IF 1.9 3区医学

Cornea Pub Date : 2025-04-01 Epub Date: 2025-01-17 DOI: 10.1097/ICO.0000000000003801

Myriam Böhm, Mohit Parekh, Neha Deshpande, Queenie Cheung, Nathan Shatz, Varun Kumar, Ula V Jurkunas

{"title":"Mitochondria-Targeted Antioxidant (MitoQ) and Nontargeted Antioxidant (Idebenone) Mitigate Mitochondrial Dysfunction in Corneal Endothelial Cells.","authors":"Myriam Böhm, Mohit Parekh, Neha Deshpande, Queenie Cheung, Nathan Shatz, Varun Kumar, Ula V Jurkunas","doi":"10.1097/ICO.0000000000003801","DOIUrl":"10.1097/ICO.0000000000003801","url":null,"abstract":"Purpose: To investigate the effectiveness of mitochondrial-targeted antioxidant mitoquinone (MitoQ) and nontargeted antioxidant idebenone (Idb) in alleviating mitochondrial dysfunction in corneal endothelial cells (CEnCs).Methods: In vitro experiments were conducted using immortalized normal human corneal endothelial cells (HCEnC-21T; SVN1-67F) and Fuchs endothelial corneal dystrophy (FECD) cells (SVF5-54F; SVF3-76M). Cells were pretreated with MitoQ or Idb and then exposed to menadione (MN) with simultaneous antioxidant treatment. Mitochondrial parameters were evaluated through adenosine triphosphate viability assays, JC-1 staining for mitochondrial membrane potential, and Tom-20 antibody staining for fragmentation, with analysis performed using ImageJ software. HCEnC-21T cells were additionally exposed to ultraviolet-A (25 J/cm 2 ) to assess drug effects under physiological stress. Mitochondrial fragmentation in FECD specimens was analyzed pre- and post-treatment with the drugs. Statistical analysis was conducted using 1-/2-way analysis of variance with post-hoc Tukey test.Results: MitoQ and Idb enhanced cell viability and mitochondrial membrane potential in both normal and FECD cells under MN-induced stress. Idb reduced MN-induced mitochondrial fragmentation by 32% more than MitoQ in HCEnC-21T cells and by 13% more in SVF5-54F cells. Under ultraviolet-A stress, Idb and MitoQ improved mitochondrial function by 31% and 25%, respectively, with MitoQ increasing mitochondrial function by 42% in FECD specimens.Conclusions: Differential responses in mitochondrial dysfunction across cell lines highlight disease heterogeneity. MitoQ and Idb protected CEnCs from oxidative stress and improved mitochondrial bioenergetics, suggesting that mitochondrial-targeted antioxidants could be considered for mitochondrial dysfunction in CEnCs.","PeriodicalId":10710,"journal":{"name":"Cornea","volume":" ","pages":"492-503"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Letter Regarding: Validation of the C-DU(KE) Calculator as a Predictor of Outcomes in Patients Enrolled in Steroids for Corneal Ulcer and Mycotic Ulcer Treatment Trials. 关于：C-DU（KE）计算器作为角膜溃疡和真菌性溃疡类固醇治疗试验患者预后预测因子的验证

IF 1.9 3区医学

Cornea Pub Date : 2025-04-01 Epub Date: 2024-10-10 DOI: 10.1097/ICO.0000000000003733

Ricko Wong

引用次数: 0

Reply. 回复。

IF 1.9 3区医学

Cornea Pub Date : 2025-04-01 Epub Date: 2025-01-17 DOI: 10.1097/ICO.0000000000003794

Alejandro Arboleda, N Venkatesh Prajna, Muthiah Srinivasan, Hazem Mousa, Victor L Perez, Jennifer Rose-Nussbaumer

引用次数: 0

Novel Technique to Close Stellate Corneal Lacerations. 缝合星状角膜裂伤的新技术

IF 1.9 3区医学

Cornea Pub Date : 2025-04-01 Epub Date: 2024-10-29 DOI: 10.1097/ICO.0000000000003730

