Current Medical Science最新文献

{"title":"Silencing NCAPD3 Inhibits Tumor Growth and Metastasis in Hepatocellular Carcinoma by Suppressing PI3K-AKT Signalling Pathway.","authors":"Jun Lv, Fu-Yuan Gan, Ming-Hao Li, Qing-Jun Yin","doi":"10.1007/s11596-025-00026-2","DOIUrl":"10.1007/s11596-025-00026-2","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the expression pattern of non-SMC condensin II complex subunit D3 (NCAPD3) in hepatocellular carcinoma (HCC) tissues, assess its association with clinical characteristics, and explore the effects of NCAPD3 on HCC cells and the potential underlying mechanisms.</p><p><strong>Methods: </strong>NCAPD3 expression in HCC tumors and adjacent noncancerous tissues was quantified via quantitative PCR. Patients were divided into high- and low-expression groups on the basis of NCAPD3 levels, and associations with clinical parameters were assessed. The effects of NCAPD3 knockdown and the phosphatidylinositol-3-kinase (PI3K) agonist Y-P 740 on cell functions were examined via cell proliferation, Transwell migration, and invasion assays. Differentially expressed genes following NCAPD3 knockdown in SMMC-7721 cells were identified via mRNA sequencing. Western blotting was performed to measure NCAPD3, AKT serine/threonine kinase 1 (AKT1), and phosphorylated AKT1 levels.</p><p><strong>Results: </strong>NCAPD3 mRNA expression was notably upregulated in HCC tissues as compared with that in adjacent noncancer tissues. A positive correlation was observed between NCAPD3 expression and both lymphatic and distant metastases in patients with HCC. NCAPD3 knockdown reduced the proliferation and metastasis of SMMC-7721 and Huh-7 cells. mRNA sequencing revealed 140 downregulated genes and 125 upregulated genes. Further validation experiments confirmed that NCAPD3 modulated the PI3K-AKT signalling pathway and that the PI3K agonist Y-P 740 counteracted the effects of NCAPD3 knockdown.</p><p><strong>Conclusions: </strong>Elevated NCAPD3 expression was strongly correlated with HCC metastasis. NCAPD3 inhibition impedes HCC cell growth and metastatic potential by suppressing the PI3K-AKT signalling pathway.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"253-263"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Causal Links Between Gut Microbiota and Vitamin Deficiencies: Evidence from Mendelian Randomization Analysis.","authors":"Zi-Xuan Hou, Wen-Jing Li, Rong Pi, Han-Wen-Xi Wang, Meng-Na Dai, Yan Ouyang, Su-Yun Li","doi":"10.1007/s11596-025-00038-y","DOIUrl":"10.1007/s11596-025-00038-y","url":null,"abstract":"<p><strong>Objective: </strong>Vitamin deficiencies, particularly in vitamins A, B12, and D, are prevalent across populations and contribute significantly to a range of health issues. While these deficiencies are well documented, the underlying etiology remains complex. Recent studies suggest a close link between the gut microbiota and the synthesis, absorption, and metabolism of these vitamins. However, the specific causal relationships between the gut microbiota composition and vitamin deficiencies remain poorly understood. Identifying key bacterial species and understanding their role in vitamin metabolism could provide critical insights for targeted interventions.</p><p><strong>Methods: </strong>We conducted a two-sample Mendelian randomization (MR) study to assess the causal relationship between the gut microbiota and vitamin deficiencies (A, B12, D). The genome-wide association study data for vitamin deficiencies were sourced from the FinnGen biobank, and the gut microbiota data were from the MiBioGen consortium. MR analyses included inverse variance-weighted (IVW), MR‒Egger, weighted median, and weighted mode approaches. Sensitivity analyses and reverse causality assessments were performed to ensure robustness and validate the findings.</p><p><strong>Results: </strong>After FDR adjustment, vitamin B12 deficiency was associated with the class Verrucomicrobiae, order Verrucomicrobiales, family Verrucomicrobiaceae, and genus Akkermansia. Vitamin A deficiency was associated with the phylum Firmicutes and the genera Fusicatenibacter and Ruminiclostridium 6. Additional associations for vitamin B12 deficiency included the Enterobacteriaceae and Rhodospirillaceae and the genera Coprococcus 2, Lactococcus, and Ruminococcaceae UCG002. Vitamin D deficiency was associated with the genera Allisonella, Eubacterium, and Tyzzerella 3. Lachnospiraceae and Lactococcus were common risk factors for both B12 and D deficiency. Sensitivity analyses confirmed the robustness of the findings against heterogeneity and horizontal pleiotropy, and reverse MR tests indicated no evidence of reverse causality.