Current Medical Science最新文献

{"title":"Interleukin-6: Molecular Mechanisms and Therapeutic Perspectives in Atrial Fibrillation.","authors":"Jin-Fang Yu, Qian Dong, Yi-Mei Du","doi":"10.1007/s11596-025-00021-7","DOIUrl":"10.1007/s11596-025-00021-7","url":null,"abstract":"<p><p>Atrial fibrillation (AF) is a prevalent cardiac arrhythmia with a multifactorial pathophysiology involving electrical, structural, and autonomic remodeling of the atria. AF is closely associated with elevated interleukin-6 (IL-6) levels, which contribute to atrial remodeling and the progression of AF. This review summarizes the mechanisms by which IL-6 promotes AF through inflammatory pathways, atrial fibrosis, electrical remodeling, and calcium mishandling. Experimental models have demonstrated that IL-6 neutralization reduces the incidence of AF, highlighting its potential as a therapeutic target. Future studies should focus on IL-6 blockade strategies to manage AF, aiming to improve patient outcomes.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"157-168"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Optogenetic Neuromodulation in Regulating Depression.","authors":"Jin Zhang, Xiang Peng, Man Li, Xiao-Ming Zhang, Hong-Chun Xiang","doi":"10.1007/s11596-025-00037-z","DOIUrl":"10.1007/s11596-025-00037-z","url":null,"abstract":"<p><p>Depression is a multifaceted disorder with a largely unresolved etiology influenced by a complex interplay of pathogenic factors. Despite decades of research, it remains a major condition that significantly diminishes patients' quality of life. Advances in optogenetics have introduced a powerful tool for exploring the neural mechanisms underlying depression. By selectively expressing optogenes in specific cell types in mice, researchers can study the roles of these cells through targeted light stimulation, offering new insights into central nervous system disorders. The use of viral vectors to express opsins in distinct neuronal subtypes enables precise activation or inhibition of these neurons via light. When combined with behavioral, morphological, and electrophysiological analyses, optogenetics provides an invaluable approach to investigating the neural mechanisms of psychiatric conditions. This review synthesizes current research on the application of optogenetics to understand the mechanisms of depression. This study aims to enhance our knowledge of optogenetic strategies for regulating depression and advancing antidepressant research.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"185-193"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143728960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safranal Ameliorates Renal Damage, Inflammation, and Podocyte Injury in Membranous Nephropathy via SIRT/NF-κB Signalling.","authors":"Yan Bao, Ya-Mei Ge, Zheng Wang, Hong-Yun Wang, Qiong Wang, Jun Yuan","doi":"10.1007/s11596-025-00020-8","DOIUrl":"10.1007/s11596-025-00020-8","url":null,"abstract":"<p><strong>Objective: </strong>Safranal is a natural product from saffron (Crocus sativus L.) with anti-inflammatory and nephroprotective potential. This study aimed to explore the role of safranal in a cationic bovine serum albumin (C-BSA)-induced rat model of membranous glomerulonephritis (MGN).</p><p><strong>Methods: </strong>After model establishment, Sprague-Dawley rats were administered 100 or 200 mg/kg safranal by gavage. A biochemical analyser was used to measure the urine protein levels and serum levels of renal function parameters. Hematoxylin-eosin and immunofluorescence staining of kidney tissues were performed to examine histopathological changes and assess the expression of IgG, C3, and Sirt1. Western blotting was performed to measure the protein levels of podocin, nephrin, Sirt1, and factors involved in the NF-κB/p65 pathway. Inflammatory cytokine levels in renal homogenates were determined by ELISA.