Experimental and Applied Biomedical Research (EABR)最新文献

{"title":"Single-Stage Reduction of Neglected Perilunate Dislocation: A Case Report","authors":"M. Vranješ, Vedran Zigic, N. Gvozdenović","doi":"10.2478/sjecr-2022-0031","DOIUrl":"https://doi.org/10.2478/sjecr-2022-0031","url":null,"abstract":"\u0000 Neglected perilunate dislocations are rare and challenging injuries. We report a 59 days old perilunate dislocation in a 55 year old right-handed male. X-ray and CT imaging revealed a dorsal perilunate dislocation with no additional fractures and no increased density or microarchitectural collapse of the lunate or scaphoid. A single-stage open reduction and internal K-wire fixation procedure through a dorsal and volar approach was done. Eighteen months after the procedure, the patient was pain free during movement and had a ROM of 70%. His grip strength was 80.8% the opposite side and the Mayo wrist score was calculated 75. Full range of finger movement was achieved and he carried out his routine without any other complication.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"21 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140438708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Memantine Therapy as an Augmentation in Treatmentresistant Depression: A Case Report","authors":"Мilena Stojkovic, V. Janjić, Nemanja Murić, N. Zornic, Vladimir Jakovljević, V. Zivkovic, B. Radmanovic","doi":"10.2478/sjecr-2022-0054","DOIUrl":"https://doi.org/10.2478/sjecr-2022-0054","url":null,"abstract":"Abstract Evidence suggests that treatment-resistant depression (TRD), defined as the occurrence of an insufficient clinical response after adequate antidepressant therapy among patients diagnosed with major depression, is one of the most important public health problems and is associated with significant disability and psychosocial impairment. Here we present a case of depression resistance to the treatment of a 22-year-old man and the effects of Memantine augmentation. The first symptoms of depression appeared in January 2019, and pharmacotherapeutic treatment began in March of the same year. He was in a bad mood, sad, hopeless, tense, lacked energy, lost interest, and decreased libido. During his twoyear pharmacotherapeutic therapy, he took SSRIs, SNRIs, TeCAs, SARIs, atypical antipsychotics with antidepressant effects, such as aripiprazole and cariprazine. Since the patient still did not have adequate therapeutic response and new drugs were available, the choice fell on Memantine after researching several databases and collecting the data listed at the beginning of the article. The initial dose was 5 mg daily and was gradually increased by 5 mg each week to a maximum of 20 mg. After 2 weeks at a stable dose, the patient reported feeling a little better. Significant improvement in symptoms was observed in the MADRS score, which was then applied to assess the effects of augmentation. After four weeks of therapy, he entered remission which is still ongoing.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139230045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined Orthodontic and Prosthetic Treatment of a Patient with Angle Class II Division 1 Malocclusion: A Case Report","authors":"Nemanja Okičić, Marko Milosavljević, M. Jovanović, Đorđe Božović, Jelena Erić","doi":"10.2478/eabr-2023-0007","DOIUrl":"https://doi.org/10.2478/eabr-2023-0007","url":null,"abstract":"Abstract Skeletal class II division 1 malocclusion is primarily characterized by maxillary protrusion, mandibular retrusion or a combination of both. Treatment modalities for these patients are different depending on the age and the severity of the case. In adult patients with skeletal class II division 1, where the discrepancy is very severe, orthodontic and orthognathic surgery combined are often necessary to ensure an appropriate treatment, while prosthodontic treatment may be a challenge due to the skeletal discrepancies of the maxilla and the mandible. This clinical report presents a 45-year-old patient with a diagnosis of Angle Class II division 1 malocclusion. After clinical and radiographic observation and consultation with an orthodontist and maxillofacial surgeon, a combination of surgical and orthodontic therapy was proposed. Considering that the patient refused the surgical procedure due to fear, orthodontic therapy was carried out first, which improved the aesthetic and functional characteristics, but did not give results in increasing the vertical dimension of the occlusion. Therefore, further therapy was prosthetic, and was carried out by making all-ceramic maxillary and mandibular fixed dental prostheses; the patient was successfully rehabilitated and the anteroposterior discrepancy between the dental arches was corrected. Combined orthodontic and prosthetic treatment can be a good option in the complex treatment of patients with skeletal class II division 1 malocclusion providing functional rehabilitation of the stomatognathic system and improving facial appearance and the quality of life of the patient.