Combinatorial chemistry & high throughput screening最新文献

{"title":"Liuwei Dihuang Prevents Human Umbilical Vein Endothelial Cells Senescence via the JPX-STING-IRF3 Pathway","authors":"Chao Xu, Tiantian Cai, Xinghai Du","doi":"10.2174/1386207326666230901163717","DOIUrl":"10.2174/1386207326666230901163717","url":null,"abstract":"<p><strong>Background: </strong>Cellular senescence plays a crucial role in age-related diseases. Endothelial senescence is closely associated with age-related vascular disorders. This study aimed to reveal the role of traditional Chinese medicine Liuwei Dihuang (LWDH) in anti-endothelial cell senescence.</p><p><strong>Methods: </strong>Human umbilical vein endothelial cells (HUVECs) were exposed to LPS treatment to induce senescence. Senescence-associated β-galactosidase (SA-β-gal) positive staining, p53 and p16 expression, BrdU staining, and relative telomere length (RTL) experiments were conducted to estimate LPS-induced cellular senescence of HUVECs. Real-time qPCR analysis was performed to identify differentially expressed lncRNAs in LPS-induced senescent HUVECs before and after treatment with LWDH. Bioinformatics analysis and ChIP assay were conducted to explore the mechanism of JPX in the anti-endothelial cell aging effect of LWDH.</p><p><strong>Results: </strong>We first discovered that lncRNA JPX and STING-IRF3 pathways are involved in the process of anti-endothelial senescence of LWDH. Mechanistically, LWDH could reverse abnormally elevated JPX induced by LPS and inhibit the activation of STING, as well as the interaction between JPX and STING.</p><p><strong>Conclusion: </strong>Overall, our study explores the potential therapeutic value of LWDH and provides key insights into the potential avenues for preventing and treating HUVECs senescence.</p>","PeriodicalId":10491,"journal":{"name":"Combinatorial chemistry & high throughput screening","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10181934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review of Research on the Utilization of Ligularia Plants Based on their Functional Compositions.","authors":"Yaqiong Wang, Weifeng Dai, Cheng Yuan, Mengyang Liu, Jingyuan Wen, Mi Zhang","doi":"10.2174/0113862073367895250324054212","DOIUrl":"https://doi.org/10.2174/0113862073367895250324054212","url":null,"abstract":"<p><p>The genus Ligularia belongs to the family Asteraceae, with approximately 150 species worldwide. It is primarily distributed from Europe and the Himalayas to Japan, and it is rich in resources, with many species possessing medicinal value. According to the research reports on the functional compositions, the research progress and resource utilization of this genus were summarized from 2016 to the present. This paper aims to provide some references for the basic research results of the genus to industrialization. In general, after 2016, combined with the work of chemical and active investigation, some varieties have been applied and explored in drugs, cosmetics, food, daily necessities, pesticides, and feed, reflecting great development and value.</p>","PeriodicalId":10491,"journal":{"name":"Combinatorial chemistry & high throughput screening","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143982764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Content Screening of Wind-Dampness Dispelling Traditional Chinese Medicines against Podocyte EMT Induced by IgA1.","authors":"Jin Yu, Lingli Zhu, Yue Sun, Caifeng Zhu","doi":"10.2174/0113862073350741250410053000","DOIUrl":"https://doi.org/10.2174/0113862073350741250410053000","url":null,"abstract":"<p><strong>Aims: </strong>Immunoglobulin A (IgA) Nephropathy (IgAN) is characterized by pIgA1 deposition in the glomerular mesangium, resulting in podocyte Epithelial-to-Mesenchymal Transition (EMT). Traditional Chinese Medicines (TCMs) have been used for the treatment of IgAN for several years and have demonstrated positive efficacy. The present study aimed to establish a High-Content Screening (HCS) method for identifying wind-dampness dispelling TCM extracts that can mitigate podocyte EMT induced by pIgA1.</p><p><strong>Material and methods: </strong>IgA1 from IgAN patients was used to establish a podocyte EMT model. The expression of EMT markers, including desmin, podocalyxin, and podocin, was assessed by Immunocytofluorescence (IF). An image-based HCS method was established to identify winddampness dispelling TCMs with the anti-EMT activity of podocyte EMT by automatic acquisition and processing of dual-fluorescent labeled images.</p><p><strong>Results: </strong>A total of 21 wind-dampness-dispelling TCM extracts were screened using the HCS system, leading to the identification of eight wind-dampness-dispelling TCM extracts exhibiting anti-EMT activity. These eight extracts inhibited the pIgA-induced expression of desmin while upregulating the expression of podocalyxin and podocin.