</p><p><strong>Conclusions: </strong>Our findings reveal a possible causal relationship between specific gut microbiota characteristics and vitamin A, B12 and D deficiencies, providing a theoretical basis for addressing these nutritional deficiencies through the modulation of the gut microbiota in the future and laying the groundwork for related interventions.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"321-330"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12053135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to: Naringin and Naringenin: Potential Multi-Target Agents for Alzheimer's Disease.","authors":"Jing Lu, Jie Chen, Shu-Yue Li, Guang-Jie Pan, Yi Ou, Li-Fu Yuan, Jian-Ping Jiang, Ling-Hui Zeng, Jie Zhao","doi":"10.1007/s11596-025-00039-x","DOIUrl":"10.1007/s11596-025-00039-x","url":null,"abstract":"","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"393"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Histone Demethylase Inhibitor GSK-J4 Attenuates Periodontal Bone Loss and Inflammation in a Rat Model of Periodontitis.","authors":"Jian Kang, Huan Yu, Xu Xiang, Yong-Qiang Ma, Le Zhang, Yuan Zhang, Zhi-Tao Wang, Jing Yang, Zheng Zhang, Hui-Ru Zou, Yue Wang","doi":"10.1007/s11596-025-00018-2","DOIUrl":"10.1007/s11596-025-00018-2","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the treatment effect of the histone demethylase inhibitor GSK-J4, a small molecule that inhibits the demethylase activity of Jumonji domain-containing protein 3 (JMJD3), in the treatment of periodontitis.</p><p><strong>Methods: </strong>Gingival tissues from patients with moderate to severe chronic periodontitis and healthy controls were collected to evaluate JMJD3 expression via real-time quantitative reverse transcription PCR (RT-qPCR) and immunohistochemistry (IHC). Next, Sprague-Dawley (SD) rats were used to investigate the effect of GSK-J4 in vivo. The experimental periodontitis model was induced by upper first molar ligation and gingival sulcus injection of Porphyromonas gingivalis. The rats were divided into a healthy group, a periodontitis group, periodontitis plus GSK-J4 treatment groups (P + GSK-J4 15 mg/kg or 25 mg/kg), and a periodontitis plus dimethyl sulfoxide (DMSO) group (P + DMSO). After 4 weeks, maxillary molar segments were assessed via micro-computed tomography (CT) and hematoxylin and eosin (HE) staining. Serum tumor necrosis factor-α (TNF-α) levels were measured by enzyme-linked immunosorbent assay (ELISA).</p><p><strong>Results: </strong>Higher expression of the Jmjd3 gene and JMJD3 protein was detected in human inflamed gingiva than in healthy gingiva (P < 0.05). GSK-J4 administration reversed alveolar bone absorption [i.e., reduced alveolar bone crest (ABC)-cementoenamel junction (CEJ) distance], reduced inflammatory cell accumulation at the crest of the alveolar bone, and alleviated serum TNF-α levels in rats with periodontitis. Moreover, the number of H3K27me3-positive nuclei was greater in model rats treated with GSK J4 than in model rats.</p><p><strong>Conclusions: </strong>The histone demethylase inhibitor GSK-J4 attenuated periodontal bone loss and inflammation in a rat periodontitis model by targeting JMJD3.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"382-390"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to: Early Aerobic Exercise Promotes Neurological Function Recovery of Rats after Cerebral Ischemia/Reperfusion by Upregulating the Expression of Heat Shock Protein A5.","authors":"Zhi-Feng Peng, Nai-Bao Zhang, Jian Meng, Ji-Hong Zhang","doi":"10.1007/s11596-025-00029-z","DOIUrl":"10.1007/s11596-025-00029-z","url":null,"abstract":"","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"391-392"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NOX4 Suppresses Ferroptosis Through Regulation of the Pentose Phosphate Pathway in Colorectal Cancer.","authors":"Jing Zhu, Chao Jiang, Fan Wang, Ming-Yue Tao, Hai-Xiao Wang, Yuan Sun, Hong-Xia Hui","doi":"10.1007/s11596-025-00013-7","DOIUrl":"10.1007/s11596-025-00013-7","url":null,"abstract":"<p><strong>Objective: </strong>Nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (NOXs) are known as major sources of reactive oxygen species (ROS), yet their role in regulating cellular antioxidative metabolism and ferroptosis is unclear. This study assessed the expression and clinical relevance of NOXs across pan-cancer and investigated the role of NOX4 in colorectal cancer progression METHODS: We analyzed transcriptomic and survival data from The Cancer Genome Atlas (TCGA) for NOXs across 22 types of solid tumors. A CRISPR library targeting NOXs was developed for potential therapeutic target screening in colorectal cancer cells (CRCs). Techniques such as CRISPR-knockout cell lines, 1,2-¹³C-glucose tracing, PI staining, BrdU assays, and coimmunoprecipitation were employed to elucidate the function of NOX4 in CRCs.