Conservation Genetics Resources最新文献_第2页

Dietary analysis of wolf (Canis lupus) – a comparison of markers and methods 狼（Canis lupus）的膳食分析--标记物和方法的比较

IF 1.1 4区环境科学与生态学

Conservation Genetics Resources Pub Date : 2024-04-30 DOI: 10.1007/s12686-024-01356-4

Pascal Eusemann, Jana Rees, Vivian Kuhlenkamp, Paul Lippitsch, Heiner Schumann

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Identification and characterization of 29 microsatellite loci for Tripneustes gratilla based on high-throughput sequencing 基于高通量测序鉴定和表征三棘鲷的 29 个微卫星位点

IF 1.1 4区环境科学与生态学

Conservation Genetics Resources Pub Date : 2024-04-30 DOI: 10.1007/s12686-024-01355-5

Chenhu Yang, Ruoqin Sun, Na Du, Xiaoqi Zeng, Gang Ni

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Technical note: successful DNA amplification of DNA from non-destructive buccal swabbing in Vespertilionid and Rhinolophid bats 技术说明：从非破坏性颊拭子中成功扩增蝙蝠和鼻蝠科蝙蝠的 DNA

IF 1.1 4区环境科学与生态学

Conservation Genetics Resources Pub Date : 2024-03-22 DOI: 10.1007/s12686-024-01352-8

Morgan Hughes, Scott K Brown, Rémi Martin, Christopher H Young, Simon Maddock

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Development of polymorphic microsatellite markers for genetic stock identification of green chromide, Etroplus suratensis using next generation sequencing technology 利用下一代测序技术开发多态性微卫星标记，用于识别绿染色蝶（Etroplus suratensis）的遗传种群

IF 1.1 4区环境科学与生态学

Conservation Genetics Resources Pub Date : 2024-03-22 DOI: 10.1007/s12686-024-01350-w

Magdeline Christo, Divya Merin Jose, P. R. Divya, M. U. Rekha, U. K. Sarkar

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Isolation and characterization of 35 SNP markers of Leiobagrus kingi (Teleostei, Siluriformes: Amblycipitidae) 雷鱼（Teleostei, Siluriformes: Amblycipitidae）35个SNP标记的分离和特征描述

IF 1.1 4区环境科学与生态学

Conservation Genetics Resources Pub Date : 2024-03-22 DOI: 10.1007/s12686-024-01349-3

Wei Li, Xiang-Yang Jia

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Development of a set of SNP markers for population genetics of the sea rose, Pentapora fascialis 为海玫瑰（Pentapora fascialis）的种群遗传学开发一套 SNP 标记

IF 1.1 4区环境科学与生态学

Conservation Genetics Resources Pub Date : 2024-02-17 DOI: 10.1007/s12686-024-01348-4

M Padrón, M. Milhes, M. Massot, E. Guichoux, Guizien K.

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An improved genetic marker panel for conservation monitoring of upper Kootenay River burbot 用于监测库特奈河上游水豚保护情况的改良遗传标记组

IF 1.1 4区环境科学与生态学

Conservation Genetics Resources Pub Date : 2024-02-14 DOI: 10.1007/s12686-024-01351-9

Audrey C. Harris, D. Katharine Coykendall, Heather Lamson, Ryan P. Kovach, James L. Dunnigan, Matthew R. Campbell

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A IISNPs panel for the giant panda (Ailuropoda melanoleuca) 大熊猫（Ailuropoda melanoleuca）IISNPs 小组

IF 1.1 4区环境科学与生态学

Conservation Genetics Resources Pub Date : 2024-02-14 DOI: 10.1007/s12686-024-01347-5

Fujun Shen, Kaile Ning, Wei Xu, Yan Li, Jiawen Liu, Hong Liu, Juan Wang, Liang Zhang, Li Luo, Kongju Wu

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Improved amplification of fecal DNA supports non-invasive microsatellite genotyping of lesser long-nosed bats (Leptonycteris yerbabuenae) 改进粪便 DNA 扩增技术，支持对小长鼻蝙蝠（Leptonycteris yerbabuenae）进行非侵入式微卫星基因分型

IF 1.1 4区环境科学与生态学

Conservation Genetics Resources Pub Date : 2024-02-04 DOI: 10.1007/s12686-023-01344-0

