Current Pharmaceutical Analysis最新文献_第9页

Extraction and analysis of lipid raft proteins with detergent-and non detergent-based method 用洗涤剂和非洗涤剂法提取和分析脂筏蛋白

IF 0.6 4区医学

Current Pharmaceutical Analysis Pub Date : 2023-08-16 DOI: 10.2174/1573412919666230816090557

Yuchu Chen, Hongbei Liu, M. Adu-Frimpong, Chenlu Gu, Lu Zhao, Sheng Tian, Xiujun Li, Xia Cao, Shanshan Tong

{"title":"Extraction and analysis of lipid raft proteins with detergent-and non detergent-based method","authors":"Yuchu Chen, Hongbei Liu, M. Adu-Frimpong, Chenlu Gu, Lu Zhao, Sheng Tian, Xiujun Li, Xia Cao, Shanshan Tong","doi":"10.2174/1573412919666230816090557","DOIUrl":"https://doi.org/10.2174/1573412919666230816090557","url":null,"abstract":"\u0000\u0000Lipid raft is found on the cell membrane and is considered a microstructure\u0000rich in cholesterol, phospholipids and target proteins that are insoluble in nonionic detergents at low\u0000temperatures.rich in cholesterol, phospholipids and target proteins that are insoluble in nonionic detergents at low\u0000temperatures.\u0000\u0000\u0000\u0000In this study, detergent and non-detergent methods were used to extract lipid rafts from different cells. With β-cyclodextrin as the negative control group, we analyzed and compared the effects\u0000of different extraction methods on the composition of lipid rafts in Caco-2 and U251 cells using three\u0000kinds of lysate, namely detergent method 1, detergent method 2 and non-detergent method, which\u0000could be extracted and collected via sucrose density gradient centrifugation. Western blotting and\u0000immunofluorescence were utilized to determine the location of lipid rafts via the proteins Caveolin-1\u0000and Flotillin-1, which are the characteristic proteins P-gp and TrkA in cells. The total protein in the\u0000lipid raft was quantitatively determined through the BCA (detergent compatible) kit method.\u0000\u0000\u0000\u0000The results showed that the total amount of lipid raft proteins extracted via the detergent\u0000method was more than that of the non-detergent method, while the content of β-cyclodextrin control\u0000histone that caused disruption of lipid rafts structure was the lowest.\u0000\u0000\u0000\u0000The detergent method extracted more abundant lipid rafts than the non-detergent method.\u0000Detergent method 2 did not only extract more fat raft layers, but also the extracted highest total protein content, wherein it demonstrated better extraction effect with more lipid raft layers and higher expression of target protein P-gp.\u0000","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42130449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Metabolism Study of Oleraisoquinoline in Rats Using Ultrahigh-performance Liquid Chromatography-electrospray Coupled withQuadrupole Time-of-flight Mass Spectrometry and its Bioactivities 超高效液相色谱-电喷雾-四极杆飞行时间质谱联用研究奥莱异喹啉在大鼠体内的代谢及其生物活性

IF 0.6 4区医学

Current Pharmaceutical Analysis Pub Date : 2023-08-16 DOI: 10.2174/1573412919666230816090927

Rui-Fang Sun, Pei-Pei Liu, Jiayin Tian, Fan He, Xixiang Ying

{"title":"The Metabolism Study of Oleraisoquinoline in Rats Using Ultrahigh-performance Liquid Chromatography-electrospray Coupled with\u0000Quadrupole Time-of-flight Mass Spectrometry and its Bioactivities","authors":"Rui-Fang Sun, Pei-Pei Liu, Jiayin Tian, Fan He, Xixiang Ying","doi":"10.2174/1573412919666230816090927","DOIUrl":"https://doi.org/10.2174/1573412919666230816090927","url":null,"abstract":"\u0000\u0000This study aimed to investigate the main metabolites and metabolic pathways of oleraisoquinoline in rats, a new alkaloid isolated from Portulaca oleracea L., and test its\u0000antioxidation and anticholinesterase effects\u0000\u0000\u0000\u0000Ultra-high-performance liquid chromatography-electrospray coupled with quadrupole\u0000time-of-flight mass spectrometry (UHPLC-ESI-Q-TOF/MS) was applied to study the metabolism\u0000of oleraisoquinoline. Furthermore, 1,1‑diphenyl‑2‑picrylhydrazyl assay and modified Ellman’s\u0000method were used to test the antioxidation and anticholinesterase effects of oleraisoquinoline, respectively\u0000\u0000\u0000\u0000The metabolism results of oleraisoquinoline showed, after its administration through the\u0000tail vein of rats, 4 metabolites in the plasma samples, 17 metabolites in the urine sample, and 2\u0000metabolites in the feces sample. The main metabolic pathways were hydrolyzation, oxidation, hydroxylation, sulfonation, glucuronidation, acetylation, and methylation. Additionally, IC50 values\u0000of antioxidant and anticholinesterase activities were 13.819 ± 0.005 µM and 10.551 ± 0.069 µM,\u0000respectively.\u0000\u0000\u0000\u000021 metabolites were found in the rat’s plasma, urine, and feces samples, and the metabolic pathways included hydrolyzation, oxidation, hydroxylation, sulfonation, glucuronidation,\u0000acetylation, and methylation; among them, sulfonation was the main metabolic reaction. Meanwhile, oleraisoquinoline also showed extremely good antioxidant and anticholinesterase activities.\u0000","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42411552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boron-doped diamond electrochemical sensors: A promising approach in the therapy of human ailments 掺硼金刚石电化学传感器:治疗人类疾病的一种有前途的方法

