Cytometry Part B: Clinical Cytometry最新文献

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MAGIC-DR: An interpretable machine-learning guided approach for acute myeloid leukemia measurable residual disease analysis MAGIC-DR:一种用于急性髓性白血病可测量残留病分析的可解释机器学习指导方法。
IF 2.3 3区 医学
Cytometry Part B: Clinical Cytometry Pub Date : 2024-02-28 DOI: 10.1002/cyto.b.22168
Kevin Shopsowitz, Jack Lofroth, Geoffrey Chan, Jubin Kim, Makhan Rana, Ryan Brinkman, Andrew Weng, Nadia Medvedev, Xuehai Wang
{"title":"MAGIC-DR: An interpretable machine-learning guided approach for acute myeloid leukemia measurable residual disease analysis","authors":"Kevin Shopsowitz,&nbsp;Jack Lofroth,&nbsp;Geoffrey Chan,&nbsp;Jubin Kim,&nbsp;Makhan Rana,&nbsp;Ryan Brinkman,&nbsp;Andrew Weng,&nbsp;Nadia Medvedev,&nbsp;Xuehai Wang","doi":"10.1002/cyto.b.22168","DOIUrl":"10.1002/cyto.b.22168","url":null,"abstract":"<p>Multiparameter flow cytometry is widely used for acute myeloid leukemia minimal residual disease testing (AML MRD) but is time consuming and demands substantial expertise. Machine learning offers potential advancements in accuracy and efficiency, but has yet to be widely adopted for this application. To explore this, we trained single cell XGBoost classifiers from 98 diagnostic AML cell populations and 30 MRD negative samples. Performance was assessed by cross-validation. Predictions were integrated with UMAP as a heatmap parameter for an augmented/interactive AML MRD analysis framework, which was benchmarked against traditional MRD analysis for 25 test cases. The results showed that XGBoost achieved a median AUC of 0.97, effectively distinguishing diverse AML cell populations from normal cells. When integrated with UMAP, the classifiers highlighted MRD populations against the background of normal events. Our pipeline, MAGIC-DR, incorporated classifier predictions and UMAP into flow cytometry standard (FCS) files. This enabled a human-in-the-loop machine learning guided MRD workflow. Validation against conventional analysis for 25 MRD samples showed 100% concordance in myeloid blast detection, with MAGIC-DR also identifying several immature monocytic populations not readily found by conventional analysis. In conclusion, Integrating a supervised classifier with unsupervised dimension reduction offers a robust method for AML MRD analysis that can be seamlessly integrated into conventional workflows. Our approach can support and augment human analysis by highlighting abnormal populations that can be gated on for quantification and further assessment. This has the potential to speed up MRD analysis, and potentially improve detection sensitivity for certain AML immunophenotypes.</p>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"106 4","pages":"239-251"},"PeriodicalIF":2.3,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139982544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization of a flow cytometry test for routine monitoring of B cell maturation antigen targeted CAR in peripheral blood 优化用于常规监测外周血中 B 细胞成熟抗原靶向 CAR 的流式细胞仪检测。
IF 3.4 3区 医学
Cytometry Part B: Clinical Cytometry Pub Date : 2024-02-28 DOI: 10.1002/cyto.b.22165
Won-Ho Lee, Charlotte E. Graham, Hadley R. Wiggin, Hannah K. Nolan, Kiana J. Graham, Felix Korell, Mark B. Leick, Alexis L. Barselau, Estelle Emmanuel-Alejandro, Michael A. Trailor, Juliane M. Gildea, Frederic Preffer, Matthew J. Frigault, Marcela V. Maus, Kathleen M. E. Gallagher
{"title":"Optimization of a flow cytometry test for routine monitoring of B cell maturation antigen targeted CAR in peripheral blood","authors":"Won-Ho Lee,&nbsp;Charlotte E. Graham,&nbsp;Hadley R. Wiggin,&nbsp;Hannah K. Nolan,&nbsp;Kiana J. Graham,&nbsp;Felix Korell,&nbsp;Mark B. Leick,&nbsp;Alexis L. Barselau,&nbsp;Estelle Emmanuel-Alejandro,&nbsp;Michael A. Trailor,&nbsp;Juliane M. Gildea,&nbsp;Frederic Preffer,&nbsp;Matthew J. Frigault,&nbsp;Marcela V. Maus,&nbsp;Kathleen M. E. Gallagher","doi":"10.1002/cyto.b.22165","DOIUrl":"10.1002/cyto.b.22165","url":null,"abstract":"<p>Chimeric antigen receptor (CAR) modified T cell therapies targeting BCMA have displayed impressive activity in the treatment of multiple myeloma. There are currently two FDA licensed products, ciltacabtagene autoleucel