</p><p><strong>Results: </strong>Safranal at 100 or 200 mg/kg reduced kidney weight (2.07 ± 0.15 g and 2.05 ± 0.15 g) and the kidney somatic index (0.83 ± 0.08% and 0.81 ± 0.08%) in MGN rats compared with those in the model group without drug administration (2.62 ± 0.17 g and 1.05 ± 0.1%). C-BSA increased the urine protein level to 117.68 ± 10.52 mg/day (compared with the sham group, 5.03 ± 0.45 mg/day), caused dysregulation of renal function indicators, and induced glomerular expansion and inflammatory cell infiltration in the rat kidney samples. All the biochemical and histological changes were improved by safranal administration. Safranal at two doses also increased the fluorescence intensities of IgG (0.1 ± 0.009 and 0.088 ± 0.008) and C3 (0.065 ± 0.006 and 0.048 ± 0.004) compared with those in the MGN group (0.15 ± 0.013 and 0.086 ± 0.008). Additionally, safranal reversed the downregulation of podocin, nephrin, and Wilms tumor protein-1 (WT1) levels and reversed the high inflammatory cytokine levels in MGN rats. Mechanistically, safranal activated Sirt1 signalling to interfere with NF-κB signalling in the kidney tissues of MGN rats.</p><p><strong>Conclusions: </strong>Safranal ameliorates renal damage, inflammation, and podocyte injury in MGN by upregulating SIRT1 and inhibiting NF-κB signalling.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"288-300"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12052800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and Safety of Third-Generation EGFR-TKIs Combined with Radiotherapy for Advanced NSCLC with Typical EGFR Mutations: A Retrospective Study.","authors":"Wen-Xuan Zhang, Hui-Chan Xue, Ye Zhao, Shuang-Bing Xu","doi":"10.1007/s11596-025-00032-4","DOIUrl":"10.1007/s11596-025-00032-4","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the efficacy and safety of third-generation epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) in combination with radiotherapy (RT) for patients with advanced non-small cell lung cancer (NSCLC) harboring typical EGFR mutations.</p><p><strong>Methods: </strong>Patients who received treatment with third-generation EGFR-TKIs alone or in combination with RT were retrospectively enrolled at a single center. The primary endpoint was progression-free survival (PFS). Differences in PFS between the two groups were assessed via the Kaplan-Meier method. Additionally, a subgroup analysis was conducted to further explore the effect of thoracic RT combined with EGFR-TKIs.</p><p><strong>Results: </strong>This study included a total of 260 patients, among whom 81 patients received third-generation EGFR-TKIs and 179 patients received third-generation EGFR-TKIs plus RT. There was a significant difference in median PFS (mPFS) (13.0 versus 18.1 months, P = 0.0003) between the two groups. Moreover, third-generation EGFR-TKIs plus thoracic RT significantly improved the mPFS (13.0 versus 23.7 months, P < 0.0001). We observed that third-generation EGFR-TKIs plus RT increased the incidence of pneumonia, but all the cases were grade 1 or 2.</p><p><strong>Conclusion: </strong>The addition of RT can delay the occurrence of acquired resistance to third-generation EGFR-TKIs, thereby significantly prolonging PFS in advanced NSCLC patients. RT for primary lung lesions exhibited a significant synergistic effect with EGFR-TKI treatment, and the adverse events of the combination therapy were acceptable.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"280-287"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Manifestations of Ocular Toxoplasmosis in Hubei, China: Case Series.","authors":"Mu Li, Xian Zhang, Xiao-Qin Yan, Peng-Cheng Li","doi":"10.1007/s11596-025-00028-0","DOIUrl":"10.1007/s11596-025-00028-0","url":null,"abstract":"<p><strong>Objective: </strong>The clinical features, disease course and visual outcomes of toxoplasmosis are less commonly reported in China than in other countries. To reduce misdiagnosis and improve visual function, the clinical characteristics, management and visual outcomes of 13 cases of ocular toxoplasmosis (OT) were described.</p><p><strong>Methods: </strong>This retrospective study included 14 eyes of 13 patients who were diagnosed with OT in Hubei, China. The clinical characteristics, course of treatment and outcomes are presented. There were 7 males and 6 females.