Current stem cell research & therapy最新文献_第9页

Roles of Stem Cell Exosomes and their MicroRNA Carrier in Bone and Cartilage Regeneration. 干细胞外泌体及其MicroRNA载体在骨和软骨再生中的作用。

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2023-01-01 DOI: 10.2174/1574888X17666220817093305

Dazhi Yang, Zecai Chen, Zhen Xu, Yufeng Long, Lei Qin, Weihong Yi

{"title":"Roles of Stem Cell Exosomes and their MicroRNA Carrier in Bone and Cartilage Regeneration.","authors":"Dazhi Yang, Zecai Chen, Zhen Xu, Yufeng Long, Lei Qin, Weihong Yi","doi":"10.2174/1574888X17666220817093305","DOIUrl":"https://doi.org/10.2174/1574888X17666220817093305","url":null,"abstract":"Bone and cartilage regeneration is a dynamic and complex process involving multiple cell types, such as osteoblasts, osteoclasts, endothelial cells, etc. Stem cells have been proved to have an efficient capability to promote bone and cartilage regeneration and repair, but the usage of cells harbors some important safety issues, such as immune rejection and carcinogenicity. Exosomes are non-cell structures secreted from various cells. The content of exosomes is enriched with proteins, such as cytoskeleton proteins, adhesion factors, transcription factors, etc., and a variety of nucleic acids, such as mRNA (Messenger RNA), long-chain non-coding RNA, microRNA (miRNA), etc. Exosomes can deliver a variety of contents from the parent cells to the recipient cells in different tissue backgrounds, influencing the phenotype and function of the recipient cells. Recent studies have demonstrated that miRNAs play significant roles in bone formation, suggesting that miRNAs may be novel therapeutic targets for bone and cartilage diseases. Exosomes have been shown with low/no immune rejection in vivo, no carcinogenic risk of infection, nor other side effects. In recent years, stem cell exosomes have been utilized to promote bone and cartilage regeneration processes during bone defect, bone fracture, cartilage repair, osteoporosis, and osteoarthritis. In this review, we discuss different exosomes derived from stem cells and their interactions with target cells, including osteoblasts, chondrocytes and osteoclasts. We also highlight the various signaling pathways involved in stem cell exosome-related bone and cartilage regeneration.","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 7","pages":"917-925"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9570397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Induced Pluripotent Stem Cells in Moyamoya Disease: Progress and Promises. 诱导多能干细胞在烟雾病中的应用:进展与展望。

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2023-01-01 DOI: 10.2174/1574888X17666220607121027

Yimeng Xue, Qian Zhang, Lin-Jian Wang, Wen-Jun Tu, Jizong Zhao

引用次数: 1

Induction of Caspase-dependent Apoptosis in Rat Bone Marrow Mesenchymal Stem Cells Due to Di-2-Ethylhexyl Phthalate Toxicity was Found to Arrest the Cell Cycle at the G1 Stage. 邻苯二甲酸二-2-乙基己基酯毒性诱导大鼠骨髓间充质干细胞caspase依赖性凋亡可使细胞周期阻滞在G1期。

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2023-01-01 DOI: 10.2174/1574888X18666230106114727