and idecabtagene vicleucel, for treating relapsed and refractory disease. Although correlative analyses performed by product manufacturers have been reported in clinical trials, there are limited options for reliable BCMA CAR T detection assays for physicians and researchers looking to explore it as a biomarker for clinical outcome. Given the known association of CAR T cell expansion kinetics with toxicity and response, being able to quantify BCMA CAR T cells routinely and accurately in the blood of patients can serve as a valuable asset. Here, we optimized an accurate and sensitive flow cytometry test using a PE-conjugated soluble BCMA protein, with a lower limit of quantitation of 0.19% of CD3+ T cells, suitable for use as a routine assay for monitoring the frequency of BCMA CAR T cells in the blood of patients receiving either ciltacabtagene autoleucel or idecabtagene vicleucel.</p>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"106 3","pages":"162-170"},"PeriodicalIF":3.4,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139989514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recommendations for using artificial intelligence in clinical flow cytometry 在临床流式细胞仪中使用人工智能的建议。
IF 2.3 3区 医学
Cytometry Part B: Clinical Cytometry Pub Date : 2024-02-26 DOI: 10.1002/cyto.b.22166
David P. Ng, Paul D. Simonson, Attila Tarnok, Fabienne Lucas, Wolfgang Kern, Nina Rolf, Goce Bogdanoski, Cherie Green, Ryan R. Brinkman, Kamila Czechowska
{"title":"Recommendations for using artificial intelligence in clinical flow cytometry","authors":"David P. Ng,&nbsp;Paul D. Simonson,&nbsp;Attila Tarnok,&nbsp;Fabienne Lucas,&nbsp;Wolfgang Kern,&nbsp;Nina Rolf,&nbsp;Goce Bogdanoski,&nbsp;Cherie Green,&nbsp;Ryan R. Brinkman,&nbsp;Kamila Czechowska","doi":"10.1002/cyto.b.22166","DOIUrl":"10.1002/cyto.b.22166","url":null,"abstract":"<p>Flow cytometry is a key clinical tool in the diagnosis of many hematologic malignancies and traditionally requires close inspection of digital data by hematopathologists with expert domain knowledge. Advances in artificial intelligence (AI) are transferable to flow cytometry and have the potential to improve efficiency and prioritization of cases, reduce errors, and highlight fundamental, previously unrecognized associations with underlying biological processes. As a multidisciplinary group of stakeholders, we review a range of critical considerations for appropriately applying AI to clinical flow cytometry, including use case identification, low and high risk use cases, validation, revalidation, computational considerations, and the present regulatory frameworks surrounding AI in clinical medicine. In particular, we provide practical guidance for the development, implementation, and suggestions for potential regulation of AI-based methods in the clinical flow cytometry laboratory. We expect these recommendations to be a helpful initial framework of reference, which will also require additional updates as the field matures.</p>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"106 4","pages":"228-238"},"PeriodicalIF":2.3,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139971305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Translating the regulatory landscape of medical devices to create fit-for-purpose artificial intelligence (AI) cytometry solutions 转变医疗设备的监管环境,创建适用的人工智能(AI)细胞测量解决方案。
IF 2.3 3区 医学
Cytometry Part B: Clinical Cytometry Pub Date : 2024-02-23 DOI: 10.1002/cyto.b.22167
Goce Bogdanoski, Fabienne Lucas, Wolfgang Kern, Kamila Czechowska
{"title":"Translating the regulatory landscape of medical devices to create fit-for-purpose artificial intelligence (AI) cytometry solutions","authors":"Goce Bogdanoski,&nbsp;Fabienne Lucas,&nbsp;Wolfgang Kern,&nbsp;Kamila Czechowska","doi":"10.1002/cyto.b.22167","DOIUrl":"10.1002/cyto.b.22167","url":null,"abstract":"<p>The implementation of medical software and artificial intelligence (AI) algorithms into routine clinical cytometry diagnostic practice requires a thorough understanding of regulatory requirements and challenges throughout the cytometry software product lifecycle. To provide cytometry software developers, computational scientists, researchers, industry professionals, and diagnostic