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139230039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detrimental Effects of the COVID-19 Pandemic on Elective Orthopedic Surgery and Institutional Bone Bank Activity","authors":"S. Zeljko, Ristic M. Branko, Matic V Aleksandar","doi":"10.2478/sjecr-2021-0060","DOIUrl":"https://doi.org/10.2478/sjecr-2021-0060","url":null,"abstract":"Abstract The novel coronavirus SARS-CoV-2 (COVID-19) generated a 2020 global pandemic that has provoked drastic effects across world economies and healthcare systems. This article aimed to describe the impact of the COVID-19 pandemic on elective arthroplasty procedures and bone bank activity in the university hospital. We have performed a retrospective cohort study involving 600 adult orthopedic inpatients from March 15th to December 31st, 2019 and 2020, respectively. The number of primary total hip and knee arthroplasty procedures showed a highly significant decrease from 315 and 165 in the same period of 2019 to 106 and 14 in 2020 (p < 0.001). There was a highly significant decrease in the overall number of procurement procedures during the pandemic compared to the same period in 2019 (p < 0.001). The overall discard rate of bone allografts during the COVID-19 pandemic significantly increased from 31.57%. to 80.76% (p < 0.03). The leading cause of allograft rejection during 2019 and 2020 was the inability to perform serological tests due to lack of institutional support or the refusal of donors to perform a six-month retest. The COVID-19 pandemic had an extremely detrimental effect on elective orthopedic surgery and the normal functioning of the institutional bone bank.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139233817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Respiratory Rehabilitation Improves Quality of Life and Functionality of Covid 19 Patients","authors":"Vladan Marković, Aleksandra Jurišić-Škevin, V. Grbovic, S. Simovic, Ž. Todorovic, N. Zdravković, J. Dimitrijevic, N. Zdravkovic-Petrovic","doi":"10.2478/eabr-2023-0002","DOIUrl":"https://doi.org/10.2478/eabr-2023-0002","url":null,"abstract":"Abstract Respiratory rehabilitation leads to reduction of symptoms, strengthens extremity musculature and improves emotional state and management of daily activities in patients with respiratory diseases. Aim of our study was to determine quality of life and functionality of COVID 19 patients before and after respiratory rehabilitation program. The study was conducted at the Clinical Center, Kragujevac, from June to July 2020. The study was a prospective clinical trial and included 62 patients with the acutephase of COVID-19. Respiratory rehabilitation program started at hospital and continued at home for three months overall. Quality of life was measured by the EQ-5D-5L and patient’s functionality by The FIM score. All five dimension of EQ-5D-5L were higher after respiratory rehabilitation program as well as EQ-5D index score and VAS score (0.8516±0.202 and 53.31±17.129 before rehabilitation, 0.9147±0.074 and 64.53±8.368 after rehabilitation). Respiratory exercise showed significantly improvement in FIM total score from 104.48±12.880 to 106.21±9.791, as well as in FIM motor and cognitive subscores. Respiratory rehabilitation program improves quality of life and functionality of COVID 19 patients.