Experimental and Applied Biomedical Research (EABR)最新文献

{"title":"Restoration of Meibomian Gland Functionality with Novel Mesenchymal Stem Cell-Derived Product “Derived-Multiple Allogeneic Proteins Paracrine Signaling (d-MAPPS)”: A Case Report","authors":"C. Harrell, V. Volarevic","doi":"10.2478/sjecr-2020-0059","DOIUrl":"https://doi.org/10.2478/sjecr-2020-0059","url":null,"abstract":"Abstract Meibomian gland dysfunction (MGD) results in the increased tear film osmolarity and leads to the development of dry eye disease. Results obtained in several experimental and clinical studies suggested that mesenchymal stem cells (MSCs) could promote repair and regeneration of injured meibomian glands. We recently developed a new biological product “derived-Multiple Allogeneic Proteins Paracrine Signaling (d-MAPPS)” which activity was based on the effects of immunosuppressive and trophic factors secreted by MSCs. Herewith, we report a case of MGD treated by d-MAPPS containing eye drops, demonstrating therapeutic potential of d-MAPPS in regeneration of injured meibomian glands and in the attenuation of MGD. D-MAPPS containing eye drops significantly attenuated MGD-related symptoms (foreign body sensation, burning, pain in the eye and eye fatigue) and remarkably improved quality of life. The analysis of meibomian glands demonstrated restoration of meibomian gland morphology, structure and function, after the 3-weeks of d-MAPPS based therapy. MGD patient did not report any adverse effects related to the d- MAPPS administration, indicating that d-MAPPS containing eye drops were safe for intraocular application.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125885649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Image and Laboratory Aspects of Carotid Atherosclerosis","authors":"Marieta Peycheva, T. Deneva, D. Zlatareva, T. Zdravkova, Lubomir Chervenkov, Zdrvaka Harizanova","doi":"10.2478/sjecr-2022-0047","DOIUrl":"https://doi.org/10.2478/sjecr-2022-0047","url":null,"abstract":"Abstract Carotid atherosclerosis is a main risk factor for ischemic stroke. Plaque instability is determined by the morphological characteristics of the plaque and can be characterized by immunological biomarkers. The study aimed to examine the connection between serum levels of hs-CRP, fibrinogen, ICAM-1, VCAM-1 and carotid atherosclerosis and the different types of atherosclerotic plaques imaged by ultrasound and magnetic resonance. The study involved 120 patients with carotid atherosclerosis and 33 patients without carotid atherosclerosis. Blood samples were collected to analyze the serum level of hs-CRP, fibrinogen, ICAM-1 and VCAM-1. The ultrasound analysis included detection of atherosclerotic plaques in the internal carotid arteries, measurement of artery stenosis in percentage and determination of plaque types by the classification of Gray-Weales/Gerolacus. A small subset of 30 patients with carotid atherosclerosis performed 3T magnetic resonance imaging. Atherosclerotic plaques were classified into 8 types based on the modified MR classification of the American Heart Association. Significantly higher serum levels of hs-CRP (p <0.001) and fibrinogen (p = 0.018) were observed in patients with carotid atherosclerosis compared to patients without atherosclerosis. Criterion values for hs-CRP > 4.13mg/l and for fibrinogen > 3.6 g/l were associated with the presence of carotid plaques with accuracy of 70%. No relation was observed between the investigated biomarkers, the artery stenosis and the types of atherosclerotic plaques determined by ultrasound and magnetic resonance diagnostic methods. Hs-CRP and fibrinogen are reliable serum markers whose increased serum concentrations are connected with the presence of carotid atherosclerosis.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"214 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115504870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Electrochemotherapy on Breast Cancer Cell Lines","authors":"D. Cvetković, A. Cvetkovic, N. Filipovic","doi":"10.2478/sjecr-2019-0073","DOIUrl":"https://doi.org/10.2478/sjecr-2019-0073","url":null,"abstract":"Abstract Despite advances in treatment, breast cancer remains one of the leading causes of death, and obviously new approaches to the treatment are needed. Due to minimal side effects, unlike more aggressive forms of therapy such as chemotherapy and radiotherapy, the application of irreversible electroporation-electrochemotherapy represents a new modality in the treatment of cancer. Electrochemotherapy uses an electric field (375 V cm -1) to allow increased absorption of chemotherapeutic drugs selectively in tumor cells. Accordingly, the total dose of these agents can be significantly reduced and numerous side effects can be avoided in this way. The Real Time Cell Analysis-RTCA-xCELLigence system was used to monitor the cytotoxic effects of the treatment. The results confirmed the