Morgan Hughes, Scott K Brown, Rémi Martin, Christopher H Young, Simon Maddock
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引用次数: 0
Abstract
Acquiring DNA from wild bats (Mammalia: Chiroptera) is typically undertaken utilizing highly invasive (but non-lethal) sampling techniques comprising wing biopsies and occasional blood samples. While non-invasive sampling is possible through the extraction of DNA from faecal samples, it is not always possible to acquire samples from individual bats whilst conducting fieldwork, and as such, this method is primarily applicable to roost occupancy identification. Similarly, wing swabbing is liable to cross-contamination from roost mates. Here we present the first use of oral (buccal) swabbing for successful, species-resolution DNA sequencing of Vespertilionidae and Rhinolophidae in 10 bat species (nine Vespertilionidae and one Rhinolophidae) from the UK.
从野生蝙蝠（哺乳纲：爬行纲）身上获取 DNA 通常采用高度侵入性（但非致命性）的取样技术，包括翅膀活检和偶尔的血液样本。虽然可以通过从粪便样本中提取 DNA 来进行非侵入性取样，但在进行野外工作时并不总能从单个蝙蝠身上获取样本，因此这种方法主要适用于巢穴栖息地的鉴定。同样，拭取翅膀样本也容易受到栖息地同伴的交叉感染。在这里，我们首次利用口腔（颊）拭子成功地对英国的 10 种蝙蝠（9 种蝙蝠科和 1 种鼻蝠科）进行了物种分辨率 DNA 测序。
期刊介绍：
Conservation Genetics Resources promotes the conservation of genetic diversity and advances the study of conservation genetics by providing rapid publication of technical papers and reviews on methodological innovations or improvements, computer programs, and genomic resources, as well as on the practical application of these resources towards the development of effective conservation policy and practice.
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