Cytometry Part B: Clinical Cytometry最新文献

{"title":"An unusual pattern observed upon the addition of CD79b to a flow cytometry B-cell lymphoma panel.","authors":"Vandana Panakkal, Raniah Al Amri, Stacey Mamatas, Wendy Shallenberger, Sara A Monaghan, Ahmad Al-Attar","doi":"10.1002/cyto.b.22246","DOIUrl":"https://doi.org/10.1002/cyto.b.22246","url":null,"abstract":"","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144625560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing detection of central nervous system involvement in multiple myeloma: A novel multidimensional dot-plot based analysis for flow cytometry.","authors":"Bettina Palicskó, Luca Janovák, László Rejtő, László Váróczy, Zsuzsanna Hevessy, Bettina Kárai","doi":"10.1002/cyto.b.22245","DOIUrl":"https://doi.org/10.1002/cyto.b.22245","url":null,"abstract":"<p><p>Central nervous system (CNS) involvement in multiple myeloma (MM) is a rare but severe complication with a poor prognosis. The identification of malignant plasma cells in cerebrospinal fluid (CSF) is essential for early diagnosis and intervention. However, the sensitivity of traditional diagnostic methods like cytology is low, especially in samples with low-cell counts. This study aimed to develop a multidimensional radar dot-plot analysis using Kaluza software to enhance the sensitivity and specificity of flow cytometry for detecting abnormal plasma cells in CSF. One hundred and twenty-five CSF samples were sent for flow cytometric testing to investigate the central nervous system involvement of MM. Finally, 89 samples from 40 patients were included in our study. Multicolor flow cytometry was performed using an 8-color labeling method, and radar dot-plot analysis was developed using diagnostic bone marrow samples to distinguish normal plasma cells, abnormal plasma cells, and cellular debris. The sensitivity of the novel method was evaluated by diluting myeloma bone marrow cells in pooled CSF samples to simulate low cell counts. Of the 125 CSF specimens, 16 samples from 4 patients showed abnormal plasma cells using both conventional and multidimensional flow cytometry analysis. Discordant results were found in 32 samples (25%), where conventional analysis suggested the presence of abnormal cells, but these were ruled out by multidimensional analysis. Sensitivity testing showed that the multidimensional dot-plot method outperforms conventional two-dimensional dot-plot analysis, as the radar dot plot can be used to identify abnormal cells in samples diluted to 5 WBC/μL, where the cell count of abnormal plasma cells is < 1 cell/μL. Our results showed that the new radar dot-plot analysis can increase the sensitivity and specificity of flow cytometry in MM for the detection of CNS involvement, even in low-cell-count CSF samples, regardless of whether the sample was obtained in a tube containing special reagent or not (TransFix/EDTA CSF Sample Storage tubes). This approach improves diagnostic accuracy, reduces the number of false positive cases caused by antibodies adhering to cell debris, and provides a reliable tool for assessing neurological complications in MM. Further validation is needed in a larger number of cases and testing of the method on different antibody panels.</p>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An uncommon case of acute megakaryoblastic leukemia with DDX3X::MLLT10 fusion gene.","authors":"Ting Li, Ping Wu, Man Chen, Xiuyun Zhao, Aixian Wang, Hui Wang","doi":"10.1002/cyto.b.22243","DOIUrl":"https://doi.org/10.1002/cyto.b.22243","url":null,"abstract":"","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A 20-color 21-antigen flow cytometric assay for disease monitoring of T-cell lymphoblastic leukemia.","authors":"Qi Gao, Jingping Zhang, Krasimira Rozenova, Xiaotian Sun, Amanda Burke, Olivia Miu, Nghia Nguyen, Shu Jie Zhang, Mikhail Roshal","doi":"10.1002/cyto.b.22242","DOIUrl":"https://doi.org/10.1002/cyto.b.22242","url":null,"abstract":"<p><p>T-lineage acute lymphoblastic leukemia (T-ALL) is an aggressive neoplasm