Communications Biology最新文献

Dynamics of agonist-evoked opioid receptor activation revealed by FRET- and BRET-based opioid receptor conformation sensors.

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-02-08 DOI: 10.1038/s42003-025-07630-x

Sina B Kirchhofer, Claudia Kurz, Lorenz Geier, Anna-Lena Krett, Cornelius Krasel, Moritz Bünemann

引用次数: 0

Priestia megaterium cells are primed for surviving lethal doses of antibiotics and chemical stress.

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-02-08 DOI: 10.1038/s42003-025-07639-2

Manisha Guha, Abhyudai Singh, Nicholas C Butzin

引用次数: 0

Single cell transcriptome profiling reveals pathogenesis of bullous pemphigoid.

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-02-08 DOI: 10.1038/s42003-025-07629-4

Guirong Liang, Chenjing Zhao, Qin Wei, Suying Feng, Yetao Wang

{"title":"Single cell transcriptome profiling reveals pathogenesis of bullous pemphigoid.","authors":"Guirong Liang, Chenjing Zhao, Qin Wei, Suying Feng, Yetao Wang","doi":"10.1038/s42003-025-07629-4","DOIUrl":"https://doi.org/10.1038/s42003-025-07629-4","url":null,"abstract":"Bullous pemphigoid (BP) triggers profound functional changes in both immune and non-immune cells in the skin and circulation, though the underlying mechanisms remain unclear. In this study, we conduct single-cell transcriptome analysis of lesional and non-lesional skin, as well as blood samples from BP patients. In lesional skin, non-immune cells upregulate pathways related to metabolism, wound healing, immune activation, and cell migration. LAMP3+DCs from cDC2 show stronger pro-inflammatory signatures than those from cDC1, and VEGFA+ mast cells, crucial for BP progression, are predominantly in lesional skin. As BP patients transition from active to remission stages, blood B cell function shifts from differentiation and memory formation to increased type 1 interferon signaling and reduced IL-4 response. Blood CX3CR1+ ZNF683+ and LAG3+ exhausted T cells exhibit the highest TCR expansion among clones shared with skin CD8+T cells, suggesting their role in fueling skin CD8+T cell clonal expansion. Clinical BP severity correlates positively with blood NK cell IFN-γ production and negatively with amphiregulin (AREG) production. NK cell-derived AREG mitigates IFN-γ-induced keratinocyte apoptosis, suggesting a crucial balance between AREG and IFN-γ in BP progression. These findings highlight functional shifts in BP pathology and suggest potential therapeutic targets.","PeriodicalId":10552,"journal":{"name":"Communications Biology","volume":"8 1","pages":"203"},"PeriodicalIF":5.2,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unravelling the interplay of nitrogen nutrition and the Botrytis cinerea pectin lyase BcPNL1 in modulating Arabidopsis thaliana susceptibility.

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-02-08 DOI: 10.1038/s42003-025-07642-7

Antoine Davière, Aline Voxeur, Sylvie Jolivet, Luka Lelas, Samantha Vernhettes, Marie-Christine Soulié, Mathilde Fagard

{"title":"Unravelling the interplay of nitrogen nutrition and the Botrytis cinerea pectin lyase BcPNL1 in modulating Arabidopsis thaliana susceptibility.","authors":"Antoine Davière, Aline Voxeur, Sylvie Jolivet, Luka Lelas, Samantha Vernhettes, Marie-Christine Soulié, Mathilde Fagard","doi":"10.1038/s42003-025-07642-7","DOIUrl":"https://doi.org/10.1038/s42003-025-07642-7","url":null,"abstract":"In this study, we investigated the interplay between nitrogen nutrition and the pectin degradation dynamics during Arabidopsis and Botrytis interaction. Our findings revealed that infected detached leaves from nitrogen-sufficient plants released more pectin lyase (PNL)-derived oligogalacturonides compared to nitrogen-deficient ones. We then focused on BcPNL1, the most highly expressed Botrytis PNL upon infection. Using mutant strains lacking BcPNL1, we observed reduced pathogenicity, a delay in germination and a lag in triggering the plant defense response. Additionally, in nitrogen-sufficient detached leaves, the elevated expression of jasmonic acid repressor genes observed upon infection with the wild-type strain was abolished with the mutants. These results linked the increased production of BcPNL-derived products to the increased expression of jasmonic acid repressor genes, contributing partially to the higher susceptibility of nitrogen-sufficient detached leaves. These findings could lay the foundation for new strategies aimed at reconciling both crop resistance to pathogens and the improvement of nitrogen nutrition.","PeriodicalId":10552,"journal":{"name":"Communications Biology","volume":"8 1","pages":"204"},"PeriodicalIF":5.2,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isoacteoside alleviates hepatocellular carcinoma progression by inhibiting PDHB-mediated reprogramming of glucose metabolism.