Steven E Brooks, Alex Johnson, Emily A Kreuger

{"title":"Novel Technique to Close Stellate Corneal Lacerations.","authors":"Steven E Brooks, Alex Johnson, Emily A Kreuger","doi":"10.1097/ICO.0000000000003730","DOIUrl":"10.1097/ICO.0000000000003730","url":null,"abstract":"Purpose: The study was performed to evaluate and optimize a novel technique to close stellate corneal lacerations in a wet laboratory environment.Materials and methods: Twenty-one ex vivo porcine eyes with standardized, experimentally created stellate corneal lacerations were assigned to one of three surgical repair techniques. In Group 1 (n = 9), lacerations were closed with simple interrupted sutures only. In Group 2 (n = 7), a large-radius central purse string was used in addition to simple interrupted sutures. In Group 3 (n = 5), a small-radius central purse string was used in addition to simple interrupted sutures. Wound stability was assessed by determining the intraocular pressure (IOP) at which wound leak occurred.Results: Group 3 eyes demonstrated a mean wound leak IOP of 56.1 mm Hg (range 32-90.3) compared with groups 1 and 2, which demonstrated mean wound leak IOPs of 6.4 mm Hg (range 1.9-19) and 13.3 mm Hg (range 2.8-31), respectively. These differences were highly statistically significant ( P < 0.002 and P < 0.015, respectively, compared with Group 3).Discussion: The use of a small-radius internal purse-string suture, when combined with simple interrupted sutures, produced a highly effective watertight closure in experimentally created stellate corneal lacerations, significantly outperforming approaches using only interrupted sutures or a large-radius purse string. The use of a small-radius purse-string suture should be considered for clinical use in the repair of stellate corneal lacerations.","PeriodicalId":10710,"journal":{"name":"Cornea","volume":" ","pages":"522-524"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Letter Regarding: Novel Mechanisms Guide Innovative Molecular-Based Therapeutic Strategies for Fuchs Endothelial Corneal Dystrophy. 关于ATF6背景下的Fuchs内皮性角膜营养不良的信函。

IF 1.9 3区医学

Cornea Pub Date : 2025-04-01 Epub Date: 2025-01-14 DOI: 10.1097/ICO.0000000000003809

Nektarios Barabutis

引用次数: 0

Analysis of Corneal Phenotypes in Japanese Patients With Myotonic Dystrophy Type 1. 日本 1 型肌营养不良症患者的角膜表型分析

IF 1.9 3区医学

Cornea Pub Date : 2025-04-01 Epub Date: 2024-08-21 DOI: 10.1097/ICO.0000000000003679

Kenya Kubo, Yoshinori Oie, Ryota Koto, Nozomi Nishida, Chifune Kai, Sayo Maeno, Tomoya Kubota, Masayuki Nakamori, Masanori P Takahashi, Motokazu Tsujikawa, Kohji Nishida

