A comprehensive 26-color immunophenotyping panel to study the role of the gut-liver axis in chronic liver diseases

IF 2.3 3区医学 Q3 MEDICAL LABORATORY TECHNOLOGY

Cytometry Part B: Clinical Cytometry Pub Date : 2024-09-09 DOI:10.1002/cyto.b.22203

Alix Bruneau, Yaroslava Shevchenko, Frank Tacke, Linda Hammerich

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Abstract

The gut-liver axis includes the bidirectional communication between the gut and the liver, and thus covers signals from liver-to-gut and from gut-to-liver. Disruptions of the gut-liver axis have been associated with the progression of chronic liver diseases, including alcohol-related and metabolic dysfunction-associated steatotic liver disease and cholangiopathies. Immune cells and their expression of pattern recognition receptors, activation markers or immune checkpoints might play an active role in the communication between gut and liver. Here, we present a 26-color full spectrum flow cytometry panel for human cells to decipher the role of circulating immune cells in gut-liver communication during the progression of chronic liver diseases in a non-invasive manner, which has been optimized to be used on patient-derived whole blood samples, the most abundantly available clinical material. Our panel focuses on changes in pattern recognition receptors, including toll-like receptors (TLRs) or Dectin-1, and also includes other immunomodulatory molecules such as bile acid receptors and checkpoint molecules. Moreover, this panel can be utilized to follow the progression of chronic liver diseases and could be used as a tool to evaluate the efficiency of therapeutic targets directed against microbial mediators or modulating immune cell activation.

Abstract Image

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用于研究肠肝轴在慢性肝病中的作用的 26 色免疫分型综合面板

肠肝轴包括肠道和肝脏之间的双向交流，因此涵盖了从肝脏到肠道以及从肠道到肝脏的信号。肠肝轴的破坏与慢性肝病的进展有关，包括与酒精相关的和代谢功能障碍相关的脂肪肝和胆道疾病。免疫细胞及其模式识别受体、活化标记或免疫检查点的表达可能在肠道与肝脏之间的交流中发挥着积极作用。在这里，我们展示了一种 26 色全谱流式细胞仪人体细胞检测板，以非侵入性的方式解密循环免疫细胞在慢性肝病进展过程中肠道与肝脏沟通中的作用，该检测板经过优化，可用于患者全血样本（最丰富的临床材料）。我们的检测板侧重于模式识别受体的变化，包括收费样受体（TLR）或 Dectin-1，还包括胆汁酸受体和检查点分子等其他免疫调节分子。此外，该面板还可用于跟踪慢性肝病的进展，并可用作评估针对微生物介质或调节免疫细胞活化的治疗靶点效率的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytometry Part B: Clinical Cytometry 医学-病理学

CiteScore

6.80

自引率

32.40%

发文量

审稿时长

>12 weeks

期刊介绍： Cytometry Part B: Clinical Cytometry features original research reports, in-depth reviews and special issues that directly relate to and palpably impact clinical flow, mass and image-based cytometry. These may include clinical and translational investigations important in the diagnostic, prognostic and therapeutic management of patients. Thus, we welcome research papers from various disciplines related [but not limited to] hematopathologists, hematologists, immunologists and cell biologists with clinically relevant and innovative studies investigating individual-cell analytics and/or separations. In addition to the types of papers indicated above, we also welcome Letters to the Editor, describing case reports or important medical or technical topics relevant to our readership without the length and depth of a full original report.