Current Pharmaceutical Analysis最新文献

{"title":"Exploring the Mechanism of Dandelion in Treating Helicobacter pylori-Induced Chronic Gastritis Based on Network Pharmacology","authors":"Siying Qu, Puwan Huang, Xinyi Tian, Wenjie Lian, Xijun Tang","doi":"10.1016/j.cpan.2026.07.006","DOIUrl":"https://doi.org/10.1016/j.cpan.2026.07.006","url":null,"abstract":"Background Helicobacter pylori-induced chronic gastritis (HAG) is a significant healthcare burden in China due to its prevalence and carcinogenic potential. Current clinical management relies on antimicrobial therapies, such as bismuth-based quadruple therapy. This study aimed to explore Traditional Chinese Medicine (TCM)’s protective effect against long-term antibiotic therapy-induced injury, such as rising resistance, reduced eradication rates, and increasing adverse effects (gut microbiota dysbiosis and drug allergies). Methods To address these limitations, bioactive components of dandelion were screened from multiple TCM databases using ADME-based pharmacokinetic filters, and their targets were predicted using SwissTargetPrediction. Disease-associated targets were retrieved from GEO, GeneCards, and OMIM, followed by PPI network construction, GO/KEGG enrichment, molecular docking, and GSEA validation to prioritize candidate targets for experimental validation. Blood-absorbed components were further confirmed by UPLC–MS serum analysis. The therapeutic effects and underlying mechanisms were subsequently validated in an H. pylori-infected C57BL/6 mouse model and GES-1 cells through histological evaluation, qRT-PCR quantification of pathway-related gene expression, and Western blot analysis of PI3K/AKT total and phosphorylated protein levels. Results Quercetin was identified as the principal active component, demonstrating strong binding affinity to AKT1 and upregulating PI3K/AKT phosphorylation. In vivo, quercetin inhibited HAG progression and enhanced the efficacy of quadruple therapy, improving body weight recovery, inflammation resolution, and cure time. Conclusion Quercetin enhanced quadruple therapy by boosting immunity, shortening treatment duration, and promoting gastric mucosal recovery. While this study delineates the mechanism of a single active compound via the PI3K/AKT axis, the holistic therapeutic potential of dandelion may rely on multi-component synergistic interactions. Future large-scale clinical trials are needed to optimize TCM-antibiotic combinations, offering safer, personalized therapy for HAG.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148672147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study on the differential chemical composition of Radix Paeoniae Rubra and Radix Paeoniae Alba and their ameliorative effects in a rat model of cold coagulation, Qi stagnation, and blood stasis","authors":"Siwen Pan, Chunlei Yu, Haixue Kuang","doi":"10.1016/j.cpan.2026.04.003","DOIUrl":"https://doi.org/10.1016/j.cpan.2026.04.003","url":null,"abstract":"","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"22 4","pages":"301-310"},"PeriodicalIF":0.0,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating metabolomics, DPPH assay, and literature mining for quality marker prediction and the establishment of a quality control strategy for Ampelopsis grossedentata","authors":"Fan Xiao-Su, Yuan Zheng-Ying, Qin Yu-Shan, Lu Dong-Mei, Zhao Xin-Yu, Gao Si-Wei, Xue Qin, Zhu Rui-Yu, Wen Yong-qiang, Shen Fang-Ke, Hu Ping, Minghua Gu","doi":"10.1016/j.cpan.2025.12.008","DOIUrl":"https://doi.org/10.1016/j.cpan.2025.12.008","url":null,"abstract":"","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"22 1","pages":"74-85"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147917813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trifluridine/tipiracil exhibits potent antitumor activity in triple-negative breast cancer by targeting SKP2 and inducing cell cycle arrest and apoptosis","authors":"Erwei Hu, Y. WANG, Ge Chen, Chongyang Xu, Suhui Liu, Yufeng Zou, Xuan Luo, Yu Gong","doi":"10.1016/j.cpan.2025.12.005","DOIUrl":"https://doi.org/10.1016/j.cpan.2025.12.005","url":null,"abstract":"Trifluridine/Tipiracil (TFTD) is an innovative oral medication comprising trifluridine and tipiracil. While TFTD has shown efficacy in metastatic colorectal cancer, its therapeutic potential in triple-negative breast cancer (TNBC) remains unexplored. TNBC is an aggressive breast cancer subtype with limited treatment options and poor prognosis, making the development of effective therapies a significant clinical challenge. As chemotherapy remains the primary treatment strategy for TNBC, this study aims to investigate the efficacy of TFTD against TNBC and to elucidate its underlying mechanisms, which may provide a novel and promising therapeutic strategy for this difficult-to-treat disease. This study investigated the cytotoxic effects of TFTD on TNBC cells using CCK8 assays to determine IC50 values and Annexin V/PI staining to assess apoptosis and cell cycle progression. RNA-seq analysis was performed to identify treatment-related pathways, with findings validated through RT-qPCR and Western blotting. In vivo xenograft models were used to evaluate the therapeutic efficacy of TFTD. TFTD exhibited significant cytotoxic effects against TNBC cells, with IC50 values of 2.237 μmol (MDA-MB-231) and 2.792 μmol (BT-549). It induced apoptosis, reduced cells in the S-phase, and inhibited TNBC cell migration and invasion. RNA-seq analysis revealed significant downregulation of SKP2 and upregulation of GADD45A, E2F2, NF2, and CCND1. In vivo, TFTD significantly suppressed tumor growth and SKP2 expression. TFTD-mediated cytotoxicity and its ability to inhibit tumor growth in TNBC highlight its potential as a novel therapeutic candidate for addressing unmet clinical needs in TNBC treatment.