Abnosi Mohammad Hussein, Sargolzaei Javad, Shayeganfar Zahra

{"title":"Induction of Caspase-dependent Apoptosis in Rat Bone Marrow Mesenchymal Stem Cells Due to Di-2-Ethylhexyl Phthalate Toxicity was Found to Arrest the Cell Cycle at the G1 Stage.","authors":"Abnosi Mohammad Hussein, Sargolzaei Javad, Shayeganfar Zahra","doi":"10.2174/1574888X18666230106114727","DOIUrl":"https://doi.org/10.2174/1574888X18666230106114727","url":null,"abstract":"Background: Di-(2-ethylhexyl) phthalate (DEHP) is used as a plasticizer in polyvinyl chloride products which is widely utilized. Previously we found, DEHP reduced the viability and proliferation ability of bone marrow mesenchymal stem cells (BMSCs).Objective: In the present study, the mechanism of DEHP toxicity was investigated.Methods: Rat BMSCs were cultured up to 3rd passage and their viability was determined after treatment with 100 and 500 μM of DEHP for 24 and 48 hours. The levels of sodium, potassium, and calcium as well as induction of apoptosis were investigated. Using flow cytometry, cell cycle analysis was performed and the expression of genes involved in the cell cycle was evaluated using reverse transcriptase-PCR. Data were analyzed and p < 0.05 was taken as the level of significance.Results: Although the viability and electrolyte level of BMSCs were not affected with 100 μM of DEHP, this environmental pollution induced caspase-dependent apoptosis in a concentration-dependent manner. In both of the concentrations, DEHP arrests the cell cycle at the G0/G1 phase, and the expression of Cdk2 and Cdk4 was significantly reduced whereas an over-expression of P53 was observed. However, the expression of the raf1 gene remained unchanged.Conclusion: DEHP induces caspase-dependent apoptosis in BMSCs and arrests the cell cycle due to the reduction of Cdk2 and Cdk4 expression via over-expression of P53.","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 8","pages":"1106-1112"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9842108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amniotic Fluid Stem Cells: What They Are and What They Can Become. 羊水干细胞:它们是什么以及它们能成为什么。

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2023-01-01 DOI: 10.2174/1574888X16666211210143640

Margit Rosner, Markus Hengstschläger

{"title":"Amniotic Fluid Stem Cells: What They Are and What They Can Become.","authors":"Margit Rosner, Markus Hengstschläger","doi":"10.2174/1574888X16666211210143640","DOIUrl":"https://doi.org/10.2174/1574888X16666211210143640","url":null,"abstract":"In the last two decades, fetal amniotic fluid stem cells progressively attracted attention in the context of both basic research and the development of innovative therapeutic concepts. They exhibit broadly multipotent plasticity with the ability to differentiate into cells of all three embryonic germ layers and low immunogenicity. They are convenient to maintain, highly proliferative, genomically stable, non-tumorigenic, perfectly amenable to genetic modifications, and do not raise ethical concerns. However, it is important to note that among the various fetal amniotic fluid cells, only c-Kit+ amniotic fluid stem cells represent a distinct entity showing the full spectrum of these features. Since amniotic fluid additionally contains numerous terminally differentiated cells and progenitor cells with more limited differentiation potentials, it is of highest relevance to always precisely describe the isolation procedure and characteristics of the used amniotic fluid-derived cell type. It is of obvious interest for scientists, clinicians, and patients alike to be able to rely on up-todate and concisely separated pictures of the utilities as well as the limitations of terminally differentiated amniotic fluid cells, amniotic fluid-derived progenitor cells, and c-Kit+ amniotic fluid stem cells, to drive these distinct cellular models towards as many individual clinical applications as possible.","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 1","pages":"7-16"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10858223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The Role of m6A in Osteoporosis and the Differentiation of Mesenchymal Stem Cells into Osteoblasts and Adipocytes. m6A在骨质疏松和间充质干细胞向成骨细胞和脂肪细胞分化中的作用。

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2023-01-01 DOI: 10.2174/1574888X17666220621155341

Weifei Zhang, Tao Ke, Jianjing Lin, Peng Liu, Zhiping Guan, Jiapeng Deng, Deli Wang, Hui Zeng

引用次数: 3

Human Amniotic Fluid Stem Cells Exert Immunosuppressive Effects on T Lymphocytes in Allergic Rhinitis. 人羊水干细胞对变应性鼻炎患者T淋巴细胞的免疫抑制作用

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2023-01-01 DOI: 10.2174/1574888X17666220926105744

Ling Zong, De Wang, Yanbo Long, Xiaolan Liu, Ailin Tao, Lanzhen Zhang, Jinming Zhai

