Current stem cell research & therapy最新文献

{"title":"Amniotic Fluid Stem Cells: What They Are and What They Can Become.","authors":"Margit Rosner,&nbsp;Markus Hengstschläger","doi":"10.2174/1574888X16666211210143640","DOIUrl":"https://doi.org/10.2174/1574888X16666211210143640","url":null,"abstract":"<p><p>In the last two decades, fetal amniotic fluid stem cells progressively attracted attention in the context of both basic research and the development of innovative therapeutic concepts. They exhibit broadly multipotent plasticity with the ability to differentiate into cells of all three embryonic germ layers and low immunogenicity. They are convenient to maintain, highly proliferative, genomically stable, non-tumorigenic, perfectly amenable to genetic modifications, and do not raise ethical concerns. However, it is important to note that among the various fetal amniotic fluid cells, only c-Kit+ amniotic fluid stem cells represent a distinct entity showing the full spectrum of these features. Since amniotic fluid additionally contains numerous terminally differentiated cells and progenitor cells with more limited differentiation potentials, it is of highest relevance to always precisely describe the isolation procedure and characteristics of the used amniotic fluid-derived cell type. It is of obvious interest for scientists, clinicians, and patients alike to be able to rely on up-todate and concisely separated pictures of the utilities as well as the limitations of terminally differentiated amniotic fluid cells, amniotic fluid-derived progenitor cells, and c-Kit+ amniotic fluid stem cells, to drive these distinct cellular models towards as many individual clinical applications as possible.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 1","pages":"7-16"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10858223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of m6A in Osteoporosis and the Differentiation of Mesenchymal Stem Cells into Osteoblasts and Adipocytes.","authors":"Weifei Zhang,&nbsp;Tao Ke,&nbsp;Jianjing Lin,&nbsp;Peng Liu,&nbsp;Zhiping Guan,&nbsp;Jiapeng Deng,&nbsp;Deli Wang,&nbsp;Hui Zeng","doi":"10.2174/1574888X17666220621155341","DOIUrl":"https://doi.org/10.2174/1574888X17666220621155341","url":null,"abstract":"<p><p>Osteoporosis is a systemic disease in which bone mass decreases, leading to an increased risk of bone fragility and fracture. The occurrence of osteoporosis is believed to be related to the disruption of the differentiation of mesenchymal stem cells into osteoblasts and adipocytes. N6-adenylate methylation (m6A) modification is the most common type of chemical RNA modification and refers to a methylation modification formed by the nitrogen atom at position 6 of adenine (A), which is catalyzed by a methyltransferase. The main roles of m6A are the post-transcriptional level regulation of the stability, localization, transportation, splicing, and translation of RNA; these are key elements of various biological activities, including osteoporosis and the differentiation of mesenchymal stem cells into osteoblasts and adipocytes. The main focus of this review is the role of m6A in these two biological processes.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 3","pages":"339-346"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9677819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human Amniotic Fluid Stem Cells Exert Immunosuppressive Effects on T Lymphocytes in Allergic Rhinitis.","authors":"Ling Zong,&nbsp;De Wang,&nbsp;Yanbo Long,&nbsp;Xiaolan Liu,&nbsp;Ailin Tao,&nbsp;Lanzhen Zhang,&nbsp;Jinming Zhai","doi":"10.2174/1574888X17666220926105744","DOIUrl":"https://doi.org/10.2174/1574888X17666220926105744","url":null,"abstract":"<p><strong>Aim: </strong>The study aims to investigate the immunomodulatory effect of Amniotic fluid stem (AFS) cells to Th2-skewed allergic rhinitis (AR) on T-lymphocyte proliferation, viability, activation and cytokine production.</p><p><strong>Background: </strong>AFS cells can suppress peripheral blood mononuclear cells (PBMCs) proliferation and display immunomodulatory properties, but AFS cells' immunoregulation on AR has not been defined.</p><p><strong>Methods: </strong>Human AFS cells were derived from magnetic cell sorting and co-cultured with PBMCs from AR patients stimulated by phytohemagglutinin (PHA). The AFS cells-associated suppressive proliferation was analyzed using CellTrace™ Violet assay; the T lymphocytes proliferation, viability, activation and the Foxp3⁺ Treg cells were determined by flow cytometry; cytokine levels were measured using an enzyme- linked immunosorbent assay.