Current Issues in Molecular Biology最新文献_第3页

Epigenetic Mechanisms in CRSwNP: The Role of MicroRNAs as Potential Biomarkers and Therapeutic Targets.

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-10 DOI: 10.3390/cimb47020114

Alkmini Gatsounia, Georgios Schinas, Gerasimos Danielides, Katerina Grafanaki, Nicholas Mastronikolis, Constantinos Stathopoulos, Spyridon Lygeros

{"title":"Epigenetic Mechanisms in CRSwNP: The Role of MicroRNAs as Potential Biomarkers and Therapeutic Targets.","authors":"Alkmini Gatsounia, Georgios Schinas, Gerasimos Danielides, Katerina Grafanaki, Nicholas Mastronikolis, Constantinos Stathopoulos, Spyridon Lygeros","doi":"10.3390/cimb47020114","DOIUrl":"10.3390/cimb47020114","url":null,"abstract":"Chronic rhinosinusitis with nasal polyps (CRSwNP) is a prevalent inflammatory disease of the upper airway, contributing significantly to the global disease burden. CRSwNP is characterized by sustained and exaggerated inflammation, accompanied by marked changes in gene and protein expression regulated through intricate molecular mechanisms. MicroRNAs (miRNAs), small single-stranded RNA molecules that regulate gene expression at transcriptional and post-transcriptional levels, have emerged as pivotal players in CRSwNP pathophysiology. Dysregulated miRNA expression is implicated in numerous human diseases, including cancer, asthma, and inflammatory disorders, highlighting their broad clinical relevance. In CRSwNP, miRNAs influence important inflammatory pathways, including T2 immune responses and epithelial-mesenchymal transition (EMT), which leads to chronic inflammation and tissue remodeling. Profiling studies have identified specific miRNAs as potential biomarkers for disease severity, prognosis, and therapeutic response, offering a pathway to personalized medicine. Furthermore, advances in small extracellular vesicles (sEVs) and exosomes, which naturally transport miRNAs, provide innovative avenues for targeted miRNA delivery, minimizing systemic side effects. This review explores current knowledge on miRNA expression and function in CRSwNP, emphasizing their role in disease pathogenesis and their potential as biomarkers and therapeutic targets.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Folliculogenesis: A Cellular Crosstalk Mechanism.

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-10 DOI: 10.3390/cimb47020113

Bianca Viviana Orozco-Galindo, Blanca Sánchez-Ramírez, Cynthia Lizeth González-Trevizo, Beatriz Castro-Valenzuela, Luis Varela-Rodríguez, M Eduviges Burrola-Barraza

引用次数: 0

The Derivative Difluoroboranyl-Fluoroquinolone "7a" Generates Effective Inhibition Against the S. aureus Strain in a Murine Model of Acute Pneumonia.

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-10 DOI: 10.3390/cimb47020110

L Angel Veyna-Hurtado, Hiram Hernández-López, Fuensanta Del Rocío Reyes-Escobedo, Denisse de Loera, Salvador García-Cruz, Lorena Troncoso-Vázquez, Marisol Galván-Valencia, Julio E Castañeda-Delgado, Alberto Rafael Cervantes-Villagrana

{"title":"The Derivative Difluoroboranyl-Fluoroquinolone \"7a\" Generates Effective Inhibition Against the S. aureus Strain in a Murine Model of Acute Pneumonia.","authors":"L Angel Veyna-Hurtado, Hiram Hernández-López, Fuensanta Del Rocío Reyes-Escobedo, Denisse de Loera, Salvador García-Cruz, Lorena Troncoso-Vázquez, Marisol Galván-Valencia, Julio E Castañeda-Delgado, Alberto Rafael Cervantes-Villagrana","doi":"10.3390/cimb47020110","DOIUrl":"10.3390/cimb47020110","url":null,"abstract":"During the last decades, most bacterial strains have become increasingly resistant to antibiotics. This led the WHO to declare a global emergency in 2017 and urge the development of new active compounds. Some families of antibiotics still show high antibacterial efficacy, as is the case of fluoroquinolones, which have a broad spectrum of action. For this reason, our research group derived several compounds from fluoroquinolones, selecting a compound with good antibacterial activity for further evaluations, a difluoroboranil-fluoroquinolone complex labeled 7a. Antibacterial activity was evaluated using the Kirby-Bauer method against S. aureus (clinical isolate HGZ2201#ID). The MIC and MBC were obtained by macrodilutions and reseeding. In vivo antimicrobial activity was evaluated in a Balb/c mouse model infected intratracheally with S. aureus and subsequently treated with ciprofloxacin or 7a (80 mg/kg/day) for five days. A mean of 8.55 ± 0.395 cm2 inhibition area was observed using 7a, while ciprofloxacin generated a mean inhibition of 7.86 ± 0.231 cm2. Compound 7a showed a MIC and MBC of 0.25 μg/mL. This reduced the generation of pneumonic lung tissue to 5.83%, while the untreated infected group generated 60.51% of pneumonic tissue. Compound 7a proved to be an antimicrobial agent capable of inhibiting the in vitro development of S. aureus. Furthermore, 7a showed effectiveness in decreasing the progression of acute pneumonia induced by S. aureus in a murine model.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854692/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic Approaches in Myeloid Sarcoma.

