Clinical, Cosmetic and Investigational Dentistry最新文献

{"title":"Impact of a Multi-Component Preventive Oral Health Program on Dental Plaque Scores for Individuals with Special Health Care Needs.","authors":"Wejdan Saad Alrazooq, Sultana A Alhurishi, Altaf H Shah","doi":"10.2147/CCIDE.S584289","DOIUrl":"10.2147/CCIDE.S584289","url":null,"abstract":"<p><strong>Purpose: </strong>Plaque accumulation is considered to be one of the main factors in various oral diseases. This study aimed to evaluate the effect of oral health counselling on plaque scores for Individuals with Special Health Care Needs (ISHCN) attending special care dentistry clinics.</p><p><strong>Patients and methods: </strong>A prospective observational study was designed and consecutive sampling was used among the adult patients attending special care dentistry clinics at a University Dental Hospital. A total of 120 participants (61 males and 59 females between) aged between 16 and 69 years were enrolled in the study. Participation was voluntary, and recruits were selected after the application of a set of inclusion and exclusion criteria. The participants were categorized according to their specific health care needs. The subjects were assessed for plaque scores using the Silness-Löe Plaque Index for a period of six months. The first observation was noted at baseline and then every two weeks until the next three months. Finally, monthly plaque scores were recorded for the subsequent three months. Ten observations were recorded for each participant. All data were entered and analyzed using Statistical Package for Social Sciences (SPSS v.22) software.</p><p><strong>Results: </strong>The majority of the sample was comprised of the \"Medically compromised group\", followed by the \"Intellectual disability\" group. The overall baseline mean plaque index score was 2.68, which reduced significantly (p = 0.01) at the completion of 6 months period.</p><p><strong>Conclusion: </strong>The multi-component preventive oral health program and repeated follow up was associated with significant reduction in dental plaque scores among individuals with special health care need over six months.</p>","PeriodicalId":10445,"journal":{"name":"Clinical, Cosmetic and Investigational Dentistry","volume":"18 ","pages":"584289"},"PeriodicalIF":1.8,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13332728/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148389724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of a Customized Milled Metal Surgical Guide for Esthetic Crown Lengthening: A Case Report.","authors":"Abdulkareem Alhumaidan, Sarah Fita","doi":"10.2147/CCIDE.S622098","DOIUrl":"10.2147/CCIDE.S622098","url":null,"abstract":"<p><strong>Purpose: </strong>Excessive gingival display (EGD) resulting from altered passive eruption (APE) is a clinical condition that is commonly managed by esthetic crown lengthening to achieve stable and symmetrical gingival margins. Determining the appropriate gingival and osseous resection levels remains a key step in achieving predictable surgical outcomes. Although digitally printed resin guides have been widely discussed, clinical data regarding milled metal dual-purpose guides remain limited.</p><p><strong>Patients and methods: </strong>This single case report (n=1) describes the digital workflow for designing and fabricating a dual-purpose cobalt-chromium surgical guide to assist both gingivectomy and osteoectomy. The guide dimensions were determined by superimposing cone-beam computed tomography (CBCT) data with intraoral scan files to identify the cementoenamel junction (CEJ). The guide was fabricated using a subtractive milling process and used during esthetic crown lengthening in a patient diagnosed with altered passive eruption type I.</p><p><strong>Results: </strong>The milled metal guide appeared to provide stable positioning during surgery and helped guide both soft- and hard-tissue resection. At 6-month follow-up, gingival margins remained stable, symmetrical contouring was achieved, patient satisfaction was high, and no immediate postoperative complications were observed.</p><p><strong>Conclusion: </strong>This report presents a CBCT-integrated workflow for dual-purpose surgical guidance. The rigidity of the milled metal framework may assist intraoperative stability and support favorable short-term clinical outcomes in this case.</p>","PeriodicalId":10445,"journal":{"name":"Clinical, Cosmetic and Investigational Dentistry","volume":"18 ","pages":"622098"},"PeriodicalIF":1.8,"publicationDate":"2026-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13310401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148343605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adapting the Chairside: Pediatric Dental Management for Children with Neurodevelopmental Disorders - A Scoping Review.","authors":"Putu Ayu Pradnya Swastiantari, Risti Saptarini Primarti, Iwan Ahmad M, Prima Andisetyanto, Naninda Berliana Pratidina","doi":"10.2147/CCIDE.S605432","DOIUrl":"10.2147/CCIDE.S605432","url":null,"abstract":"<p><strong>Background: </strong>Children with neurodevelopmental disorders (NDD), especially autism spectrum disorder (ASD), are commonly characterized by sensory sensitivities, communication differences, and anxiety during dental treatment. These factors can reduce cooperation and prevent completion of care. This scoping review mapped recent evidence on non-pharmacological chairside strategies used to support pediatric dental management for children with NDD.