justification of the use of paclitaxel in chemotherapy and showed cytotoxic effects of paclitaxel which were time and dose-dependent in both cell lines. When paclitaxel was administered in combination with an electric field, in both cell lines, the results showed a greater cytotoxic effect compared to the same treatment without electrochemotherapy. MCF-7 cells are more sensitive to electrochemotherapy treatment with paclitaxel compared to MDA-MB-231. Electrochemotherapy using paclitaxel in MCF-7 cells had a 6.4-fold higher cytotoxicity compared to the treatment only with paclitaxel. The results obtained support the current knowledge of the benefits of electrochemotherapy. It has been shown that electrochemotherapy can significantly increase the effects of paclitaxel in the tested cell lines. In this way, a very high concentration of chemotherapeutics in the targeted tissue was achieved, which represents localized chemotherapy.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"55 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120895618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age and Gender Differences in Orbital Measurements Within Serbian Population in Kragujevac Region of the Republic of Serbia","authors":"Igor Jakovcevski, R. Vojinovic, I. Zivanovic-Macuzic, M. Jakovcevski","doi":"10.2478/sjecr-2020-0030","DOIUrl":"https://doi.org/10.2478/sjecr-2020-0030","url":null,"abstract":"Abstract Orbital measures are not only important parameters in planning ophthalmologic and aesthetic surgical procedures, but also significant anthropology and forensic medicine markers. Using computer tomography and subsequent multiplanar reconstruction we analyzed orbits from 75 Serbian healthy volunteers, examined in the Clinical Hospital of Kragujevac. The subjects were subclassified in age categories, namely 24-39, 40-59, 60-69 and 70-85 years of age, as well as by genders. Taken as a whole population, regardless of age, women had smaller orbital height and width and smaller volumes than men, but similar orbital indices, thus proving once again the importance of the orbital index for comparisons. Additionally, both biorbital and interorbital distances were higher in men than in women. Comparing age groups, biorbital and interorbital distances increased with aging in males, but remained constant in females. The opposite was true for orbital indices which increased significantly with age in females, but remained constant in males. Taken together, our results indicate the presence of age- and gender-related differences in orbital measures within ethnically and geographically homogenous population. It would be interesting to examine subjects from other regions to confirm the patterns reported here.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128354922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic Significance of P21 Protein in Breast Cancer","authors":"Dalibor V. Jovanovic, S. Mitrovic, Dzemila Alic, Danijela Besic, Dragan R. Knezevic, J. Dimitrijevic, Milena Ilic","doi":"10.2478/sjecr-2023-0005","DOIUrl":"https://doi.org/10.2478/sjecr-2023-0005","url":null,"abstract":"Abstract Breast cancer is the most common malignancy in women. Bearing in mind these circumstances, a review of new molecular mechanisms underlying breast cancer progression, resistance and various aspects of existing therapeutic methods would lead to new insights for biologists and clinicians. In this regard, we conducted a study covering recent advances in breast cancer biology with a focus on the p21 protein. The study included 147 patients diagnosed with invasive breast cancer. The presence of non-invasive lesions was noted in each invasive breast cancer and surrounding tissue. p21 expression was determined by reading the percentage of nuclear expression in epithelial cells of invasive breast cancer and non-invasive lesions. Results showed that expression of p21 increases with the progression of cytological changes in the epithelium; it is significantly higher in invasive breast cancer compared to non-invasive lesions (p<0.001). There is a difference in p21 expression between different molecular subtypes of breast cancer (p=0.004). Statistically significantly higher values of p21 expression were observed in those breast cancers that showed overexpression of HER2 compared to HER2-negative tumors (p=0.001). Depending on Ki67 expression, the highest p21 expression is in the group with high Ki67 expression values (p=0.019). The increase in p21 expression in tumor cells was accompanied by a statistically significantly reduced expression of ER (p=0.015, ρ=-0.225) and PR (p=0.027, ρ−0.205). p21 protein plays an important role in proliferation, malignant transformation, as well as in progression from non-invasive lesions to invasive breast cancer.