{"title":"Analysis of Corneal Phenotypes in Japanese Patients With Myotonic Dystrophy Type 1.","authors":"Kenya Kubo, Yoshinori Oie, Ryota Koto, Nozomi Nishida, Chifune Kai, Sayo Maeno, Tomoya Kubota, Masayuki Nakamori, Masanori P Takahashi, Motokazu Tsujikawa, Kohji Nishida","doi":"10.1097/ICO.0000000000003679","DOIUrl":"10.1097/ICO.0000000000003679","url":null,"abstract":"Purpose: To analyze the corneal phenotypes of Japanese patients with myotonic dystrophy type 1 (DM1).Methods: We included patients with DM1 who were diagnosed with clinical neuromuscular symptoms by neurologists and CTG trinucleotide repeat (TNR) expansion of the (myotonic dystrophy protein kinase) DMPK gene. We analyzed the corneal phenotype using slit-lamp examination, specular microscopy, and anterior segment optical coherence tomography. We evaluated TNR expansion in the TCF4 gene of leukocyte-derived genomic DNA by fragment analysis using polymerase chain reaction and triplet-repeat primed polymerase chain reaction.Results: Nineteen eyes from 10 patients with DM1 (DM1 group) and 72 eyes from 37 healthy participants (control group) were analyzed. The average age was 49.3 ± 11.9 and 51.8 ± 12.9 years in the DM1 and control groups, respectively ( P = 0.11). Slit-lamp examination demonstrated that 2 patients with DM1 had bilateral corneal guttae equivalent to modified Krachmer grade 1 of Fuchs endothelial corneal dystrophy. Dark areas on specular microscopy were observed in 4 of 19 eyes (21.1%) and 0 of 72 eyes (0%) in the DM1 and control groups, respectively, with statistically significant differences ( P = 0.002). The average endothelial cell density in the DM1 group (3536 ± 722 cells/mm 2 ) was significantly higher than that in the control group (3026 ± 412 cells/mm 2 ) ( P = 0.0006). TNR expansion in TCF4 was not detected in eyes with corneal guttae or in the dark areas in the DM1 group.Conclusions: Japanese patients with DM1 without TNR expansion in TCF4 have a mild phenotype equivalent to Fuchs endothelial corneal dystrophy. Endothelial cell density is higher in DM1 patients than in normal participants.","PeriodicalId":10710,"journal":{"name":"Cornea","volume":" ","pages":"412-417"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142016645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Automated and Manual Measurement of Corneal Epithelial Thickness in Eyes With Limbal Stem Cell Deficiency. 角膜缘干细胞缺乏症患者角膜上皮厚度的自动测量与手动测量比较

IF 1.9 3区医学

Cornea Pub Date : 2025-04-01 Epub Date: 2024-08-27 DOI: 10.1097/ICO.0000000000003682

Thanachaporn Kittipibul, Rutuja Unhale, Clemence Bonnet, Chi-Hong Tseng, Sophie X Deng

{"title":"Comparison of Automated and Manual Measurement of Corneal Epithelial Thickness in Eyes With Limbal Stem Cell Deficiency.","authors":"Thanachaporn Kittipibul, Rutuja Unhale, Clemence Bonnet, Chi-Hong Tseng, Sophie X Deng","doi":"10.1097/ICO.0000000000003682","DOIUrl":"10.1097/ICO.0000000000003682","url":null,"abstract":"Purpose: The purpose of this study was to compare the outcomes of manual and automated corneal epithelial thickness (CET) assessments using anterior segment optical coherence tomography in eyes with limbal stem cell deficiency (LSCD).Methods: Eighty-seven eyes from 64 patients with LSCD and 65 eyes from 39 healthy subjects were included in this cross-sectional study. OCT images of corneas were acquired using a Fourier-domain anterior segment optical coherence tomography. The automated measurement of CET was obtained using the manufacturer's program and the manual measurements were obtained by 2 masked observers. The average CET was obtained from 3 consecutive epithelial profile maps. The average CET of manual measurement was obtained from 3 consecutive cross-line scans using 3-point measurement as previously described.Results: The central CET was thinner in patients with LSCD compared with healthy individuals by both manual and automated measurements. Automated CET measurements were similar to the manually obtained CET in the healthy controls (r = 0.911). However, there is a lower correlation between the automated and manually obtained CET in the LSCD group (r = 0.497). Manual measurements of CET (39.8 ± 11 μm) in eyes with LSCD were significantly lower than the automated measurements (48.8 ± 7.2 μm, P = 0.001, 95% CI -11.0 to -6.9). The correlations between the automated and manually obtained measurements in the mild, moderate, and severe stages of LSCD were 0.623, 0.632, and 0.378, respectively.Conclusions: There is a large discrepancy between the manual and automated measurements in eyes with LSCD but not in normal eyes. Automated measurements in LSCD exhibited a higher rate of errors and nonuniform measurements due to the presence of subepithelial scars. Automated CET measurements appear to be unreliable in eyes with corneal scars in LSCD.","PeriodicalId":10710,"journal":{"name":"Cornea","volume":" ","pages":"422-426"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142085933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyanoacrylate Glue Patch for Corneal Melting in Keratoprosthesis. 氰基丙烯酸酯胶贴用于角膜修复术中角膜融化。