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-10 DOI: 10.3390/cimb47020111

Elzbieta Patkowska, Agnieszka Krzywdzinska, Iwona Solarska, Magdalena Wojtas, Monika Prochorec-Sobieszek

{"title":"Diagnostic Approaches in Myeloid Sarcoma.","authors":"Elzbieta Patkowska, Agnieszka Krzywdzinska, Iwona Solarska, Magdalena Wojtas, Monika Prochorec-Sobieszek","doi":"10.3390/cimb47020111","DOIUrl":"10.3390/cimb47020111","url":null,"abstract":"Myeloid sarcoma (MS), or extramedullary acute myeloid leukaemia tumour (eAML), is a rare hematopoietic neoplasm. Recognised as a distinct entity within acute myeloid leukaemia (AML), MS presents significant diagnostic challenges due to its rarity, clinical heterogeneity, and variable immunophenotypic and genetic characteristics. The mechanisms by which leukaemic stem cells (LSCs) migrate to form solid tumours in extramedullary (EM) sites remain unclear. MS can occur de novo, precede AML, and manifest alongside AML relapse. It can also develop with myelodysplastic syndromes (MDSs) or myeloproliferative neoplasms (MPNs). MS frequently presents in organs such as the skin, lymph nodes, gastrointestinal (GI) tract, and central nervous system (CNS), often resulting in diverse clinical manifestations. Diagnosis relies on a comprehensive approach, including tissue biopsy, bone marrow (BM) evaluation, and advanced imaging modalities. Accurate diagnosis is crucial for risk stratification and treatment selection. Prognosis is influenced by several factors: MS's anatomical location, timing of MS diagnosis, genetic profile, and possible treatment. This review emphasises the need for comprehensive diagnostic methods to better define individual MS characteristics and prognosis. It explores the role of novel targeted therapies in improving patient outcomes and further highlights the critical need for future multicentre data collection to optimise diagnostic and therapeutic approaches.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Introduction to Special Issue: Genomic Analysis of Common Disease.

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-10 DOI: 10.3390/cimb47020112

Golder N Wilson

引用次数: 0

Molecular Biology: Challenges and Opportunities.

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-09 DOI: 10.3390/cimb47020109

Madhav Bhatia

引用次数: 0

Activation of the Coagulation Cascade as a Universal Danger Sign.

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-09 DOI: 10.3390/cimb47020108

Eleonora A Starikova, Jennet T Mammedova, Artem A Rubinstein, Alexey V Sokolov, Igor V Kudryavtsev

{"title":"Activation of the Coagulation Cascade as a Universal Danger Sign.","authors":"Eleonora A Starikova, Jennet T Mammedova, Artem A Rubinstein, Alexey V Sokolov, Igor V Kudryavtsev","doi":"10.3390/cimb47020108","DOIUrl":"10.3390/cimb47020108","url":null,"abstract":"Hemostasis is a mechanism that stops bleeding from an injured vessel, involves multiple interlinked steps, culminating in the formation of a \"clot\" sealing the damaged area. Moreover, it has long been recognized that inflammation also provokes the activation of the coagulation system. However, there has been an increasing amount of evidence revealing the immune function of the hemostasis system. This review collects and analyzes the results of the experimental studies and data from clinical observations confirming the inflammatory function of hemostasis. Here, we summarize the latest knowledge of the pathways in immune system activation under the influence of coagulation factors. The data analyzed allow us to consider the components of hemostasis as receptors recognizing «foreign» or damaged «self» or/and as «self» damage signals that initiate and reinforce inflammation and affect the direction of the adaptive immune response. To sum up, the findings collected in the review allow us to classify the coagulation factors, such as Damage-Associated Molecular Patterns that break down the conventional concepts of the coagulation system.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial Genome Characteristics and Comparative Genomic Analysis of Spartina alterniflora.

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-08 DOI: 10.3390/cimb47020107

Hong Zhu, Chunlei Yue, Hepeng Li

{"title":"Mitochondrial Genome Characteristics and Comparative Genomic Analysis of Spartina alterniflora.","authors":"Hong Zhu, Chunlei Yue, Hepeng Li","doi":"10.3390/cimb47020107","DOIUrl":"10.3390/cimb47020107","url":null,"abstract":"The mitochondrial genome of Spartina alterniflora, an invasive species with significant ecological and economic impacts, was analyzed to provide a theoretical basis for understanding its phylogenetic relationships and molecular biology. Mitochondrial genome sequences of S. alterniflora and 23 related species from NCBI were utilized for bioinformatics and comparative genomic analyses. A sliding window analysis identified three genes (rps2, atp9, and nad6) as potential DNA barcodes for species identification. Intracellular gene transfer (IGT) events between mitochondrial and chloroplast genome were detected, highlighting the dynamic nature of genomic evolution. A selective pressure analysis revealed that most protein-coding genes (PCGs) underwent purifying selection (Ka/Ks < 1), while the nad2 and ccmB genes showed signs of positive selection pressure (Ka/Ks > 1), indicating their role in adaptation. A phylogenetic analysis demonstrated a close relationship between S. alterniflora and Eleusine indica, supported by a collinearity analysis, which suggests environmental convergence. This study provides novel insights into the structural and evolutionary characteristics of the S. alterniflora mitochondrial genome, offering valuable genomic resources for future research on invasive species management and evolutionary biology.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854523/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements in the Pathogenesis, Diagnosis, and Therapeutic Implications of Intestinal Bacteria.