John-Lee Sky Walker, Robert J. Steidl, Sandy A. Wolf, Ming-Min Lee, A. Elizabeth Arnold

{"title":"Improved amplification of fecal DNA supports non-invasive microsatellite genotyping of lesser long-nosed bats (Leptonycteris yerbabuenae)","authors":"John-Lee Sky Walker, Robert J. Steidl, Sandy A. Wolf, Ming-Min Lee, A. Elizabeth Arnold","doi":"10.1007/s12686-023-01344-0","DOIUrl":"https://doi.org/10.1007/s12686-023-01344-0","url":null,"abstract":"Feces of animals that forage on nectar and fruit, including many species of bats, often contain DNA that is low in quality and quantity. We developed an approach based on DNA from feces gathered passively to generate microsatellite data for individual lesser long-nosed bats (Leptonycteris yerbabuenae), which are important pollinators for columnar cacti and agave across much of Mexico and in the southwestern U.S. We collected feces from roosts near the U.S-Mexico border and developed a two-step amplification approach to characterize five highly polymorphic microsatellite loci from fecal DNA. Addition of a multiplex PCR step improved amplification success and conserved DNA extracts with a minimal increase in cost. In our initial screening of 433 samples, five focal loci distinguished individuals reliably, with a probability of identity (i.e., the probability of two unrelated individuals having the same microsatellite profile by chance) of 7.5E-09. Repeated analyses revealed a genotyping error rate < 2%. We explore the benefits and limits of our approach for population studies of lesser long-nosed bats and other nectivorous and frugivorous species that provide key ecosystem services and are often of conservation concern.","PeriodicalId":10625,"journal":{"name":"Conservation Genetics Resources","volume":"18 1","pages":""},"PeriodicalIF":1.1,"publicationDate":"2024-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139678035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development, verification, and implementation of an eDNA detection assay for emydids with a case study on diamondback terrapins, Malaclemys terrapin, and red eared slider, Trachemys script elegans 开发、验证和实施针对贻贝的 eDNA 检测分析法，并对菱背陆龟（Malaclemys terrapin）和红耳舄（Trachemys script elegans）进行案例研究

IF 1.1 4区环境科学与生态学

Conservation Genetics Resources Pub Date : 2024-02-03 DOI: 10.1007/s12686-023-01343-1

Andrew T. Fields, Elizabeth P. Hunt, Katherine Lanoue, Aaron S. Baxter, Riley S. Sebby, W. Jay Tarkington, David S. Portnoy

{"title":"Development, verification, and implementation of an eDNA detection assay for emydids with a case study on diamondback terrapins, Malaclemys terrapin, and red eared slider, Trachemys script elegans","authors":"Andrew T. Fields, Elizabeth P. Hunt, Katherine Lanoue, Aaron S. Baxter, Riley S. Sebby, W. Jay Tarkington, David S. Portnoy","doi":"10.1007/s12686-023-01343-1","DOIUrl":"https://doi.org/10.1007/s12686-023-01343-1","url":null,"abstract":"Globally, many pond turtles (Family Emydidae) are of conservation concern due to contracting ranges, increasingly fragmented habitats, and declining populations. One of these turtles, the diamondback terrapin (Malaclemys terrapin), is of concern within the United States, but surveying potential estuarine habitat for the presence of the species requires high levels of time and effort. Here a species-specific, probe-based qPCR assay for identifying terrapin DNA from environmental DNA (eDNA) is described. To expand the utility of this tool, the assay was designed to allow for potential detection of at least 19 other pond turtles taxa and the utility was tested by designing a second species-specific probe for red-eared slider (Trachemys scripta elegans) with a different fluorophore, which can be used simultaneously with the terrapin probe to detect either species. Probes were found to be species-specific and effective at detecting low levels of DNA for the target species, the diamondback terrapin (10 fg/µL) and the red-eared slider (100 fg/µL) in laboratory conditions. The assay also showed species-specific detection in environmental samples taken from field locations where one species or the other were known to reside. Through citizen science approaches, this assay could be used for discovering novel terrapin habitats, monitoring invasive red-eared slider populations or surveying other emydids, with species-specific probes, at a fraction of the cost of traditional surveys.","PeriodicalId":10625,"journal":{"name":"Conservation Genetics Resources","volume":"10 1","pages":""},"PeriodicalIF":1.1,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139678034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0