Communications Biology最新文献_第10页

The tumor microenvironment of metastatic osteosarcoma in the human and canine lung. 人与犬肺转移性骨肉瘤的肿瘤微环境。

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-05-15 DOI: 10.1038/s42003-025-07992-2

L E McGee, J S Pereira, T A McEachron, C Mazcko, A K LeBlanc, J A Beck

引用次数: 0

Cas3 of type I-Fa CRISPR-Cas system upregulates bacterial biofilm formation and virulence in Acinetobacter baumannii. I-Fa型CRISPR-Cas系统中的Cas3上调鲍曼不动杆菌的细菌生物膜形成和毒力。

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-05-14 DOI: 10.1038/s42003-025-08124-6

Tingting Guo, Jie Yang, Na Zhou, Xiaoli Sun, Changchao Huan, Tao Lin, Guangyu Bao, Jian Hu, Guocai Li

{"title":"Cas3 of type I-Fa CRISPR-Cas system upregulates bacterial biofilm formation and virulence in Acinetobacter baumannii.","authors":"Tingting Guo, Jie Yang, Na Zhou, Xiaoli Sun, Changchao Huan, Tao Lin, Guangyu Bao, Jian Hu, Guocai Li","doi":"10.1038/s42003-025-08124-6","DOIUrl":"https://doi.org/10.1038/s42003-025-08124-6","url":null,"abstract":"Acinetobacter baumannii (A. baumannii) is an important pathogen causing various nosocomial infections. CRISPR-Cas system is the adaptive immune system of bacteria, which is also closely related to the drug resistance and virulence of bacteria. However, the effect and mechanism of cas3 (type I-Fa) in A. baumannii is still unclear. In this study, we successfully constructed a cas3 deletion mutant (19606Δcas3) and complemented strain (19606Δcas3/pcas3) to study the regulatory mechanism of type I-Fa cas3 on bacterial virulence. Our results showed that deletion of cas3(type I-Fa) significantly reduced the biofilm formation, virulence and pathogenicity to mice. The organ bacterial load of mice infected with cas3 deletion strain was significantly reduced, the lung inflammation was slightly changed, and the serum cytokine level was also decreased. All results demonstrated that cas3 enhanced the virulence and pathogenicity of A. baumannii. Mechanism analysis showed that deletion of cas3 can lead to the down-regulation of virulence factors such as biofilm formation related factors and outer membrane protein A(ompA). In addition, cas3 was also involved in the regulation of carbon metabolism and oxidative phosphorylation pathway of A. baumannii. Altogether, our study may provide cas3 as a therapeutic target in the future because of the close link to the virulence of A. baumannii.","PeriodicalId":10552,"journal":{"name":"Communications Biology","volume":"8 1","pages":"750"},"PeriodicalIF":5.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ATF1 and miR-27b-3p drive intervertebral disc degeneration through the PPARG/NF-κB signaling axis. ATF1和miR-27b-3p通过PPARG/NF-κB信号轴驱动椎间盘退变。

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-05-14 DOI: 10.1038/s42003-025-08186-6

Wei Guo, Kun Mu, Jing-Chao Geng, Hai-Yang Xing, Yu Dong, Wen-Dong Liu, Shuan-Chi Wang, Jia-Xiao Shi, Bao-Rui Xing, Jian-Yong Zhao, Xiao-Ming Li