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133851259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of the Acute Total Gamma Radiation in a Sublethal Dose on the Biophysical Properties of Red Blood Cells, Lipid Peroxidation, Antioxidant Supply and Hemocoagulating Properties of Erythrocytes","authors":"T. Zaporozhets, L. Korovina, O. Sanyk","doi":"10.2478/sjecr-2022-0048","DOIUrl":"https://doi.org/10.2478/sjecr-2022-0048","url":null,"abstract":"Abstract The aim of the investigation was to study the effect of acute, total gamma-irradiation in a sublethal dose on the biophysical properties of erythrocytes, the intensity of lipid peroxidation, antioxidant supply and hemocoagulating properties of erythrocytes. The experiments were carried out on 11-12-week-old age guinea pigs, males and females in equal numbers. The animals were exposed to a single total radiation at a dose of 4.5 Gy (sublethal dose, LD 50/30). The studies were carried out on the 7th day after exposure to radiation (at the height of radiation sickness). The development of radiation damage was accompanied by intense erythropoiesis and the appearance of erythrocytes with a high resistance to hemolysis and an increased sedimentation rate. After acute gamma irradiation, depletion of the antioxidant system was noted. It manifested in a decrease in the activity of superoxide dismutase of erythrocytes by 19.7% (p<0.01) and the concentration of serum ceruloplasmin by 21.5% (p<0.01). The content of thiobarbituric acid reactive substances (TBARS) and their accumulation while the incubation of erythrocytes remained within the normal range. The erythrocytes of the irradiated animals exhibited increased procoagulant and decreased antiheparin activity, which reflects conformational changes in highly radiosensitive fatty acid chains of phospholipids in their membranes. A decrease in the fibrinolytic activity of erythrocytes in irradiated animals was found.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126655276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory, But Not Excitatory Synapses Are Reduced in the Hippocampus of the Six-Month-Old Alzheimer’s Disease Model Mouse","authors":"M. Aksić, Ivona Bankovic, I. Jakovcevski, Andrea Mojsoska, S. Stanković, M. Vulovic","doi":"10.2478/sjecr-2023-0003","DOIUrl":"https://doi.org/10.2478/sjecr-2023-0003","url":null,"abstract":"Abstract Alzheimer’s disease (AD) is a devastating neurodegenerative disorder and the most common cause of elderly dementia. One of the main features of AD diseased brain are amyloid plaques, pathological depositions made of β-amyloid peptide, derived from β- amyloid precursor protein (APP). To assess how AD pathology affects synapses in the hippocampus, brain region to be one of the earliest with obvious pathological changes, we examined APPPS1 mice, transgeneticaly expressing human APP mutation (“Swedish mutation”) and human presenilin-1 mutation under the neuron-specific promoter, which develop AD symptoms early in life. We analyzed inhibitory and excitatory synapses using immunoflourescent staining and laser scanning confocal microscopy. In APPPS1 mice, inhibitory synaptic terminals labeled with vesicular inhibitory transmitter transporter (VGAT) were reduced in CA1 and CA3 regions of the hippocampus in APPPS1 mice compared to controls. This was true for both parvalbumin-positive and parvalbumin-negative terminals. On the other hand, excitatory synapses, coming from either hippocampal or entorhinal projections were similar between the genotypes. We conclude that first changes in the hippocampus caused by amyloid pathology affect inhibitory, but not excitatory synapses.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"99 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131525160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemihydranencephaly of Premature Infant - Case Report","authors":"Tijana Prodanovic, Suzana Živojinović","doi":"10.2478/sjecr-2023-0002","DOIUrl":"https://doi.org/10.2478/sjecr-2023-0002","url":null,"abstract":"Abstract Hydranencephaly represents a rare anomaly of the central nervous system and is characterized by the parenchymal absence which is replaced with a membranous sac filled with cerebrospinal liquid, glial tissue and ependyma. When it is manifested as hemihydranencephaly, the patient’s prognosis is better. We presented a case of a premature infant with hemihydranencephaly in twin pregnancy that had a fatal outcome. A premature infant in a controlled pregnancy was delivered at 31 weeks of gestational age. The mother of the newborn was hospitalized in the Clinic for Infective Diseases since she had tested positive for SARS-Covid- 19. Following an urgent Caesarean section, the newborn was tested for Covid 19 using the rapid antigen and PCR test and the results were negative. In the delivery room, the tactile stimulation, aspiration, positive pressure ventilation of the infant were applied, after which it was intubated. After the intubation, a manual heart massage was performed, after which the cardiac activity and agonal breathing movements were detected.” Three hours later, cardiorespiratory arrest happened, following unsuccessful resuscitation. The other twin was born with no anomalies, but showed signs of respiratory distress syndrome. Autopsy revealed that there was a thin-walled cavity filled with clear, yellowish liquid in the right hemisphere. Documenting cases of newborns with hydranencephaly is of a great importance for counseling parents regarding length of survival, successful management of pregnancy, and performance of prenatal diagnostic procedures.