{"title":"Human Amniotic Fluid Stem Cells Exert Immunosuppressive Effects on T Lymphocytes in Allergic Rhinitis.","authors":"Ling Zong, De Wang, Yanbo Long, Xiaolan Liu, Ailin Tao, Lanzhen Zhang, Jinming Zhai","doi":"10.2174/1574888X17666220926105744","DOIUrl":"https://doi.org/10.2174/1574888X17666220926105744","url":null,"abstract":"Aim: The study aims to investigate the immunomodulatory effect of Amniotic fluid stem (AFS) cells to Th2-skewed allergic rhinitis (AR) on T-lymphocyte proliferation, viability, activation and cytokine production.Background: AFS cells can suppress peripheral blood mononuclear cells (PBMCs) proliferation and display immunomodulatory properties, but AFS cells' immunoregulation on AR has not been defined.Methods: Human AFS cells were derived from magnetic cell sorting and co-cultured with PBMCs from AR patients stimulated by phytohemagglutinin (PHA). The AFS cells-associated suppressive proliferation was analyzed using CellTrace™ Violet assay; the T lymphocytes proliferation, viability, activation and the Foxp3+ Treg cells were determined by flow cytometry; cytokine levels were measured using an enzyme- linked immunosorbent assay.Results: We determined that AFS cells significantly inhibited PHA-induced CD3+ T lymphocyte proliferation at the ratio higher than 1:50 (AFS cells: PBMCs) (P<0.05); AFS cells obviously increased the T lymphocytes viability (P<0.01), inhibited the apoptosis of T lymphocytes (P<0.001), compared to PBMCs alone; AFS cells suppressed CD3+CD25+ T lymphocytes activated by PHA (P<0.05); AFS cells significantly promote Treg cells expansion in house dust mite (HDM)-stimulated PBMCs from AR patients (P<0.05). Compared with HDM-stimulated PBMCs, AFS cell co-culture predominantly decreased IL-4 level (P<0.05), but increased IFN-γ and IL-10 levels (P<0.01).Conclusion: AFS cells modulate the T-cells' immune imbalance towards Th2 suppression in AR, which can be used as a new cell banking for allergic airway diseases.","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 8","pages":"1113-1119"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9840540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cancer Stem Cells and Anti-tumor Immunity. 肿瘤干细胞与抗肿瘤免疫。

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2023-01-01 DOI: 10.2174/1574888X18666221017142032

Merve Yılmaz, Fuat Kaplan, Ilgen Mender, Sergei M Gryaznov, Z Gunnur Dikmen

{"title":"Cancer Stem Cells and Anti-tumor Immunity.","authors":"Merve Yılmaz, Fuat Kaplan, Ilgen Mender, Sergei M Gryaznov, Z Gunnur Dikmen","doi":"10.2174/1574888X18666221017142032","DOIUrl":"https://doi.org/10.2174/1574888X18666221017142032","url":null,"abstract":"Cancer stem cells (CSCs) are correlated with poor clinical outcomes due to their contribution to chemotherapy resistance and the formation of metastasis. Multiple cell surface and enzymatic markers have been characterized to identify CSCs, which is important for diagnosis, therapy, and prognosis. This review underlines the role of CSCs and circulating tumor cells (CTCs) in tumor relapse and metastasis, the characteristics of CSC and CTC biomarkers, and the techniques used to detect these cells. We also summarized novel therapeutic approaches toward targeting CSCs, especially focusing on the role of immune checkpoint blockades (ICB), such as anti-programmed death 1 (anti-PD1) and antiprogrammed death ligand-1 (anti-PDL1) therapies. Additionally, we address an intriguing new mechanism of action for small molecular drugs, such as telomere-targeted therapy 6-thio-2'deoxyguanosine (6- thio-dG), and how it reshapes tumor microenvironment to overcome ICB resistance. There are indications, that personalized cancer therapy targeting CSC populations in conjunction with immune-mediated strategy hold promise for the removal of residual therapy-resistant CSCs in the near future.","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 4","pages":"445-459"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9863903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

HOXA10 Expressing UCMSCs Transplantation Improved Endometrial Receptivity on Endometrial Injury. 表达HOXA10的UCMSCs移植可改善子宫内膜损伤后的子宫内膜容受性。

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2023-01-01 DOI: 10.2174/1574888X17666220919111814

Meixian Wu, Yuanyuan Li, Yiwei Wang, Yifan Li, Jinghui Li, Shuang Zhao, Lihua Sun, Jing Xie

