Current stem cell research & therapy最新文献

{"title":"WITHDRAWN: Transdifferentiation of Human Neonatal Foreskin Fibroblasts into Keratinocyte-like Cells via Regulating the AKT/P53/WNT/LEF1 Axis","authors":"Hongqing Zhao, Peng Luo, Xinzhu Liu, Jiachen Sun, Zhisheng Li, Kun Zhang, Chuan'an Shen","doi":"10.2174/1574888X18666230307104051","DOIUrl":"10.2174/1574888X18666230307104051","url":null,"abstract":"<p><p>Since the authors are not responding to the editor’s requests to fulfill the editorial requirement, therefore, the article has been\u0000withdrawn from the journal Current Stem Cell Research & Therapy.</p><p><p>Bentham Science apologizes to the readers of the journal for any inconvenience this may have caused.</p><p><p>The Bentham editorial policy on article withdrawal can be found at https://benthamscience.com/pages/editorial-policies-main</p><p><strong>Bentham science disclaimer: </strong>It is a condition of publication that manuscripts submitted to this journal have not been published and will not be simultaneously\u0000submitted or published elsewhere. Furthermore, any data, illustration, structure or table that has been published\u0000elsewhere must be reported, and copyright permission for reproduction must be obtained. Plagiarism is strictly forbidden,\u0000and by submitting the article for publication the authors agree that the publishers have the legal right to take appropriate\u0000action against the authors, if plagiarism or fabricated information is discovered. By submitting a manuscript, the authors\u0000agree that the copyright of their article is transferred to the publishers if and when the article is accepted for publication.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10839296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem Cell Application for Stress Urinary Incontinence: From Bench to Bedside.","authors":"Meng Liu,&nbsp;Ying Wang,&nbsp;Guo Gao,&nbsp;Wei-Xin Zhao,&nbsp;Qiang Fu","doi":"10.2174/1574888X17666220304213057","DOIUrl":"https://doi.org/10.2174/1574888X17666220304213057","url":null,"abstract":"<p><p>Stress urinary incontinence (SUI) is a common urinary system disease worldwide. Nowadays, medical therapy and surgery can control the symptoms and improve the life quality of patients. However, they might also bring about complications as the standard therapy fails to address the underlying problem of urethral sphincter dysfunction. Recent advances in cell technology have aroused interest in the use of autologous stem cell therapy to restore the ability of urinary control. The present study reviewed several types of stem cells for the treatment of SUI in the experimental and clinical stages.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 1","pages":"17-26"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9426031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Stem Cell Therapy for Intestinal Diseases: Mechanisms, Perspectives Regarding Clinical Applications, and Challenges.","authors":"Weiwen Fan,&nbsp;Le Chang,&nbsp;Xinghua Pan,&nbsp;Xiangqing Zhu","doi":"10.2174/1574888X17666220629094253","DOIUrl":"https://doi.org/10.2174/1574888X17666220629094253","url":null,"abstract":"<p><strong>Background: </strong>Intestinal disease is a common disease, which can cause serious digestion and absorption disorders, endanger the lives of patients and seriously affect the quality of life of people. Finding an effective treatment is a difficult problem at present, and stem cell therapy as a treatment has high application potential in intestinal-related diseases.</p><p><strong>Purpose: </strong>This paper mainly summarizes the mechanism, research progress and future development trend of stem cells in the treatment of intestinal diseases in the past decade, hoping to provide a reference for future researchers in the research and application of stem cells and intestinal diseases.</p><p><strong>Methods: </strong>Stem cells, inflammatory bowel diseases, Crohn's disease, radiation-induced intestinal injury, radiation enterocolitis, and extracellular vesicles were used as search terms. Relevant references in the past ten years were searched in CNKI journal full-text database, PubMed database, VIP network and Wanfang medical network, and 80 literature studies meeting the requirements were finally included for review.</p><p><strong>Results: </strong>This paper summarizes the research and application of stem cells in intestinal diseases from 2012 to 2021, and expounds on the specific mechanism of stem cells in the treatment of intestinal diseases. It has been found that stem cells can treat intestinal injury or inflammation in different ways.</p><p><strong>Conclusion: </strong>Future stem cells may also be used to reverse the natural aging of intestinal function, improve intestinal function, and strengthen gastrointestinal function.