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-08 DOI: 10.3390/cimb47020106

Duofei Lu, Xianxiong Ma, Kaixiong Tao, Hongwei Lei

{"title":"Advancements in the Pathogenesis, Diagnosis, and Therapeutic Implications of Intestinal Bacteria.","authors":"Duofei Lu, Xianxiong Ma, Kaixiong Tao, Hongwei Lei","doi":"10.3390/cimb47020106","DOIUrl":"10.3390/cimb47020106","url":null,"abstract":"Intestinal bacteria form one of the most complex microbial communities in the human body, playing a crucial role in maintaining host health and contributing to the development of various diseases. Here, we provide a comprehensive overview of the composition and function of intestinal bacteria, the factors affecting their homeostasis, and their association and mechanisms with a range of diseases (e.g., inflammatory bowel diseases, colorectal cancer, metabolic diseases). Additionally, their advanced potential in disease diagnosis and treatment is highlighted. Therapies, such as chemotherapy, radiotherapy, and immunotherapy, are significantly impacted by intestinal bacteria, with research indicating that bacteria can enhance chemoimmunotherapy efficiency by affecting T cell recruitment and immune cell infiltration. Fecal microbiota transplantation has emerged as a promising option for treating recurrent Clostridium difficile infections and certain metabolic and neurological disorders. Gut bacteria-related serum metabolites serve as non-invasive indicators for diagnosing CRC, while fecal immunochemical tests offer promising applications in CRC screening. Future research is needed to better understand the causal relationships between intestinal bacteria and diseases, develop more precise diagnostic tools, and evaluate the effectiveness and safety of microbiome-targeted therapies in clinical treatment. This study provides deeper insights into the role of intestinal bacteria in human health and disease, providing a scientific basis for innovative therapeutic strategies that have the potential to transform the landscape of healthcare.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the Effects and Mechanism of Corilagin on A2780 Cell Apoptosis. 研究柯里拉京对 A2780 细胞凋亡的影响和机制

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-07 DOI: 10.3390/cimb47020105

Ziyang Xu, Yuhan Jiang, Tiantian Shan, Lei Hu, Minrui Wu, Hanxu Ji, Longjie Li, Yang Yi, Hongxun Wang, Limei Wang

{"title":"Study on the Effects and Mechanism of Corilagin on A2780 Cell Apoptosis.","authors":"Ziyang Xu, Yuhan Jiang, Tiantian Shan, Lei Hu, Minrui Wu, Hanxu Ji, Longjie Li, Yang Yi, Hongxun Wang, Limei Wang","doi":"10.3390/cimb47020105","DOIUrl":"10.3390/cimb47020105","url":null,"abstract":"Previous studies have demonstrated corilagin's inhibitory effects on the growth of various cancer cells. Given the limited research on corilagin's impact on ovarian cancer, a particularly deadly gynecological malignancy, this study aimed to investigate corilagin's influence on A2780 ovarian cancer cell apoptosis and its underlying mechanisms. The goal was to evaluate corilagin's potential as a therapeutic agent for ovarian cancer. The results of the CCK-8 assay showed that corilagin inhibited the proliferation of A2780 ovarian cancer cells while exhibiting lower toxicity to normal ovarian surface epithelial cells (IOSE-80). We found that corilagin significantly altered the A2780 cell cycle, decreasing the proportion of cells in the G0/G1 and G2/M phases and inducing cell cycle arrest in the S phase. At low concentrations, corilagin induced apoptosis in A2780 cells, accompanied by a decline in mitochondrial membrane potential and calcium influx. Transcriptome sequencing analysis identified differentially expressed apoptosis-related genes in corilagin-treated A2780 cells, primarily within the PI3K-AKT pathway. Furthermore, qPCR and Western blot results confirmed the upregulation of p53 and Bax genes and the downregulation of BCL-2. Corilagin also increased the expression of apoptotic factors caspase-9, caspase-3, PUMA, and cytochrome C, indicating its ability to induce apoptosis. Overall, corilagin effectively inhibited A2780 cell proliferation, induced cell cycle arrest, and triggered apoptosis. Its anti-tumor effect in vitro suggests its potential as a therapeutic agent for ovarian cancer A2780, especially through the PI3K/p53 pathway.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0