Current protein & peptide science最新文献_第7页

Transforming Medicine: Advances in Gene Therapy, Immunotherapy, and Targeted Cures. 改变医学：基因治疗、免疫治疗和靶向治疗的进展。

IF 1.9 4区生物学

Current protein & peptide science Pub Date : 2025-01-01 DOI: 10.2174/0113892037336137250102104842

Komal Gupta, Vikram Sharma, Tohfa Siddiqui

{"title":"Transforming Medicine: Advances in Gene Therapy, Immunotherapy, and Targeted Cures.","authors":"Komal Gupta, Vikram Sharma, Tohfa Siddiqui","doi":"10.2174/0113892037336137250102104842","DOIUrl":"10.2174/0113892037336137250102104842","url":null,"abstract":"In recent years, novel therapeutic approaches have revolutionized the landscape of medicine, offering promising avenues for the cure of various diseases. The novel approaches explore advancements in gene therapy in pharmaceuticals, immunotherapy, RNA-based therapeutics, cell-based therapies, and targeted tumor therapies. Gene therapy has emerged as a groundbreaking approach, leveraging genetic material to cure or prevent diseases by targeting defective genes. In pharmaceuticals, gene therapy holds immense potential for addressing genetic disorders, offering a personalized approach to medicine. Immunotherapy, on the other hand, harnesses the body's immune system to combat diseases, including tumors, by enhancing immune responses or directly targeting malignant cells. RNA-based therapeutics have gained prominence due to their ability to modulate gene expression, offering targeted and precise interventions for a wide range of diseases. Cell-based therapies involve the transplantation or manipulation of cells to restore or enhance their function, offering innovative solutions for diseases such as neurodegenerative disorders and cardiovascular diseases. Furthermore, targeted tumor therapies have revolutionized tumor cure by specifically targeting molecular alterations driving tumor growth and minimizing damage to healthy cells. Overall, these novel therapeutic approaches represent a paradigm shift in medicine, offering tailored and precise interventions with the potential to significantly improve patient outcomes and quality of life.","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":"436-450"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143058334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification, Characterization, and Antimicrobial Activity Against Candida parapsilosis and Staphylococcus aureus of a Highly Stable Type-1 Cystatin from Terminalia catappa L. Seeds. 高稳定型半胱抑素的纯化、鉴定及对假丝酵母菌和金黄色葡萄球菌的抑菌活性研究

IF 1.9 4区生物学

Current protein & peptide science Pub Date : 2025-01-01 DOI: 10.2174/0113892037339021241017084509

Amanda M A Moura, Jose Tadeu A Oliveira, Daniele O B Sousa, Lucas P Dias, Nadine M S Araujo, Raquel de O Rocha, Tawanny K B Aguiar, Joao M M Neto, Viviane O Silva, Ricardo M Feitosa, Queilane L S G Chaves, Marcio V Ramos, Cleverson D T Freitas

{"title":"Purification, Characterization, and Antimicrobial Activity Against Candida parapsilosis and Staphylococcus aureus of a Highly Stable Type-1 Cystatin from Terminalia catappa L. Seeds.","authors":"Amanda M A Moura, Jose Tadeu A Oliveira, Daniele O B Sousa, Lucas P Dias, Nadine M S Araujo, Raquel de O Rocha, Tawanny K B Aguiar, Joao M M Neto, Viviane O Silva, Ricardo M Feitosa, Queilane L S G Chaves, Marcio V Ramos, Cleverson D T Freitas","doi":"10.2174/0113892037339021241017084509","DOIUrl":"10.2174/0113892037339021241017084509","url":null,"abstract":"Introduction: Clinic infections caused by various microorganisms are a public health concern. The rise of new strains resistant to traditional antibiotics has exacerbated the problem. Thus, the search for new antimicrobial molecules remains highly relevant.Methods: The current study purified, characterized, and assessed the antimicrobial activity of a papain inhibitor from Terminalia catappa L. seeds.Results: The inhibitor was purified by heating the crude extract at 80°C for 30 min, followed by ion exchange chromatography on a DEAE cellulose column. The purification index was 9-fold, yielding 2.3%. SDS-PAGE and size exclusion chromatography revealed that the protease inhibitor (TcPI) is a 15.9 kDa monomeric protein. The inhibition kinetics showed that TcPI is a competitive inhibitor specific to papain (Ki = 1.02 x 10-4 M). TcPI remained active even after heating at 100oC for 120 min and at pH conditions varying from 2.0 to 10.0. Even after 60 min, TcPI was resistant to papain proteolysis. TcPI exhibited antimicrobial activity against Candida parapsilosis and Staphylococcus aureus. Conclusion: Here, we show that TcPI is a highly stable type-1 cystatin with the potential to combat infections caused by C. parapsilosis and S. aureus. Additional investigations into TcPI's structural aspects and mechanism of action, as well as safety assessments, are essential prerequisites for its potential application as a novel therapeutic intervention.","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":"308-319"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cysteine Peptidases of Calotropis procera (Apocynaceae): A Literature Review on their Biochemical Properties and Potential Applications. 夹竹桃科半胱氨酸肽酶的生物化学性质及其应用前景