{"title":"ATF1 and miR-27b-3p drive intervertebral disc degeneration through the PPARG/NF-κB signaling axis.","authors":"Wei Guo, Kun Mu, Jing-Chao Geng, Hai-Yang Xing, Yu Dong, Wen-Dong Liu, Shuan-Chi Wang, Jia-Xiao Shi, Bao-Rui Xing, Jian-Yong Zhao, Xiao-Ming Li","doi":"10.1038/s42003-025-08186-6","DOIUrl":"https://doi.org/10.1038/s42003-025-08186-6","url":null,"abstract":"Intervertebral disc degeneration (IDD) is a primary cause of degenerative disc disease; however, the mechanisms underlying it remain unknown. Although great efforts have been made to develop new regenerative therapies, their clinical success is limited. Recent research has indicated that microRNAs (miRNAs) are significantly involved in the progression of IDD. Investigating the role of miRNA intervention in IDD could facilitate the development of therapeutic strategies based on miRNAs. However, circulating miRNAs have not yet been recognized as standard biomarkers for IDD. In this study, we observed that the expression of miR-27b-3p was elevated in the blood and nucleus pulposus (NP) tissue of patients with IDD. Furthermore, reducing the expression of miR-27b-3p was shown to impede the progression of IDD. MiR-27b-3p could reduce the expression of collagen II and ACAN and promote the expression of MMP13 and ADAMT-5 in vitro and in vivo. miR-27b-3p aggravated IDD progression by directly targeting peroxisome proliferator-activated receptor gamma (PPARG), a negative regulator of the NF-κB signal pathway. This study also established that PPARG serves a protective role in IDD. The overexpression of PPARG was able to mitigate the detrimental effects caused by miR-27b-3p in NP cells and animal models of IDD, indicating that miR-27b-3p facilitates the progression of IDD through its interaction with PPARG. Additionally, the transcription factor ATF1 was found to enhance the expression of miR-27b-3p by targeting its promoter region, thereby promoting the degenerative impact of miR-27b-3p on NP cells. Given that miR-27b-3p can promote IDD both in vitro and in vivo, it holds potential as a biomarker, and the inhibition of miR-27b-3p expression may represent a novel therapeutic target for IDD.","PeriodicalId":10552,"journal":{"name":"Communications Biology","volume":"8 1","pages":"751"},"PeriodicalIF":5.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078598/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abstract choice representations during stable choice-response associations. 稳定选择-反应关联中的抽象选择表征。

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-05-14 DOI: 10.1038/s42003-025-08129-1

Katrina R Quinn, Florian Sandhaeger, Nima Noury, Ema Zezelic, Markus Siegel

{"title":"Abstract choice representations during stable choice-response associations.","authors":"Katrina R Quinn, Florian Sandhaeger, Nima Noury, Ema Zezelic, Markus Siegel","doi":"10.1038/s42003-025-08129-1","DOIUrl":"https://doi.org/10.1038/s42003-025-08129-1","url":null,"abstract":"An increasing body of evidence has demonstrated neural representations of choices independent of the motor actions used to report them - so-called abstract choices. However, it remains unclear whether such representations arise due to dynamic changes in choice-response associations or reflect a general property of decision-making. Here, we show that in the human brain, choices are represented abstractly even when choice-response associations remain stable over time. We recorded neural activity using magnetoencephalography while participants performed a motion discrimination task, with choice-response mappings held constant within blocks. We found neural information about participants' perceptual choices independent of both motor response and visual stimulus. Choice information increased during the stimulus and peaked after the response. Moreover, choice and response information showed distinct cortical distributions, with choice-related signals strongest in frontoparietal regions. Thus, abstract choice representations are not limited to dynamic or action-independent contexts and may be a general feature of decision-making.","PeriodicalId":10552,"journal":{"name":"Communications Biology","volume":"8 1","pages":"752"},"PeriodicalIF":5.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systematic transcriptomics analysis of calorie restriction and rapamycin unveils their synergistic interaction in prolonging cellular lifespan. 热量限制和雷帕霉素的系统转录组学分析揭示了它们在延长细胞寿命方面的协同相互作用。

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-05-14 DOI: 10.1038/s42003-025-08178-6

Yizhong Zhang, Arshia Naaz, Trishia Yi Ning Cheng, Jovian Jing Lin, Mingtong Gao, Rajkumar Dorajoo, Mohammad Alfatah