</p><p><strong>Results: </strong>We determined that AFS cells significantly inhibited PHA-induced CD3⁺ T lymphocyte proliferation at the ratio higher than 1:50 (AFS cells: PBMCs) (<i>P</i><0.05); AFS cells obviously increased the T lymphocytes viability (<i>P</i><0.01), inhibited the apoptosis of T lymphocytes (<i>P</i><0.001), compared to PBMCs alone; AFS cells suppressed CD3⁺CD25⁺ T lymphocytes activated by PHA (<i>P</i><0.05); AFS cells significantly promote Treg cells expansion in house dust mite (HDM)-stimulated PBMCs from AR patients (<i>P</i><0.05). Compared with HDM-stimulated PBMCs, AFS cell co-culture predominantly decreased IL-4 level (<i>P</i><0.05), but increased IFN-γ and IL-10 levels (<i>P</i><0.01).</p><p><strong>Conclusion: </strong>AFS cells modulate the T-cells' immune imbalance towards Th2 suppression in AR, which can be used as a new cell banking for allergic airway diseases.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 8","pages":"1113-1119"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9840540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cancer Stem Cells and Anti-tumor Immunity.","authors":"Merve Yılmaz,&nbsp;Fuat Kaplan,&nbsp;Ilgen Mender,&nbsp;Sergei M Gryaznov,&nbsp;Z Gunnur Dikmen","doi":"10.2174/1574888X18666221017142032","DOIUrl":"https://doi.org/10.2174/1574888X18666221017142032","url":null,"abstract":"<p><p>Cancer stem cells (CSCs) are correlated with poor clinical outcomes due to their contribution to chemotherapy resistance and the formation of metastasis. Multiple cell surface and enzymatic markers have been characterized to identify CSCs, which is important for diagnosis, therapy, and prognosis. This review underlines the role of CSCs and circulating tumor cells (CTCs) in tumor relapse and metastasis, the characteristics of CSC and CTC biomarkers, and the techniques used to detect these cells. We also summarized novel therapeutic approaches toward targeting CSCs, especially focusing on the role of immune checkpoint blockades (ICB), such as anti-programmed death 1 (anti-PD1) and antiprogrammed death ligand-1 (anti-PDL1) therapies. Additionally, we address an intriguing new mechanism of action for small molecular drugs, such as telomere-targeted therapy 6-thio-2'deoxyguanosine (6- thio-dG), and how it reshapes tumor microenvironment to overcome ICB resistance. There are indications, that personalized cancer therapy targeting CSC populations in conjunction with immune-mediated strategy hold promise for the removal of residual therapy-resistant CSCs in the near future.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 4","pages":"445-459"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9863903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HOXA10 Expressing UCMSCs Transplantation Improved Endometrial Receptivity on Endometrial Injury.","authors":"Meixian Wu,&nbsp;Yuanyuan Li,&nbsp;Yiwei Wang,&nbsp;Yifan Li,&nbsp;Jinghui Li,&nbsp;Shuang Zhao,&nbsp;Lihua Sun,&nbsp;Jing Xie","doi":"10.2174/1574888X17666220919111814","DOIUrl":"https://doi.org/10.2174/1574888X17666220919111814","url":null,"abstract":"<p><strong>Background: </strong>Endometrial injury is considered the major cause of female infertility. Traditional therapies such as estrogen substitution therapy are not satisfactory due to individual variation in response to treatment, thereby warranting the use of alternative strategies such as stem cell therapy. Transplantation of stem cells, such as umbilical cord mesenchymal stem cells (UCMSCs), has been shown to improve endometrial healing. However, due to the effect of the intrauterine environment, the therapeutic effect of UCMSCs is limited, and its efficacy is unstable. HOXA10, encoded by the HOXA10 gene, plays an important role in endometrium morphology maintenance, proliferation, differentiation, and embryo implantation. Moreover, UCMSCs do not show HOXA10 expression.</p><p><strong>Objective: </strong>Our study aimed to evaluate the therapeutic effects of HOXA10-transfected UCMSCs on endometrial injury repair in vivo.</p><p><strong>Methods: </strong>First, we established T10-UCMSCs (UCMSCs transfected with HOXA10) for transplantation. To establish the endometrial injury model, we injected 95% ethanol into the uterine cavity and transplanted T10-UCMSCs into the uterine cavity from the cornua uteri. Fourteen days later, uteri were collected for histological and biochemical analysis of endometrial growth and receptivity.</p><p><strong>Results: </strong>Our results showed the endometrial receptivity was better in T10-UCMSCs group than in UCMSCs group, suggesting that HOXA10 could enhance the repairing ability of UCMSCs in the endometrium injury repair. More importantly, the fertility test showed that more embryos were implanted in the T10- UCMSCs group.