</p><p><strong>Results: </strong>The main form of OT was retinochoroiditis with vitritis or anterior uveitis. Next-generation sequencing was applied to 3 eyes, and positive results were found in those eyes. Thirteen patients were positive for Toxoplasma gondii IgG antibodies, and 3 of them were also positive for IgM T. gondii antibodies. One patient with acquired immune deficiency syndrome was diagnosed with coinfection with OT and cytomegalovirus, as evidenced by an aqueous humor etiological test. Three patients were misdiagnosed with noninfectious uveitis. Recurrence occurred in 3 eyes during the follow-up periods. One patient who received vitreous implantation of Ozurdex therapy at another hospital before referral relapsed. One patient who received sulfadiazine, azithromycin and glucocorticoid therapy relapsed. One patient who received sulfadiazine therapy experienced relapse. Patients who received clindamycin and sulfadiazine or who received clindamycin only did not experience recurrence during the follow-up period. The best corrected visual acuity was improved in 6 eyes after inflammation resolved.</p><p><strong>Conclusions: </strong>Primary active retinochoroiditis is the main form of OT in Hubei, China. Timely correct diagnosis on the basis of ocular characteristics and aetiological test results and effective treatment should be adopted to prevent poor visual outcomes and recurrence.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"314-320"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine Learning-Based Identification of Novel Exosome-Derived Metabolic Biomarkers for the Diagnosis of Systemic Lupus Erythematosus and Differentiation of Renal Involvement.","authors":"Zhong-Yu Wang, Wen-Jing Liu, Qing-Yang Jin, Xiao-Shan Zhang, Xiao-Jie Chu, Adeel Khan, Shou-Bin Zhan, Han Shen, Ping Yang","doi":"10.1007/s11596-025-00023-5","DOIUrl":"10.1007/s11596-025-00023-5","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to investigate the exosome-derived metabolomics profiles in systemic lupus erythematosus (SLE), identify differential metabolites, and analyze their potential as diagnostic markers for SLE and lupus nephritis (LN).</p><p><strong>Methods: </strong>Totally, 91 participants were enrolled between February 2023 and January 2024 including 58 SLE patients [30 with nonrenal-SLE and 28 with Lupus nephritis (LN)] and 33 healthy controls (HC). Ultracentrifugation was used to isolate serum exosomes, which were analyzed for their metabolic profiles using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Endogenous metabolites were identified via public metabolite databases. Random Forest, Lasso regression and Support Vector Machine Recursive Feature Elimination (SVM-RFE) algorithms were employed to screen key metabolites, and a prediction model was constructed for SLE diagnosis and LN discrimination. ROC curves were constructed to determine the potential of these differential exosome-derived metabolites for the diagnosis of SLE. Furthermore, Spearman's correlation was employed to evaluate the potential links between exosome-derived metabolites and the clinical parameters which reflect disease progression.</p><p><strong>Results: </strong>A total of 586 endogenous serum exosome-derived metabolites showed differential expression, with 225 exosome-derived metabolites significantly upregulated, 88 downregulated and 273 exhibiting no notable changes in the HC and SLE groups. Machine learning algorithms revealed three differential metabolites: Pro-Asn-Gln-Met-Ser, C24:1 sphingolipid, and protoporphyrin IX, which exhibited AUC values of 0.998, 0.992 and 0.969 respectively, for distinguishing between the SLE and HC groups, with a combined AUC of 1.0. In distinguishing between the LN and SLE groups, the AUC values for these metabolites were 0.920, 0.893 and 0.865, respectively, with a combined AUC of 0.931, demonstrating excellent diagnostic performance. Spearman correlation analysis revealed that Pro-Asn-Gln-Met-Ser and protoporphyrin IX were positively correlated with the SLE Disease Activity Index (SLEDAI) scores, urinary protein/creatinine ratio (ACR) and urinary protein levels, while C24:1 sphingolipid exhibited a negative correlation.</p><p><strong>Conclusions: </strong>This study provides the first comprehensive characterization of the exosome-derived metabolites in SLE and established a promising prediction model for SLE and LN discrimination. The correlation between exosome-derived metabolites and key clinical parameters strongly indicated their potential role in SLE pathological progression.