IF 1.9 4区生物学

Current protein & peptide science Pub Date : 2025-01-01 DOI: 10.2174/0113892037340497241024095749

Beatriz Leite Nogueira, Filipe de Abreu Vieira, Thalles Barbosa Grangeiro

{"title":"Cysteine Peptidases of Calotropis procera (Apocynaceae): A Literature Review on their Biochemical Properties and Potential Applications.","authors":"Beatriz Leite Nogueira, Filipe de Abreu Vieira, Thalles Barbosa Grangeiro","doi":"10.2174/0113892037340497241024095749","DOIUrl":"10.2174/0113892037340497241024095749","url":null,"abstract":"The latex of the xerophytic plant Calotropis procera, popularly known as giant milkweed, contains a complex mixture of secondary metabolites and proteins and has attracted the attention of many researchers. Several bioactive laticifer enzymes from C. procera have been studied for their potential applications in the medical, agricultural and food industries. The present work aimed to review the current scientific knowledge on cysteine peptidases from the latex of this plant, highlighting their biochemical properties and possible uses as biotechnological tools. Bibliographic databases (PubMed, Scopus and Web of Science) were searched for scientific works published in the last six decades reporting the purification, biochemical characterization, molecular cloning and potential applications of laticifer cysteine peptidases from C. procera. Since the first works published in the late 1960s on the occurrence of thiol peptidases in this species, five cysteine peptidases (procerain, procerain B, CpCP-1, CpCP-2 and CpCP-3) have been purified and biochemically characterized. The characterized enzymes are members of the subfamily C1A of sulfhydryl proteases, showing the characteristic biochemical and structural features of papain and related proteins. Several biological activities of the purified enzymes have been demonstrated, including the inhibition of phytopathogenic fungi and milk coagulation properties, which may be of practical use. Moreover, pharmacologically active propeptides released from the posttranslational processing of C. procera cysteine peptidase zymogens have been shown to be promising therapeutic agents against cancer cells. Further research is needed to provide a better comprehensive understanding of the mode of action and biosafety of these molecules.","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":"296-307"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic Analysis of the Caleosin Family in Theaceae Reveals Lineagespecific Evolutionary Patterns. 对山茶科 Caleosin 家族的基因组分析揭示了特定世系的进化模式。

IF 1.9 4区生物学

Current protein & peptide science Pub Date : 2025-01-01 DOI: 10.2174/0113892037321073240828051039

Zaibao Zhang, Tao Xiong, Tianyu Fan

{"title":"Genomic Analysis of the Caleosin Family in Theaceae Reveals Lineagespecific Evolutionary Patterns.","authors":"Zaibao Zhang, Tao Xiong, Tianyu Fan","doi":"10.2174/0113892037321073240828051039","DOIUrl":"10.2174/0113892037321073240828051039","url":null,"abstract":"Introduction: Caleosins are recognized as the key proteins found in Lipid Droplets (LDs) and are crucial for the creation, maintenance, and breakdown of LDs. Nevertheless, our understanding of caleosins remains limited within Theaceae, a prominent botanical family encompassing economically significant tea and oil tea species.Methods: In this research, we conducted a comprehensive genome-wide exploration and examination of the caleosin family in Theaceae species with sequenced genomes. The gene number of caleosin was similar among Theaceae species. Segmental duplication was the main form of caleosin expansion in Shuchazao (SCZ), Huangdan (HD), Biyun (BY), Tieguanyin (TGY), Longjing (LJ), C. lanceoleosa (Cla) and C. chekiangoleosa (CCH). Synteny analysis revealed one-to-more and more-to-one collinear relationships of caleosin genes among Theaceae species.Results: Caleosins in Theaceae are categorized into either the H-family or the L-family, each exhibiting distinct motif structures and physicochemical properties. Expression analysis revealed an apparent flower-predominant expression pattern of caleosin genes in Theaceae species. In addition, most paralogous pairs displayed expression divergence.Conclusion: This research enhanced our understanding of the lineage-specific evolution of caleosin genes in Theaceae, and is valuable for future functional analysis of this gene family in tea and oil-tea species.","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":"139-155"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of Sulfanilamide-diazo Derivatives Incorporating Benzoic Acid Moieties as Novel Inhibitors of Human Carbonic Anhydrase II Activity. 含有苯甲酸基团的磺胺类重氮衍生物作为人类碳酸酐酶II活性的新抑制剂的发现。