physicians/pathologists with an introduction to European Union (EU) and United States (US) regulatory frameworks. Informed by community feedback and needs assessment established during two international cytometry workshops, this article provides an overview of regulatory landscapes as they pertain to the application of AI, AI-enabled medical devices, and Software as a Medical Device in diagnostic flow cytometry. Evolving regulatory frameworks are discussed, and specific examples regarding cytometry instruments, analysis software and clinical flow cytometry in-vitro diagnostic assays are provided. An important consideration for cytometry software development is the modular approach. As such, modules can be segregated and treated as independent components based on the medical purpose and risk and become subjected to a range of context-dependent compliance and regulatory requirements throughout their life cycle. Knowledge of regulatory and compliance requirements enhances the communication and collaboration between developers, researchers, end-users and regulators. This connection is essential to translate scientific innovation into diagnostic practice and to continue to shape the development and revision of new policies, standards, and approaches.</p>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"106 4","pages":"294-307"},"PeriodicalIF":2.3,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139939780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Issue highlights—February 2024 本期重点--2024 年 2 月。
IF 3.4 3区 医学
Cytometry Part B: Clinical Cytometry Pub Date : 2024-02-22 DOI: 10.1002/cyto.b.22163
Virginia Litwin
{"title":"Issue highlights—February 2024","authors":"Virginia Litwin","doi":"10.1002/cyto.b.22163","DOIUrl":"10.1002/cyto.b.22163","url":null,"abstract":"&lt;p&gt;It is a pleasure to usher in the first issue of &lt;i&gt;Cytometry Part B: Clinical Cytometry&lt;/i&gt; for the New Year. I would like to take this opportunity to wish the International Society for Clinical Cytometry, the European Society for Clinical Cell Analyses, and Cytometry Part B, continued success in 2024. Also, I would like to thank all the people who make each issue of our journal possible, the submitting authors, the reviewers, the Editorial Board, the Associate Editors, Deputy Editor, Janos Kappelmayer, and our Editor-in-Chief, Fred Preffer. And last, but certainly not least, special thanks to our Managing Editor, Doris Regal who somehow makes it all come together, each and every issue.&lt;/p&gt;&lt;p&gt;In this issue, the importance of multiparametric flow cytometry in clinical diagnosis and drug development is highlighted with many of the papers echoing my passion for standardization, validation, and quality control.&lt;/p&gt;&lt;p&gt;The paper from the laboratories of Wang et al. (&lt;span&gt;2024&lt;/span&gt;), “Standardization of Flow Cytometric Detection of Antigen Expression,” is the result of a collaboration between the National Institute of Standards and Technology (NIST) and the National Cancer Institute (NCI) and promises to be one of the most important papers of the year (Tian et al., &lt;span&gt;2024&lt;/span&gt;). This point is highlighted by the Commentary on the paper by Bruce Davis, “Editorial on IVD cellular assay validation” (Davis, &lt;span&gt;2024&lt;/span&gt;). Both documents are ones that everyone conducting cytometry, in any setting, needs to read and re-read. They bring us one step closer to understanding what is required in order to achieve reproducible and quantitative flow cytometry data across platforms and across laboratories.&lt;/p&gt;&lt;p&gt;These manuscripts highlight the increased importance of accurately measuring antigen expression levels when treating patients with novel immunotherapies. Antigen density measurements not only impact patient selection, but are also instrumental in determining treatment efficacy and patient outcomes. The Tian et al. paper ultimately concludes that assay standardization is a critical requirement to enable broad clinical utility and impact of this novel class of therapies. A good part of the paper focuses on the inherent variability and subjectivity in qualitative estimates of antigen density (e.g., dim, moderate, bright) and