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"231-243"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicities Associated with Sacituzumab Govitecan: Data from Clinical Trials and a Real-World Pharmacovigilance Database.","authors":"Qiao-Yun Tan, Xiang-Ping Mei, Yue Hu, Hong-Ge Wu, Lin-Ka Xie, Jie Xiong, Jing Yao","doi":"10.1007/s11596-025-00030-6","DOIUrl":"10.1007/s11596-025-00030-6","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to analyze the adverse effects (AEs) of sacituzumab govitecan (SG) through multiple sources of data to provide a reference for clinical safety management.</p><p><strong>Methods: </strong>Clinical trials of SG with available safety data were retrieved and included in the pooled analysis. The adverse drug reaction (ADR) signals of SG were collected from the FDA Adverse Event Reporting System (FAERS) database. Drug interactions with SG in the DDInter database were summarized.</p><p><strong>Results: </strong>A total of 6 clinical trials involving 1737 patients were included in the pooled analysis, and the most common AEs of ≥ grade 3 were neutropenia (46%), leukopenia (13%), and anemia (8%). In the pharmacovigilance study, 1024 AE reports were extracted, and the most common toxicities of SG were hematologic and gastrointestinal. AEs not included in the drug instructions also presented high signals, such as meningitis, colitis and lymphedema. A total of 40 drugs identified could induce drug-drug interactions when they were concomitantly administered with SG.</p><p><strong>Conclusions: </strong>This study provides the most comprehensive profile of SG toxicity on the basis of data from clinical trials and the FRAES and DDInter databases. Attention should be given not only to common ADRs but also to ADRs not reported in drug instructions, and potential drugs that can induce drug-drug interactions.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"301-313"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a Multimodal 3D Atlas for a Micrometer-Scale Brain-Computer Interface Based on Mixed Reality.","authors":"Hong Zhou, Zi-Neng Yan, Wei-Hang Gao, Xiang-Xin Lv, Rui Luo, Jason Shih Hoellwarth, Lei He, Jia-Ming Yang, Jia-Yao Zhang, Hong-Lin Wang, Yi Xie, Xiao-Liang Chen, Ming-di Xue, Ying Fang, Yu-Yu Duan, Rui-Yuan Li, Xu-Dong Wang, Rui-Lin Wang, Mao Xie, Li Huang, Peng-Ran Liu, Zhe-Wei Ye","doi":"10.1007/s11596-025-00033-3","DOIUrl":"10.1007/s11596-025-00033-3","url":null,"abstract":"<p><strong>Objective: </strong>To develop a multimodal imaging atlas of a rat brain-computer interface (BCI) that incorporates brain, arterial, bone tissue and a BCI device using mixed reality (MR) for three-dimensional (3D) visualization.</p><p><strong>Methods: </strong>An invasive BCI was implanted in the left visual cortex of 4-week-old Sprague-Dawley rats. Multimodal imaging techniques, including micro-CT and 9.0 T MRI, were used to acquire images of the rat cranial bone structure, vascular distribution, brain tissue functional zones, and BCI device before and after implantation. Using 3D-slicer software, the images were fused through spatial transformations, followed by image segmentation and 3D model reconstruction. The HoloLens platform was employed for MR visualization.</p><p><strong>Results: </strong>This study constructed a multimodal imaging atlas for rats that included the skull, brain tissue, arterial tissue, and BCI device coupled with MR technology to create an interactive 3D anatomical model.</p><p><strong>Conclusions: </strong>This multimodal 3D atlas provides an objective and stable reference for exploring complex relationships between brain tissue structure and function, enhancing the understanding of the operational principles of BCIs. This is the first multimodal 3D imaging atlas related to a BCI created using Sprague-Dawley rats.