Current opinion in biotechnology最新文献_第2页

Emerging biosensor and assay-enabled high-throughput screening solutions for enzyme and strain engineering 用于酶和菌株工程的新兴生物传感器和高通量筛选解决方案

IF 7 2区工程技术

Current opinion in biotechnology Pub Date : 2026-04-01 Epub Date: 2026-01-27 DOI: 10.1016/j.copbio.2026.103439

Jingyun Zhang , Dan Zheng , Sheena Chan , Matthew W Chang , Chueh Loo Poh

{"title":"Emerging biosensor and assay-enabled high-throughput screening solutions for enzyme and strain engineering","authors":"Jingyun Zhang , Dan Zheng , Sheena Chan , Matthew W Chang , Chueh Loo Poh","doi":"10.1016/j.copbio.2026.103439","DOIUrl":"10.1016/j.copbio.2026.103439","url":null,"abstract":"<div><div>High-throughput screening (HTS) platforms and automated biofoundries have enabled large-scale experimentation in enzyme and microbial strain engineering. Central to HTS are biosensors and assays, which translate biochemical activities into measurable signals, enabling rapid evaluation of cellular and enzymatic performance. Yet despite advancements in high-throughput infrastructure, the limited availability of robust biosensors or assays and the difficulty of integrating them with HTS, particularly with ultra-HTS, remains a major bottleneck. This review highlights recent progress and challenges in applying biosensors- and assays-enabled HTS for enzyme and strain libraries. We discuss strategies for integrating diverse biosensor types, including transcription factors, G protein-coupled receptors, aptamers, fluorogenic RNAs, riboswitches, and colorimetric assays, with HTS to detect a broad range of metabolites and products. We also explore how biosensor-enabled HTS facilitates data generation for machine learning-guided biocatalyst engineering. Collectively, these advances accelerate biocatalyst discovery and drive the next generation of sustainable biomanufacturing.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"98 ","pages":"Article 103439"},"PeriodicalIF":7.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146090684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular biotechnology for the biodegradation of organofluorine compounds 有机氟化合物生物降解的分子生物技术。

IF 7 2区工程技术

Current opinion in biotechnology Pub Date : 2026-04-01 Epub Date: 2026-02-03 DOI: 10.1016/j.copbio.2026.103446

Lawrence P Wackett

引用次数: 0

From toxin to biofuel: engineering microbes for methanol biomanufacturing 从毒素到生物燃料：用于甲醇生物制造的工程微生物。

IF 7 2区工程技术

Current opinion in biotechnology Pub Date : 2026-04-01 Epub Date: 2026-01-28 DOI: 10.1016/j.copbio.2026.103442

Rui Hou , Xiaoxin Zhai , Yongjin J Zhou , Jiaoqi Gao

引用次数: 0

Biodesulfurization: Back on-stage through synthetic biology and metabolic engineering approaches 生物脱硫：通过合成生物学和代谢工程方法重回舞台

IF 7 2区工程技术

Current opinion in biotechnology Pub Date : 2026-04-01 Epub Date: 2026-02-12 DOI: 10.1016/j.copbio.2026.103449

Panayiotis D Glekas , Olga Martzoukou , Dimitris G Hatzinikolaou

引用次数: 0

Engineering microbial therapeutics for metabolic disorders: synthetic biology strategies and future direction 代谢紊乱的工程微生物疗法：合成生物学策略和未来方向

IF 7 2区工程技术

Current opinion in biotechnology Pub Date : 2026-04-01 Epub Date: 2026-01-26 DOI: 10.1016/j.copbio.2026.103440

