The SDF-1/CXCR4 Signaling Pathway Directs the Migration of Systemically Transplanted Bone Marrow Mesenchymal Stem Cells Towards the Lesion Site in a Rat Model of Spinal Cord Injury.

IF 2.1 4区医学 Q4 CELL & TISSUE ENGINEERING

Current stem cell research & therapy Pub Date : 2023-01-01 DOI:10.2174/1574888X17666220510163245

Andong Zhao, Manhon Chung, Yi Yang, Xiaohua Pan, Yu Pan, Sa Cai

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引用次数: 4

Abstract

Background: It has been observed that bone marrow-derived mesenchymal stem cells (MSCs) migrate towards the injured spinal cord and promote functional recovery when systemically transplanted into the traumatized spinal cord. However, the mechanisms underlying their migration to the spinal cord remain poorly understood.

Methods: In this study, we systemically transplanted GFP- and luciferase-expressing MSCs into rat models of spinal cord injury and examined the role of the stromal cell-derived factor 1 (SDF-1)/CXCR4 axis in regulating the migration of transplanted MSCs to the spinal cord. After intravenous injection, MSCs migrated to the injured spinal cord where the expression of SDF-1 was increased. Spinal cord recruitment of MSCs was blocked by pre-incubation with an inhibitor of CXCR4. Their presence correlated with morphological and functional recovery. In vitro, SDF-1 or cerebrospinal fluid (CSF) collected from SCI rats promoted a dose-dependent migration of MSCs in culture, which was blocked by an inhibitor of CXCR4 or SDF-1 antibody.

Results and conclusion: The study suggests that SDF-1/CXCR4 interactions recruit exogenous MSCs to injured spinal cord tissues and may enhance neural regeneration. Modulation of the homing capacity may be instrumental in harnessing the therapeutic potential of MSCs.

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在脊髓损伤大鼠模型中，SDF-1/CXCR4信号通路指导全身移植骨髓间充质干细胞向损伤部位的迁移

研究背景:骨髓间充质干细胞(MSCs)系统移植到损伤脊髓后，可向损伤脊髓迁移并促进功能恢复。然而，它们向脊髓迁移的机制仍然知之甚少。方法:在本研究中，我们将表达GFP和荧光素酶的MSCs系统移植到脊髓损伤大鼠模型中，并检测基质细胞衍生因子1 (SDF-1)/CXCR4轴在调节移植MSCs向脊髓迁移中的作用。经静脉注射后，MSCs迁移到损伤脊髓，SDF-1的表达增加。用CXCR4抑制剂预孵育可阻断脊髓间充质干细胞的募集。它们的存在与形态和功能恢复有关。体外，从SCI大鼠收集的SDF-1或脑脊液(CSF)促进培养中的MSCs的剂量依赖性迁移，该迁移被CXCR4抑制剂或SDF-1抗体阻断。结果和结论:研究提示SDF-1/CXCR4相互作用可将外源性MSCs招募到损伤脊髓组织，并可能促进神经再生。调节归巢能力可能有助于利用间充质干细胞的治疗潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current stem cell research & therapy CELL & TISSUE ENGINEERING-CELL BIOLOGY

CiteScore

4.20

自引率

3.70%

发文量

197

审稿时长

>12 weeks

期刊介绍： Current Stem Cell Research & Therapy publishes high quality frontier reviews, drug clinical trial studies and guest edited issues on all aspects of basic research on stem cells and their uses in clinical therapy. The journal is essential reading for all researchers and clinicians involved in stem cells research.