IF 0.6 4区医学

Current Pharmaceutical Analysis Pub Date : 2023-08-09 DOI: 10.2174/1573412919666230809100748

Rohit Bhatia, Arshdeep Chopra, Yogindra Kumari, Santosh Kumar Verma

引用次数: 0

Advancements in the Analytical Methods for Ripasudil Hydrochloride Hydrate and Timolol Maleate: A Recently Approved FDC 盐酸Ripasudil水合物和马来酸Timolol分析方法的进展：一种最近批准的FDC

IF 0.6 4区医学

Current Pharmaceutical Analysis Pub Date : 2023-08-07 DOI: 10.2174/1573412919666230807114942

K. Patel, R. Kotadiya

{"title":"Advancements in the Analytical Methods for Ripasudil Hydrochloride Hydrate and Timolol Maleate: A Recently Approved FDC","authors":"K. Patel, R. Kotadiya","doi":"10.2174/1573412919666230807114942","DOIUrl":"https://doi.org/10.2174/1573412919666230807114942","url":null,"abstract":"\u0000\u0000Fixed-dose combinations (FDCs) combine two or more medications into a single dosage form. Several benefits, including impending therapeutic efficacy, a decline in episodes of adverse drug effects, pharmacokinetic advantages, a decrease in pill burden, a reduction in the dose of individual medications, and a reduction in the emergence of drug resistance, justify their acceptance. For the treatment of increased intraocular pressure (IOP) in adult patients with open-angle glaucoma or ocular hypertension, an FDC eye drop formulation, including ripasudil hydrochloride hydrate (0.4%W/V) and timolol maleate (0.5%W/V) has just received approval. No analytical method has been reported thus far for this newly approved combination. Thus, this review collected and simplified information on reported analytical techniques and physicochemical and biological properties for the above-cited FDCs. The authors have explored various authenticated scientific journals and presented simplified information to meet the objectives. In this study, the reported methods are spectroscopy (nil, 23%), HPTLC (nil, 10%), HPLC (100%, 61%), hyphenated techniques (nil, 6%) and electrophoresis methods (nil, 6%) for ripasudil hydrochloride hydrate and timolol maleate, respectively. Analysts using such comprehensive data might develop a method for analyzing the recently approved FDCs.\u0000","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46770221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification Of Methamphetamine and Amphetamine in Drug Abuse Urine Sample using Multi-drug Rapid Test and Gas Chromatography-Mass Spectrometry (GC-MS) at the Centre of Drug Testing Laboratory, National Narcotics Board Republic of Indonesia 使用多药快速试验和气相色谱-质谱联用(GC-MS)鉴定滥用药物尿液样本中的甲基苯丙胺和安非他命

IF 0.6 4区医学

Current Pharmaceutical Analysis Pub Date : 2023-08-03 DOI: 10.2174/1573412919666230803102859