of immature T cells. Flow cytometry plays a critical role in the diagnosis and management of the disease. It is used to establish the abnormal immature T-cell phenotype and to distinguish the early T-cell precursor (ETP)-ALL from more mature types at diagnosis. The evaluation of mediastinal disease is often complicated by the difficulty of the phenotypic distinction between the normal thymic precursors and the abnormal T lymphoblasts. Follow-up measurements of minimal/measurable residual disease (MRD) are critical for therapy decision-making and prognostication. In the MRD setting, flow cytometry requires a high degree of analytical expertise and assessment of numerous antigens. To address the diagnostic and monitoring challenges, we developed a single-tube 21-antigen assessment with simplified analysis. The assay distinguishes between normal thymic precursors and T lymphoblasts in tissue samples, enables evaluation of ETP versus non-ETP phenotypes, and allows for MRD assessment below 0.01% robust to antigenic changes.</p>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144552552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frequency and stability of populations of CD4⁺ and CD4⁺CD25⁺Foxp3⁺CD127^lo Treg in healthy adults defined by cytometry using monoclonal antibodies to T cell associated molecules.","authors":"Nirupama D Verma, Ranje Al-Atiyah, Prateek Rakesh, Andrew D Lam, Christopher Chiu, Giang T Tran, Bruce M Hall, Suzanne J Hodgkinson","doi":"10.1002/cyto.b.22241","DOIUrl":"https://doi.org/10.1002/cyto.b.22241","url":null,"abstract":"<p><p>Monitoring subpopulations of CD4⁺ T cells in blood, especially regulatory CD4⁺CD25⁺Foxp3⁺CD127^loT cells, has the potential to identify tolerance to transplants and defects that cause autoimmunity. CD45RA is expressed by naïve/resting CD4⁺, not by activated cells. Staining CD45RA with CD25 or Foxp3 identifies five populations of CD4⁺ T cells, three Treg, and two CD4⁺Foxp3^-T cells. CD25 is induced on activation of effector cells and is constitutively expressed by Treg. Examining Foxp3⁺ cells in CD4⁺CD25⁺CD127^lo, identified three Treg populations. It is not known how stable these populations of CD4⁺T cells are within individuals and between individuals. Repeated estimations over 3 years in 10 HV showed the proportion of cells in the three Treg populations was stable, whereas the two Foxp3^- populations varied. In a larger cohort of 110 samples, females had higher numbers of CD4⁺ cells than males. As a percentage of lymphocytes, there was no sex difference in the proportion of cells in the five populations. With age, there were fewer total Treg, with fewer resting Treg but an increase in activated Treg. Activation of both naïve CD4⁺ T cells and Treg induces expression of chemokine receptors associated with Th1, Th17, and Th2 responses that promote their infiltration into sites of inflammation. Activated Treg expressed CCR4 and, in addition, expressed CXCR3 (Th1), CCR6 (Th17), or neither CXCR3 nor CCR6 (Th2). Some Treg expressed both CCR6 and CXCR3. HLA-DR and CD39 were expressed by activated Treg, and many cells expressed both. There was low PD-1 expression. The stability of the major Treg populations suggested it could be feasible to establish normal ranges for the three Treg populations. Staining for chemokine receptors and Treg effector molecules in activated Treg populations may detect changes in immune homeostasis and tolerance.</p>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue highlights—June 2025","authors":"Joseph A. DiGiuseppe","doi":"10.1002/cyto.b.22239","DOIUrl":"https://doi.org/10.1002/cyto.b.22239","url":null,"abstract":"","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"108 3","pages":"195-197"},"PeriodicalIF":2.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remembering Dr. Bruce H. Davis: A passionate leader in clinical flow cytometry","authors":"","doi":"10.1002/cyto.b.22240","DOIUrl":"https://doi.org/10.1002/cyto.b.22240","url":null,"abstract":"","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"108 3","pages":"189-194"},"PeriodicalIF":2.