{"title":"Systematic transcriptomics analysis of calorie restriction and rapamycin unveils their synergistic interaction in prolonging cellular lifespan.","authors":"Yizhong Zhang, Arshia Naaz, Trishia Yi Ning Cheng, Jovian Jing Lin, Mingtong Gao, Rajkumar Dorajoo, Mohammad Alfatah","doi":"10.1038/s42003-025-08178-6","DOIUrl":"https://doi.org/10.1038/s42003-025-08178-6","url":null,"abstract":"Aging is a multifaceted biological process marked by the decline in both mitotic and postmitotic cellular function, often central to the development of age-related diseases. In the pursuit of slowing or even reversing the aging process, a prominent strategy of significant interest is calorie restriction (CR), also known as dietary restriction, and the potential influence of a drug called rapamycin (RM). Both CR and RM have demonstrated the capacity to extend healthspan and lifespan across a diverse array of species, including yeast, worms, flies, and mice. Nevertheless, their individual and combined effects on mitotic and postmitotic cells, as well as their comparative analysis, remain areas that demand a thorough investigation. In this study, we employ RNA-sequencing methodologies to comprehensively analyze the impact of CR, RM, and their combination (CR + RM) on gene expression in yeast cells. Our analysis uncovers distinctive, overlapping, and even contrasting patterns of gene regulation, illuminating the unique and shared effects of CR and RM. Furthermore, the transcriptional synergistic interaction of CR + RM is validated in extending the lifespan of both yeast and human cells.","PeriodicalId":10552,"journal":{"name":"Communications Biology","volume":"8 1","pages":"753"},"PeriodicalIF":5.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078523/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zebrafishology, study design guidelines for rigorous and reproducible data using zebrafish. 斑马鱼学，研究设计指南严格和可重复的数据使用斑马鱼。

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-05-13 DOI: 10.1038/s42003-025-07496-z

Victoria M Bedell, Priya Dubey, Han B Lee, Dondra S Bailey, Jennifer L Anderson, Allison Jamieson-Lucy, Rui Xiao, Elvin V Leonard, Marni J Falk, Michael A Pack, Mary Mullins, Steven A Farber, Roderic G Eckenhoff, Stephen C Ekker

引用次数: 0

Identification of druggable binding sites and small molecules as modulators of TMC1. TMC1可药物结合位点和小分子调节剂的鉴定。

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-05-13 DOI: 10.1038/s42003-025-07943-x

Pedro De-la-Torre, Claudia Martínez-García, Paul Gratias, Matthew Mun, Paula Santana, Nurunisa Akyuz, Wendy González, Artur A Indzhykulian, David Ramírez

{"title":"Identification of druggable binding sites and small molecules as modulators of TMC1.","authors":"Pedro De-la-Torre, Claudia Martínez-García, Paul Gratias, Matthew Mun, Paula Santana, Nurunisa Akyuz, Wendy González, Artur A Indzhykulian, David Ramírez","doi":"10.1038/s42003-025-07943-x","DOIUrl":"10.1038/s42003-025-07943-x","url":null,"abstract":"Our ability to hear and maintain balance relies on the proper functioning of inner ear sensory hair cells, which translate mechanical stimuli into electrical signals via mechano-electrical transducer (MET) channels, composed of TMC1/2 proteins. However, the therapeutic use of ototoxic drugs, such as aminoglycosides and cisplatin, which can enter hair cells through MET channels, often leads to profound auditory and vestibular dysfunction. To date, our understanding of how small-molecule modulators interact with TMCs remains limited, hampering the discovery of novel drugs. Here, we propose a structure-based drug screening approach, integrating 3D-pharmacophore modeling, molecular dynamics simulations of the TMC1 + CIB2 + TMIE complex, and experimental validation. Our pipeline successfully identified three potential drug-binding sites within the TMC1 pore, phospholipids, and key amino acids involved in the binding of several compounds, as well as FDA-approved drugs that reduced dye uptake in cultured cochlear explants. Our pipeline offers a broad application for discovering modulators for mechanosensitive ion channels.","PeriodicalId":10552,"journal":{"name":"Communications Biology","volume":"8 1","pages":"742"},"PeriodicalIF":5.2,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075566/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic landscape and evolution of Acinetobacter pittii, an underestimated emerging nosocomial pathogen. 皮氏不动杆菌是一种被低估的新出现的医院病原体。

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-05-13 DOI: 10.1038/s42003-025-08156-y

Shengke Wang, Yan Zhou, Yuezhuo Wang, Keshu Tang, Danqi Wang, Jiawen Hong, Pengcheng Wang, Sheng Ye, Jie Yan, Shengkai Li, Zhemin Zhou, Jimei Du