Yani Li , Yanting Wang , Siyu Zhu , Zhongmin Tang

{"title":"Engineering microbial therapeutics for metabolic disorders: synthetic biology strategies and future direction","authors":"Yani Li , Yanting Wang , Siyu Zhu , Zhongmin Tang","doi":"10.1016/j.copbio.2026.103440","DOIUrl":"10.1016/j.copbio.2026.103440","url":null,"abstract":"<div><div>Metabolic disorders, including type 2 diabetes mellitus, obesity, metabolic dysfunction-associated steatohepatitis, etc., are an escalating global health challenge with substantial clinical and socio-economic burdens. Conventional pharmacological therapies are constrained by short-term efficacy, instability, and energy-intensive production. This review aims to evaluate how synthetic biology enables engineered probiotics and microbial systems as self-regulating, living therapeutics that integrate precision treatment with sustainable, bio-based production. Such systems can secrete therapeutic molecules, degrade harmful metabolites, remodel host metabolic microenvironments, and respond to physiological signals to achieve adaptive, feedback-controlled interventions. By exploiting versatile microbial hosts and low-energy fermentation, they minimize chemical waste and carbon footprint. We also discussed preclinical studies demonstrating restored glucose and lipid homeostasis, modulation of appetite, and attenuation of inflammation. Collectively, our review highlights that synthetic biology exemplifies a transformative, sustainable paradigm for metabolic disease management.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"98 ","pages":"Article 103440"},"PeriodicalIF":7.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146045251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AI-empowered crop protection against insect-borne diseases 利用人工智能进行作物保护，预防虫媒疾病

IF 7 2区工程技术

Current opinion in biotechnology Pub Date : 2026-04-01 Epub Date: 2026-01-27 DOI: 10.1016/j.copbio.2026.103445

Pingzhi Zhao , Qiudong Xia , Jian Ye

{"title":"AI-empowered crop protection against insect-borne diseases","authors":"Pingzhi Zhao , Qiudong Xia , Jian Ye","doi":"10.1016/j.copbio.2026.103445","DOIUrl":"10.1016/j.copbio.2026.103445","url":null,"abstract":"<div><div>Insect-borne plant diseases, such as psyllid-transmitted citrus Huanglongbing (HLB) and whitefly-transmitted viral diseases, pose a major threat to global agriculture. These insect-borne pathogens establish in multipartite interactions with host plants and insect vectors, frequently altering host physiology and vector behavior to facilitate transmission. This review examines recent advances in plant defense against these pathogens, as well as pathogen strategies that undermine host defenses. We emphasize the promise of artificial intelligence (AI) in accelerating scientific research and technological advancements for managing insect-borne diseases. These strategies contribute to building an integrated platform, which trains scientific foundation models and creates AI agents to automate research workflows and test hypotheses. They also provided precision intervention strategies by identifying resistance genes, developing <em>in silico</em> approaches to develop new pesticides, and synthetic immune proteins. These AI platforms for insect-borne plant diseases enable a systemic understanding of the complex pathogen–plant–insect interactions, thereby offering new opportunities for controlling disease outbreaks and epidemics.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"98 ","pages":"Article 103445"},"PeriodicalIF":7.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146090683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering the gut microbiome and its impact on human health 设计肠道微生物群及其对人类健康的影响

IF 7 2区工程技术

Current opinion in biotechnology Pub Date : 2026-02-01 Epub Date: 2025-12-18 DOI: 10.1016/j.copbio.2025.103415

Amornthep Kingkaw , Kevin Mok , Massalin Nakphaichit , Mattheos Koffas , Wanwipa Vongsangnak

引用次数: 0

Cell-free protein synthesis in microcompartments towards cell–cell communication 面向细胞间通讯的微室中无细胞蛋白合成。

IF 7 2区工程技术

Current opinion in biotechnology Pub Date : 2026-02-01 Epub Date: 2025-12-18 DOI: 10.1016/j.copbio.2025.103416

Joshua Ricouvier , Aurore Dupin , Matthaeus Schwarz-Schilling , Ferdinand Greiss , Shirley S. Daube , Roy H. Bar-Ziv

{"title":"Cell-free protein synthesis in microcompartments towards cell–cell communication","authors":"Joshua Ricouvier , Aurore Dupin , Matthaeus Schwarz-Schilling , Ferdinand Greiss , Shirley S. Daube , Roy H. Bar-Ziv","doi":"10.1016/j.copbio.2025.103416","DOIUrl":"10.1016/j.copbio.2025.103416","url":null,"abstract":"<div><div>Recent efforts in bottom-up synthetic biology focus on fabricating programmable biological units that can be viewed as synthetic cells. Combining microfluidic techniques with cell-free protein expression systems defines the geometrical limits of the synthetic cell (e.g. microfluidic compartments, droplets, vesicles) and facilitates communication pathways to distribute functions over an assembly of synthetic cells. In this review, we describe and compare the different strategies implemented to reconstitute cell–cell communication among synthetic cells. We focus especially on various experimental setups of microcompartmentalization containing a cell-free expression system and genetic material. We highlight efforts to develop and engineer different modes of communication among the synthetic cells in different forms, varying by the degree of permeability, resource renewal, stability, and scalability, and how these influence the trade-off between programmability and biomimicry. We then summarize recent progress in the realization of different stages of communication (signaling, processing, and output generation) by genetic circuits, holding great promise for applications in synthetic biology and biotechnology.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103416"},"PeriodicalIF":7.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145780600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocatalytic surfaces in architecture 建筑中的生物催化表面。