the resulting need for quantitative measurements of cell surface antigen expression. Common methods for determining antigen density such as geometric mean fluorescence intensity (GeoMFI) and antibodies bound per cell (ABC) appear to be straightforward; however, result comparability across different instrument platforms, reagent lots, operators, and laboratories has not yet been demonstrated. Using a systematic, well-thought-out approach, this team evaluated assay variability of flow cytometric quantitation and then describe procedures and quality control practices whereby highly reproduceable antigen expression measurements ca","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"106 1","pages":"7-8"},"PeriodicalIF":3.4,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cyto.b.22163","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139930455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Flow cytometry of DNMT1 as a biomarker of hypomethylating therapies 将 DNMT1 流式细胞术作为低甲基化疗法的生物标记。
IF 3.4 3区 医学
Cytometry Part B: Clinical Cytometry Pub Date : 2024-02-12 DOI: 10.1002/cyto.b.22158
Philip G. Woost, Basem M. William, Brenda W. Cooper, Masumi Ueda Oshima, Folashade Otegbeye, Marcos J. De Lima, David Wald, Reda Z. Mahfouz, Yogen Saunthararajah, Tammy Stefan, James W. Jacobberger
{"title":"Flow cytometry of DNMT1 as a biomarker of hypomethylating therapies","authors":"Philip G. Woost,&nbsp;Basem M. William,&nbsp;Brenda W. Cooper,&nbsp;Masumi Ueda Oshima,&nbsp;Folashade Otegbeye,&nbsp;Marcos J. De Lima,&nbsp;David Wald,&nbsp;Reda Z. Mahfouz,&nbsp;Yogen Saunthararajah,&nbsp;Tammy Stefan,&nbsp;James W. Jacobberger","doi":"10.1002/cyto.b.22158","DOIUrl":"10.1002/cyto.b.22158","url":null,"abstract":"<p>The 5-azacytidine (AZA) and decitabine (DEC) are noncytotoxic, differentiation-inducing therapies approved for treatment of myelodysplastic syndrome, acute myeloid leukemias (AML), and under evaluation as maintenance therapy for AML postallogeneic hematopoietic stem cell transplant and to treat hemoglobinapathies. Malignant cell cytoreduction is thought to occur by S-phase specific depletion of the key epigenetic regulator, DNA methyltransferase 1 (DNMT1) that, in the case of cancers, thereby releases terminal-differentiation programs. DNMT1-targeting can also elevate expression of immune function genes (HLA-DR, MICA, MICB) to stimulate graft versus leukemia effects. In vivo, there is a large inter-individual variability in DEC and 5-AZA activity because of pharmacogenetic factors, and an assay to quantify the molecular pharmacodynamic effect of DNMT1-depletion is a logical step toward individualized or personalized therapy. We developed and analytically validated a flow cytometric assay for DNMT1 epitope levels in blood and bone marrow cell subpopulations defined by immunophenotype and cell cycle state. Wild type (WT) and DNMT1 knock out (DKO) HC116 cells were used to select and optimize a highly specific DNMT1 monoclonal antibody. Methodologic validation of the assay consisted of cytometry and matching immunoblots of HC116-WT and -DKO cells and peripheral blood mononuclear cells; flow cytometry of H116-WT treated with DEC, and patient samples before and after treatment with 5-AZA. Analysis of patient samples demonstrated assay reproducibility, variation in patient DNMT1 levels prior to treatment, and DNMT1 depletion posttherapy. A flow-cytometry assay has been developed that in the research setting of clinical trials can inform studies of DEC or 5-AZA treatment to achieve targeted molecular pharmacodynamic effects and better understand treatment-resistance/failure.</p>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"106 1","pages":"11-24"},"PeriodicalIF":3.4,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cyto.b.22158","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhancing HLA-B27 antigen detection: Leveraging machine learning algorithms for flow cytometric analysis. 加强 HLA-B27 抗原检测:利用机器学习算法进行流式细胞分析。
IF 3.4 3区 医学
Cytometry Part B: Clinical Cytometry Pub Date : 2024-02-12 DOI: 10.1002/cyto.b.22164
Sándor Baráth, Parvind Singh, Zsuzsanna Hevessy, Anikó Ujfalusi, Zoltán Mezei, Mária Balogh, Marianna Száraz Széles, János Kappelmayer