{"title":"Genetic landscape and evolution of Acinetobacter pittii, an underestimated emerging nosocomial pathogen.","authors":"Shengke Wang, Yan Zhou, Yuezhuo Wang, Keshu Tang, Danqi Wang, Jiawen Hong, Pengcheng Wang, Sheng Ye, Jie Yan, Shengkai Li, Zhemin Zhou, Jimei Du","doi":"10.1038/s42003-025-08156-y","DOIUrl":"10.1038/s42003-025-08156-y","url":null,"abstract":"As a member of Acinetobacter calcoaceticus-baumannii complex, Acinetobacter pittii has been an emerging concern in nosocomial infection due to its increasing prevalence and multidrug resistance (MDR). However, its population structure remains broadly unknown, hampering efficient tracing of its transmission and evolution. In this study, we developed a distributed core genome multilocus sequence typing (dcgMLST) for A. pittii based on 750 genomes and employed it to map the genetic landscape and evolution of A. pittii. The results demonstrated that two hierarchical clustering (HC) levels effectively correspond to genetic diversity from species (HC1100) to natural populations (HC450), as well as that a predominant lineage, HC1100_4, accounts for 33.9% of A. pittii strains. Subsequent analysis revealed that specific gene gain and loss events within HC1100_4 are linked to adaptations to environmental stress. Moreover, we identified a cluster of multidrug-resistant plasmids PT_712 responsible for the dissemination of blaNDM-1 genes within the genus of Acinetobacter. This study provides a framework for characterizing genetic diversity, evolutionary dynamics, molecular population distribution, and tracing of A. pittii, which has the potential to improve infection control strategies and public health policy.","PeriodicalId":10552,"journal":{"name":"Communications Biology","volume":"8 1","pages":"738"},"PeriodicalIF":5.2,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inspiring scientific wonder, curiosity and critical thinking in young minds: an interview with Audrey Dussutour. 激发年轻人的科学奇迹，好奇心和批判性思维：对奥黛丽·杜苏图尔的采访。

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-05-13 DOI: 10.1038/s42003-025-08166-w

引用次数: 0

Gasdermin-D pores induce an inactivating caspase-4 cleavage that limits IL-18 production in the intestinal epithelium. Gasdermin-D气孔诱导灭活caspase-4裂解，限制肠上皮中IL-18的产生。

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-05-13 DOI: 10.1038/s42003-025-08183-9

J K Bruce, L Y Li, Y Tang, E Forster, N J Winsor, P Y Bi, C Krustev, S Keely, J E Lee, J R Rohde, H Y Gaisano, D J Philpott, S E Girardin

{"title":"Gasdermin-D pores induce an inactivating caspase-4 cleavage that limits IL-18 production in the intestinal epithelium.","authors":"J K Bruce, L Y Li, Y Tang, E Forster, N J Winsor, P Y Bi, C Krustev, S Keely, J E Lee, J R Rohde, H Y Gaisano, D J Philpott, S E Girardin","doi":"10.1038/s42003-025-08183-9","DOIUrl":"10.1038/s42003-025-08183-9","url":null,"abstract":"Intestinal epithelial-derived IL-18 is critical for homeostatic intestinal barrier function and is secreted through Gasdermin D (GSDMD) pores. Inflammasome activation is a prerequisite for both IL-18 maturation and GSDMD pore formation. However, GSDMD pores also cause pyroptotic cell death, which could be detrimental to the intestinal epithelial barrier. How epithelial cells balance the need to secrete IL-18 and to maintain barrier integrity remains poorly understood. In human intestinal epithelial cell lines and in primary human epithelial intestinal organoids, but not in immune cells, GSDMD plasma membrane pore formation by LPS electroporation and by gram-negative bacterial infection induced a non-conventional p37 caspase-4 fragment that was associated with reduced levels of mature IL-18. By contrast, limiting GSDMD plasma membrane pores pharmacologically and via point-mutagenesis prevented caspase-4 cleavage and increased IL-18 production, suggesting that p37 caspase-4 cleavage may regulate IL-18 maturation in the intestinal epithelium. In support, co-expression of caspase-4 cleavage mutants and IL-18 in HEK293T cells revealed that non-cleavable caspase-4 produced more mature IL-18 than cleaved caspase-4. Overall, these studies suggest that epithelial inflammasomes encode feedback pathways that control the balance between cytokine secretion and cell death. This may be an important mechanism to ensure homeostatic IL-18 production in the intestinal epithelium.","PeriodicalId":10552,"journal":{"name":"Communications Biology","volume":"8 1","pages":"737"},"PeriodicalIF":5.2,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12069520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0