl anion. The binding energy of the complex is estimated to be comparable with that of a covalent compound and its dissociation may only occur at the water interface with air or at the water/hydrophobic protein interface in a protein cleft. The physiological importance lies in the consequential release of an equal number of free hydrogen ions (H3O+) and the disruption of the normal action of buffer systems in regulating the cytoplasmic pH. The counteraction of this sequestration reaction and the acid-base disturbances which result, form the second important function of carbonic anhydrase isoforms, the mechanisms of which are briefly discussed.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":"103 402","pages":"39-60"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21862267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Extracellular matrix effects on a neuroblastoma cell line. 细胞外基质对神经母细胞瘤细胞系的影响。
Cytobios Pub Date : 2000-01-01
M Hahn, T Glass, J Koke
{"title":"Extracellular matrix effects on a neuroblastoma cell line.","authors":"M Hahn,&nbsp;T Glass,&nbsp;J Koke","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cells of various lines assume similar shapes when grown attached to substrates like coverslips. In contrast, cells cultured in a collagen and/or laminin matrix often assume a relatively normal morphology in comparison with their in situ counterparts. During investigations of neuroblastoma SH-SY5Y cells, an attempt was made to identify culture conditions which would cause the cells to assume a more regular shape. SH-SY5Y cells cultured on bare coverslips, on coverslips coated with rat-tail collagen, and in approximately 1 mm thick gels containing extracellular matrix components were compared. Striking differences were apparent when comparing the gel-cultured cells with cells cultured on coverslips. Cells grown in the gel formed ganglia-like clusters which generated bundles of neurites which targeted other 'ganglia'. The same cells grown on coverslips, whether or not they were collagen-coated, appeared unaware of the presence of other cells, and did not cluster, nor did they generate neurites.</p>","PeriodicalId":11078,"journal":{"name":"Cytobios","volume":"102 399","pages":"7-19"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21667782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：604180095
Book学术官方微信
小红书