{"title":"Enhancing HLA-B27 antigen detection: Leveraging machine learning algorithms for flow cytometric analysis.","authors":"Sándor Baráth, Parvind Singh, Zsuzsanna Hevessy, Anikó Ujfalusi, Zoltán Mezei, Mária Balogh, Marianna Száraz Széles, János Kappelmayer","doi":"10.1002/cyto.b.22164","DOIUrl":"https://doi.org/10.1002/cyto.b.22164","url":null,"abstract":"<p><p>As the association of human leukocyte antigen B27 (HLA-B27) with spondylarthropathies is widely known, HLA-B27 antigen expression is frequently identified using flow cytometric or other techniques. Because of the possibility of cross-reaction with off target antigens, such as HLA-B7, each flow cytometric technique applies a \"gray zone\" reserved for equivocal findings. Our aim was to use machine learning (ML) methods to classify such equivocal data as positive or negative. Equivocal samples (n = 99) were selected from samples submitted to our institution for clinical evaluation by HLA-B27 antigen testing. Samples were analyzed by flow cytometry and polymerase chain reaction. Features of histograms generated by flow cytometry were used to train and validate ML methods for classification as logistic regression (LR), decision tree (DT), random forest (RF) and light gradient boost method (GBM). All evaluated ML algorithms performed well, with high accuracy, sensitivity, specificity, as well as negative and positive predictive values. Although, gradient boost approaches are proposed as high performance methods; nevertheless, their effectiveness may be lower for smaller sample sizes. On our relatively smaller sample set, the random forest algorithm performed best (AUC: 0.92), but there was no statistically significant difference between the ML algorithms used. AUC values for light GBM, DT, and LR were 0.88, 0.89, 0.89, respectively. Implementing these algorithms into the process of HLA-B27 testing can reduce the number of uncertain, false negative or false positive cases, especially in laboratories where no genetic testing is available.</p>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of flow cytometric immunophenotyping in the diagnosis of breast implant-associated anaplastic large cell lymphoma: A 6-year, single-institution experience 流式细胞免疫分型在诊断乳腺植入相关性无性大细胞淋巴瘤中的作用:6年单一机构经验。
IF 3.4 3区 医学
Cytometry Part B: Clinical Cytometry Pub Date : 2024-01-31 DOI: 10.1002/cyto.b.22162
Alexander Chan, Romany Auclair, Qi Gao, Paola Ghione, Steven Horwitz, Ahmet Dogan, Mikhail Roshal, Oscar Lin
{"title":"Role of flow cytometric immunophenotyping in the diagnosis of breast implant-associated anaplastic large cell lymphoma: A 6-year, single-institution experience","authors":"Alexander Chan,&nbsp;Romany Auclair,&nbsp;Qi Gao,&nbsp;Paola Ghione,&nbsp;Steven Horwitz,&nbsp;Ahmet Dogan,&nbsp;Mikhail Roshal,&nbsp;Oscar Lin","doi":"10.1002/cyto.b.22162","DOIUrl":"10.1002/cyto.b.22162","url":null,"abstract":"<p>Breast implant-associated anaplastic large cell lymphoma (BIA-ALCL) is an uncommon mature T-cell neoplasm occurring in patients with textured breast implants, typically after 7–10 years of exposure. Although cytopathologic or histopathologic assessment is considered the gold standard diagnostic method for BIA-ALCL, flow cytometry (FC)-based immunophenotyping is recommended as an adjunct test. However, the diagnostic efficacy of FC is not well reported. We reviewed 290 FC tests from breast implant pericapsular fluid and capsule tissue from 182 patients, including 16 patients with BIA-ALCL over a 6-year period, calculating diagnostic rates and test efficacy. FC showed an overall sensitivity of 75.9%, specificity of 100%, and negative and positive predictive values of 95.4% and 100%, respectively. Blinded expert review of false-negative cases identified diagnostic pitfalls, improving sensitivity to 96.6%. Fluid samples had better rates of adequate samples for FC testing compared with tissue samples. Paired with FC testing of operating room (OR)-acquired fluid samples, capsulectomy FC specimens added no diagnostic value in patients with concurrent fluid samples; no cases had positive capsule FC with negative fluid FC. Fluid samples are adequate for FC testing more often than tissue. Capsule tissue FC specimens do not improve FC efficacy when paired with OR-acquired fluid FC samples and are often inadequate samples. FC is 100% specific for BIA-ALCL and can serve as a confirmatory test but should not be the sole diagnostic method. Awareness of sample-specific diagnostic pitfalls greatly improves the sensitivity of BIA-ALCL testing by FC.</p>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"106 2","pages":"117-125"},"PeriodicalIF":3.4,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139650467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ROR1 expression in mature B lymphoid neoplasms by flow cytometry 流式细胞仪检测成熟 B 淋巴肿瘤中 ROR1 的表达。