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-02-08 DOI: 10.1038/s42003-025-07622-x

Lijun Zhao, Haonan Qi, Weiting Liu, Huiying Lv, Peixian Li, Wenyue Liu, Ruili Sun, Qiongzi Wang, Xiangpeng Wang

{"title":"Isoacteoside alleviates hepatocellular carcinoma progression by inhibiting PDHB-mediated reprogramming of glucose metabolism.","authors":"Lijun Zhao, Haonan Qi, Weiting Liu, Huiying Lv, Peixian Li, Wenyue Liu, Ruili Sun, Qiongzi Wang, Xiangpeng Wang","doi":"10.1038/s42003-025-07622-x","DOIUrl":"https://doi.org/10.1038/s42003-025-07622-x","url":null,"abstract":"Pyruvate dehydrogenase B (PDHB) is an important component of the pyruvate dehydrogenase complex and is implicated in altering tumor metabolism and promoting malignancy. However, the specific impact of PDHB on hepatocellular carcinoma (HCC) metabolic reprogramming and its role in tumor progression remain to be elucidated. In our investigation, we have discerned a pronounced elevation in PDHB expression within HCC, intricately linked to delayed tumor staging, heightened tumor grading, and diminished prognostic outcomes. PDHB overexpression drives tumor growth and metastasis in vitro and in vivo. Mechanistically, PDHB mediates metabolic reprogramming by binding to the promoter regions of SLC2A1, GPI, and PKM2, promoting glycolysis-related gene transcription, contributes to HCC sorafenib resistance. In addition, Isoacteoside is a targeted inhibitor of PDHB and exert antitumor effects on HCC. In the mouse xenograft model, the combination of isoacteoside and sorafenib shows significantly better effects than sorafenib alone. In summary, our study validates PDHB as an oncogenic drug resistance-related gene capable of predicting HCC tumor progression. PDHB and Isoacteoside could be potential avenues for targeted and combination therapies in liver cancer.","PeriodicalId":10552,"journal":{"name":"Communications Biology","volume":"8 1","pages":"205"},"PeriodicalIF":5.2,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Author Correction: GATA4 binding to the Sox9 enhancer mXYSRa/Enh13 is critical for testis differentiation in mouse.

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-02-08 DOI: 10.1038/s42003-025-07618-7

Yuya Ogawa, Iku Tsuchiya, Shogo Yanai, Takashi Baba, Ken-Ichirou Morohashi, Takehiko Sasaki, Junko Sasaki, Miho Terao, Atsumi Tsuji-Hosokawa, Shuji Takada

引用次数: 0

TEFM facilitates transition from RNA synthesis to DNA synthesis at H-strand replication origin of mtDNA.

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-02-08 DOI: 10.1038/s42003-025-07645-4

Shigeru Matsuda, Masunari Nakayama, Yura Do, Takashi Ishiuchi, Mikako Yagi, Sjoerd Wanrooij, Kazuto Nakada, Fan-Yan Wei, Kenji Ichiyanagi, Hiroyuki Sasaki, Dongchon Kang, Takehiro Yasukawa