Kurnia Sandy, T. Rahmania, Eri Arfiyani

{"title":"Identification Of Methamphetamine and Amphetamine in Drug Abuse Urine Sample using Multi-drug Rapid Test and Gas Chromatography-Mass Spectrometry (GC-MS) at the Centre of Drug Testing Laboratory, National Narcotics Board Republic of Indonesia","authors":"Kurnia Sandy, T. Rahmania, Eri Arfiyani","doi":"10.2174/1573412919666230803102859","DOIUrl":"https://doi.org/10.2174/1573412919666230803102859","url":null,"abstract":"\u0000\u0000Drug abuse is a real threat that threatens the Indonesian nation in the form of non-military threats. The second most abused illicit substance in the world is methamphetamine (MA). Most of the methamphetamine users in the world live in East and Southeast Asia. Methamphetamine is a powerful stimulant of the central nervous system (CNS) that can make someone feel an increase in sympathomimetic.\u0000\u0000\u0000\u0000First, the preliminary test was conducted using a BioCare multi-drug rapid test and a specific methamphetamine rapid test that works based on the Lateral Flow Assay (LFA) principle. Second, the sample preparation used Liquid-Liquid Extraction (LLE). Third, GC-MS confirmed the test using GC-MS Shimadzu-QP2020NX-GC-2030 (HP-5MS column, Length 30 m, diameter 0.25 m, film 0.25).\u0000\u0000\u0000\u0000The Preliminary test shows that the urine is positive and contains methamphetamine and amphetamine. Based on the sample chromatogram, There are 2 peaks, which are methamphetamine and amphetamine compounds it can be seen by the retention time is 5.560 minutes (methamphetamine) a similarity index of 98% to methamphetamine compounds, and 4.885 minutes (amphetamine) with a similarity index of 96% to amphetamine compounds.\u0000\u0000\u0000\u0000The urine samples with code 210DGB from methamphetamine users were detected due to the presence of methamphetamine and amphetamine, which is the metabolite of methamphetamine.\u0000","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48867919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TLC-surface enhanced Raman spectroscopy hyphenated technique: An excellent tool for modern analysis tlc -表面增强拉曼光谱联线技术:现代分析的一个优秀工具

IF 0.6 4区医学

Current Pharmaceutical Analysis Pub Date : 2023-07-26 DOI: 10.2174/1573412919666230726112245

P. Chawla, D. Das, Nikita Sharma

引用次数: 0

Researches on detection of related substances in drugs 药物中相关物质的检测研究

IF 0.6 4区医学

Current Pharmaceutical Analysis Pub Date : 2023-07-25 DOI: 10.2174/1573412919666230725143826

Yanjun Hao, Jianzhong Qi, Hongli Wang, Lu Dai, Lei Shi, Haigang Chen

{"title":"Researches on detection of related substances in drugs","authors":"Yanjun Hao, Jianzhong Qi, Hongli Wang, Lu Dai, Lei Shi, Haigang Chen","doi":"10.2174/1573412919666230725143826","DOIUrl":"https://doi.org/10.2174/1573412919666230725143826","url":null,"abstract":"\u0000\u0000The production of drugs is very complicated. In the process of preparation and transportation, mixing a small amount of reaction raw materials, intermediates, and storage degradation products is inevitable, which affect the quality of drugs. The quality of drugs not only affects\u0000the efficacy, but also produces unpredictable side effects and toxicity, which is directly related to\u0000people's life and health. The detection of drug quality has always been the focus of attention. Related substance research is one of the key projects in drug quality research, and its content is a direct indicator of drug purity. Therefore, it is very important to establish the content standard of\u0000drug related substances and develop the detection method of related substances. The related substances of drugs are rich in variety, with different physical and chemical properties, and the components of related substances, such as degradation products need further analysis, which makes it\u0000difficult to effectively apply the detection methods. After years of research, the detection methods\u0000of related substances are constantly updated and improved. At present, the detection methods of\u0000related substances involve physical, chemical, and other fields, including chemical methods,\u0000chromatography, and spectroscopy. In this paper, the research progress of detection methods for\u0000related substances in drugs in recent years was reviewed, and the types, features, and application\u0000scope of detection methods were summarized, providing reference for quality control of chemical\u0000synthetic drugs and traditional Chinese medicines. Therefore, this work provides new ideas for the\u0000development of more accurate and efficient detection methods.\u0000","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43251922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of Chemical Reagents in Non-Fluorescent Pharmaceutical Labels: A Comprehensive Review 化学试剂在非荧光药物标签中的应用综述

IF 0.6 4区医学

Current Pharmaceutical Analysis Pub Date : 2023-06-09 DOI: 10.2174/1573412919666230609120450

B. Devi, K. Madhavi, Swathi Naraparaju, Rajeshwari Deverakonda

引用次数: 0

Determination of Two Potential Genotoxic Impurities in Crisaborole API by UPLC-MS/MS hplc -MS/MS法测定Crisaborole原料药中两种潜在遗传毒性杂质

IF 0.6 4区医学

Current Pharmaceutical Analysis Pub Date : 2023-06-09 DOI: 10.2174/1573412919666230609152143