</p><p><strong>Methods: </strong>This scoping review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) guidelines. A systematic literature search was conducted in PubMed, Scopus, ScienceDirect, and SpringerLink for publications from 2020 to 2026. The search focused on non-pharmacological chairside behaviour management strategies for children aged 0-18 years with neurodevelopmental disorders in pediatric dental settings. Predefined keywords and the Population, Concept, and Context (PCC) framework were used to guide the search.</p><p><strong>Results: </strong>Six studies from Malaysia, California, China, Italy, Turkey, and Brazil were included. Most studies focused on autism spectrum disorder, with limited representation of other neurodevelopmental profiles. Five chairside strategy domains were mapped: Tell-Show-Do-based behavior guidance, desensitization often supported by video or point-of-view modeling, structured visual pedagogy, sensory-adapted dental environments, and protective stabilization or passive immobilization. Reported outcomes were heterogeneous and included cooperation ratings, procedural tolerance, observed distress, and physiological markers.</p><p><strong>Conclusion: </strong>Non-pharmacological chairside strategies for children with NDD show potential, particularly structured preparation, visual supports, and sensory-adapted environments. However, the evidence remains limited and methodologically heterogeneous. Standardized child-centered outcome sets and broader inclusion of non-ASD NDD subgroups are needed.</p>","PeriodicalId":10445,"journal":{"name":"Clinical, Cosmetic and Investigational Dentistry","volume":"18 ","pages":"605432"},"PeriodicalIF":1.8,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13281862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148293634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Modified Expandable Headgear-Activator for Combined Adenotonsillar Hypertrophy and Bimaxillary Constriction: Insights from a 9-Year Follow-Up.","authors":"Tao Qiu, Mouyuan Sun, Yaxian Luo, Tinglu Fang, Xiangtao Liu","doi":"10.2147/CCIDE.S610362","DOIUrl":"10.2147/CCIDE.S610362","url":null,"abstract":"<p><p>This report describes the successful long-term, non-extraction orthodontic management of an adolescent with high-angle skeletal Class II malocclusion, bimaxillary constriction, lip incompetence, and chronic mouth breathing with adenotonsillar hypertrophy. Treatment initiated at CVMS II employed a patented headgear-activator appliance to achieve concurrent vertical control, mandibular advancement, and arch expansion, followed by fixed therapy. Five-year clinical follow-up with cephalometric evaluation at the end of active treatment confirmed significant skeletal correction, with the ANB angle reducing from 4.4° to 3.0°, while vertical dimensions remained stable (SN-MP: 41.8° to 41.3°; FH-MP: 34.3° to 32.2°). Transverse expansion resolved the posterior crossbite, transforming constricted arches into harmonious ovoid forms. Lip seal and a straight facial profile were achieved without surgical airway intervention. These outcomes suggest that early functional orthopedic treatment emphasizing three-dimensional control prior to fixed therapy can yield stable skeletal, occlusal, and aesthetic results while circumventing extractions. The prolonged soft tissue adaptation observed further underscores the necessity of extended follow-up for accurate assessment of orthopedic stability.</p>","PeriodicalId":10445,"journal":{"name":"Clinical, Cosmetic and Investigational Dentistry","volume":"18 ","pages":"610362"},"PeriodicalIF":1.8,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13264402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148249539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regenerative Potential of Exosomes from 3D Spheroid-Cultured Stem Cells Compared with 2D Culture in Dentin-Pulp Regeneration: A Systematic Review.","authors":"Hesti Witasari Jos Erry, Anggraini Margono, Dini Asrianti Bagio, Dewi Fatma Suniarti, Indra Kusuma, Hendrik Setia Budi, Rachmat Mauludin, Rahmi Syaflida Dalimunthe, Dodi Valentino Tambun, Lisa Rinanda Amir","doi":"10.2147/CCIDE.S614885","DOIUrl":"10.2147/CCIDE.S614885","url":null,"abstract":"<p><strong>Purpose: </strong>This systematic review aimed to evaluate the current preclinical evidence on exosomes derived from three-dimensional stem cell culture systems and their direct or indirect relevance to dentin-pulp regeneration. Exosomes derived from stem cells have emerged as promising mediators of tissue regeneration because of their ability to transfer bioactive molecules that regulate cellular communication and tissue repair. Recent studies suggest that exosomes obtained from three-dimensional (3D) spheroid cultures may exhibit enhanced regenerative properties compared with those derived from conventional two-dimensional cultures.