在多中心研究中实施嗜碱性粒细胞活化检测的可能替代策略

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Pénélope Bourgoin, Thomas Dupont, Chantal Agabriel, Ania Carsin, Aurélie Verles, Maciej Cabanski, Alessandra Vitaliti, Jean-Marc Busnel
{"title":"在多中心研究中实施嗜碱性粒细胞活化检测的可能替代策略","authors":"Pénélope Bourgoin,&nbsp;Thomas Dupont,&nbsp;Chantal Agabriel,&nbsp;Ania Carsin,&nbsp;Aurélie Verles,&nbsp;Maciej Cabanski,&nbsp;Alessandra Vitaliti,&nbsp;Jean-Marc Busnel","doi":"10.1002/cyto.b.22172","DOIUrl":null,"url":null,"abstract":"<p>The Basophil Activation Test (BAT) enables flow cytometry characterization of basophil reactivity against specific allergenic molecules. The focus now revolves around democratizing this tool, but, as blood sample stability could be challenging, after having developed a simplified approach, herein, we aimed to characterize two strategies for implementing BAT in multicentric studies: store and ship blood before or after sample processing. Fresh heparin- and EDTA-anticoagulated whole blood samples followed both BAT workflows: “collect, store, process &amp; analyze” or “collect, process, store &amp; analyze”. Storage temperatures of 18–25 °C or 2–8 °C and preservation times from 0 to 7 days were considered. Interleukin-3 was also evaluated. With the “collect, store, process &amp; analyze” workflow, heparin-anticoagulated blood and 18–25 °C storage were better than other conditions. While remaining possible, basophil activation exhibited a possible reactivity decay after 24 h. Under the conditions tested, interleukin-3 had no role in enhancing basophil reactivity after storage. Conversely, the “collect, process, store &amp; analyze” workflow demonstrated that either heparin- or EDTA-anticoagulated blood can be processed and kept up to 7 days at 18–25 °C or 2–8 °C before being analyzed. Various strategies can be implemented to integrate BAT in multicentric studies. The “collect, store, process &amp; analyze” workflow remains a simplified logistical approach, but depending on time required to ship from the clinical centers to the reference laboratories, it might not be applicable, or should be used with caution. The “collect, process, store &amp; analyze” workflow may constitute a workflow improvement to provide significant flexibility without impact on basophil reactivity.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Possible alternative strategies to implement basophil activation testing in multicentric studies\",\"authors\":\"Pénélope Bourgoin,&nbsp;Thomas Dupont,&nbsp;Chantal Agabriel,&nbsp;Ania Carsin,&nbsp;Aurélie Verles,&nbsp;Maciej Cabanski,&nbsp;Alessandra Vitaliti,&nbsp;Jean-Marc Busnel\",\"doi\":\"10.1002/cyto.b.22172\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The Basophil Activation Test (BAT) enables flow cytometry characterization of basophil reactivity against specific allergenic molecules. The focus now revolves around democratizing this tool, but, as blood sample stability could be challenging, after having developed a simplified approach, herein, we aimed to characterize two strategies for implementing BAT in multicentric studies: store and ship blood before or after sample processing. Fresh heparin- and EDTA-anticoagulated whole blood samples followed both BAT workflows: “collect, store, process &amp; analyze” or “collect, process, store &amp; analyze”. Storage temperatures of 18–25 °C or 2–8 °C and preservation times from 0 to 7 days were considered. Interleukin-3 was also evaluated. With the “collect, store, process &amp; analyze” workflow, heparin-anticoagulated blood and 18–25 °C storage were better than other conditions. While remaining possible, basophil activation exhibited a possible reactivity decay after 24 h. Under the conditions tested, interleukin-3 had no role in enhancing basophil reactivity after storage. Conversely, the “collect, process, store &amp; analyze” workflow demonstrated that either heparin- or EDTA-anticoagulated blood can be processed and kept up to 7 days at 18–25 °C or 2–8 °C before being analyzed. Various strategies can be implemented to integrate BAT in multicentric studies. The “collect, store, process &amp; analyze” workflow remains a simplified logistical approach, but depending on time required to ship from the clinical centers to the reference laboratories, it might not be applicable, or should be used with caution. The “collect, process, store &amp; analyze” workflow may constitute a workflow improvement to provide significant flexibility without impact on basophil reactivity.</p>\",\"PeriodicalId\":2,\"journal\":{\"name\":\"ACS Applied Bio Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-04-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Bio Materials\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/cyto.b.22172\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cyto.b.22172","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0

摘要

嗜碱性粒细胞活化测试(BAT)可通过流式细胞术鉴定嗜碱性粒细胞对特定过敏原分子的反应性。目前的重点是使这一工具平民化,但由于血液样本的稳定性可能具有挑战性,在开发出简化方法后,我们在本文中旨在描述在多中心研究中实施嗜碱性粒细胞活化测试的两种策略:在样本处理之前或之后储存和运输血液。新鲜肝素和 EDTA 抗凝全血样本均遵循两种 BAT 工作流程:"采集、储存、处理和分析 "或 "采集、处理、储存和分析"。储存温度为 18-25 °C 或 2-8 °C,保存时间为 0-7 天。还对白细胞介素-3 进行了评估。在 "收集、储存、处理和分析 "的工作流程中,肝素抗凝血液和 18-25 ° C 的储存条件优于其他条件。在所测试的条件下,白细胞介素-3 在储存后不会增强嗜碱性粒细胞的反应性。相反,"收集、处理、储存& 分析 "的工作流程表明,肝素或 EDTA 抗凝血都可以处理,并在分析前在 18-25 °C 或 2-8 °C 下保存长达 7 天。在多中心研究中,可以采用多种策略整合 BAT。收集、储存、处理和分析 "工作流程仍然是一种简化的后勤方法,但根据从临床中心运送到参考实验室所需的时间,这种方法可能并不适用,或应谨慎使用。收集、处理、储存和分析 "工作流程可能是对工作流程的一种改进,可在不影响嗜碱性粒细胞反应性的情况下提供极大的灵活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Possible alternative strategies to implement basophil activation testing in multicentric studies

The Basophil Activation Test (BAT) enables flow cytometry characterization of basophil reactivity against specific allergenic molecules. The focus now revolves around democratizing this tool, but, as blood sample stability could be challenging, after having developed a simplified approach, herein, we aimed to characterize two strategies for implementing BAT in multicentric studies: store and ship blood before or after sample processing. Fresh heparin- and EDTA-anticoagulated whole blood samples followed both BAT workflows: “collect, store, process & analyze” or “collect, process, store & analyze”. Storage temperatures of 18–25 °C or 2–8 °C and preservation times from 0 to 7 days were considered. Interleukin-3 was also evaluated. With the “collect, store, process & analyze” workflow, heparin-anticoagulated blood and 18–25 °C storage were better than other conditions. While remaining possible, basophil activation exhibited a possible reactivity decay after 24 h. Under the conditions tested, interleukin-3 had no role in enhancing basophil reactivity after storage. Conversely, the “collect, process, store & analyze” workflow demonstrated that either heparin- or EDTA-anticoagulated blood can be processed and kept up to 7 days at 18–25 °C or 2–8 °C before being analyzed. Various strategies can be implemented to integrate BAT in multicentric studies. The “collect, store, process & analyze” workflow remains a simplified logistical approach, but depending on time required to ship from the clinical centers to the reference laboratories, it might not be applicable, or should be used with caution. The “collect, process, store & analyze” workflow may constitute a workflow improvement to provide significant flexibility without impact on basophil reactivity.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信