Q. Zou, Mengmeng Zhang

{"title":"Determination of Two Potential Genotoxic Impurities in Crisaborole API by UPLC-MS/MS","authors":"Q. Zou, Mengmeng Zhang","doi":"10.2174/1573412919666230609152143","DOIUrl":"https://doi.org/10.2174/1573412919666230609152143","url":null,"abstract":"\u0000\u0000Atopic dermatitis (AD) is an allergic skin disease that can be inherited. Crisaborole is a specific drug developed to deal with this disease. The compounds of 4-(4-Bromo-3-formyl-phenoxy)-benzonitrile and 4-(4-(4-bromo-3-formylphenoxy)-3- formylphenoxy)benzonitrile are the representative impurities in the synthesis of Crisaborole active pharmaceutical in gradient (API). Owing to the genotoxic impurities that could lead to cancer and gene mutations, it is necessary to develop a simple, efficient, sensitive, and accurate method to detect these impurities. In this study, the 4-(4-Bromo-3-formyl-phenoxy)-benzonitrile and 4-(4-(4-bromo-3-formylphenoxy)-3-formylphenoxy)benzo- nitrile present in Crisaborole API were detected using UPLC-MS/MS.\u0000\u0000\u0000\u0000The separation was made on a ZORBAX Eclipse XDB-Pheny column (4.6 mm ×75mm, 3.5 μm) with the mobile phase of acetonitrile solution containing 0.1% trifluoroacetic acid (A) and water containing 0.1% formic acid (B) in gradient elution mode. Quantification was performed using positive ion electrospray ionization (ESI), and the contents of two compounds were determined using the multiple reaction monitoring (MRM) modes.\u0000\u0000\u0000\u0000The quantitative analytical method was fully validated with respect to linearity (r>0.9998), sensitivity, precision, accuracy (the average recovery of two impurities was 84.1% to 90.7%), and robustness.\u0000\u0000\u0000\u0000Three batches of samples were detected by UPLC-MS/MS, indicating that the proposed approach was applicable for the quality evaluation of Crisaborole.\u0000","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42031469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Suitable HPLC Method for the Analysis of Impurities in Clorprenaline and Bromhexine Capsules and Identification of Unknown Impurities by 2D LC-Q-TOF MS 建立氯丙那林、溴甲基辛胶囊中杂质的高效液相色谱分析方法及二维LC-Q-TOF质谱法鉴定未知杂质

IF 0.6 4区医学

Current Pharmaceutical Analysis Pub Date : 2023-06-01 DOI: 10.2174/1573412919666230601140557

Yunfeng Shi, Liqin Lin, Qi Yao, Xiaojuan Ren, Fengmei Zhang

{"title":"Development of a Suitable HPLC Method for the Analysis of Impurities in Clorprenaline and Bromhexine Capsules and Identification of Unknown Impurities by 2D LC-Q-TOF MS","authors":"Yunfeng Shi, Liqin Lin, Qi Yao, Xiaojuan Ren, Fengmei Zhang","doi":"10.2174/1573412919666230601140557","DOIUrl":"https://doi.org/10.2174/1573412919666230601140557","url":null,"abstract":"\u0000\u0000In order to determine the impurities in compound clorprenaline and bromhexine capsules, a new stability-indicating HPLC method was established. A Boston Green ODS column was used, and the UV detection was 225nm. The influences of the gradient elution program, the composition ratio of the mobile phase and the velocity of flow on the separation of impurities in clorprenaline and bromhexine capsules were investigated. The developed methods were validated by assessing linearity, range, accuracy, the limit of detection (LOD), the limit of quantitation (LOQ), and robustness. The experimental results showed that the three components had good linearity, and the correlation coefficients were greater than 0.999. The recoveries of clorprenaline hydrochloride, decloxizine hydrochloride, and bromhexine hydrochloride were in the range of 95.0 to 102.3%. The LOD and LOQ values of clorprenaline hydrochloride, decloxizine hydrochloride, and bromhexine hydrochloride were between 0.20 and 0.82 μg•mL-1.\u0000Moreover, two unknown impurities were identified by two-dimensional liquid chromatography-tandem with quadrupole/time-of-flight mass spectrometry in electrospray ionization mode. Impurity 1 was derived from chlorohydroxazine hydrochloride, which has not been reported in the literature. Impurity 2 was derived from bromhexine hydrochloride, and its structure was the same as that of impurity E in EP 10.0 quality standard for bromhexine hydrochloride. The established method was highly specific, sensitive, accurate, and suitable for routine quality control of clorprenaline and bromhexine capsules. The structures of unknown impurities were characterized by the MS/MS data.\u0000","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42237321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0