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"22 1","pages":"35-43"},"PeriodicalIF":0.0,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147921538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferulic acid targets AKT1/STAT3/EGFR signaling to induce apoptosis and inhibit metastasis in gastric cancer cells","authors":"Lucy Li Liu, Chenhao Li, Yu Qi, Bingbo Zhang, Boyu Pan","doi":"10.1016/j.cpan.2025.11.002","DOIUrl":"https://doi.org/10.1016/j.cpan.2025.11.002","url":null,"abstract":"","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"22 1","pages":"44-48"},"PeriodicalIF":0.0,"publicationDate":"2025-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147381686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the potential anti-hepatocarcinoma mechanism of Ruangan Lidan Decoction through UHPLC-Q-TOF-MS/MS analysis and network pharmacology approaches","authors":"Liyin Huang, Tao Si, Ting Liang, Peishan Yu, Linglu Dun, Mingmin Zhang, Haihui Chen","doi":"10.1016/j.cpan.2025.10.004","DOIUrl":"https://doi.org/10.1016/j.cpan.2025.10.004","url":null,"abstract":"","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"22 1","pages":"49-61"},"PeriodicalIF":0.0,"publicationDate":"2025-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147887130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated analysis of liquid chromatography-mass spectrometry and network pharmacology identified active compounds of Paeoniae Radix Alba for myasthenia gravis treatment","authors":"Wenyu Li, Luya Jin, Liuyu Lin","doi":"10.1016/j.cpan.2025.08.008","DOIUrl":"https://doi.org/10.1016/j.cpan.2025.08.008","url":null,"abstract":"Paeoniae Radix Alba (PRA), also known as Baishao, has been used for the clinical treatment of a variety of diseases, however, its role in myasthenia gravis (MG) remains unexplored. Active compounds of PRA were screened by Liquid chromatography-mass spectrometry (LC-MS). Their targets were predicted and intersected with MG-related targets to develop a protein-protein interaction (PPI) network, followed by performing functional enrichment analysis to uncover key biological pathways involved. Meanwhile, differentially expressed microRNAs (DEmiRNAs) in MG were filtered to select the target genes, which were then overlapped with the common genes of PRA’s active components and MG to develop “ingredient-targets-pathway” and “ingredients-targets-miRNA” networks. Molecular docking was adopted to predict the binding between the active ingredients and the key genes. Our analysis identified four active components in PRA and their corresponding MG-related targets. Intersection analysis revealed that these targets were significantly enriched in key biological pathways, including neuroactive ligand-receptor interaction, neuroactive ligand signaling, HIF-1 signaling pathway, and response to xenobiotic stimulus. Protein-protein interaction (PPI) network analysis further uncovered 23 DEmiRNAs and 4989 common genes, which were predominantly associated with MAPK signaling pathway, endocytosis, proteasome-mediated ubiquitin-dependent catabolic process, histone modification. Finally, based on the networks of “ingredient-targets-pathway” and “ingredients-targets-miRNA”, a binding between SERPINE1 and Catechin was observed. This study applied LC-MS and network pharmacology to identify active components of PRA for the treatment of MG.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"21 8","pages":"384-392"},"PeriodicalIF":0.0,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147884241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of diagnostic biomarkers and therapeutic targets for abdominal aortic aneurysms based on disulfidptosis mechanism","authors":"Ronggui Chen, Junzhu Shi, Yipeng Zhang, Meirong Zhou","doi":"10.1016/j.cpan.2025.03.007","DOIUrl":"https://doi.org/10.1016/j.cpan.2025.03.007","url":null,"abstract":"Currently, there is a lack of hallmark diagnostic markers for abdominal aortic aneurysms (AAA). Recent evidence has suggested the efficacy of gene signature related to Disulfidptosis (a novel form of regulated cell death triggered by disulfide stress) for prognostic implication. Accordingly, our current study aims to identify some relevant markers for AAA not only for understanding its pathogenesis but also for improving treatment. Abnormal cell types in AAA were initially analyzed using single-cell RNA sequencing (scRNA-seq) to select differentially expressed genes (DEGs). Then, SLC7A11 -related genes were identified using RNA sequencing (RNA-seq) data and disulfidptosis genes. AAA-related key genes were screened based on their expression patterns. Protein-Protein Interaction Networks (PPI) were then employed to identify interacting genes and predict potential therapeutic agents for AAA. Finally, the role of LCP1 in AAA progression was validated through in vitro cellular assays. Compared to control samples, the number of macrophages/monocytes was the highest in AAA, suggesting that these cells were the key immune cells during AAA progression. Three overlapping genes ( LCP1 , MARCKS , and PTPRC ) were screened, and the expression patterns of LCP1 and PTPRC were consistent in multiple datasets. LCP1 and PTPRC showed an accurate diagnostic ability (AUC > 0.76) and were therefore considered as key genes in the disulfidptosis signature. Additionally, we observed strong and robust binding interactions between 1-METHYL-5-(2-PHENOXYMETHYL-PYRROLIDINE-1-SULFONYL)-1H-INDOLE-2,3-DIONE-PTPRC, as well as between Nandrolone- phenpropionate-LCP1 and PTPRC, indicating their potential as therapeutic targets for AAA. Cellular assays confirmed that inhibition of LCP1 suppressed the expressions of inflammatory factors in AAA. This research offered novel insights into the mechanisms of AAA progression based on disulfidptosis and discovered effective biomarkers for predicting the prognosis of AAA.