{"title":"TEFM facilitates transition from RNA synthesis to DNA synthesis at H-strand replication origin of mtDNA.","authors":"Shigeru Matsuda, Masunari Nakayama, Yura Do, Takashi Ishiuchi, Mikako Yagi, Sjoerd Wanrooij, Kazuto Nakada, Fan-Yan Wei, Kenji Ichiyanagi, Hiroyuki Sasaki, Dongchon Kang, Takehiro Yasukawa","doi":"10.1038/s42003-025-07645-4","DOIUrl":"https://doi.org/10.1038/s42003-025-07645-4","url":null,"abstract":"Transcription of human mitochondrial DNA (mtDNA) begins from specific transcription promoters. In strand-asynchronous mtDNA replication, transcripts from the light-strand promoter serve as primers for leading-strand synthesis at the origin of the H-strand replication (OH). A 7S DNA strand, a presumed aborted replication product, is also synthesized from OH. Transition from RNA synthesis to DNA synthesis at OH is crucial for balancing replication with transcription, yet the mechanism remains unclear. Herein, we examine the role of mitochondrial transcription elongation factor (TEFM) in this process. TEFM knockout results in decreased 7S DNA, strand-asynchronous replication intermediates, and mtDNA copy number, all of which are concordant with downregulation of RNA-to-DNA transition at OH. Conversely, levels of tRNAs encoded near transcription promoters increase, indicating enhanced transcription initiation frequency. Taken together, we propose that, in addition to conferring processivity to the mitochondrial RNA polymerase, TEFM plays a crucial role in maintaining the balance between mitochondrial transcription and replication.","PeriodicalId":10552,"journal":{"name":"Communications Biology","volume":"8 1","pages":"202"},"PeriodicalIF":5.2,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Densely vascularized thick 3D tissue shows enhanced protein secretion constructed with intermittent positive pressure.

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-02-08 DOI: 10.1038/s42003-025-07627-6

Misako Katsuura, Jun Homma, Yuhei Higashi, Hidekazu Sekine, Tatsuya Shimizu

{"title":"Densely vascularized thick 3D tissue shows enhanced protein secretion constructed with intermittent positive pressure.","authors":"Misako Katsuura, Jun Homma, Yuhei Higashi, Hidekazu Sekine, Tatsuya Shimizu","doi":"10.1038/s42003-025-07627-6","DOIUrl":"https://doi.org/10.1038/s42003-025-07627-6","url":null,"abstract":"Constructing a dense vascular endothelial network within engineered tissue is crucial for successful engraftment. The present study investigated the effects of air-compressing intermittent positive pressure (IPP) on co-cultured mesenchymal stem cells and vascular endothelial cells and evaluated the potential of IPP-cultured cell sheets for transplantation therapy. The results demonstrated that the IPP (+) group exhibited a denser vascular endothelial network and significantly increased cell sheet thickness compared to the IPP (-) group. Furthermore, in vivo experiments showed that IPP-cultured cell sheets enhanced the secretion of Gaussian luciferase by genetically modified mesenchymal stem cells. These findings highlight the IPP method as a technique that simultaneously enables the thickening of planar tissues and the construction of vascular networks. This approach demonstrates promise for fabricating functional, transplantable, and thick tissues with dense vascularization and a high capacity for protein secretion, paving the way for novel applications in regenerative medicine.","PeriodicalId":10552,"journal":{"name":"Communications Biology","volume":"8 1","pages":"201"},"PeriodicalIF":5.2,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of CRISPR-Cas13b RNA base editing approaches for USH2A-associated inherited retinal degeneration.

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-02-08 DOI: 10.1038/s42003-025-07557-3

Lewis E Fry, Lauren Major, Ahmed Salman, Lucy A McDermott, Jun Yang, Andrew J King, Michelle E McClements, Robert E MacLaren