IF 1.9 3区医学

Cornea Pub Date : 2025-04-01 DOI: 10.1097/ICO.0000000000003875

Gustavo Ortiz-Morales, Guillermo Raul Vera-Duarte, Thelma Cortés-Moreno, Mariana Navarrete-Azuara, Panotsom Ngowyutagon, Alejandro Navas, Arturo Ramirez-Miranda, M Soledad Cortina, Enrique O Graue-Hernandez

{"title":"Cyanoacrylate Glue Patch for Corneal Melting in Keratoprosthesis.","authors":"Gustavo Ortiz-Morales, Guillermo Raul Vera-Duarte, Thelma Cortés-Moreno, Mariana Navarrete-Azuara, Panotsom Ngowyutagon, Alejandro Navas, Arturo Ramirez-Miranda, M Soledad Cortina, Enrique O Graue-Hernandez","doi":"10.1097/ICO.0000000000003875","DOIUrl":"https://doi.org/10.1097/ICO.0000000000003875","url":null,"abstract":"Purpose: To report clinical outcomes of cyanoacrylate glue patches in managing corneal melting in keratoprosthesis (KPro).Methods: Multicenter, retrospective, noncontrolled, interventional case series. Subjects underwent cyanoacrylate glue patches for corneal melt after KPro implantation, regardless of the underlying cause, KPro design, or melt severity. Clinical success was defined as resolving aqueous leak, halting further corneal melting, and avoiding KPro or carrier corneal graft exchange for at least 12 months after cyanoacrylate glue patch application.Results: Sixteen eyes of 15 patients with KPro underwent a cyanoacrylate glue patch for corneal melting. The mean time from KPro implantation to the development of corneal melt was 49.2 months. Implanted KPro models included Lucia (37.5%) and Boston type 1 with titanium (31.2%) or polymethylacrylate backplate (31.2%). Underlying etiologies for KPro implantation included recurrent graft rejection (56.2%), autoimmune disease (31.2%), and chemical injury (12.5%). A combined procedure with a cyanoacrylate glue patch and amniotic membrane transplantation was performed in 2 cases. The criteria for clinical success were met in 87.5% of patients, with only 2 eyes (12.5%) exhibiting clinical failure. Glue patches were retained successfully for a mean time of 25.50 ± 23.87 months (range: 0-84 months).Conclusions: Cyanoacrylate glue patches provide a simple, safe, cost-effective, and effective treatment for corneal melts in patients with KPro.","PeriodicalId":10710,"journal":{"name":"Cornea","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143763251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of the Direct Anterior Chamber Air Replacement Method During Descemet Stripping Automated Endothelial Keratoplasty. 直接前房空气置换法在角膜内皮剥离自动角膜移植术中的疗效。

IF 1.9 3区医学

Cornea Pub Date : 2025-04-01 Epub Date: 2025-01-17 DOI: 10.1097/ICO.0000000000003784