IF 3.4 3区 医学
Cytometry Part B: Clinical Cytometry Pub Date : 2024-01-25 DOI: 10.1002/cyto.b.22157
Flávia Arandas de Sousa, Nádila Magalhães Millan, Rodolfo Patussi Correia, Andressa da Costa Vaz, Daniela Schimidell, Priscila Carmona Miyamoto, Marilia Sandoval Passaro, Bruna Garcia Nogueira, Elizabeth Xisto Souto, Nydia Strachman Bacal, Laiz Camerão Bento
{"title":"ROR1 expression in mature B lymphoid neoplasms by flow cytometry","authors":"Flávia Arandas de Sousa,&nbsp;Nádila Magalhães Millan,&nbsp;Rodolfo Patussi Correia,&nbsp;Andressa da Costa Vaz,&nbsp;Daniela Schimidell,&nbsp;Priscila Carmona Miyamoto,&nbsp;Marilia Sandoval Passaro,&nbsp;Bruna Garcia Nogueira,&nbsp;Elizabeth Xisto Souto,&nbsp;Nydia Strachman Bacal,&nbsp;Laiz Camerão Bento","doi":"10.1002/cyto.b.22157","DOIUrl":"10.1002/cyto.b.22157","url":null,"abstract":"<p>Immunophenotyping by flow cytometry is an integral part of the diagnosis and classification of leukemias/lymphomas. The expression of ROR1 associated with chronic B lymphocytic leukemia (CLL) is well described in the literature, both in its diagnosis and in the follow-up of minimal residual disease (MRD) research, however, there are few studies regarding the expression pattern of ROR1 in other subtypes of mature B lymphoid neoplasms. With the aim of evaluating the expression of ROR1 and associating it with the expression of other important markers for the differentiation of mature B lymphoid neoplasms (MBLN), 767 samples of cases that entered our laboratory for immunophenotyping with clinical suspicion of MBLN were studied. ROR1 expression is predominant in CD5+/CD10− neoplasms. Overall, positive ROR1 expression was observed in 461 (60.1%) cases. The CD5+/CD10− group had a significantly higher proportion of ROR1 positive samples (89.9%) and more brightly expressed ROR1 than the other groups. Our results highlight the importance of evaluating ROR1 expression in the diagnosis of MBLN to contribute to the differential diagnosis, and possibly therapy of mainly CLL, and indicate that this marker could be considered as a useful addition to immunophenotypic panels, particularly for more challenging cases.</p>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"106 1","pages":"74-81"},"PeriodicalIF":3.4,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139564045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Maturational dyssynchrony in benign B-cell precursors following lymphocyte depleting chemotherapy: A potential pitfall for B-lymphoblastic leukemia minimal/measurable residual disease (MRD) flow cytometry analysis 淋巴细胞耗竭化疗后良性 B 细胞前体的成熟不同步:B淋巴细胞白血病最小/可测量残留病(MRD)流式细胞术分析的潜在陷阱。
IF 3.4 3区 医学
Cytometry Part B: Clinical Cytometry Pub Date : 2024-01-21 DOI: 10.1002/cyto.b.22161
Alexander Placek, Brian Lockhart, Karin P. Miller, Gerald B. Wertheim, Shannon L. Maude, Brent L. Wood, Alexandra E. Kovach
{"title":"Maturational dyssynchrony in benign B-cell precursors following lymphocyte depleting chemotherapy: A potential pitfall for B-lymphoblastic leukemia minimal/measurable residual disease (MRD) flow cytometry analysis","authors":"Alexander Placek,&nbsp;Brian Lockhart,&nbsp;Karin P. Miller,&nbsp;Gerald B. Wertheim,&nbsp;Shannon L. Maude,&nbsp;Brent L. Wood,&nbsp;Alexandra E. Kovach","doi":"10.1002/cyto.b.22161","DOIUrl":"10.1002/cyto.b.22161","url":null,"abstract":"","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"106 2","pages":"138-141"},"PeriodicalIF":3.4,"publicationDate":"2024-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139511522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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