IF 1.9 4区生物学

Current protein & peptide science Pub Date : 2025-01-01 DOI: 10.2174/0113892037332139241008054602

Farshid Belani, Maryam Mehrabi, Hadi Adibi, Masomeh Mehrabi, Reza Khodarahmi

{"title":"Discovery of Sulfanilamide-diazo Derivatives Incorporating Benzoic Acid Moieties as Novel Inhibitors of Human Carbonic Anhydrase II Activity.","authors":"Farshid Belani, Maryam Mehrabi, Hadi Adibi, Masomeh Mehrabi, Reza Khodarahmi","doi":"10.2174/0113892037332139241008054602","DOIUrl":"10.2174/0113892037332139241008054602","url":null,"abstract":"Background: Sulfonamides are widely used carbonic anhydrase inhibitors (CAIs) in clinical settings, however, their nonspecific inhibition of multiple carbonic anhydrase isoforms can lead to reduced efficacy and side effects. This study aimed to develop sulfanilamide-diazo derivatives incorporating benzoic acid moieties as novel inhibitors of hCA II activity to reduce side effects and enhance selectivity for different CA isozymes.Methods: We investigated the interaction between these derivatives and the hCA II isozyme via various spectroscopic and docking methods.Results: The kinetic data demonstrates that compound 1 (C1) and compound 2 (C2) share a similar inhibitory strength against hCA II, effectively inhibiting its esterase activity through a noncompetitive mechanism with Ki values at low micromolar levels. Fluorescence measurements indicated that the synthesized compounds suppressed the inherent fluorescence of hCA II via a static quenching process, with each compound showing a singular binding site within the enzyme. Thermodynamic evidences highlight the significance of van der Waals interactions and hydrogen bonding in the binding process. The results of molecular docking indicated that both C1 and C2 effectively obstruct the entrance to hCA II's active site, with no significant differences in their binding conformations.Conclusion: While C1 and C2 exhibit CA inhibitory potency lower than that of sulfonamide compounds, this study offers valuable insights that could pave the way for the development of a promising scaffold for designing new carbonic anhydrase inhibitors.","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":"226-240"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-vitro, In-silico Investigations Reveals Potential Cytotoxic Activity of Fermentation Metabolites from Actinomycetes Isolated from Lonar Soda Lake Against HeLa Cancer Cell Lines. 体外，计算机研究揭示了从Lonar Soda湖分离的放线菌发酵代谢产物对HeLa癌细胞的潜在细胞毒活性。

IF 1.9 4区生物学

Current protein & peptide science Pub Date : 2025-01-01 DOI: 10.2174/0113892037334392241216074545