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 4","pages":"499-512"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9485930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human Umbilical Cord Mesenchymal Stem Cells' Cultivation and Treatment of Liver Diseases.","authors":"Zihe Zhu,&nbsp;Qianqian Zhang,&nbsp;Lixin Liu,&nbsp;Jun Xu","doi":"10.2174/1574888X17666220623111406","DOIUrl":"https://doi.org/10.2174/1574888X17666220623111406","url":null,"abstract":"<p><strong>Background: </strong>Over the past few years, mesenchymal stem cells (MSCs) have been regarded as effective for treating various diseases. Among the types of MSCs, human umbilical cord mesenchymal stem cells (hUC-MSCs) have been widely studied because of their advantages in non-invasive damage to donors and the wide range of sources.</p><p><strong>Main body: </strong>This article reviews three aspects of hUC-MSCs. Foremost are the latest advances in the cultivation and preparation methods of hUC-MSCs. Furthermore, the treatments mechanism of hUCMSCs in organ transplantation and liver diseases. Finally, a summary of their use in clinical trials in liver diseases. The first part of this paper emphasizes the differences between the selection area and culture factors, including the separation method, long-term culturing in vitro, medium composition, serum, and three-dimensional (3D) skeleton system training, which could affect the characteristics of hUC-MSCs and the treatment of diseases. The second section mainly stresses the mechanisms of hUC-MSCs in the treatment of diseases, including immunoregulation and transdifferentiation into hepatocyte-like cells. Many new technologies mark and track cells in vivo and their safety. Briefly mention its role in the treatment of other diseases and vaccine preparation. In the third part, to accelerate the application of hUC-MSCs in the treatment of clinical diseases, it is necessary to expand the sample size of clinical trials to ensure their safety in the human body and determine the most effective infusion method and volume.</p><p><strong>Conclusion: </strong>hUC-MSCs have a substantial potential to become a more effective treatment for liver diseases. Clinical trials and mechanisms have laid the foundation for the normalization of clinical hUC-MSCs delivery.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 3","pages":"286-298"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9491703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview of Stem Cell Therapy in Intervertebral Disc Disease: Clinical Perspective.","authors":"Ayshe Hajiesmailpoor,&nbsp;Omid Mohamadi,&nbsp;Payam Emami,&nbsp;Gholamreza Farzanegan,&nbsp;Masoud Ghorbani","doi":"10.2174/1574888X17666220628123912","DOIUrl":"https://doi.org/10.2174/1574888X17666220628123912","url":null,"abstract":"<p><p>Intervertebral Disc Degeneration (IDD) is recognized as an aging process, an important and most common pathological condition caused by an imbalance of anabolic and catabolic metabolisms in the Intervertebral Disc (IVD), and leads to changes in the Extracellular Matrix (ECM), impaired metabolic regulation of Nucleus Pulposus (NP), and increased oxidative stress. IDD is mostly associated with pain in the back and neck, which is referred to as a type of disability. Pharmacological and surgical interventions are currently used to treat IDD, but evidence has shown that these interventions do not have the ability to inhibit the progression of IDD and restore IVD function because IVD lacks the intrinsic capacity for regeneration. Thus, therapies that rely on a degenerative cell repair mechanism may be a viable alternative strategy. Biological interventions have been assessed by attempting to regenerate IVD by restoring ECM and cellular function. Over the past decade, stem cell-based therapies have been considered, and promising results have been obtained in various studies. Given this, we reviewed clinical trials and preliminary studies of biological disc repair with a focus on stem cell therapy-based therapies.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 5","pages":"595-607"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9491707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone Marrow Mesenchymal Stem Cells Derived from Juvenile Macaques Reversed the Serum Protein Expression Profile in Aged Macaques.","authors":"Qianqian Yu,&nbsp;Chuan Tian,&nbsp;Guanke Lv,&nbsp;Qingpeng Kong,&nbsp;Gonghua Li,&nbsp;Guangxu Zhu,&nbsp;Xiangqing Zhu,&nbsp;Xinghua Pan","doi":"10.2174/1574888X17666220429111218","DOIUrl":"https://doi.org/10.2174/1574888X17666220429111218","url":null,"abstract":"<p><strong>Objective: </strong>The aim of the study was to reveal the changes in serum protein composition and content in macaques during the process of ageing, and explore the effect of bone marrow mesenchymal stem cell (BMMSC) on the serum protein expression profile in elderly macaques.