Sho Ishikawa, Takafumi Maruyama, Kei Shinoda

{"title":"Efficacy of the Direct Anterior Chamber Air Replacement Method During Descemet Stripping Automated Endothelial Keratoplasty.","authors":"Sho Ishikawa, Takafumi Maruyama, Kei Shinoda","doi":"10.1097/ICO.0000000000003784","DOIUrl":"10.1097/ICO.0000000000003784","url":null,"abstract":"Purpose: This study aimed to describe a novel technique of direct anterior chamber (AC) air replacement (DACAR) for the management of Descemet stripping automated endothelial keratoplasty (DSAEK) in postvitrectomized eyes and eyes with previous glaucoma surgery.Methods: DACAR was performed after a corneal donor graft was transplanted through a wound using the pull-through technique. DACAR involves stabilizing the graft with forceps while introducing air into the AC via an infusion cannula to ensure complete air exchange. The air was maintained in the AC at all times using a vitrectomy machine. The air pressure was maintained at 30 mm Hg for 15 minutes.Results: The DACAR technique was performed in 34 patients, and conventional pull-through technique DSAEK was performed in 32 high-risk patients. The DACAR group had shorter DSAEK surgical procedures ( P = 0.009) and a lower incidence of corneal graft detachment in the early postoperative period ( P < 0.001) than the conventional DSAEK group.Conclusions: DACAR is performed in patients having previously undergone vitrectomy or glaucoma surgery to prevent corneal graft detachment during the early postoperative period and to reduce the length of surgery.","PeriodicalId":10710,"journal":{"name":"Cornea","volume":" ","pages":"517-521"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11872274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Central Cornea Changes on Anterior Segment OCT and In Vivo Confocal Microscopy After Autologous Limbal Epithelial Cell Transplantation. 自体角膜缘上皮细胞移植后中央角膜前段OCT和体内共聚焦显微镜的变化。

IF 1.9 3区医学

Cornea Pub Date : 2025-03-28 DOI: 10.1097/ICO.0000000000003865

Leyla Yavuz Saricay, Aaron R Kaufman, Lynette K Johns, Jia Yin, Lassana Samarakoon, Allison R Ayala, Maureen Maguire, Mohit Parekh, Diego E Hernandez Rodriguez, Heather Daley, Reza Dana, Myriam Armant, Jerome Ritz, Ula V Jurkunas

{"title":"Central Cornea Changes on Anterior Segment OCT and In Vivo Confocal Microscopy After Autologous Limbal Epithelial Cell Transplantation.","authors":"Leyla Yavuz Saricay, Aaron R Kaufman, Lynette K Johns, Jia Yin, Lassana Samarakoon, Allison R Ayala, Maureen Maguire, Mohit Parekh, Diego E Hernandez Rodriguez, Heather Daley, Reza Dana, Myriam Armant, Jerome Ritz, Ula V Jurkunas","doi":"10.1097/ICO.0000000000003865","DOIUrl":"https://doi.org/10.1097/ICO.0000000000003865","url":null,"abstract":"Purpose: To describe 1-year changes in the cornea as assessed by anterior segment optical coherence tomography (AS-OCT) and in vivo confocal microscopy (IVCM) for participants receiving a tissue graft generated from a new manufacturing process using cultivated autologous limbal epithelial cells.Methods: Cultivated autologous limbal epithelial cell grafts were produced in a 2-stage manufacturing process following a good manufacturing process-compliant protocol. AS-OCT and IVCM were completed at baseline and 12 months after the treatment in subsets of these participants. Secondary efficacy outcomes were determined based on improvement of central corneal epithelial morphology and thickness [corneal epithelial thickness (CET)] and presence of conjunctival or corneal cells in central cornea.Results: Among 14 participants, 13 (93%) were male, 12 (86%) were white, the mean age was 46 ± 16 years. At baseline, CET was 53 (range: 34, 64) microns, and epithelial basal cell density was 3964 (range: 822-5788) cells/mm2; the ratio of the cells at central cornea was 20% corneal and 90% conjunctival epithelial cells. At 12 months, the mean changes were 3 μm in CET (P = 0.67), and 1967 cells/mm2 in epithelial basal cell density (P = 0.02); the proportion of the central cells improved to 75% corneal and 38% conjunctival epithelial cells.Conclusions: The AS-OCT and IVCM findings are consistent with the clinical improvement, indicating the reconstitution of the corneal phenotype and clearing of the optical axis. Nevertheless, IVCM is notably more effective for in-depth analysis of the epithelial phenotype and thickness than AS-OCT.","PeriodicalId":10710,"journal":{"name":"Cornea","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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