Pradip Bawane, Santosh Yele

{"title":"In-vitro, In-silico Investigations Reveals Potential Cytotoxic Activity of Fermentation Metabolites from Actinomycetes Isolated from Lonar Soda Lake Against HeLa Cancer Cell Lines.","authors":"Pradip Bawane, Santosh Yele","doi":"10.2174/0113892037334392241216074545","DOIUrl":"10.2174/0113892037334392241216074545","url":null,"abstract":"Background: Actinomycetes, Gram-positive bacteria, are recognized for producing bioactive metabolites. Lonar Soda Lake, an alkaline ecosystem, hosts diverse actinomycetes with possible anticancer activities.Aim: To assess the cytotoxic potential of fermentation metabolites from actinomycetes isolated from Lonar Soda Lake against HeLa cancer cells employing in-vitro and in-silico methods.Objectives: Evaluate the cytotoxicity of fermentation metabolites from Lonar Lake actinomycetes on HeLa cells. Execute molecular docking to forecast metabolite connections with cancer-related proteins.Materials and methods: The actinomycetes were isolated from the sediment sample of Lonar Lake using a selective medium and recognized by gene sequencing. Cytotoxicity on HeLa cells was assessed using the MTT assay, in consort with oxidative stress and apoptotic markers (GSH, MDA, TNF-α, and caspase 3). Molecular docking and molecular dynamics studies evaluated metabolite binding to cancer-related proteins (Bcl-2, TNF-α, caspase 3).Results: Fermentation metabolites of three Lonar Lake Sediment isolates (LLSD), LLSD-5, LLSD- 7, and LLSD-9 showing promising cytotoxic activity against HeLa cell lines by MTT assay, also significantly modulate the oxidative stress parameters (GSH, MDA), and cell apoptotic marker (TNF-α, caspase 3). IC50 values were 34.17 μM (LLSD-5), 53.85 μM (LLSD-7), and 69.54 μM (LLSD-9). Furthermore, molecular docking displayed robust binding affinities to cancer-related proteins, uncovering the possible mechanism of action.Conclusion: The fermentation metabolites actinomycete isolates from Lonar Lake exhibit significant cytotoxic activity against HeLa cancer cell lines. Both in-vitro and in-silico analyses support the potential of these metabolites as anticancer agents.","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":"378-391"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diet-induced Obesity: Pathophysiology, Consequences and Target Specific Therapeutic Strategies. 饮食引起的肥胖症：病理生理学、后果和针对性治疗策略。

IF 1.9 4区生物学

Current protein & peptide science Pub Date : 2025-01-01 DOI: 10.2174/0113892037329528240827180820

Munmun Banerjee, Veda P Pandey

引用次数: 0

Advances in Migraine Treatment: A Comprehensive Clinical Review. 偏头痛治疗进展：综合临床综述。

IF 1.9 4区生物学

Current protein & peptide science Pub Date : 2025-01-01 DOI: 10.2174/0113892037329429241123095325

Rapuru Rushendran, Chitra Vellapandian

{"title":"Advances in Migraine Treatment: A Comprehensive Clinical Review.","authors":"Rapuru Rushendran, Chitra Vellapandian","doi":"10.2174/0113892037329429241123095325","DOIUrl":"10.2174/0113892037329429241123095325","url":null,"abstract":"Migraine is a neurological disease that, while not inherently causing \"chronic headaches,\" can evolve into a chronic condition over time including major symptoms such as nausea, and light, sound, and allodynia, particularly in cases of frequent episodic migraine or due to factors such as medication overuse or inadequate management. This condition's complex pathophysiology makes treatment difficult. Genetics, trigeminovascular system activation, and cortical spreading depression are involved. Epidemiological research estimates that one in seven persons worldwide are affected, mostly women. Migraine prevalence has increased dramatically in recent decades; however, it varies by demographic and location. This review covers pharmaceutical and non-pharmacological migraine therapy methods and their future. Second-generation triptans have reduced side effects and administration issues, however, Zolmitriptan and Sumatriptan still treat migraines. Monoclonal antibodies that target calcitonin gene-related peptides may prevent migraines; however, their accessibility and safety are problems. Antiepileptics, beta-blockers, and neuromodulation devices are also available. Wearable technology offers customized monitoring and intervention. Precision medicine and gene-based medicines provide hope for tailored migraine treatments, but access, privacy, and informed consent raises ethical concerns. Stakeholder engagement must promote patient autonomy and well-being, responsible implementation, and equal access to novel therapies. A holistic and multidisciplinary approach is needed to manage migraines, taking into consideration present and future therapy developments and new challenges. Research, collaboration, and ethics can improve migraine outcomes and quality of life.","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":"422-435"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myotoxicity of Crotoxin on C2C12 Myoblasts and its Inhibition by Crotalus Neutralizing Factor versus Enhanced Resistance in Myotubes: Exploring Toxicity and Membrane Potential. 克罗托毒素对 C2C12 肌母细胞的肌毒性及其被克罗托毒素中和因子抑制与肌管抵抗力增强的关系：探索毒性和膜电位。

IF 1.9 4区生物学

Current protein & peptide science Pub Date : 2025-01-01 DOI: 10.2174/0113892037317894240926081324

