发布求助
文献互助 智能选刊 最新文献

Current opinion in biotechnology最新文献

筛选
英文 中文
Polyphosphate: a cellular Swiss army knife 聚磷酸盐：蜂窝瑞士军刀
IF 7.1 2区 工程技术
Current opinion in biotechnology Pub Date : 2025-04-12 DOI: 10.1016/j.copbio.2025.103303
Akash Rai , Ursula Jakob
{"title":"Polyphosphate: a cellular Swiss army knife","authors":"Akash Rai ,&nbsp;Ursula Jakob","doi":"10.1016/j.copbio.2025.103303","DOIUrl":"10.1016/j.copbio.2025.103303","url":null,"abstract":"<div><div>Inorganic polyphosphate (polyP) is a ubiquitous biopolymer whose functional repertoire has rapidly expanded over the past few years. How polyP controls these seemingly unrelated functions, which range from stress resistance, motility, and DNA damage control in bacteria to blood clotting, cancer and neurodegeneration in mammals, remains largely unknown. Here, we review what is known about its synthesis and degradation pathways in mammalian cells, provide an overview over the cell compartment–specific roles of polyP, and focus on recent studies, which showed that many of polyP’s activities appear to be mediated by its ability to either solubilize, scaffold, or phase separate proteins. Future studies will show how polyP achieves these vastly different effects on proteins and hence controls its many functions.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"93 ","pages":"Article 103303"},"PeriodicalIF":7.1,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corrigendum to “Production of therapeutic glycoproteins in glycoengineered plant: old farm for new crops” [Curr Opin Biotechnol 87 (2024) 103145] “糖工程植物中治疗性糖蛋白的生产：新作物的旧农场”的勘误[Curr Opin biotechnology 87 (2024) 103145]
IF 7.1 2区 工程技术
Current opinion in biotechnology Pub Date : 2025-04-06 DOI: 10.1016/j.copbio.2025.103302
Mu-Rong Kao , Rebecka Karmakar Saldivar , Yves S Y Hsieh
{"title":"Corrigendum to “Production of therapeutic glycoproteins in glycoengineered plant: old farm for new crops” [Curr Opin Biotechnol 87 (2024) 103145]","authors":"Mu-Rong Kao ,&nbsp;Rebecka Karmakar Saldivar ,&nbsp;Yves S Y Hsieh","doi":"10.1016/j.copbio.2025.103302","DOIUrl":"10.1016/j.copbio.2025.103302","url":null,"abstract":"","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"93 ","pages":"Article 103302"},"PeriodicalIF":7.1,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Redesigning and rethinking genetic circuits: the potential of transcriptional rewiring in filamentous fungi 重新设计和重新思考遗传回路：丝状真菌转录重新布线的潜力
IF 7.1 2区 工程技术
Current opinion in biotechnology Pub Date : 2025-04-02 DOI: 10.1016/j.copbio.2025.103301
Alejandra Goity , Luis F Larrondo
{"title":"Redesigning and rethinking genetic circuits: the potential of transcriptional rewiring in filamentous fungi","authors":"Alejandra Goity ,&nbsp;Luis F Larrondo","doi":"10.1016/j.copbio.2025.103301","DOIUrl":"10.1016/j.copbio.2025.103301","url":null,"abstract":"<div><div>Fungi play vital roles in ecosystems and biotechnology, yet synthetic biology efforts have predominantly focused on the model organism <em>Saccharomyces cerevisiae</em>, with less attention given to filamentous fungi. This review explores the molecular toolkits available for gene regulatory network (GRN) manipulation in these organisms, focusing on transcriptional rewiring and promoter engineering. Beyond constitutive and inducer-responsive promoters, the use of <em>cis</em>-elements responsive to physical cues like light and temperature enables gene expression control with unprecedented spatiotemporal precision. Additionally, newly available modular toolkits further facilitate GRN rewiring for metabolic engineering and fundamental research in filamentous fungi. Using <em>Neurospora crassa</em> as a model, we illustrate how transcriptional rewiring enhances cellulase production and reveals design principles of complex circuits, such as circadian oscillators.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"93 ","pages":"Article 103301"},"PeriodicalIF":7.1,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Engineering bacterial microcompartments to enable sustainable microbial bioproduction from C1 greenhouse gases 工程细菌微室，使可持续的微生物生产从C1温室气体
IF 7.1 2区 工程技术
Current opinion in biotechnology Pub Date : 2025-03-29 DOI: 10.1016/j.copbio.2025.103299
Elizabeth R Johnson , Madeline R Joseph , Danielle Tullman-Ercek
