Divya Merin Jose, Divya Prema Radhakrishnan, Kuldeep Kumar Lal
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Development of novel microsatellite markers for aquaculture important species, milkfish (Chanos chanos Forsskål, 1775) with implications of conservation and management
A total of 20 polymorphic microsatellites were developed and characterized for a potential aquaculture species, Chanos chanos, utilizing the comprehensive whole genome data of C. chanos obtained from the GenBank database (accession number: LR697106). Using the MIcroSAtellite (MISA) tool, potential microsatellite motifs were identified in the assembled contigs and unigenes and with the PRIMER 3 input software (Version 4.1.0), new primers were developed. The number of alleles per locus in C. chanos varied from 9 to 16, with observed heterozygosities ranging from 0.710 to 0.926, while the expected heterozygosities were within the range of 0.720 to 0.902. Among the 20 microsatellite loci, the majority exhibited PIC values > 0.75. Validation of these microsatellite markers proved that these markers can be used as a significant tool for analysing genetic differentiation as well genetic relationships among C. chanos.
期刊介绍：
Conservation Genetics Resources promotes the conservation of genetic diversity and advances the study of conservation genetics by providing rapid publication of technical papers and reviews on methodological innovations or improvements, computer programs, and genomic resources, as well as on the practical application of these resources towards the development of effective conservation policy and practice.
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