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"91 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127124745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Different Drugs with Anti-Inflamatory Potential in Prevention of Pulp Damage During the Teeth Bleaching","authors":"Miona Glisic, A. Milojević, M. Milinkovic, M. Rankovic","doi":"10.2478/sjecr-2021-0015","DOIUrl":"https://doi.org/10.2478/sjecr-2021-0015","url":null,"abstract":"Abstract This paper aims to collect all the necessary information and conclusions which deal with the anti-inflammatory drugs within the scope of teeth bleaching, and which are rooted in scientific research. It is a well-known fact that the teeth whiteners / bleachers are at the same time a very aggressive agents, which opened up the question of the safety of their application. The most frequently applied bleaching agents are the hydrogen-peroxide (H2O2) and carbim peroxide (CH6N2O3), therefore this paper will be based on the research which included these two agents. The first studies were directed towards investigating the outcome / impact on the enamel, i.e. if there is direct surface damage of the enamel layer after the use of such agents. However, bearing in mind that the clinical practice has shown that in a number of cases there arises the sensitivity of teeth in the form of painful sensation after the bleaching treatment, some researches were directed towards the estimation of the permeability of the enamel and dentin for the bleaching agent, and later the effect on the pulp tissue. Along with the existing proof about the irritability of these agents to the pulp tissue, the modern research have the idea of looking into the effects of the anti-inflamatory drugs as the accompanying and prevention therapy of any of the irreversible damage of the pulp. On one hand the application of these drugs can reduce the sensitivity and painfulness of the teeth, which makes the intervention pleasant for the patient, but on the other hand the intervention becomes completely justifiable from the aspect of safety and the basic principle primum non nocere. The anti-inflammatory drugs which will be the topic of this study are H hydrocortisone, acetaminophen, ipobruphen and etodolac. Taking into consideration the vasoconstrictive and anti-oxidative effect, the anti-inflammatory effect of the carvedilol will be looked into, which belongs to the group of beta blockers. Based on available informations it can be noticed that the most efficient anti-inflammatory effect in teeth whitening is achived by hydrocortisone.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"478 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133604765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Depression, Anxiety and Stress During the COVID-19 Pandemic Among Serbian University Students","authors":"Sofija Čolović, M. Božović, Mladen Grujicic","doi":"10.2478/sjecr-2023-0004","DOIUrl":"https://doi.org/10.2478/sjecr-2023-0004","url":null,"abstract":"Abstract Our study focused at measuring stress, anxiety, and depression among the population of university students in Serbia. The sample included 493 students from The Faculty of Mechanical Engineering and the Higher Medical School, Kraljevo, University of Kragujevac, Central Serbia. The electronic survey was completed in approximately 10 minutes. Data collection was conducted during September and October, 2022. The research instruments included: General Questionnaire (used to collect demographic and personal data before and during the COVID-19 pandemic) and Depression Anxiety Stress Scales (DASS-21). All statistical calculations were performed using the standard commercial, standard software package SPSS, version 18.0. 12.8% of students reported severe and very severe symptoms of depression. In 21.7% of cases, severe and very severe symptoms of anxiety were reported. 20.3% of students reported sever and very severe symptoms of stress. Even though there are several studies on the mental health of Serbian college and university students during the COVID-19 pandemic, our article is unique in that it observes their mental health two years after the onset of the pandemic. This allows us to compare the findings with those obtained for the onset of the pandemic.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117260230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}