</p><p><strong>Methods: </strong>A systematic review was conducted following the PRISMA 2020 guidelines. Electronic searches were performed in PubMed, Scopus, and ProQuest databases to identify relevant studies investigating exosomes derived from 3D cell culture systems with potential relevance to dentin-pulp regeneration. Studies were screened according to predefined inclusion and exclusion criteria. Data extraction focused on cell sources, exosome isolation methods, characterization techniques, and biological effects related to dental pulp regeneration.</p><p><strong>Results: </strong>Ten studies met the inclusion criteria. The included studies demonstrated that exosomes derived from 3D spheroid cultures may enhance angiogenesis, support odontogenic differentiation, reduce inflammatory responses, and promote regenerative tissue repair. Several studies also reported increased expression of regenerative markers and improved cellular proliferation when compared with exosomes derived from conventional two-dimensional cultures. However, only a limited number of studies directly investigated dentin-pulp-related outcomes, while several provided indirect mechanistic evidence from non-dental preclinical models.</p><p><strong>Conclusion: </strong>Exosomes derived from three-dimensional culture systems show promising potential as a cell-free therapeutic strategy for dentin-pulp regeneration. These vesicles may improve tissue repair through enhanced biological activity. However, the current evidence remains mainly preclinical, heterogeneous, and partly indirect. Further standardized experimental studies and clinical investigations are required to validate their translational application in regenerative endodontic therapy.</p>","PeriodicalId":10445,"journal":{"name":"Clinical, Cosmetic and Investigational Dentistry","volume":"18 ","pages":"614885"},"PeriodicalIF":1.8,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13242206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148197461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and Microbiological Changes Following Multi-Strain Probiotic Mouthrinse Use in Dental Students with Halitosis: A Prospective Single-Arm Study.","authors":"Khadija Muhamed Ahmed","doi":"10.2147/CCIDE.S609391","DOIUrl":"10.2147/CCIDE.S609391","url":null,"abstract":"<p><strong>Background: </strong>Halitosis is a common oral health condition that negatively affects social interactions, psychological well-being, and professional confidence, particularly among dental students. Conventional antimicrobial mouthwashes, although effective, are associated with adverse effects and disruption of the oral microbiota. Probiotics have emerged as a promising alternative for modulating microbial communities and enhancing oral ecological balance. Quantitative real-time polymerase chain reaction (qPCR) enables detailed assessment of microbiological changes associated with halitosis.</p><p><strong>Aim: </strong>To assess the clinical and microbiological changes associated with a multi-strain probiotic mouthrinse containing <i>Lactobacillus reuteri</i> and <i>Lactobacillus paracasei</i> in dental students with halitosis using qPCR.</p><p><strong>Materials and methods: </strong>This prospective single-arm study included dental students with clinically confirmed halitosis (organoleptic test [OLT] score ≥2). Participants used the probiotic mouthrinse three to four times daily for 14 days. Clinical parameters, including OLT, tongue-coating area (Ta), tongue-coating thickness (Tt), and total tongue-coating score (TCS), were assessed at baseline, day 7, and day 14. Stimulated whole saliva samples were collected at each time point and analyzed using qPCR to assess relative changes in <i>Streptococcus mutans, Lactobacillus salivarius</i>, and <i>Porphyromonas gingivalis</i>.</p><p><strong>Results: </strong>Thirty-four participants completed the study. Significant reductions were observed in OLT, Ta, Tt, and TCS over the 14-day period (p < 0.001). qPCR analysis demonstrated reduced abundance of <i>S. mutans</i> and <i>L. salivarius</i>, with a modest decrease in <i>P. gingivalis</i>. Improvements in OLT were significantly associated with changes in bacterial profiles, particularly <i>S. mutans</i> and <i>P. gingivalis</i> (p < 0.001). A strong positive correlation was consistently observed between TCS and OLT at all time points (p < 0.001).</p><p><strong>Conclusion: </strong>Within the limitations of this study, the probiotic mouthrinse was associated with improvements in clinical indicators of halitosis and favorable shifts in selected oral microbiota. These findings support further investigation through well-designed randomized controlled trials.