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraoperative flow cytometry in detecting free carcinoma cells in peritoneal lavage fluid of gastric carcinoma cases.","authors":"Thulasi Raman Ramalingam, Bharaneedharan Marimuthu, Harsha N Rasheed, Archana Lakshmanan, Swetha Lakshmi Narla, Lakshman Vaidhyanathan, Ajit Pai","doi":"10.1002/cyto.b.22238","DOIUrl":"https://doi.org/10.1002/cyto.b.22238","url":null,"abstract":"<p><p>The free carcinoma cells (FCC) in peritoneal lavage fluid are an independent adverse prognostic factor in patients with gastric carcinoma. Detection of FCC in the pre-operative peritoneal lavage fluid is critical, as patients with FCC do not have a survival advantage with curative cytoreductive (CCR) surgery. Cytology is currently used to assess FCC, but its sensitivity is poor and there is a need for better sensitive techniques. We attempted to study the efficiency of intra-operative flow cytometry (FCM) in detecting FCC in peritoneal lavage fluid of gastric carcinoma patients. In this prospective study, 32 peritoneal lavage fluids were analyzed intra-operatively by cytology and FCM. The median time taken for sample processing was 47 min. The concordance was achieved in 84% (27/32) of samples. FCM detected FCC in 17 peritoneal lavage fluids, of which only 12 were reported positive by cytology. Five cases that had a FCC burden of less than 0.01% were reported negative by cytology. FCC with programmed death-ligand 1 (PD-L1) expression of greater than 50% was noted in 12 cases. Intra-operative FCM improves the detection of FCC in peritoneal lavage fluid compared to cytology. Due to higher sensitivity, flow cytometry offers a promising adjuvant to cytology and helps in deciding on judicious radical CCR.</p>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of monoclonal antibody to CD59 for the diagnosis of paroxysmal nocturnal hemoglobinuria by flow cytometry.","authors":"Nikiforova Kseniya Alexandrovna, Kapranov Nikolai Mikhailovich, Davydova Yulia Olegovna, Fidarova Zalina Taymurazovna, Galtseva Irina Vladimirovna, Parovnichnikova Elena Nikolaevna","doi":"10.1002/cyto.b.22237","DOIUrl":"https://doi.org/10.1002/cyto.b.22237","url":null,"abstract":"","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of circulating tumor cells is achieved by flow cytometry in melanoma patients.","authors":"Ludivine Fourdrain, Théo Brochet, Valentin Clichet, Guillaume Chaby, Brigitte Gubler, Loïc Garçon, Jean-Philippe Arnault, Thomas Boyer","doi":"10.1002/cyto.b.22236","DOIUrl":"https://doi.org/10.1002/cyto.b.22236","url":null,"abstract":"<p><p>Melanoma is an aggressive skin tumor whose incidence is rising sharply, and for which the determination of new prognostic factors is a major challenge. In oncology, circulating tumor cells (CTCs) are at the heart of much research, as they represent a source of tumor material obtained non-invasively by liquid biopsy. With this in mind, this prospective, longitudinal study looked at the detection of CTCs in melanoma patients using the flow cytometry technique, and constitutes a proof-of-principle study, as molecular biology is the most widely used technique today to detect CTCs. The labeling strategy showed high sensitivity and specificity for melanoma cells. All 35 patients in the cohort presented at least one CTC at inclusion, demonstrating that the cells circulate regardless of the stage of the disease. However, a significant increase in the number of CTCs was observed in metastatic stages compared with non-metastatic stages. With regard to the main prognostic factors for melanoma, no significant association was found between the number of CTCs and Breslow thickness or the presence of ulceration. This study must be continued in order to increase the size of the sample, with a more consistent longitudinal follow-up, in order to gain a better understanding of the prognostic significance of CTCs.</p>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}