Maria Naiara Macedo Tavares, Micaela de Melo Cordeiro Eulalio, Hallison Mota Santana, Charles Nunes Boeno, Valdison Pereira Dos Reis, Cristina Matiele Alves Rego, Alex Augusto Ferreira E Ferreira, Mauro Valentino Paloschi, Andreimar M Soares, Consuelo Latorre Fortes-Dias, Sulamita Silva Setúbal, Juliana Pavan Zuliani

{"title":"Myotoxicity of Crotoxin on C2C12 Myoblasts and its Inhibition by Crotalus Neutralizing Factor versus Enhanced Resistance in Myotubes: Exploring Toxicity and Membrane Potential.","authors":"Maria Naiara Macedo Tavares, Micaela de Melo Cordeiro Eulalio, Hallison Mota Santana, Charles Nunes Boeno, Valdison Pereira Dos Reis, Cristina Matiele Alves Rego, Alex Augusto Ferreira E Ferreira, Mauro Valentino Paloschi, Andreimar M Soares, Consuelo Latorre Fortes-Dias, Sulamita Silva Setúbal, Juliana Pavan Zuliani","doi":"10.2174/0113892037317894240926081324","DOIUrl":"10.2174/0113892037317894240926081324","url":null,"abstract":"Background: Crotalus Neutralizing Factor (CNF) is a γ-type Phospholipase A2 (PLA2) inhibitor present in the blood of Crotalus durissus terrificus snake. Particularly, CNF inhibits the toxic action of Crotoxin (CTX), which is a major neurotoxin found in C. d. terrificus venom. CTX induces also myotoxic action and demonstrates high selectivity for skeletal muscle fibers. Consequently, CTX can diffuse beyond the site of infection, which can potentially evoke rhabdomyolysis. The present study has evaluated the effects of CTX on myoblasts and myotubes of muscle cells C2C12 in vitro and the effect of CNF on CTX-induced damage.Methods: Cytotoxicity assays were performed by measuring the mitochondrial enzyme dehydrogenase levels. Furthermore, creatine kinase and lactate dehydrogenase levels were used as indicators of muscle damage.Results: Crotoxin has been found to have cytotoxic effects on C2C12 myoblast cells, while CNF has not shown toxic effects on these cells. Furthermore, the findings have shown CNF (50 μg/mL) to abolish CTX toxicity in myoblasts. The myotubes, differentiated cells, showed no change in mitochondrial respiration when exposed to CNF or CTX, showing greater resistance to the toxic actions of crotoxin.Conclusion: The data have confirmed the potential of CNF as an anti-myotoxic agent to prevent CTX-damaged myoblasts and increase resistance to the toxic effects of crotoxin on differentiated cells.","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":"352-364"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of the Amyotrophic Lateral Sclerosis-related Q108P Mutation on the Structural Ensemble Characteristics of CHCHD10. 肌萎缩侧索硬化症相关 Q108P 突变对 CHCHD10 结构组合特征的影响

IF 1.9 4区生物学

Current protein & peptide science Pub Date : 2025-01-01 DOI: 10.2174/0113892037335036241007043530

Hakan Alici, Vladimir N Uversky, David E Kang, Junga Alexa Woo, Orkid Coskuner-Weber

{"title":"Effects of the Amyotrophic Lateral Sclerosis-related Q108P Mutation on the Structural Ensemble Characteristics of CHCHD10.","authors":"Hakan Alici, Vladimir N Uversky, David E Kang, Junga Alexa Woo, Orkid Coskuner-Weber","doi":"10.2174/0113892037335036241007043530","DOIUrl":"10.2174/0113892037335036241007043530","url":null,"abstract":"Introduction: The Q108P pathological variant of the mitochondrial Coiled-Coil-Helix-- Coiled-Coil-Helix Domain-Containing Protein 10 (CHCHD10) has been implicated in amyotrophic lateral sclerosis (ALS). Both the wild-type and CHCHD10Q108P proteins exhibit intrinsically disordered regions, posing challenges for structural studies with conventional experimental tools.Methods: This study presents the foundational characterization of the structural features of CHCHD10Q108P and compares them with those of the wild-type counterpart. We conducted multiple run molecular dynamics simulations and bioinformatics analyses.Results: Our findings reveal distinct differences in structural properties, free energy surfaces, and the outputs of principal component analysis between these two proteins. These results contribute significantly to the comprehension of CHCHD10 and its Q108P variant in terms of pathology, biochemistry, and structural biology.Conclusion: The reported structural properties hold promise for informing the development of more effective treatments for ALS.","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":"201-212"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0