</p><p><strong>Conclusion: </strong>By quantifying the changes in the expression of EMT markers during pIgA1- induced EMT, this study successfully identified wind-dampness dispelling TCM extracts with anti-EMT properties using an HCS system. In addition, the proposed approach presents a novel avenue for the identification of wind-dampness-dispelling TCMs for the treatment of pIgA1- induced EMT.</p>","PeriodicalId":10491,"journal":{"name":"Combinatorial chemistry & high throughput screening","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143981481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Efficacy and Mechanism of Astragalus Polysaccharide in Treating Allergic Asthma through Network Pharmacology, Bioinformatics, and Experimental Verification.","authors":"Linhan Hu, Haiyun Zhang, Yihang Zhang, Lei Wang, Honglei Zhang, Juntong Liu, Linpeng Cong, Yumei Zhou, Ji Wang, Qi Wang","doi":"10.2174/0113862073368307250409055727","DOIUrl":"https://doi.org/10.2174/0113862073368307250409055727","url":null,"abstract":"<p><strong>Background: </strong>Allergic asthma is an inflammatory disease of the airways that causes great distress to the patient's normal life. Astragalus Polysaccharide (APS) is the main active ingredient in the traditional Chinese medicine Astragalus mongholicus Bunge, which has the effect of regulating immune function.</p><p><strong>Objective: </strong>This study aimed to evaluate the effect of APS on allergic asthma and investigate its potential mechanism of action.</p><p><strong>Methods: </strong>This study utilized network pharmacology to predict the relevant targets and signaling pathways of APS treatment for allergic asthma. Subsequently, an animal model was established using Ovalbumin (OVA) induction. The efficacy of APS was verified using histopathologic staining and Airway Hyperresponsiveness (AHR) assay. Signaling pathways were examined using Western Blot (WB). Finally, bioinformatics analysis was utilized to explore the correlation between the progression of allergic asthma and signaling pathways.</p><p><strong>Results: </strong>Network pharmacology analysis identified 15 intersection targets significantly enriched in the PI3K/AKT signaling pathway. The results of molecular docking showed that small molecule drugs have a strong binding ability to target proteins. The experiments confirmed APS successfully suppressed the pathological symptoms in allergic asthma model mice. Subsequently, WB provided evidence supporting that APS has potential therapeutic effects mediated through the PI3K/AKT signaling pathway. The bioinformatics results confirmed that disease progression in allergic asthma patients does correlate with the PI3K/AKT signaling pathway.</p><p><strong>Conclusion: </strong>Our study suggests that APS may treat allergic asthma by targeting the PI3K/AKT signaling pathway. This provides a basis for preliminary research on the clinical application of APS for treating allergic asthma.</p>","PeriodicalId":10491,"journal":{"name":"Combinatorial chemistry & high throughput screening","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Effects and Molecular Mechanism of Banxia Xiexin Decoction on Intestinal Mucosal Barrier Function in Sepsis.","authors":"Wen Dai, Lei Zhou, Hao Hao, Diankui Wang, Feihu Zhang, Peng Wang, Lin Wang, Li Kong","doi":"10.2174/0113862073349597250410111941","DOIUrl":"https://doi.org/10.2174/0113862073349597250410111941","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Aim: &lt;/strong&gt;This study aims to investigate therapeutic effects and involved molecular mechanisms of Banxia Xiexin Decoction (BXD) in reducing gastrointestinal complications associated with sepsis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Sepsis is a common critical illness that threatens patient survival and costs society a lot. This syndrome is a prominent cause of death in ICUs due to its high mortality rate, which exceeds 30% after 28 days and 35.5% after 90 days. Sepsis remains a major medical challenge despite a 20%-30% drop in fatality rates due to a better understanding of its physiological and pathological features and better therapeutic techniques. There is no pharmacological treatment for sepsis, highlighting the need for more study.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;We explored the protecting effects of BXD on intestinal functionality in sepsis by investigating its roles in the regulation of mitochondrial autophagy and mitochondrial functioning in small intestinal epithelial cells, primarily via the PINK1/Parkin signaling pathway.