{"title":"Comparison of CRISPR-Cas13b RNA base editing approaches for USH2A-associated inherited retinal degeneration.","authors":"Lewis E Fry, Lauren Major, Ahmed Salman, Lucy A McDermott, Jun Yang, Andrew J King, Michelle E McClements, Robert E MacLaren","doi":"10.1038/s42003-025-07557-3","DOIUrl":"https://doi.org/10.1038/s42003-025-07557-3","url":null,"abstract":"CRISPR-Cas13 systems have therapeutic promise for the precise correction of point mutations in RNA. Using adenosine deaminase acting on RNA (ADAR) effectors, A-I base conversions can be targeted using guide RNAs (gRNAs). We compare the Cas13 effectors PspCas13b and Cas13bt3 for the repair of the gene USH2A, a common cause of inherited retinal disease and Usher syndrome. In cultured cells, we demonstrate up to 80% efficiency for the repair of the common c.11864 G > A and its murine equivalent c.11840 G > A, across different gRNAs and promoters. We develop and characterize a mouse model of Usher syndrome carrying the c.11840 G > A mutation designed for the evaluation of base editors for inherited retinal disease. Finally, we compare Cas13 effectors delivered via AAV for the repair of Ush2a in photoreceptors. Mean RNA editing rates in photoreceptors across different constructs ranged from 0.32% to 2.04%, with greater efficiency in those injected with PspCas13b compared to Cas13bt3 constructs. In mice injected with PspCas13b constructs, usherin protein was successfully restored and correctly localized to the connecting cilium following RNA editing. These results support the development of transcriptome targeting gene editing therapies for retinal disease.","PeriodicalId":10552,"journal":{"name":"Communications Biology","volume":"8 1","pages":"200"},"PeriodicalIF":5.2,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiomics identify the gene expression signature of the spinal cord during aging process.

IF 5.2 1区生物学

Communications Biology Pub Date : 2025-02-07 DOI: 10.1038/s42003-025-07475-4

Lintao Xu, Jingyu Wang, Jinjie Zhong, Weiwei Lin, Gerong Shen, Ning He, Xingjia Mao, Chunyan Fu, Zhaobo Huang, Fengdong Zhao, Xin Ye, Yongjian Zhu, Mingzhi Zheng, Hui Li, Lin-Lin Wang, Kai Zhong, Lijun Zhu, Ying-Ying Chen

{"title":"Multiomics identify the gene expression signature of the spinal cord during aging process.","authors":"Lintao Xu, Jingyu Wang, Jinjie Zhong, Weiwei Lin, Gerong Shen, Ning He, Xingjia Mao, Chunyan Fu, Zhaobo Huang, Fengdong Zhao, Xin Ye, Yongjian Zhu, Mingzhi Zheng, Hui Li, Lin-Lin Wang, Kai Zhong, Lijun Zhu, Ying-Ying Chen","doi":"10.1038/s42003-025-07475-4","DOIUrl":"https://doi.org/10.1038/s42003-025-07475-4","url":null,"abstract":"Age-related long-term disability is attracting increasing attention due to the growing ageing population worldwide. However, the current understanding of the senescent spinal cord remains insufficient. Bulk RNA sequencing reveals that 526 genes are upregulated and 300 genes are downregulated in senescent spinal cords. Pathway enrichment analysis of differentially expressed genes shows that senescence in spinal cords is related to phagosome function, neuroinflammation, ferroptosis, and necroptosis. Prediction of upstream transcription factors and interactome analysis identify Spi1 as a transcription factor that potentially plays a core role in senescent spinal cords. Spatial transcriptomics illustrates the spatial distribution of the transcriptomic landscape in both young and senescent spinal cords and identifies distinct neuronal and glial subtypes. The ferroptosis-associated gene Fth1 is upregulated in aged spinal cords. Flow cytometry reveals increased accumulation of free Fe2+ and ROS in senescent mixed glial cells; however, CCK-8 assays reveal that these cells are resistant to ferroptosis. SiRNA and lentivirus experiments indicate that the overexpression of Fth1 in normal mixed glial cells reduces their sensitivity to ferroptosis, whereas Fth1 knockdown increases their sensitivity to ferroptosis. In summary, spatial and bulk transcriptomics elucidate the transcriptional characteristics of young versus senescent spinal cords, thus highlighting the role of Fth1 in mediating ferroptosis resistance in senescent mixed glial cells.","PeriodicalId":10552,"journal":{"name":"Communications Biology","volume":"8 1","pages":"193"},"PeriodicalIF":5.2,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0