Cytometry Part B: Clinical Cytometry最新文献

{"title":"Immunophenotypic characteristics of plasma cells in POEMS syndrome","authors":"Zhenhua Wang,&nbsp;Tianling Ding,&nbsp;Beiqian Wu,&nbsp;Qiang Niu,&nbsp;Leyao Gu,&nbsp;Yan Yuan,&nbsp;Ping Zhu","doi":"10.1002/cytob.70012","DOIUrl":"10.1002/cytob.70012","url":null,"abstract":"<p>To analyze the immunophenotypic characteristics of plasma cells in patients with polyneuropathy, organomegaly, endocrinopathy, myeloma protein, and skin changes (POEMS) syndrome. This retrospective study included patients with POEMS syndrome hospitalized in the Department of Hematology, Huashan Hospital, from September 2017 to August 2025. Demographic data, clinical features, flow cytometry results, and bone marrow morphological findings were analyzed. A total of 69 POEMS syndrome patients (44 males, 25 females; median age 54 years) were included. Flow cytometry detected monoclonal plasma cells in 63.77% (44/69) of patients, with higher plasma cell proportions in cases with detectable monoclonal plasma cells group (<i>p =</i> 0.003). The nine-color flow cytometry protocol demonstrated a significantly higher detection rate of monoclonal plasma cells compared to the six-color protocol (77.42% vs. 52.63%, <i>p =</i> 0.033). Immunophenotypic analysis revealed 100% expression of CD38 and CD138, with higher expression of CD27, CD28, CD117, and CD81 in POEMS patients compared to multiple myeloma patients (all <i>p &lt;</i> 0.05). Bone marrow morphology showed higher plasma cell proportions in the abnormal group (<i>p =</i> 0.01). With additional fluorescent channels and enhanced detection sensitivity offering simultaneous analysis of multiple surface markers and intracellular light chains (κ/λ), nine-color flow cytometry enables more precise identification of abnormal plasma cells in POEMS syndrome based on distinct marker expression profiles. CD27, CD28, and CD117 are recommended as potential immunomarkers for flow cytometric analysis in POEMS syndrome.</p>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"110 4","pages":"266-273"},"PeriodicalIF":2.9,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cytob.70012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146200480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment on “An unusual pattern observed upon the addition of CD79b to a flow-Cytometry B-cell Lymphoma panel” (Cytometry B Clin Cytom. 2025 Jul 14. Doi: 10.1002/cyto.b.22246. Epub ahead of print. PMID: 40657818)","authors":"Aaruni Khanolkar,&nbsp;Aisha Ahmed","doi":"10.1002/cytob.70000","DOIUrl":"10.1002/cytob.70000","url":null,"abstract":"","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"110 4","pages":"297-298"},"PeriodicalIF":2.9,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145602939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Signal without noise: Practical antibody titration for platelet flow cytometry","authors":"Benjamin E. J. Spurgeon","doi":"10.1002/cytob.70004","DOIUrl":"10.1002/cytob.70004","url":null,"abstract":"<p>Antibody titration is essential for optimizing platelet flow cytometry, a technique widely used to evaluate platelet phenotype, activation status, and function. This manuscript outlines practical approaches for platelet antibody titration in whole blood, with tailored strategies for constitutive and inducible markers. It emphasizes the use of appropriate controls, consideration of marker coexpression, and selection of subsaturating antibody concentrations to maximize signal resolution while minimizing background and artifactual activation. Quantitative metrics such as the stain index and separation index are introduced as tools for evaluating staining performance. The discussion also addresses key technical variables, including combinatorial titration, spillover spreading, lot variability, and antibody-induced activation. Titration under final assay conditions is recommended to ensure reproducibility and biological relevance. These strategies provide a foundation for developing robust, high-resolution platelet assays that support both research and clinical applications, particularly as flow cytometry evolves toward greater automation and standardization.</p>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"110 4","pages":"240-248"},"PeriodicalIF":2.9,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145780612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishing a flow cytometry-based tissue receptor occupancy assay for an anti-oncostatin M receptor beta subunit therapeutic candidate in inflammatory bowel disease","authors":"Divya Jha,&nbsp;Hailey Garma,&nbsp;Sean Lear,&nbsp;Aaron Schroeder,&nbsp;Olusegun Onabajo,&nbsp;Franklin Fuh,&nbsp;William E. O'Gorman,&nbsp;Matthew D. Taylor,&nbsp;Jacqueline McBride,&nbsp;Margaret Neighbors,&nbsp;Saurabh Mehandru","doi":"10.1002/cyto.b.70025","DOIUrl":"10.1002/cyto.b.70025","url":null,"abstract":"<p>This article details the development of a tissue-based receptor occupancy (RO) assay using colonic pinch biopsies to enable the evaluation of vixarelimab target coverage in a Phase 1c study for inflammatory bowel disease (IBD). Vixarelimab, a monoclonal antibody targeting Oncostatin M Receptor Beta subunit (OSMRβ), has demonstrated clinical efficacy in treating inflammatory conditions such as prurigo nodularis and chronic pruritus. It was more recently studied in patients with ulcerative colitis (UC). Understanding the drug's distribution and retention in gut tissues is essential for assessing its therapeutic potential for IBD. While RO assays are powerful tools for evaluating drug target engagement, there are gaps in best practices for their development, especially when applied to sample types not readily available as single-cell suspensions. This article addresses challenges associated with tissue processing, cell recovery, and accurate interpretation of RO results, offering insights into overcoming limitations in sample availability and variability in drug-target interactions during assay development.