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Method: &lt;/strong&gt;We established a cell model of Human Intestinal Epithelial Cell (HIEC) injury induced by lipopolysaccharide (LPS) and a cecal ligation and perforation (CLP) sepsis model in Sprague Dawley (SD) rats. The cell model and animal model of sepsis were divided into control groups and different treatment groups that received different doses of BXD. We utilized HIECs with PINK1 knockdown to assess BXD's protective effects on the sepsis intestinal barrier and its regulatory mechanism both on the PINK1/Parkin signaling pathway, exploring both its facilitative and inhibitory effects. ELISA method was used to measure inflammatory markers IL-6, IL-1β, and intestinal injury-related molecules IFABP and DAO. Pathological assessments were performed with H&E staining, and tight junction proteins ZO-1 and Occludin were detected using immunohistochemical staining. Mitochondrial membrane protein TOM20 was detected through immunofluorescence staining. Mitochondrial membrane potential and autophagy were assessed via flow cytometry. The expression levels of PINK1, Park, LC3, and p62 proteins and mRNA, integral to the PINK1/Parkin autophagy pathway, were evaluated using Western Blot and RT-PCR.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Compared to the control group, BXD therapy significantly lowered serum DAO, IFABP, and DA. The BXD therapy group showed a more significant and sustained drop in IL-6 and IL-1β levels than the control group. The BXD therapy reduced intestinal mucosa damage by lowering DAO and IFABP. BXD also restored tight junction proteins ZO-1 and Occludin, improving intestinal mucosal barrier function. In septic rats, BXD therapy lowered serum IL-6 and IL-1β levels, avoiding inflammation and reducing intestinal damage. BXD enhanced TOM20, which protected intestinal epithelial cell mitochondria against decreasing mitochondrial membrane potential. BXD increased the PINK1/Parkin mitochondrial ","PeriodicalId":10491,"journal":{"name":"Combinatorial chemistry & high throughput screening","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the Potential of Ketamine: A Systematic Review and Meta-analysis of its Safety and Efficacy in Acute Pain Management.","authors":"Xiaojuan Chou, Wenhua Zha, Jian Hu, Chen Chen, Rajesh K Singh, Geng Liu","doi":"10.2174/0113862073377616250404101442","DOIUrl":"https://doi.org/10.2174/0113862073377616250404101442","url":null,"abstract":"<p><strong>Background: </strong>The management of acute pain is a crucial and challenging component of emergency care. The pursuit of an ideal drug that alleviates pain rapidly and with fewer side effects is an ongoing endeavor.</p><p><strong>Objective: </strong>This meta-analysis reviews the safety and efficacy of ketamine in adult emergency department (ED) patients experiencing acute pain.</p><p><strong>Methodology: </strong>This study was limited to randomized controlled trials (RCTs). The comparative group was morphine or other opioids or placebo, whereas the experimental group was ketamine. The primary outcome measures, in addition to adverse events, were the numeric rating scale (NRS). The included studies were subjected to analysis using the Review Manager Database. The non-significant changes in pain score were observed in the ketamine group at 10 minutes [- 0.46 (-2.03, 1.10)], 30 minutes [-0.13 (-0.62, 0.37)], and 60 minutes [-0.18 (-0.97, 0.61)] as compared to the control group.</p><p><strong>Results: </strong>The significant changes were observed at 15 minutes [-4.11 (-7.91, -0.31)] in the ketamine group as compared to the control group. The overall risk ratio (1.20 [95% Confidence interval (CI), 0.93 to 1.55] indicated a non-significant difference in adverse events in the control group as compared to the ketamine group. The heterogeneity among included studies was found to be higher, as indicated by the I2 statistics.</p><p><strong>Conclusion: </strong>There were no significant differences in adverse events between the ketamine and the control groups. More randomized clinical trials are needed to determine ketamine's involvement in acute pain at 10, 30, and 60 minutes.</p>","PeriodicalId":10491,"journal":{"name":"Combinatorial chemistry & high throughput screening","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Active Ingredients and Core Targets of Erxia Decoction in the Treatment of Sleep Disorder by Integration of Network Pharmacology and Proteomics.","authors":"Bo Jiang, Huiran Yang, Fei Zhou, Huijun Qu, Xueqian Hu, Zhanwen Liu","doi":"10.2174/0113862073348555250320035548","DOIUrl":"https://doi.org/10.2174/0113862073348555250320035548","url":null,"abstract":"<p><strong>Aims: </strong>For clarifying the \"multi genes and multi targets\" characteristic of the treatment of Erxia Decoction (EXD), the aim of this study was to employ network pharmacology technology to perform cluster analysis on selected EXD targets.