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"21 4","pages":"225-237"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147919633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of Salvia miltiorrhiza compounds in hepatocellular carcinoma: a preliminary investigation based on computational analysis and liquid chromatography-tandem mass spectrometry","authors":"Guang‐Bo Ge, Xiaohui Jiang, Xiaofeng Tian, Yuan Zhou, Guangxin Cao","doi":"10.1016/j.cpan.2025.04.001","DOIUrl":"https://doi.org/10.1016/j.cpan.2025.04.001","url":null,"abstract":"The potential therapeutic effect of Salvia miltiorrhiza on hepatocellular carcinoma (HCC) has been expounded. This study commenced to identify the diagnostic biomarkers and predict the role of Salvia miltiorrhiza in HCC. TCGA-LIHC cohort and HCCDB18 dataset were acquired. Chemical components of Salvia miltiorrhiza were identified. Differentially expressed genes (DEGs) were identified, and prognosis-associated DEGs were screened. The compound-gene network was established, and the biomarkers were identified accordingly. A nomogram was constructed and the diagnostic performance was assessed. Immune infiltration was evaluated via “CIBERSORT” and “estimate” packages. Pathways involved in biomarkers were analyzed by GSEA_4.2.2 software. Molecular docking model was constructed applying AutoDocktools software. The cell viability, migration and invasion of HCC cell line treated with β-Estradiol with/out overexpressing AKR1C3 were assessed. A total of 57 compounds were identified from Salvia miltiorrhiza and 54 risk and 4 protective prognostic DEGs were found in TCGA-LIHC cohort and HCCDB18 dataset. Further, we established a compound-gene network of 10 Salvia miltiorrhiza components and 3 prognostic DEGs. Accordingly, AURKB , HDAC11 and AKR1C3 were identified as the biomarkers to construct a nomogram, which displayed a strong diagnostic performance in HCC. Three biomarkers were notably correlated with most immune cells and were mainly involved in the pathways related to tumor proliferation, cell cycle, apoptosis and energy supply. Molecular docking model indicated that 3 compounds from Salvia miltiorrhiza (Ethinylestradiol, β-Estradiol, and 16alpha-Hydroxyestrone) could stably bind to AKR1C3 . Thereinto, β-Estradiol can inhibit the proliferation, migration and invasion of HCC cells by targeting AKR1C3 . This study provided a solid foundation for further exploring the pathogenesis of HCC as well as novel diagnostic biomarkers treatment targets, and potential management drugs for HCC.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"21 4","pages":"249-264"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147904937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An acyl pyrrole compound 361B promotes angiogenesis and improves hindlimb ischemia recovery","authors":"You Li, Fang Lv, Feifei Yang, Fujun Dai, Yongrui Liu, Li Huang, Jinhua Wang, Yihui He, Zhengfang Yi, Mingyao Liu","doi":"10.1016/j.cpan.2025.03.004","DOIUrl":"https://doi.org/10.1016/j.cpan.2025.03.004","url":null,"abstract":"Diabetes is the most common type of chronic diseases word wide, accompanied by inadequate local angiogenesis and vascular lesions which is characterized by a gradual disorder function of the endothelial cells. Vascular complications which includes lower limb ischemia and chronic wound healing, are the main cause of death and disability in diabetic patients. Previous evidence has shown that restoring angiogenesis function of endothelial cells was detected as a key treatment project of diabetic complications. However, there is no specific medicine to treat the above diseases currently. Here, we make an effort to develop a new small molecule compound that promotes angiogenesis. To do this, we screen a library of small molecules and identify compound 361B, which significantly promote endothelial cell sprouting, tube formation, microvessels formation from aortic ring and neovascularization in mouse corneal. Moreover, 361B enhances the ability of angiogenesis in a diabetic mouse hindlimb ischemia model and a wound model. Meanwhile, 361B prevented HUVECs from starvation-induced apoptosis. Through Pull-down assays and SPR experiments, we have confirmed that 361B directly binds to vimentin in endothelial cells. Finally, it has been demonstrated through QPCR and Western Blotting Assay that 361B activates Akt/eNOS pathway to promote angiogenesis. As a conclusion, our data proves that 361B could be considered as a promising compound with the potential to treat diabetic vascular complications.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"21 3","pages":"192-202"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147916318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}