</p><p><strong>Results: </strong>NOX4 emerged as a key therapeutic target for colorectal cancer from TCGA data. CRISPR screening highlighted its essential role in CRC survival, with functional experiments confirming that NOX4 upregulation promotes cell survival and proliferation. The interaction of NOX4 with glucose‑6‑phosphate dehydrogenase (G6PD) was found to enhance the pentose phosphate pathway (PPP), facilitating ROS clearance and protecting CRCs against ferroptosis.</p><p><strong>Conclusions: </strong>This study identified NOX4 as a novel ferroptosis suppressor and a therapeutic target for the treatment of colorectal cancer. The findings suggest that a coupling between NADPH oxidase enzyme NOX4 and the PPP regulates ferroptosis and reveal an accompanying metabolic vulnerability for therapeutic targeting in colorectal cancer.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"264-279"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into Virus-Host Interactions: Lessons from Caenorhabditis elegans-Orsay Virus Model.","authors":"Xun Wu, Heng Liu, Yusong R Guo","doi":"10.1007/s11596-025-00004-8","DOIUrl":"10.1007/s11596-025-00004-8","url":null,"abstract":"<p><p>The study of virus-host interactions has been significantly advanced using model organisms, with nematodes being a prominent example. Caenorhabditis elegans (C. elegans) nematodes have provided valuable insights into the mechanisms of viral infections, host defense strategies, and the development of antiviral therapies. With the discovery of natural viral pathogens of nematodes, Orsay virus, Le Blanc virus, Santeuil virus, and Mělník virus, the exploration of the virus-host interaction model based on nematodes has entered a new era. The virus-host interaction network consists of viruses, hosts, and the antagonistic effects of viruses on host immunity. The nematode virus-host interaction model is a concrete manifestation used to study the complex relationships among these three elements. Previous studies have indicated that during the entire process of nematode infection by viruses, antiviral RNA interference (RNAi) plays a crucial role. Additionally, the host's innate immune responses, such as the antiviral-specific intracellular pathogen response (IPR) and certain signaling pathways homologous to those in humans, are particularly important in the natural immune and antiviral processes of nematodes. These processes are regulated by multiple genes in the host. The reverse genetics system for Orsay virus has been successfully developed to study viral gene function and virus-host interactions. Nematodes serve as simple host models for understanding RNA virus replication, related cellular components, and virus-host interaction mechanisms. These findings will likely contribute to the development of antiviral treatment strategies based on novel targets.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"169-184"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Simple Modification Results in a Significant Improvement in Measuring the Size of Extracellular Vesicles.","authors":"Xiao-Jun Liu, Zhen-Sheng Ma, Yan Li, Tai-Bing Fan, Zhen-Wei Ge, Zhi-Jun Ou, Jing-Song Ou","doi":"10.1007/s11596-025-00045-z","DOIUrl":"https://doi.org/10.1007/s11596-025-00045-z","url":null,"abstract":"<p><strong>Objective: </strong>Size distribution is an important biophysical property of extracellular vesicles (EVs). EVs include small EVs (s-EVs) and large EVs (l-EVs) by size. Differential ultracentrifugation (dUC) is widely used to separate EVs from biofluids, but it can precipitate large impurity particles. Dynamic light scattering (DLS) is a simple and fast method for analyzing the size distribution of EVs. However, this approach is nonideal for heterogeneous and polydisperse samples since a small quantity of large impurity particles can markedly disturb the DLS results. Here, we developed a simple method to improve the reliability of DLS measurements.</p><p><strong>Methods: </strong>Plasma was obtained from 13 volunteers. The plasma was first processed by dUC to obtain crude l-EVs. The crude l-EVs were filtered with syringe filters (pore size of 1 μm and membrane material of hydrophilic polyvinylidene fluoride (PVDF)) to remove large impurity particles from l-EVs. The size distributions of the crude l-EVs and filtered l-EVs were measured via DLS.