</p><p><strong>Conclusion: </strong>Our results suggest that UCMSCs with HOXA10 expression could improve the therapeutic effects on endometrial injury repairing.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 7","pages":"1001-1012"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10278240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10021136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The SDF-1/CXCR4 Signaling Pathway Directs the Migration of Systemically Transplanted Bone Marrow Mesenchymal Stem Cells Towards the Lesion Site in a Rat Model of Spinal Cord Injury.","authors":"Andong Zhao,&nbsp;Manhon Chung,&nbsp;Yi Yang,&nbsp;Xiaohua Pan,&nbsp;Yu Pan,&nbsp;Sa Cai","doi":"10.2174/1574888X17666220510163245","DOIUrl":"https://doi.org/10.2174/1574888X17666220510163245","url":null,"abstract":"<p><strong>Background: </strong>It has been observed that bone marrow-derived mesenchymal stem cells (MSCs) migrate towards the injured spinal cord and promote functional recovery when systemically transplanted into the traumatized spinal cord. However, the mechanisms underlying their migration to the spinal cord remain poorly understood.</p><p><strong>Methods: </strong>In this study, we systemically transplanted GFP- and luciferase-expressing MSCs into rat models of spinal cord injury and examined the role of the stromal cell-derived factor 1 (SDF-1)/CXCR4 axis in regulating the migration of transplanted MSCs to the spinal cord. After intravenous injection, MSCs migrated to the injured spinal cord where the expression of SDF-1 was increased. Spinal cord recruitment of MSCs was blocked by pre-incubation with an inhibitor of CXCR4. Their presence correlated with morphological and functional recovery. In vitro, SDF-1 or cerebrospinal fluid (CSF) collected from SCI rats promoted a dose-dependent migration of MSCs in culture, which was blocked by an inhibitor of CXCR4 or SDF-1 antibody.</p><p><strong>Results and conclusion: </strong>The study suggests that SDF-1/CXCR4 interactions recruit exogenous MSCs to injured spinal cord tissues and may enhance neural regeneration. Modulation of the homing capacity may be instrumental in harnessing the therapeutic potential of MSCs.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 2","pages":"216-230"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9431081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of RNA Splicing in Regulation of Cancer Stem Cell.","authors":"Greesham Tripathi,&nbsp;Avantika Tripathi,&nbsp;Joel Johnson,&nbsp;Manoj Kumar Kashyap","doi":"10.2174/1574888X16666211207103628","DOIUrl":"https://doi.org/10.2174/1574888X16666211207103628","url":null,"abstract":"<jats:sec>\u0000<jats:title />\u0000<jats:p />\u0000</jats:sec>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 1","pages":"3-6"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9461790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adipose-derived Stem Cells: Potentials, Availability and Market Size in Regenerative Medicine.","authors":"Adele Soltani,&nbsp;Maryam Moradi,&nbsp;Aida Rezaei Nejad,&nbsp;Shabnam Moradi,&nbsp;Ehsan Javandoost,&nbsp;Hojjatollah Nazari,&nbsp;Arefeh Jafarian","doi":"10.2174/1574888X17666220413092750","DOIUrl":"https://doi.org/10.2174/1574888X17666220413092750","url":null,"abstract":"<p><p>Adipose-derived stem cells (ADSCs) have been described as one of the most potent and accessible human adult stem cells which can be utilized in various therapeutic approaches. Due to the wide variety of cytokines and GFs secreted by them, ADSCs can be used for controlled drug release. These cells can be used for proliferation and differentiation of tissues regardless of survival conditions and immunologic problems. Because of their ability to differentiate into various lineages, ADSCs can be used in musculoskeletal problems, diabetes, heart diseases, obesity, neurologic and nephrogenic diseases, and wound healing, as well as applications in regenerative medicine such as osteogenic, cartilage, tendon, muscle, skin, CNS, cardiac and vascularization, as well as liver and even periodontal regeneration. To maintain the highest viability and efficiency, companies that provide ADSCs should offer the best product quality to gain market share and scientists need to acquire an understanding of sources where they can find the best products available. Therefore, in this article, we have reviewed the available products, companies and the market size currently available for ADSCs. Enormous effort has been made to list the most important trials, products and companies currently existent in the field. To achieve better outcomes in scientific research, there is the need to compare the products available and choose the best option according to desired goals. Thus, this paper provides a valuable reference for those interested in the field of ADSCs and their applications.