{"title":"HOXA10 Expressing UCMSCs Transplantation Improved Endometrial Receptivity on Endometrial Injury.","authors":"Meixian Wu, Yuanyuan Li, Yiwei Wang, Yifan Li, Jinghui Li, Shuang Zhao, Lihua Sun, Jing Xie","doi":"10.2174/1574888X17666220919111814","DOIUrl":"https://doi.org/10.2174/1574888X17666220919111814","url":null,"abstract":"Background: Endometrial injury is considered the major cause of female infertility. Traditional therapies such as estrogen substitution therapy are not satisfactory due to individual variation in response to treatment, thereby warranting the use of alternative strategies such as stem cell therapy. Transplantation of stem cells, such as umbilical cord mesenchymal stem cells (UCMSCs), has been shown to improve endometrial healing. However, due to the effect of the intrauterine environment, the therapeutic effect of UCMSCs is limited, and its efficacy is unstable. HOXA10, encoded by the HOXA10 gene, plays an important role in endometrium morphology maintenance, proliferation, differentiation, and embryo implantation. Moreover, UCMSCs do not show HOXA10 expression.Objective: Our study aimed to evaluate the therapeutic effects of HOXA10-transfected UCMSCs on endometrial injury repair in vivo.Methods: First, we established T10-UCMSCs (UCMSCs transfected with HOXA10) for transplantation. To establish the endometrial injury model, we injected 95% ethanol into the uterine cavity and transplanted T10-UCMSCs into the uterine cavity from the cornua uteri. Fourteen days later, uteri were collected for histological and biochemical analysis of endometrial growth and receptivity.Results: Our results showed the endometrial receptivity was better in T10-UCMSCs group than in UCMSCs group, suggesting that HOXA10 could enhance the repairing ability of UCMSCs in the endometrium injury repair. More importantly, the fertility test showed that more embryos were implanted in the T10- UCMSCs group.Conclusion: Our results suggest that UCMSCs with HOXA10 expression could improve the therapeutic effects on endometrial injury repairing.","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 7","pages":"1001-1012"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10278240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10021136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The SDF-1/CXCR4 Signaling Pathway Directs the Migration of Systemically Transplanted Bone Marrow Mesenchymal Stem Cells Towards the Lesion Site in a Rat Model of Spinal Cord Injury. 在脊髓损伤大鼠模型中，SDF-1/CXCR4信号通路指导全身移植骨髓间充质干细胞向损伤部位的迁移

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2023-01-01 DOI: 10.2174/1574888X17666220510163245

Andong Zhao, Manhon Chung, Yi Yang, Xiaohua Pan, Yu Pan, Sa Cai

{"title":"The SDF-1/CXCR4 Signaling Pathway Directs the Migration of Systemically Transplanted Bone Marrow Mesenchymal Stem Cells Towards the Lesion Site in a Rat Model of Spinal Cord Injury.","authors":"Andong Zhao, Manhon Chung, Yi Yang, Xiaohua Pan, Yu Pan, Sa Cai","doi":"10.2174/1574888X17666220510163245","DOIUrl":"https://doi.org/10.2174/1574888X17666220510163245","url":null,"abstract":"Background: It has been observed that bone marrow-derived mesenchymal stem cells (MSCs) migrate towards the injured spinal cord and promote functional recovery when systemically transplanted into the traumatized spinal cord. However, the mechanisms underlying their migration to the spinal cord remain poorly understood.Methods: In this study, we systemically transplanted GFP- and luciferase-expressing MSCs into rat models of spinal cord injury and examined the role of the stromal cell-derived factor 1 (SDF-1)/CXCR4 axis in regulating the migration of transplanted MSCs to the spinal cord. After intravenous injection, MSCs migrated to the injured spinal cord where the expression of SDF-1 was increased. Spinal cord recruitment of MSCs was blocked by pre-incubation with an inhibitor of CXCR4. Their presence correlated with morphological and functional recovery. In vitro, SDF-1 or cerebrospinal fluid (CSF) collected from SCI rats promoted a dose-dependent migration of MSCs in culture, which was blocked by an inhibitor of CXCR4 or SDF-1 antibody.Results and conclusion: The study suggests that SDF-1/CXCR4 interactions recruit exogenous MSCs to injured spinal cord tissues and may enhance neural regeneration. Modulation of the homing capacity may be instrumental in harnessing the therapeutic potential of MSCs.","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 2","pages":"216-230"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9431081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Role of RNA Splicing in Regulation of Cancer Stem Cell. RNA剪接在肿瘤干细胞调控中的作用。

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2023-01-01 DOI: 10.2174/1574888X16666211207103628

Greesham Tripathi, Avantika Tripathi, Joel Johnson, Manoj Kumar Kashyap

引用次数: 5