</p><p><strong>Methods: </strong>Naturally ageing macaques were assessed according to age. BMMSCs were intravenously infused into aged macaques. In addition, peripheral blood was collected to obtain serum for dataindependent acquisition (DIA) protein sequencing to identify aging-related indicators. One hundred eighty days after macaques received BMMSC treatment, haemoxylin and eosin (HE) staining was performed to observe the morphology and structure of aortic arches.</p><p><strong>Results: </strong>Compared to infant and young control macaques, aged macaques showed erythema on the face, dry skin, reduced amounts of hair on the head and back, and paleness. Cultured BMMSCs from the 4th passage (P4 BMMSCs) were grown in accordance with standards used to culture mesenchymal stem cells. After BMMSC treatment, the assessed aortic arches showed no calcium salt deposition or cell necrosis, and the characteristics of the serum protein expression profile tended to be similar to that of the infant and young groups, with the expression of 41 proteins upregulated with age and that of 30 proteins downregulated with age but upregulated after BMMSC treatment. Moreover, we identified 44 significantly differentially expressed proteins between the aged model and treatment groups; 11 of the upregulated proteins were related to vascular ageing, neuronal ageing and haematopoiesis, and 33 of the downregulated proteins were associated with neuronal ageing, cardiovascular disease, and tumours. Interestingly, S100 expression in serum was significantly decreased, COMP expression was significantly increased, NKAP expression reappeared, and LCN2, CSF1R, CORO1C, CSTB and RSU-1 expression disappeared after BMMSC treatment.</p><p><strong>Conclusion: </strong>BMMSCs can reverse ageing-related serum protein expression.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 3","pages":"391-400"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9491719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive Molecule-incorporated Polymeric Electrospun Fibers for Bone Tissue Engineering.","authors":"A E Purushothaman,&nbsp;R Abhinandan,&nbsp;S Pranav Adithya,&nbsp;D Saleth Sidharthan,&nbsp;K Balagangadharan,&nbsp;N Selvamurugan","doi":"10.2174/1574888X17666220414100358","DOIUrl":"https://doi.org/10.2174/1574888X17666220414100358","url":null,"abstract":"<p><p>Bone tissue engineering (BTE) is based on the participation and combination of different biomaterials, cells, and bioactive molecules to generate biosynthetic grafts for bone regeneration. Electrospinning has been used to fabricate fibrous scaffolds, which provide nanoscale architecture comprising interconnecting pores, resembling the natural hierarchy of tissues and enabling the formation of artificial functional tissues. Electrospun fibers for BTE applications have been mostly produced from polymers (chitosan, alginate, polycaprolactone, polylactic acid) and bioceramics (hydroxyapatite). Stem cells are among the most prolific cell types employed in regenerative medicine owing to their self-renewal and differentiation capacity. Most importantly, bioactive molecules, such as synthetic drugs, growth factors, and phytocompounds, are consistently used to regulate cell behavior inducing differentiation towards the osteoblast lineage. An expanding body of literature has provided evidence that these electrospun fibers loaded with bioactive molecules support the differentiation of stem cells towards osteoblasts. Thus, this review briefly describes the current development of polymers and bioceramic-based electrospun fibers and the influence of bioactive molecules in these electrospun fibers on bone tissue regeneration.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 4","pages":"470-486"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9492605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roles of Stem Cell Exosomes and their MicroRNA Carrier in Bone and Cartilage Regeneration.","authors":"Dazhi Yang,&nbsp;Zecai Chen,&nbsp;Zhen Xu,&nbsp;Yufeng Long,&nbsp;Lei Qin,&nbsp;Weihong Yi","doi":"10.2174/1574888X17666220817093305","DOIUrl":"https://doi.org/10.2174/1574888X17666220817093305","url":null,"abstract":"<p><p>Bone and cartilage regeneration is a dynamic and complex process involving multiple cell types, such as osteoblasts, osteoclasts, endothelial cells, etc. Stem cells have been proved to have an efficient capability to promote bone and cartilage regeneration and repair, but the usage of cells harbors some important safety issues, such as immune rejection and carcinogenicity. Exosomes are non-cell structures secreted from various