{"title":"Engineering bacterial microcompartments to enable sustainable microbial bioproduction from C1 greenhouse gases","authors":"Elizabeth R Johnson ,&nbsp;Madeline R Joseph ,&nbsp;Danielle Tullman-Ercek","doi":"10.1016/j.copbio.2025.103299","DOIUrl":"10.1016/j.copbio.2025.103299","url":null,"abstract":"<div><div>One-carbon (C1) greenhouse gases are the primary driver of global climate change. Fermenting these gases into higher-value products is an attractive strategy for climate action and sustainable development. C1 gas-fermenting bacteria are promising chassis organisms, but various technical challenges hinder scale-up to industrial production levels. Bacterial microcompartments (MCPs), proteinaceous organelles that encapsulate enzymatic pathways, may confer several metabolic benefits to increase the industrial potential of these bacteria. Many species of gas-fermenting bacteria are already predicted to natively produce MCPs. Here, we describe how these organelles can be identified and engineered to encapsulate pathways that convert C1 gases into valuable chemical products.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"93 ","pages":"Article 103299"},"PeriodicalIF":7.1,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Acetogenic mixotrophy for carbon-neutral and carbon-negative production of chemicals 用于碳中性和碳负性化学品生产的丙酮混合营养
IF 7.1 2区 工程技术
Current opinion in biotechnology Pub Date : 2025-03-28 DOI: 10.1016/j.copbio.2025.103298
John D Hill , Hyeongmin Seo , Eleftherios T Papoutsakis
{"title":"Acetogenic mixotrophy for carbon-neutral and carbon-negative production of chemicals","authors":"John D Hill ,&nbsp;Hyeongmin Seo ,&nbsp;Eleftherios T Papoutsakis","doi":"10.1016/j.copbio.2025.103298","DOIUrl":"10.1016/j.copbio.2025.103298","url":null,"abstract":"<div><div>Microbial conversion of renewable carbon sources into valuable chemicals can significantly reduce our reliance on fossil resources and decrease the carbon footprint of chemical manufacturing. Of emerging interest is anaerobic, nonphotosynthetic mixotrophy (ANP mixotrophy) that simultaneously converts renewable carbohydrates and C<sub>1</sub> gases (CO<sub>2</sub> and CO) into value-added chemical products in carbon-neutral and even carbon-negative fermentations. Despite significant advances in ANP mixotrophy over the past decade, several challenges remain. We discuss key challenges for carbon-neutral/negative ANP fermentations, notably the necessity of supplying additional electrons for reduced metabolite production, the slow adoption of genetic tools, uncertainties about carbon catabolite repression, and gas transfer limitations.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"93 ","pages":"Article 103298"},"PeriodicalIF":7.1,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent developments of oleaginous yeasts toward sustainable biomanufacturing 产油酵母在可持续生物制造方面的最新进展
IF 7.1 2区 工程技术
Current opinion in biotechnology Pub Date : 2025-03-28 DOI: 10.1016/j.copbio.2025.103297
Paul A Adamczyk , Tian Jiang , Karuna Jetty , Vijaydev Ganesan , Di Liu
{"title":"Recent developments of oleaginous yeasts toward sustainable biomanufacturing","authors":"Paul A Adamczyk ,&nbsp;Tian Jiang ,&nbsp;Karuna Jetty ,&nbsp;Vijaydev Ganesan ,&nbsp;Di Liu","doi":"10.1016/j.copbio.2025.103297","DOIUrl":"10.1016/j.copbio.2025.103297","url":null,"abstract":"<div><div>Oleaginous yeast are remarkably versatile organisms, distinguished by their natural capacities to accumulate high levels of neutral lipids and broad substrate range. With recent growing interests in engineering non-model organisms as superior biomanufacturing platforms, oleaginous yeasts have emerged as promising chassis for oleochemicals, terpenoids, organic acids, and other valuable products. Advancement in systems biology along with genetic tool development have significantly expanded our understanding of the metabolism in these species and enabled engineering efforts to produce biofuels and bioproducts from diverse feedstocks. This review examines the latest technical advances in oleaginous yeast research toward sustainable biomanufacturing. We cover recent developments in systems biology-enabled metabolism understanding, genetic tools, feedstock utilization, and strain engineering approaches for the production of various valuable chemicals.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"93 ","pages":"Article 103297"},"PeriodicalIF":7.1,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Editorial overview: Pioneering innovations in food biotechnology 编辑概述：食品生物技术的开创性创新
IF 7.1 2区 工程技术
Current opinion in biotechnology Pub Date : 2025-03-27 DOI: 10.1016/j.copbio.2025.103300
Christoph Wittmann , Ken-Ishi Yoshida
{"title":"Editorial overview: Pioneering innovations in food biotechnology","authors":"Christoph Wittmann ,&nbsp;Ken-Ishi Yoshida","doi":"10.1016/j.copbio.2025.103300","DOIUrl":"10.1016/j.copbio.2025.103300","url":null,"abstract":"","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"93 ","pages":"Article 103300"},"PeriodicalIF":7.1,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143703981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Advancements in phosphorus species profiling and bioavailability assessment with implications for phosphorus sustainability 磷种类分析和生物利用度评估的进展及其对磷可持续性的影响
IF 7.1 2区 工程技术
Current opinion in biotechnology Pub Date : 2025-03-27 DOI: 10.1016/j.copbio.2025.103295