</p><p><strong>Background: </strong>EXD, a famous Chinese herbal prescription, consisting of Pinelliae Rhizoma (PR) and Prunellae Spica (PS), was mainly used to treat sleep disorder (SLD).</p><p><strong>Objective: </strong>Using network pharmacology combined with proteomics to find out the main active components and core targets of EXD in the treatment of SLD.</p><p><strong>Method: </strong>By constructing the network of drug-component-target, the key protein targets of EXD for the treatment of SLD were screened. Then the interaction of the main active components of EXD and predicted candidate targets were verified. Then the proteomic analysis was used to screen the core targets in BV2 cells treated with EXD or the chemical ingredients, and the expression level was validated by Western blotting. Finally, molecular docking was used to further evaluate the mechanism of the action of the main ingredients and the core targets.</p><p><strong>Result: </strong>The 24 components of EXD mainly participate in the SLD treatment process by acting on 15 important key genes, and the core signal pathways were identified in the process of the action of EXD in treating SLD. Four key ingredients and five core targets were revealed from the results of network pharmacological analysis combination with proteomics, and then the AKT1 protein as a key target was validated by PCR and Western blotting.</p><p><strong>Conclusion: </strong>This study preliminarily revealed EXD, morin (MOR) and quercetin (QUE) mainly inhibited the AKT1 core targets for the treatment of SLD using the network pharmacological analysis, proteomics, Western blotting, and molecular docking. The results elucidated partly the molecular mechanism and provided clues and a basis for further research.</p>","PeriodicalId":10491,"journal":{"name":"Combinatorial chemistry & high throughput screening","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness and Safety of Butyphthalide Injection in Treatment of Aphasia after Cerebral Infarction: A Meta-analysis.","authors":"Xiongying Huang, Yuan Xie, Yingchen Li","doi":"10.2174/0113862073368524250313053657","DOIUrl":"https://doi.org/10.2174/0113862073368524250313053657","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study is to evaluate the efficacy and safety of butylphthalide injection in the treatment of aphasia after cerebral infarction.</p><p><strong>Methods: </strong>CNKI, Wanfang database, Duxiu, VIP, CBM, PubMed, Embase, Web of Science, Cochrane, and Socolar databases were searched from the establishment of the database to August 2024. According to the topic of butylphthalide injection treated aphasia after cerebral infarction, the eligible RCT was selected and meta-analysis was performed using RevMan 5.4 software and stata16 software. Two researchers independently screened the study, extracted the basic information in the study, and assessed the risk of bias in the study.</p><p><strong>Results: </strong>A total of 1047 patients were included in 12 articles, including 524 patients in the experimental group (butylphthalide injection + conventional/special speech rehabilitation training) and 523 patients in the control group (conventional/special speech rehabilitation). The results of meta-analysis showed that the total clinical efficacy of the experimental group was better than that of the control group (RR=1.29, 95%CI: 1.17-1.43, Z=4.98, P < 0.0000). The National Institutes of Health Stroke Scale (NIHSS) score of the experimental group was lower than that of the control group (conventional language rehabilitation training group: MD=-4.11,95%CI: -4.44~- 3.78, Z=24.38, P < 0.00001; special language rehabilitation training group: MD=-2.53, 95%CI: - 3.77 ~ -1.28, Z=3.97, P < 0.00001). Aphasia Quotient (AQ) scores of experimental groups were higher than those of control group (conventional language rehabilitation training group: MD=11.10, 95%CI: 10.26 ~ 11.94, Z=25.97, P<0.00001; special language rehabilitation training group: MD=5.23, 95%CI: 3.34~7.11, Z=5.44, P<0.00001); Adverse reactions: P>0.05, no statistical significance.</p><p><strong>Conclusion: </strong>Butylphthalide injection is effective and safe in the treatment of aphasia after cerebral infarction.