亚致死剂量急性总伽马辐射对红细胞生物物理特性、脂质过氧化、抗氧化供应和红细胞凝血特性的影响

T. Zaporozhets, L. Korovina, O. Sanyk
{"title":"亚致死剂量急性总伽马辐射对红细胞生物物理特性、脂质过氧化、抗氧化供应和红细胞凝血特性的影响","authors":"T. Zaporozhets, L. Korovina, O. Sanyk","doi":"10.2478/sjecr-2022-0048","DOIUrl":null,"url":null,"abstract":"Abstract The aim of the investigation was to study the effect of acute, total gamma-irradiation in a sublethal dose on the biophysical properties of erythrocytes, the intensity of lipid peroxidation, antioxidant supply and hemocoagulating properties of erythrocytes. The experiments were carried out on 11-12-week-old age guinea pigs, males and females in equal numbers. The animals were exposed to a single total radiation at a dose of 4.5 Gy (sublethal dose, LD 50/30). The studies were carried out on the 7th day after exposure to radiation (at the height of radiation sickness). The development of radiation damage was accompanied by intense erythropoiesis and the appearance of erythrocytes with a high resistance to hemolysis and an increased sedimentation rate. After acute gamma irradiation, depletion of the antioxidant system was noted. It manifested in a decrease in the activity of superoxide dismutase of erythrocytes by 19.7% (p<0.01) and the concentration of serum ceruloplasmin by 21.5% (p<0.01). The content of thiobarbituric acid reactive substances (TBARS) and their accumulation while the incubation of erythrocytes remained within the normal range. The erythrocytes of the irradiated animals exhibited increased procoagulant and decreased antiheparin activity, which reflects conformational changes in highly radiosensitive fatty acid chains of phospholipids in their membranes. A decrease in the fibrinolytic activity of erythrocytes in irradiated animals was found.","PeriodicalId":104738,"journal":{"name":"Experimental and Applied Biomedical Research (EABR)","volume":"17 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Effect of the Acute Total Gamma Radiation in a Sublethal Dose on the Biophysical Properties of Red Blood Cells, Lipid Peroxidation, Antioxidant Supply and Hemocoagulating Properties of Erythrocytes\",\"authors\":\"T. Zaporozhets, L. Korovina, O. Sanyk\",\"doi\":\"10.2478/sjecr-2022-0048\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract The aim of the investigation was to study the effect of acute, total gamma-irradiation in a sublethal dose on the biophysical properties of erythrocytes, the intensity of lipid peroxidation, antioxidant supply and hemocoagulating properties of erythrocytes. The experiments were carried out on 11-12-week-old age guinea pigs, males and females in equal numbers. The animals were exposed to a single total radiation at a dose of 4.5 Gy (sublethal dose, LD 50/30). The studies were carried out on the 7th day after exposure to radiation (at the height of radiation sickness). The development of radiation damage was accompanied by intense erythropoiesis and the appearance of erythrocytes with a high resistance to hemolysis and an increased sedimentation rate. After acute gamma irradiation, depletion of the antioxidant system was noted. It manifested in a decrease in the activity of superoxide dismutase of erythrocytes by 19.7% (p<0.01) and the concentration of serum ceruloplasmin by 21.5% (p<0.01). The content of thiobarbituric acid reactive substances (TBARS) and their accumulation while the incubation of erythrocytes remained within the normal range. The erythrocytes of the irradiated animals exhibited increased procoagulant and decreased antiheparin activity, which reflects conformational changes in highly radiosensitive fatty acid chains of phospholipids in their membranes. A decrease in the fibrinolytic activity of erythrocytes in irradiated animals was found.\",\"PeriodicalId\":104738,\"journal\":{\"name\":\"Experimental and Applied Biomedical Research (EABR)\",\"volume\":\"17 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-05-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental and Applied Biomedical Research (EABR)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2478/sjecr-2022-0048\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental and Applied Biomedical Research (EABR)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2478/sjecr-2022-0048","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

摘要

摘要本研究旨在探讨急性亚致死剂量全γ辐照对红细胞生物物理特性、脂质过氧化强度、抗氧化供应和红细胞凝血特性的影响。实验是在11-12周龄的豚鼠身上进行的,雄性和雌性的数量相等。这些动物接受单次总辐射,剂量为4.5 Gy(亚致死剂量,ld50 /30)。这些研究是在暴露于辐射后的第7天进行的(在辐射病的高峰期)。放射损伤的发展伴随着强烈的红细胞生成和红细胞对溶血的高抵抗和沉降率的增加。急性γ辐照后,发现抗氧化系统的损耗。红细胞超氧化物歧化酶活性降低19.7% (p<0.01),血清铜蓝蛋白浓度降低21.5% (p<0.01)。红细胞孵育时,硫代巴比妥酸反应性物质(TBARS)的含量及其积累均在正常范围内。受辐射动物的红细胞表现出促凝活性增加和抗肝素活性降低,这反映了其膜中高度辐射敏感的磷脂脂肪酸链的构象变化。发现辐照动物红细胞的纤溶活性降低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of the Acute Total Gamma Radiation in a Sublethal Dose on the Biophysical Properties of Red Blood Cells, Lipid Peroxidation, Antioxidant Supply and Hemocoagulating Properties of Erythrocytes
Abstract The aim of the investigation was to study the effect of acute, total gamma-irradiation in a sublethal dose on the biophysical properties of erythrocytes, the intensity of lipid peroxidation, antioxidant supply and hemocoagulating properties of erythrocytes. The experiments were carried out on 11-12-week-old age guinea pigs, males and females in equal numbers. The animals were exposed to a single total radiation at a dose of 4.5 Gy (sublethal dose, LD 50/30). The studies were carried out on the 7th day after exposure to radiation (at the height of radiation sickness). The development of radiation damage was accompanied by intense erythropoiesis and the appearance of erythrocytes with a high resistance to hemolysis and an increased sedimentation rate. After acute gamma irradiation, depletion of the antioxidant system was noted. It manifested in a decrease in the activity of superoxide dismutase of erythrocytes by 19.7% (p<0.01) and the concentration of serum ceruloplasmin by 21.5% (p<0.01). The content of thiobarbituric acid reactive substances (TBARS) and their accumulation while the incubation of erythrocytes remained within the normal range. The erythrocytes of the irradiated animals exhibited increased procoagulant and decreased antiheparin activity, which reflects conformational changes in highly radiosensitive fatty acid chains of phospholipids in their membranes. A decrease in the fibrinolytic activity of erythrocytes in irradiated animals was found.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
0.60
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信