</p>","PeriodicalId":10445,"journal":{"name":"Clinical, Cosmetic and Investigational Dentistry","volume":"18 ","pages":"609391"},"PeriodicalIF":1.8,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13222015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148136840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Postoperative Instruction Delivery Methods on Patient Compliance After Minor Oral Surgeries: A Systematic Review.","authors":"Ahmed Khalil Fawzy, Ahmed Hossam Soliman, Mohamed Fakhry Hussein, Wesal Youssef Hassan, Marwa Mostafa Mohamed, Hossam Mohamed Hassan Soliman","doi":"10.2147/CCIDE.S616315","DOIUrl":"10.2147/CCIDE.S616315","url":null,"abstract":"<p><strong>Background: </strong>It has been demonstrated that giving precise postoperative instructions reduces the possibility of adverse events after surgery. Adherence to postoperative instructions during recovery is impacted by patient comprehension, which is influenced by the approach of instruction delivery. This systematic review's main goal was to critically assess the available data regarding the association between the methods of introducing postoperative instructions and patients' compliance after minor oral surgeries.</p><p><strong>Methods: </strong>In accordance with the Cochrane Handbook for Systematic Reviews of Interventions, a systematic review was conducted. Four databases (PubMed, Web of Science (WOS), Scopus, and Google Scholar) were thoroughly searched up to October 31, 2025. Covidence software was used by two independent reviewers to screen the material. The methodological quality and risk of bias in included studies were systematically assessed using the Quality Assessment Tool in Covidence. Ten studies were included: seven Randomized Controlled Trials, one cross-sectional, and two cohort studies.</p><p><strong>Results: </strong>Two studies reported a statistically significant positive association between phone call follow-up and adherence to postoperative instructions, showing that phone follow-up had higher scores of compliance level (9.7±0.5 and 9.14±0.78) compared to extended written instructions (7.9±2.1) and to verbal and printed instructions combined (7.64±0.83), respectively. Pictorial instructions or Android-based mobile applications were found to significantly improve patient compliance in two other research studies. Another study demonstrated that audiovisual (A-V) aids for postoperative instructions had a higher median comprehension score (12±2) than the verbal (9.5±3) and written (10±2) methods. However, findings from three studies on verbal and written instructions were inconsistent.</p><p><strong>Conclusion: </strong>The use of enhancement techniques of postoperative instruction, such as multimedia, mobile applications, phone calls, and pictorial instructions, particularly when combined with baseline verbal explanations, demonstrated superior efficacy in enhancing patient comprehension, information retention, and compliance across diverse patient populations who had undergone minor oral surgeries.</p>","PeriodicalId":10445,"journal":{"name":"Clinical, Cosmetic and Investigational Dentistry","volume":"18 ","pages":"616315"},"PeriodicalIF":1.8,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13222047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148136836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral Radiographic Changes in Severe Sickle Cell Anemia Patients: A Retrospective Comparative Study.","authors":"Haifa AlKhodier, Fahad Bin Saleh, Abdulmajeed AlRumi, Tariq AlOjaym","doi":"10.2147/CCIDE.S611537","DOIUrl":"10.2147/CCIDE.S611537","url":null,"abstract":"<p><strong>Introduction: </strong>Sickle cell disease is caused by a point mutation in the β-globin gene, resulting in the production of abnormal hemoglobin. Various oral radiographic changes have been documented in the literature, though they are less frequently reported in patients with severe disease. This study aimed to evaluate specific radiographic features in pediatric patients with severe sickle cell disease.</p><p><strong>Methods: </strong>This retrospective comparative study was conducted at the Dental Department of King Faisal Specialist Hospital and Research Centre. A total of 72 pediatric patients (aged 6-14 years) with genetically confirmed severe sickle cell disease and 96 age-matched healthy controls were included. Dental radiographs were evaluated by four calibrated examiners for the presence of radiopaque areas, increased spacing of bony trabeculae, absence of mandibular canal corticalization, faint lamina dura, spiky roots, taurodontism, and haziness of the maxillary sinuses. Statistical analysis was performed using Chi-square test, with significance set at p < 0.05.</p><p><strong>Results: </strong>The sickle cell group showed a significantly higher prevalence of increased trabecular spacing (58.3% vs. 19.8%), absence of mandibular canal corticalization (68.1% vs. 16.7%), faint lamina dura (19.4% vs. 2.1%), taurodontism (15.3% vs. 9.4%), and haziness of the maxillary sinuses (69.4% vs. 43.8%) (p < 0.05 for all comparisons).</p><p><strong>Conclusion: </strong>Pediatric patients with severe sickle cell disease demonstrated a higher prevalence of specific radiographic changes, particularly increased trabecular spacing, loss of mandibular canal corticalization, and haziness of the maxillary sinuses. This study highlights the importance of recognizing these imaging features in the assessment of disease severity.