</p>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"110 4","pages":"274-287"},"PeriodicalIF":2.9,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue highlights—July 2026","authors":"","doi":"10.1002/cyto.b.70049","DOIUrl":"https://doi.org/10.1002/cyto.b.70049","url":null,"abstract":"","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"110 4","pages":"237-239"},"PeriodicalIF":2.9,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148386618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"To fret over FRET: Unintended fluorescence energy transfer artifacts and their implications for flow cytometry panel design and interpretation as highlighted by TRBC1/2 assays","authors":"Hao-Wei Wang,&nbsp;Dana Delgado Colon,&nbsp;Kyle Murphy,&nbsp;Richard R. Sochacki,&nbsp;Robert Honec,&nbsp;Jacob Wellek,&nbsp;Isabella Bristol,&nbsp;Jung Jang,&nbsp;Truc Ho,&nbsp;Mariela Monreal,&nbsp;Constance M. Yuan","doi":"10.1002/cyto.b.70032","DOIUrl":"10.1002/cyto.b.70032","url":null,"abstract":"<p>Förster resonance energy transfer (FRET) serves as the fundamental mechanism underlying a wide array of technologies employed in biomedical research and clinical diagnostic applications. However, unintended FRET is often overlooked, despite its potential to introduce assay artifacts that may lead to misinterpretation. Here, we examine the impact of unwanted FRET on clinical flow cytometric testing, focusing on the T-cell assay incorporating TRBC1 and TRBC2, which has emerged as a powerful method for assessing T-cell clonality. We illustrated the effect using a representative case of spurious antigen expression associated with false TRBC restriction and demonstrate through control experiments that this artifact arises when target antigens in close proximity are labeled with fluorochromes having overlapping spectra. We quantified the spectral overlap integral J(λ), and generated a matrix for commonly used fluorochromes, providing a practical tool to identify fluorochrome pairs with a high FRET propensity that should be avoided when labeling closely situated antigens. Overall, recognizing the potential effects of unintended FRET and implementing strategies to prevent or minimize its impact are essential for ensuring accurate interpretation of clinical flow cytometric assays.</p>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"110 4","pages":"249-258"},"PeriodicalIF":2.9,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147638178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Authors' Response to Letter from Khanolkar and Ahmed concerning our article “An unusual pattern observed upon the addition of CD79b to a flow cytometry B-cell lymphoma panel” (Panakkal et al., 2025). Cytometry Part B: Clinical Cytometry. DOI: 10.1002/cyto.b.22246. PMID: 40657818","authors":"Vandana Panakkal,&nbsp;Raniah Al Amri,&nbsp;Stacey Mamatas,&nbsp;Sara A. Monaghan,&nbsp;Ahmad Al-Attar","doi":"10.1002/cytob.70005","DOIUrl":"10.1002/cytob.70005","url":null,"abstract":"","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"110 4","pages":"299-300"},"PeriodicalIF":2.9,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145827158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapy-related B-lymphoblastic leukemia/lymphoma with MYC rearrangement and multisite involvement following chemotherapy for ovarian cancer","authors":"Gailing Zhang,&nbsp;Man Chen,&nbsp;Ting Li,&nbsp;Ping Wu,&nbsp;Lina Zhang,&nbsp;Aixian Wang,&nbsp;Hui Wang","doi":"10.1002/cytob.70013","DOIUrl":"10.1002/cytob.70013","url":null,"abstract":"","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"110 4","pages":"305-308"},"PeriodicalIF":2.9,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146092400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantifying interpretive contributions to analytical variability in clinical flow cytometry.","authors":"Paul E Mead, Baptiste Labarthe, Christy Embrey, Jeffery M Klco, Kamila Czechowska","doi":"10.1002/cyto.b.70048","DOIUrl":"https://doi.org/10.1002/cyto.b.70048","url":null,"abstract":"<p><p>Reproducibility in clinical flow cytometry is essential for diagnosis, longitudinal monitoring, and interlaboratory harmonization, particularly when threshold-based lymphocyte measurements such as absolute CD4 counts guide clinical management. Although standardization of instruments, reagents, antibody panels, and acquisition protocols has reduced variability at the level of signal generation, variability introduced by human interpretation during manual gating has not been quantitatively separated from biological and technical sources. Here, we applied a two-stage workflow audit anchored to a fixed, invariant automated analysis used as an analytical reference to quantify interpretive variability in routine clinical TBNK (T-cell, B-cell, and natural killer cell) flow cytometry and to assess its impact on CD4 decision-band classification. Standardized quality-control materials and 320 consecutive clinical samples, independently analyzed by six technologists on three harmonized cytometers, were used to define an empirical analytical performance envelope (i.e., the expected range of performance) and to evaluate departures from this envelope under routine conditions. Under quality-control conditions, automated-manual differences were stable and interpretable: percentage-based endpoints showed near-zero bias with narrow dispersion, absolute counts exhibited small, consistent offsets, and CD4 decision-band assignment was preserved. In contrast, routine clinical samples showed substantially greater dispersion that was structured primarily by operator identity rather than instrument configuration. Operator-associated disagreement exceeded instrument-associated variability, was low dimensional and reproducible, and was concentrated near established CD4 thresholds, yielding discordant but adjacent decision-band classifications. These findings quantify a previously unmeasured source of analytical variability in routine hematology testing that can affect threshold-based clinical classification despite acceptable quality-control performance.</p>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148293799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantifying gating heterogeneity in the Ogata score: Implications for reproducibility and score-based diagnostics.","authors":"Marc Sorigue, Milos Miljkovic, Jordi Petriz","doi":"10.1002/cyto.b.70047","DOIUrl":"https://doi.org/10.1002/cyto.b.70047","url":null,"abstract":"","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148293810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}