IF 7 2区工程技术

Current opinion in biotechnology Pub Date : 2026-02-01 Epub Date: 2026-01-19 DOI: 10.1016/j.copbio.2025.103435

Rachel Armstrong

{"title":"Biocatalytic surfaces in architecture","authors":"Rachel Armstrong","doi":"10.1016/j.copbio.2025.103435","DOIUrl":"10.1016/j.copbio.2025.103435","url":null,"abstract":"<div><div>This review explores the reconceptualisation of microbial colonisation on buildings: from a detrimental process (biofouling) to a source of beneficial, programmable biocatalysis. Strategies for embedding microbial and fungal communities into architectural materials to perform functions such as bioremediation, biomineralisation, and energy generation are explored. The analysis includes the multiscalar design of bioreceptive substrates, engineered living paints, mycelium composites, and probiotic surfaces, which transform passive structures into metabolically active interfaces. These approaches are regarded as <em>Engineered Eco-Ornamentation</em>, where surface design intentionally supports microbial ecology and urban metabolism. The integration of these living systems with computational modelling and digital fabrication to create adaptive building systems is considered. Key challenges include scaling biological processes for architectural application, ensuring long-term material durability, and aligning metabolic activity with practical construction constraints. Addressing these challenges positions functionally designed biocatalytic surfaces as a foundational research field for more regenerative and ecologically integrated architecture.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103435"},"PeriodicalIF":7.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146009123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances and critical evaluation of autonomous protein engineering: towards transparent, accessible, and reproducible platforms 自主蛋白工程的进展和关键评价：走向透明，可访问和可复制的平台

IF 7 2区工程技术

Current opinion in biotechnology Pub Date : 2026-02-01 Epub Date: 2025-11-28 DOI: 10.1016/j.copbio.2025.103395

Konstantin FG Weigmann, Uwe T Bornscheuer, Mark Doerr

{"title":"Advances and critical evaluation of autonomous protein engineering: towards transparent, accessible, and reproducible platforms","authors":"Konstantin FG Weigmann, Uwe T Bornscheuer, Mark Doerr","doi":"10.1016/j.copbio.2025.103395","DOIUrl":"10.1016/j.copbio.2025.103395","url":null,"abstract":"<div><div>Protein engineering aims to enhance enzymatic properties such as activity, selectivity, stability, and solvent tolerance by restructuring protein frameworks beyond natural performance limits. This process relies on the iterative Design-Build-Test-Learn cycle, where experimental feedback guides progressive improvements. Advancements in artificial intelligence have transformed both the Design and Learn phases, with zero-shot protein language models predicting beneficial mutations directly from sequence data and supervised models integrating assay results to refine subsequent variant designs. These approaches reduce the dependence on structural insights while enabling the discovery of synergistic effects across mutations. Automation technologies, including robotic liquid handlers and integrated platforms, have become central to modern protein engineering by reducing errors, ensuring reproducibility, and enabling large-scale variant screening. Emerging autonomous platforms demonstrate closed-loop optimization that couples protein library design, automated plasmid transformation/protein expression and corresponding assays, and machine learning–driven decision-making. These systems achieve significant accelerations in the research process, reducing multi-round engineering cycles from months to days while successfully improving diverse proteins/enzymes to a targeted objective. Beyond single-lab platforms, orchestration frameworks adhering to FAIR data principles and leveraging knowledge graphs promise distributed ‘self-driving’ laboratories capable of coordinating workflows across facilities. While high setup costs and proprietary systems remain challenging, open-source and modular alternatives highlight a path toward transparent, flexible automation. Collectively, these innovations are redefining protein engineering as an increasingly autonomous, data-driven discipline.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103395"},"PeriodicalIF":7.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145616654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0