</p>","PeriodicalId":10445,"journal":{"name":"Clinical, Cosmetic and Investigational Dentistry","volume":"18 ","pages":"611537"},"PeriodicalIF":1.8,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13209138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148041387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Association of Mandibular Three-Rooted Deciduous Second and Permanent First Molars: A Retrospective CBCT Study in Iraqi Pediatric Population.","authors":"Ranjdar Talabani, Zhwan Jamal Rashid, Arass Noori, Anwar Amin, Hawzhen Masoud Mohammed Saeed","doi":"10.2147/CCIDE.S607617","DOIUrl":"10.2147/CCIDE.S607617","url":null,"abstract":"<p><strong>Purpose: </strong>To estimate the prevalence of three-rooted mandibular deciduous second molars (3RME) and three-rooted mandibular permanent first molars (3RM6) in Iraqi children and to evaluate their association using cone-beam computed tomography (CBCT).</p><p><strong>Methods: </strong>This retrospective cross-sectional study analyzed CBCT scans of Iraqi children aged 7-12 years (N = 196). Root morphology was assessed in axial, coronal, and sagittal planes. Prevalence was calculated at the patient level (presence of ≥1 affected tooth). Associations between 3RME and 3RM6 were tested using the chi-square test, and age/sex were explored as predictors using logistic regression. Intra- and inter-examiner reliability were assessed with Cohen's kappa.</p><p><strong>Results: </strong>Three-rooted morphology was identified in 3/196 children for 3RME (1.53%; 95% CI 0.52-4.40) and 10/196 children for 3RM6 (5.10%; 95% CI 2.80-9.14). A significant association was observed between 3RME and 3RM6 (χ²(4, N = 196) = 96.48, p < 0.001; Cramér's V = 0.50). Age and sex were not significant predictors (p > 0.05).</p><p><strong>Conclusion: </strong>Three-rooted mandibular molars were uncommon in this Iraqi pediatric sample; however, 3RME co-occurred significantly with 3RM6, indicating a strong association that may be clinically useful for anticipatory diagnosis and treatment planning.</p>","PeriodicalId":10445,"journal":{"name":"Clinical, Cosmetic and Investigational Dentistry","volume":"18 ","pages":"607617"},"PeriodicalIF":1.8,"publicationDate":"2026-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13180699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147970822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticle-Assisted Endodontic Irrigation: A Systematic Review of Preclinical Studies.","authors":"Hashim Mueen Hussein, Ahmed Hamid Ali","doi":"10.2147/CCIDE.S610759","DOIUrl":"10.2147/CCIDE.S610759","url":null,"abstract":"<p><strong>Background: </strong>Conventional root canal irrigants such as sodium hypochlorite (NaOCl) and ethylenediaminetetraacetic acid (EDTA) are widely used because of their antimicrobial and chelating properties; however, their effectiveness may be limited by biofilm resistance and complex root canal anatomy, and they may adversely affect dentin microstructure and regenerative potential.</p><p><strong>Objective: </strong>This systematic review evaluated preclinical evidence on nanoparticle-based endodontic irrigation with respect to antimicrobial and antibiofilm activity, dentin-related outcomes, and biocompatibility or regeneration-related effects.</p><p><strong>Methods: </strong>PubMed/MEDLINE and ScienceDirect were systematically searched for English-language studies published from January 2015 to December 2025 using the following Boolean strategy: (\"Nanoparticles\" OR \"Nano-based\") AND (\"Root canal irrigant\" OR \"Antibiofilm\") AND (\"Dentin surface\" OR \"Biocompatibility\"). Eligible studies were original preclinical investigations evaluating nanoparticle-assisted irrigation or disinfection in extracted human-tooth models. After screening 695 records against predefined eligibility criteria, 12 studies were included.</p><p><strong>Results: </strong>Compared with conventional irrigants, nanoparticle-based systems showed promising antibacterial and antibiofilm effects, particularly against <i>Enterococcus faecalis</i>. Activation approaches, including photodynamic, sonic, and ultrasonic methods, were frequently associated with enhanced antimicrobial performance. Some studies also reported improved smear-layer removal, preservation of dentin ultrastructure, and favorable mechanical outcomes. In addition, several biology-related studies suggested acceptable cytocompatibility, growth-factor release, and improved healing-related responses under experimental conditions.</p><p><strong>Conclusion: </strong>Nanoparticle-assisted irrigation shows promising preclinical potential as an adjunct to conventional root canal disinfection. However, heterogeneity in study design and the absence of clinical evidence limit definitive conclusions and preclude routine clinical recommendations at present.</p>","PeriodicalId":10445,"journal":{"name":"Clinical, Cosmetic and Investigational Dentistry","volume":"18 ","pages":"610759"},"PeriodicalIF":1.8,"publicationDate":"2026-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13180361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147970850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}