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"194-205"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Durational Exposure to Particulate Matter and Changes in Fertility Intentions: A Study of Adults in China.","authors":"Jia-Yu Wang, Xin Yun, Rui Qu, Wei-Qian Zhang, Jia Liang, Yu Guan, Dong-Dong Tang, Yu Chen, Tai-Lang Yin","doi":"10.1007/s11596-025-00046-y","DOIUrl":"https://doi.org/10.1007/s11596-025-00046-y","url":null,"abstract":"<p><strong>Objective: </strong>The effects of prolonged exposure to persistently elevated atmospheric pollutants, commonly termed air pollution waves, on fertility intentions remain inadequately understood. This study aims to investigate the association between particulate matter (PM) exposure and fertility intentions.</p><p><strong>Methods: </strong>In this nationwide cross-sectional study, we analyzed data from 10,747 participants (5496 females and 5251 males). PM waves were defined as periods lasting 3‒6 consecutive days during which the daily average concentrations exceeded China's Ambient Air Quality Standards Grade II thresholds (PM_2.5 > 75 μg/m³ and PM₁₀ > 150 μg/m³). We employed multivariate logistic regression models to assess the association between exposure to PM waves and fertility intentions.</p><p><strong>Results: </strong>Significant inverse associations were detected between exposure to PM_2.5 wave events (characterized by concentrations exceeding 75 μg/m³ for durations of 4‒6 days, P < 0.05) and PM₁₀ wave events (defined as concentrations exceeding 150 μg/m³ for 6 consecutive days, P < 0.05) and fertility intentions among females. In contrast, neither the PM_2.5 wave nor the PM₁₀ wave events demonstrated statistically significant correlations with fertility intentions in males (P > 0.05 for all comparisons). The potentially susceptible subgroup was identified as females aged 20-30 years.</p><p><strong>Conclusions: </strong>Our results provide the first evidence that PM_2.5 and PM₁₀ waves are associated with a reduction in female fertility intentions, offering critical insights for the development of public health policies and strategies aimed at individual protection.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":"45 2","pages":"363-372"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silencing NCAPD3 Inhibits Tumor Growth and Metastasis in Hepatocellular Carcinoma by Suppressing PI3K-AKT Signalling Pathway.","authors":"Jun Lv, Fu-Yuan Gan, Ming-Hao Li, Qing-Jun Yin","doi":"10.1007/s11596-025-00026-2","DOIUrl":"10.1007/s11596-025-00026-2","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the expression pattern of non-SMC condensin II complex subunit D3 (NCAPD3) in hepatocellular carcinoma (HCC) tissues, assess its association with clinical characteristics, and explore the effects of NCAPD3 on HCC cells and the potential underlying mechanisms.</p><p><strong>Methods: </strong>NCAPD3 expression in HCC tumors and adjacent noncancerous tissues was quantified via quantitative PCR. Patients were divided into high- and low-expression groups on the basis of NCAPD3 levels, and associations with clinical parameters were assessed. The effects of NCAPD3 knockdown and the phosphatidylinositol-3-kinase (PI3K) agonist Y-P 740 on cell functions were examined via cell proliferation, Transwell migration, and invasion assays. Differentially expressed genes following NCAPD3 knockdown in SMMC-7721 cells were identified via mRNA sequencing. Western blotting was performed to measure NCAPD3, AKT serine/threonine kinase 1 (AKT1), and phosphorylated AKT1 levels.</p><p><strong>Results: </strong>NCAPD3 mRNA expression was notably upregulated in HCC tissues as compared with that in adjacent noncancer tissues. A positive correlation was observed between NCAPD3 expression and both lymphatic and distant metastases in patients with HCC. NCAPD3 knockdown reduced the proliferation and metastasis of SMMC-7721 and Huh-7 cells. mRNA sequencing revealed 140 downregulated genes and 125 upregulated genes. Further validation experiments confirmed that NCAPD3 modulated the PI3K-AKT signalling pathway and that the PI3K agonist Y-P 740 counteracted the effects of NCAPD3 knockdown.</p><p><strong>Conclusions: </strong>Elevated NCAPD3 expression was strongly correlated with HCC metastasis. NCAPD3 inhibition impedes HCC cell growth and metastatic potential by suppressing the PI3K-AKT signalling pathway.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"253-263"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}