Nehreen Majed , Zijian Wang , Mathew T Baldwin , Saif Mahmod Foij Uddin , Pallab Dash , April Z Gu
{"title":"Advancements in phosphorus species profiling and bioavailability assessment with implications for phosphorus sustainability","authors":"Nehreen Majed ,&nbsp;Zijian Wang ,&nbsp;Mathew T Baldwin ,&nbsp;Saif Mahmod Foij Uddin ,&nbsp;Pallab Dash ,&nbsp;April Z Gu","doi":"10.1016/j.copbio.2025.103295","DOIUrl":"10.1016/j.copbio.2025.103295","url":null,"abstract":"<div><div>Phosphorus (P) is an essential nutrient that governs ecosystem productivity, drives global biogeochemical cycles, and plays a central role in water–energy–food nexus. The increasing scarcity of P reserves and their environmental losses necessitate precise detection to monitor P in all contexts for effective and sustainable resource management. This review highlights recent advances in analytical techniques for P speciation and bioavailability across environmental matrices, including both bulk and single-cell methods. Furthermore, recent emerging insights into microbial P cycling were discussed, particularly the role of polyphosphate and polyphosphate-accumulating organisms in dynamic P transformations. By emphasizing the sustainability implications of P, we stress the importance of precise and quantitative detection methods to inform sustainable P management and mitigate the global P crisis.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"93 ","pages":"Article 103295"},"PeriodicalIF":7.1,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143703979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Engineered T cells and macrophages: two arms to seize solid tumors 工程T细胞和巨噬细胞：抓住实体肿瘤的两条胳膊
IF 7.1 2区 工程技术
Current opinion in biotechnology Pub Date : 2025-03-26 DOI: 10.1016/j.copbio.2025.103296
Luigi Russo, Ilaria De Martino , Matteo Marchetti , Velia Siciliano
{"title":"Engineered T cells and macrophages: two arms to seize solid tumors","authors":"Luigi Russo,&nbsp;Ilaria De Martino ,&nbsp;Matteo Marchetti ,&nbsp;Velia Siciliano","doi":"10.1016/j.copbio.2025.103296","DOIUrl":"10.1016/j.copbio.2025.103296","url":null,"abstract":"<div><div>Following the breakthroughs of CAR T cells in the treatment of several hematological malignancies, clinical trials based on genetically modified immune cells are exponentially increasing. Redirecting T cell cytotoxicity against solid tumors via CARs, however, encountered several barriers that require the engineering of additional functions to improve safety, migration, efficacy, and persistence in solid tumors. Complementary strategies tried to harness macrophage properties such as cancer cell phagocytosis, cytokine release, and antigen presentation to induce broader antitumorigenic immune response. While providing a comprehensive overview on the latest technologies in the cell-based immunotherapy realm, we propose that engineering synthetic interplay between immune cells will be the next breakthrough to drive safer and more effective living therapeutics.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"93 ","pages":"Article 103296"},"PeriodicalIF":7.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143703980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dos and don’ts for scaling up gas fermentations 扩大气体发酵规模的注意事项。
IF 7.1 2区 工程技术
Current opinion in biotechnology Pub Date : 2025-03-20 DOI: 10.1016/j.copbio.2025.103294
Lars Puiman , Carolin Bokelmann , Sean D Simpson , Alfred M Spormann , Ralf Takors
{"title":"Dos and don’ts for scaling up gas fermentations","authors":"Lars Puiman ,&nbsp;Carolin Bokelmann ,&nbsp;Sean D Simpson ,&nbsp;Alfred M Spormann ,&nbsp;Ralf Takors","doi":"10.1016/j.copbio.2025.103294","DOIUrl":"10.1016/j.copbio.2025.103294","url":null,"abstract":"<div><div>Gas fermentation processes (using CO<sub>2</sub>, CO, H<sub>2</sub>, CH<sub>4</sub>) have gained significant research and commercial interest in the last years due to their potential for carbon capture and sequestration. The small economic margins of these processes necessitate the use of large-volume bioreactors. For cost-effective gas delivery, we advise using pneumatically agitated bioreactors, like bubble column reactors, compared to traditional stirred-tank reactors. Although scale-up is conventionally done on an empirical and rule-of-thumb basis, rational methods are currently available. The most important one is the knowledge-driven scaling-up approach, wherein (CFD-based) hydrodynamic and kinetic models of large-scale bioreactors guide the design of representative lab-scale experiments. We suggest several future research directions to enhance the predictive capacity of these models and thereby accelerate scaling-up gas fermentation processes.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"93 ","pages":"Article 103294"},"PeriodicalIF":7.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：604180095
Book学术官方微信