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"304 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115439155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulatory Role of Galectin-1 in Ulcerative Colitis with Comorbid Metabolic Syndrome","authors":"Kemal Corovic, B. Stojanovic, Andjela Petrović, Isidora Stanisavljević, V. Marić, N. Zdravković, Marina Z Jovanovic","doi":"10.2478/eabr-2023-0006","DOIUrl":"https://doi.org/10.2478/eabr-2023-0006","url":null,"abstract":"Abstract Metabolic syndrome (MetS) and ulcerative colitis (UC) are widespread health conditions characterized by chronic, low-grade inflammation. Galectin-1 (Gal-1), an immunomodulatory peptide mainly secreted from adipose tissue, could potentially play a crucial role in mitigating these conditions. This cross-sectional study explores the involvement of Gal-1 in MetS and UC within a cohort of 75 patients, newly diagnosed with UC. The MetS subgroup displayed increased fecal Gal-1 levels compared to those without MetS. Furthermore, Gal-1 showed predominance over pro-inflammatory cytokines, including TNF-α, IL-6, and IL-17, in these subjects. These findings emphasize the potential involvement of Gal-1 in the pathophysiology of UC and MetS, presenting it as a promising diagnostic biomarker and therapeutic target for these conditions.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133883766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PD-1 Blockage Facilitates Cytotoxic T and NK Cells Tumoricidal Phenotype in a Murine Breast Carcinoma","authors":"Sanja Tripković, M. Jocic, Isidora Stanisavljević, Marina Z Jovanovic, M. Jurišević, Andjela Petrović, Milan M Jovanović, B. Milev, V. Marić, Marina Z Jovanovic","doi":"10.2478/eabr-2023-0005","DOIUrl":"https://doi.org/10.2478/eabr-2023-0005","url":null,"abstract":"Abstract In breast cancer therapy, as the leading cause of death in women, besides chemo-radiotherapy, immunotherapy has been increasingly used. PD-1/PD-L1 axis blockade primarily acts on T lymphocytes, the main effectors of acquired immune response. NK cells, which are part of the innate immune response, also play a role in the anti-tumor response through the blockade of this signaling pathway. The study was conducted to examine the effects of anti-PD-1 therapy on NK and T cells in mouse breast cancer. Female BALB/c mice were used, divided into two groups, one with induced breast cancer and one treated with anti-PD-1 antibody. Breast cancer cell line was used to induce the cancer, and the anti-PD-1 antibody was applied intraperitoneally. Cell populations in spleen and tumor microenvironment were examined using flow cytometry. Data were statistically analyzed using SPSS. The percentage of NK cells expressing FasL, NKG2D, and IFN-γ is significantly higher in spleen and tumor-infiltrating NK cells upon anti-PD-1 therapy, while the expression of inhibitory markers Foxp3 and IL-10 in regulatory NK cells is significantly lower. The percentage of T lymphocytes expressing CD107a and IL-17 is significantly higher in the spleen, while a higher number of T lymphocytes expressing CD69 is present in the tumor microenvironment. The study suggests that anti-PD-1 therapy can activate NK and T cells, and improve anti-tumor immune response in breast cancer. Further research is needed to understand the interplay between these cells during PD-1 blockage.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"0 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130256051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective Laser Trabeculoplasty-What Do We Know So Far? Review","authors":"Rada Zečevic, Sunčica B Srećković, D. Ristic, Anđelka Djuric, M. Kontić","doi":"10.2478/sjecr-2022-0032","DOIUrl":"https://doi.org/10.2478/sjecr-2022-0032","url":null,"abstract":"Abstract Selective laser trabeculoplasty (SLT) has been used for lowering intraocular pressure (IOP) in patients with different types of glaucoma. It is a safe and effective procedure as initial or additional therapy when target IOP could not be achieved with medical therapy. SLT could specifically target pigmented cells in trabecular meshwork (TM) leaving nonpigmented cells unaffected, which may allow repeat treatments and thus has lower complication rate. It can be successfully used for IOP reduction in primary open angle glaucoma (POAG), normotensive glaucoma (NTG), pigmentary glaucoma (PG), pseudoexfoliation glaucoma (PXFG), primary angle closure glaucoma (PACG), silicone-oil-induced glaucoma, and high IOP after failed trabeculectomy. SLT could also be used in all stages of open agle glaucoma but failure of SLT in advanced glaucoma patients should go to immediate filtering surgery to avoid postoperative fibrosis. SLT reduces IOP fluctuation. IOP-lowering effect of SLT diminishes over time, but it can be repeated in order to achieve additional or recurrent IOP reduction. Higher baseline IOP up to a certain level is the strongest predictor of success after SLT.