</p><p><strong>Results: </strong>After the samples were filtered, the coefficients of variation of the hydrodynamic radius and Peak 1 intensity of the filtered l-EVs decreased from 20.39% (12.76-28.96%) and 20.44% (14.58-28.32%) to 3.05% (1.79-4.72%) and 3.43% (1.76-5.88%), respectively, compared with those of the crude l-EVs.</p><p><strong>Conclusion: </strong>These findings suggest that filtration can effectively separate circulating l-EVs in plasma to remove large impurity particles and make samples suitable for characterization by DLS. Our findings provide a simple method to improve precision via DLS to measure the size distribution of EVs.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":"45 2","pages":"244-252"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Efficacy of Retrograde Pubic Ramus Intramedullary Nails and Percutaneous Cannulated Screws in Treating Anterior Pelvic Ring Fractures: A Retrospective Cohort Study.","authors":"En-Zhi Yin, Xue-Feng Yuan, Yang-Xing Luo, Peng-Hui Xiang, Li He, Yi-Liu Liao, Cheng-la Yi","doi":"10.1007/s11596-025-00044-0","DOIUrl":"10.1007/s11596-025-00044-0","url":null,"abstract":"<p><strong>Objective: </strong>To compare the clinical outcomes of retrograde pubic ramus intramedullary nail (RPRIN) and percutaneous cannulated screw (PCS) in the treatment of anterior pelvic ring fractures (APRFs).</p><p><strong>Methods: </strong>This retrospective cohort study included 45 patients with APRFs treated between February 2019 and October 2022 in our trauma center. Patients were divided into two groups based on the surgical method: 20 received RPRIN fixation, and 25 received PCS fixation. Key variables including operation time, fluoroscopic time, blood loss, and postoperative complications were analyzed. Fracture reduction quality was assessed using the Matta score system, and pelvic functional recovery was evaluated using the Majeed score system at the final follow-up. Quantitative variables were compared using the independent sample t test, while categorical variables were analyzed using Chi-square and Fisher's exact tests.</p><p><strong>Results: </strong>The RPRIN group had significantly shorter operation time (36.3 ± 5.6 min vs. 49.5 ± 6.9 min, P < 0.01), fluoroscopic time (32.0 ± 2.8 s vs. 48.4 ± 3.6 s, P < 0.01), and less blood loss (20.4 ± 7.6 mL vs. 34.0 ± 5.7 mL, P < 0.01) than the PCS group. Fracture reduction quality (Matta outcome) and pelvic functional recovery (Majeed outcome) were comparable between the two groups (P > 0.05). No significant complications were reported in either group.</p><p><strong>Conclusions: </strong>Both RPRIN and PCS are effective for treating APRFs. However, RPRIN offers distinct advantages by reducing operation time, fluoroscopic time, and blood loss, making it a more efficient and less invasive option. Further multicenter studies and biomechanical analyses are warranted to confirm these findings.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"341-348"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progression from Initial Lesions to Type B Aortic Dissection: A Patient-Specific Study of Computational Fluid Dynamics Models with Follow-up Data.","authors":"Yue-Ying Pan, Zhi-Yue Guan, Chen-Wei Li, Han-Xiong Guan","doi":"10.1007/s11596-025-00006-6","DOIUrl":"10.1007/s11596-025-00006-6","url":null,"abstract":"<p><strong>Background and objective: </strong>The natural history of type B aortic intramural hematoma (IMH) is highly heterogeneous. A computational fluid dynamics (CFD) model can be utilized to calculate a range of data pertinent to flow dynamics, including flow rates, blood velocity, pressure, and wall shear stress. This study presents a series of CFD simulations that model the dynamic progression from type B aortic IMH to false lumen formation.</p><p><strong>Methods: </strong>A 66-year-old male patient presenting with chest and back pain underwent aortic computed tomography angiography (CTA), and a 3D patient-specific model was constructed. To evaluate the hemodynamic environment, the velocity, pressure, time-averaged wall shear stress (TAWSS), and oscillatory shear index (OSI) were calculated.</p><p><strong>Results: </strong>A modest quantity of slow flow and recirculation flow was observed in the vicinity of the ulcer-like protrusion (ULP). During the formation of the false lumen, low-velocity blood flow entered the false lumen and resulted in vortex flow. ULPs were located in the region with higher TAWSS, and some high OSIs were found on the ULPs.</p><p><strong>Conclusion: </strong>This preliminary study suggests a potential association between the TAWSS or OSI and progression from type B aortic IMH to aortic dissection.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"373-381"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}