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 3","pages":"347-379"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9485458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem Cell and Oxidative Stress-Inflammation Cycle.","authors":"Hatice Dogan Buzoglu,&nbsp;Ayse Burus,&nbsp;Yasemin Bayazıt,&nbsp;Michel Goldberg","doi":"10.2174/1574888X17666221012151425","DOIUrl":"https://doi.org/10.2174/1574888X17666221012151425","url":null,"abstract":"<p><p>Under a variety of physical and experimental settings, stem cells are able to self-renew and differentiate into specialized adult cells. MSCs (mesenchymal stromal/stem cells) are multipotent stem cells present in a wide range of fetal, embryonic, and adult tissues. They are the progenitors of a variety of specialized cells and are considered crucial tools in tissue engineering. MSCs, derived from various tissues, including cord blood, placenta, bone marrow, and dental tissues, have been extensively examined in tissue repair, immune modulation, etc. Increasing the vitality of MSCs and restoring cellular mechanisms are important factors in treatment success. Oxidative stress harms cellular molecules such as DNA, proteins, and lipids due to the overproduction of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in cells and tissues or insufficiency of antioxidant systems that can inactivate them. Oxidative stress has a close link with inflammation as a pathophysiological process. ROS can mediate the expression of proinflammatory genes via intracellular signaling pathways and initiate the chronic inflammatory state. At the same time, inflammatory cells secrete a large number of reactive species that cause increased oxidative stress at sites of inflammation. In inflammatory diseases, the differentiation of stem cells and the regenerative and wound healing process can be affected differently by the increase of oxidative stress. Recent studies have indicated that dental pulp stem cells (DPSCs), as a resource of adult stem cells, are an attractive option for cell therapy in diseases such as neurological diseases, diabetes, cardiological diseases, etc., as well as its treatment potential in pulp inflammation. The future of oxidative stressinflammation cycle and/or ageing therapies involves the selective elimination of senescent cells, also known as senolysis, which prevents various age-related diseases. Most pathologies are implicated on the effects of ageing without exerting undesirable side effects.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 5","pages":"641-652"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9492195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the Focal Immunomodulatory Clues Influencing Mesenchymal Stem Cells in the Milieu of Intervertebral Disc Degeneration.","authors":"Maite Esquijarosa Hechavarria,&nbsp;Seidu A Richard","doi":"10.2174/1574888X17666220420134619","DOIUrl":"https://doi.org/10.2174/1574888X17666220420134619","url":null,"abstract":"<p><p>The intervertebral discs (IVDs) are a relatively mobile joint that interconnects vertebrae of the spine. Intervertebral disc degeneration (IVDD) is one of the leading causes of low back pain, which is most often related to patient morbidity as well as high medical costs. Patients with chronic IVDD often need surgery that may sometimes lead to biomechanical complications as well as augmented degeneration of the adjacent segments. Moreover, treatment modalities like rigid intervertebral fusion, dynamic instrumentation, as well as other surgical interventions are still controversial. Mesenchymal stem cells (MSCs) have exhibited to have immunomodulatory functions and the ability to differentiate into cartilage, making these cells possibly an epitome for IVD regeneration. Transplanted MSCs were able to repair IVDD back to the normal disc milieu via the activation of the generation of extracellular matrix (ECM) proteins such as aggrecan, proteoglycans and collagen types I and II. IVD milieu clues like, periostin, cluster of differentiation, tumor necrosis factor alpha, interleukins, chemokines, transforming growth factor beta, reactive oxygen species, toll-like receptors, tyrosine protein kinase receptor and disialoganglioside, exosomes are capable of influencing the MSCs during treatment of IVDD. ECM microenvironment clues above have potentials as biomarkers as well as accurate molecular targets for therapeutic intervention in IVDD.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":"18 1","pages":"62-75"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10860808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}