</p>","PeriodicalId":10491,"journal":{"name":"Combinatorial chemistry & high throughput screening","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Potential Bifunctional Peptides with Anti-tyrosinase and Antioxidant Activities from Porphyra Protolysate Using in Silico Analysis.","authors":"Si-Bo Huang, Pei-Xia Feng, Liu-Ying Li, Hong-Hui Guo, En-Qin Xia","doi":"10.2174/0113862073341023250113053959","DOIUrl":"https://doi.org/10.2174/0113862073341023250113053959","url":null,"abstract":"<p><strong>Background: </strong>Extracts from Porphyra have been detected to have antioxidant activity and tyrosinase (TYR) inhibitory activity. However, bioactive peptides (BPs) released from Porphyra proteins (PPs) have not been comprehensively reported.</p><p><strong>Objective: </strong>The aim of this study is to rapidly identify bifunctional peptides with antioxidant and TYR inhibitory activities from a large number of digested peptides from PPs.</p><p><strong>Methods: </strong>In this study, a total of 3,288 proteins from six main species of Porphyra were collected, and the antioxidant potential (AP) was evaluated. Hydrolyzed peptides with 2-8 amino acid lengths were collected and known antioxidants were removed. Next, these peptides were further screened using ADMET analysis. Finally, the DPPH· scavenging potential (IC50) and TYR inhibition potential (TIP) of these peptides were further predicted by QSAR models and molecular docking based pharmacophore models, respectively.</p><p><strong>Results: </strong>The most released antioxidant peptides after digestion of all types of PPs were dipeptides with sequences EL, IR and AY. In addition, 44,689 short non-repeatable peptides were swirled in these hydrolysates, which have not yet been reported to have antioxidant activity. Next, 337 of these digested peptides were predicted to be absorbed without hepato-renal toxicity and had virtual metabolic scores > 0.01%. Finally, 138 peptides were predicted to have AP and TIP.</p><p><strong>Conclusion: </strong>Porphyra is a kind of promising source rich in bifunctional peptides. Present study adopted an innovative method with some free scripts to rapid discovery of bifunctional peptides from a large number of unknown PPs.</p>","PeriodicalId":10491,"journal":{"name":"Combinatorial chemistry & high throughput screening","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the Microbiome-Gut-Eye Axis: A Mendelian Randomization Analysis of the Causal Influence of Gut Microbiota on Myopia.","authors":"Weicheng Xu, Wei Shi","doi":"10.2174/0113862073385717250415110224","DOIUrl":"https://doi.org/10.2174/0113862073385717250415110224","url":null,"abstract":"<p><strong>Background: </strong>The intricate relationship between the gut microbiome and myopia is increasingly recognized, underscoring the need to explore its causal dynamics. Despite emerging evidence, the influence of Gut Microbiota (GM) on ocular development remains underexplored.</p><p><strong>Methods: </strong>This study utilized Mendelian Randomization (MR) to investigate the causal impact of GM on the development of myopia. Instrumental variables (IVs) were identified from Genome- Wide Association Studies (GWAS), focusing on genetic variants significantly associated with microbiome composition. A comprehensive array of MR techniques was applied to ensure a robust estimation of causal effects and to adjust for potential confounders and pleiotropy.</p><p><strong>Results: </strong>The Inverse-Variance Weighted (IVW) method was used to identify significant associations between GM and myopia. Increased risk of myopia was linked to the class Betaproteobacteria (OR=1.01, 95% CI 1.004-1.017, P=0.003), the order Burkholderiales (OR=1.009, 95% CI 1.001-1.016, P=0.02), the family Oxalobacteraceae (OR=1.005, 95% CI 1.001-1.01, P=0.023), and several genera including Eubacterium xylanophilum group (OR=1.007, 95% CI 1.001-1.013, P=0.033), and Bifidobacterium (OR=1.005, 95% CI 1-1.01, P=0.038). Protective effects were noted for the order Mollicutes RF9 (OR=0.994, 95% CI 0.99-0.999, P=0.014), the genus Allisonella (OR=0.996, 95% CI 0.993-0.999, P=0.019), the genus Lachnospiraceae UCG001 (OR=0.994, 95% CI 0.989-1, P=0.045), and the family Enterobacteraceae (OR=0.991, 95% CI 0.982-1, P=0.047) and order Enterobacteriales (OR=0.991, 95% CI 0.982-1, P=0.047). Sensitivity analyses further confirmed the robustness of these findings.</p><p><strong>Conclusions: </strong>The findings support the \"Microbiome-Gut-Eye Axis\" as a potential factor in myopia pathogenesis and highlight microbiota-targeted interventions as novel therapeutic strategies for managing myopia. This study lays the groundwork for further research on how modifying GM can influence eye health and offers new perspectives on preventive health strategies.</p>","PeriodicalId":10491,"journal":{"name":"Combinatorial chemistry & high throughput screening","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143982771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}