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133812084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing the Effectiveness of Laser Therapy, Medicaments and Surgical Treatment in Patients with Angle Closed Glaucoma","authors":"Svetlana S. Paunović, Mirjana A. Janicijevic Petrovic, Sunčica B Srećković, M. Paunović, Katarina M. Janicijevic, Zora Stankovic","doi":"10.2478/sjecr-2021-0050","DOIUrl":"https://doi.org/10.2478/sjecr-2021-0050","url":null,"abstract":"Abstract The study goal was to compare the efficiency of the medicaments, laser and surgical treatment in the patients with primary angle closure glaucoma (PACG) in order to achieve: normalization of intraocular pressure (IOP), maintaining the useful visual acuity and stabilization of visual field loss. All patients were treated at Clinic of Ophthalmology of Clinical Centre Kragujevac in Kragujevac, Serbia, in the period from June 15, 2010 to June 15, 2014. There were 116 patients in this study. They diagnosed with PACG, and they had been adequate selected for this study. They were treated with: medicaments; Nd:YAG (Neodymium:Yittrium Aluminum Garnet) laser iridotomy, and glaucoma surgery. Clinical ophthalmology controls have been introduced once or twice a month, and the vision field loss was tested three times every year. During the monitoring period of one year: no statistically significant difference occurred in terms of changes of the visual acuity among the three study-groups. The best IOP regulation was achieved after the laser treatment (53.4%), followed by the surgical treatment (28.5%), while the weakest was recorded in patients treated with the medicaments (18.1%). The percentage of the visual field loss was the biggest of patients treated with the medicaments (57%), and then the patients treated with the laser iridotomy (35%), while the least one occurred of the patients with the surgical treatment (23%). In the laser-treated group of 62 patients, the frequency of complications was 17.8%; while the out of 33 patients who had been treated (surgical), the frequency of complications was 19%. Laser iridotomy was shown to be effective of 89.5% of study-patients with PACG, while non-reactive studypatients underwent to the trabeculectomy. In addition to the high efficacy of Nd:YAG laser iridotomy in regulating of IOP values of the patients with PACG, the advantage of laser-method was in: the maneuvering of outpatients, it was easy to do, had a short lead time and was used in local anesthesia.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"125 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131498029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subglottic Tracheal Stenosis, Resection, And Reconstruction: A Case Report","authors":"Branko Čampar, W. Klepetko","doi":"10.2478/sjecr-2021-0063","DOIUrl":"https://doi.org/10.2478/sjecr-2021-0063","url":null,"abstract":"Abstract Post-intubation stenosis are the most frequent indications for tracheal resection and reconstructions. They are mostly caused postintubation inflated cuff and after distal tracheostomy. 16- year-old female was admitted to thoracic surgery department, General hospital Vienna with the diagnosis of an impossible weaning with a tracheostomy in place. The pre-operative bronchoscopy and MSCT of the neck evaluation revealed a total occlusion of the trachea below the cricoid arch and reaching distally to the level of the tracheostomy (total length approx. 3cm) by means of an acquired tracheostomy-associated tracheal stenosis (Myer-Cotton IV°). The distal trachea was unaffected. Thus, the indication for a surgical repair was set. Tracheal resection through a cervical incision was performed. The pre-existing tracheostomy as well as the stenotic segment was resected (resection length approx. 3.5cm) and a cricotracheal end-to-end anastomosis was performed. Subglottic resection of the trachea is rare, if conducted a good selection of patients performed precise surgical procedures with the support of anesthesia is considered by some to be the procedure of choice for the treatment severe (>70% luminal obstruction).","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"362 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133068302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}