cells. The content of exosomes is enriched with proteins, such as cytoskeleton proteins, adhesion factors, transcription factors, etc., and a variety of nucleic acids, such as mRNA (Messenger RNA), long-chain non-coding RNA, microRNA (miRNA), etc. Exosomes can deliver a variety of contents from the parent cells to the recipient cells in different tissue backgrounds, influencing the phenotype and function of the recipient cells. Recent studies have demonstrated that miRNAs play significant roles in bone formation, suggesting that miRNAs may be novel therapeutic targets for bone and cartilage diseases. Exosomes have been shown with low/no immune rejection in vivo, no carcinogenic risk of infection, nor other side effects. In recent years, stem cell exosomes have been utilized to promote bone and cartilage regeneration processes during bone defect, bone fracture, cartilage repair, osteoporosis, and osteoarthritis. In this review, we discuss different exosomes derived from stem cells and their interactions with target cells, including osteoblasts, chondrocytes and osteoclasts. We also highlight the various signaling pathways involved in stem cell exosome-related bone and cartilage regeneration.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 7","pages":"917-925"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9570397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Induced Pluripotent Stem Cells in Moyamoya Disease: Progress and Promises.","authors":"Yimeng Xue,&nbsp;Qian Zhang,&nbsp;Lin-Jian Wang,&nbsp;Wen-Jun Tu,&nbsp;Jizong Zhao","doi":"10.2174/1574888X17666220607121027","DOIUrl":"https://doi.org/10.2174/1574888X17666220607121027","url":null,"abstract":"<p><p>Moyamoya disease (MMD) is a chronic steno-occlusion cerebrovascular disease accompanied by the formation of the abnormal vascular network at the base of the brain. The etiology of MMD is not fully clarified. Lack of pathological specimens hinders the research progress. Induced pluripotent stem cells (iPSC) derived from patients with outstanding differentiation potential and infinite proliferation ability could conquer the problem of insufficient samples. The technology of iPSC holds the promise of clarifying the underlying molecular mechanism in the development of MMD. In this review, we summarized the latest progress and difficulties in the research of mechanism and detailed the application of iPSC in MMD, aiming to provide an outlook of iPSC in molecular mechanism and novel therapies of MMD.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 6","pages":"733-739"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9919678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of Caspase-dependent Apoptosis in Rat Bone Marrow Mesenchymal Stem Cells Due to Di-2-Ethylhexyl Phthalate Toxicity was Found to Arrest the Cell Cycle at the G1 Stage.","authors":"Abnosi Mohammad Hussein,&nbsp;Sargolzaei Javad,&nbsp;Shayeganfar Zahra","doi":"10.2174/1574888X18666230106114727","DOIUrl":"https://doi.org/10.2174/1574888X18666230106114727","url":null,"abstract":"<p><strong>Background: </strong>Di-(2-ethylhexyl) phthalate (DEHP) is used as a plasticizer in polyvinyl chloride products which is widely utilized. Previously we found, DEHP reduced the viability and proliferation ability of bone marrow mesenchymal stem cells (BMSCs).</p><p><strong>Objective: </strong>In the present study, the mechanism of DEHP toxicity was investigated.</p><p><strong>Methods: </strong>Rat BMSCs were cultured up to 3^rd passage and their viability was determined after treatment with 100 and 500 μM of DEHP for 24 and 48 hours. The levels of sodium, potassium, and calcium as well as induction of apoptosis were investigated. Using flow cytometry, cell cycle analysis was performed and the expression of genes involved in the cell cycle was evaluated using reverse transcriptase-PCR. Data were analyzed and <i>p</i> < 0.05 was taken as the level of significance.</p><p><strong>Results: </strong>Although the viability and electrolyte level of BMSCs were not affected with 100 μM of DEHP, this environmental pollution induced caspase-dependent apoptosis in a concentration-dependent manner. In both of the concentrations, DEHP arrests the cell cycle at the G0/G1 phase, and the expression of Cdk2 and Cdk4 was significantly reduced whereas an over-expression of P53 was observed. However, the expression of the raf1 gene remained unchanged.</p><p><strong>Conclusion: </strong>DEHP induces caspase-dependent apoptosis in BMSCs and arrests the cell cycle due to the reduction of Cdk2 and Cdk4 expression via over-expression of P53.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 8","pages":"1106-1112"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9842108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}