Clinics and research in hepatology and gastroenterology最新文献_第8页

Methylation profile of CD247 and FOXP3 genes and frequency of certain HLA-DQ haplotypes in Celiac disease 乳糜泻中CD247和FOXP3基因的甲基化谱和某些HLA-DQ单倍型的频率

IF 2.6 4区医学

Clinics and research in hepatology and gastroenterology Pub Date : 2025-02-24 DOI: 10.1016/j.clinre.2025.102562

Malik Ejder Yildirim , Hilmi Ataseven , Hande Kucuk Kurtulgan , Seyma Tastemur , Ahmet Sirin

{"title":"Methylation profile of CD247 and FOXP3 genes and frequency of certain HLA-DQ haplotypes in Celiac disease","authors":"Malik Ejder Yildirim , Hilmi Ataseven , Hande Kucuk Kurtulgan , Seyma Tastemur , Ahmet Sirin","doi":"10.1016/j.clinre.2025.102562","DOIUrl":"10.1016/j.clinre.2025.102562","url":null,"abstract":"<div><h3>Background</h3><div>Celiac disease is an autoimmune disorder that affects the small intestine in people with gluten intolerance. HLA-DQ2 and DQ8 have been associated with Celiac Disease. CD247 is a subunit of the T cell receptor complex and Forkhead box P3 (FOXP3) is a transcription factor involved in the regulation of the immune response. Expression levels of these two markers in various diseases, including autoimmune disorders, are controversial. In this context, we aimed to shed light on the etiopathogenesis of Celiac disease by determining the methylation profile of <em>CD247</em> and <em>FOXP3</em> genes, and to calculate the frequency of HLA-DQ haplotypes in this disease.</div></div><div><h3>Methods</h3><div>Methylated and unmethylated copy numbers of the <em>CD247</em> and <em>FOXP3</em> genes in samples were calculated using the methylation-specific qPCR method. The records regarding HLA-DQ2 and DQ8 genotypes previously detected from our patients by Real Time PCR and tissue transglutaminase IgA (TTG-IgA) by ELISA, were analyzed.</div></div><div><h3>Results</h3><div><em>CD247</em> methylation rate in our patients was significantly lower than in controls. According to the Marsh classification, the methylation level in Marsh type 2–3 patients was statistically lower than in type 1. On the contrary, <em>FOXP3</em> had a significantly higher methylation rate in the patient group compared to healthy controls, and this gene was also found to be more methylated in Marsh type 2–3 patients than in Marsh type 1. In the patient group, HLA-DQ2 positivity was 82.5% and HLA-DQ8 positivity was 37.5%.</div></div><div><h3>Conclusion</h3><div>The data suggest that CD247 expression is upregulated, whereas FOXP3 expression is downregulated in Celiac disease. Among HLA haplotypes, HLA-DQ2 heterodimer came to the forefront with its frequency in terms of celiac predisposition.</div></div>","PeriodicalId":10424,"journal":{"name":"Clinics and research in hepatology and gastroenterology","volume":"49 4","pages":"Article 102562"},"PeriodicalIF":2.6,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements of direct oral anticoagulants in cirrhotic individuals with portal vein thrombosis 直接口服抗凝剂治疗肝硬化合并门静脉血栓的进展。

IF 2.6 4区医学

Clinics and research in hepatology and gastroenterology Pub Date : 2025-02-19 DOI: 10.1016/j.clinre.2025.102553

Zhiqian Liu , Xiying Yang, Haitao Jiang, Rui Xie, Hong Wang

引用次数: 0

A real-world study on the characteristics of autoimmune gastritis: A single-center retrospective cohort in China 自身免疫性胃炎特征的真实世界研究：中国单中心回顾性队列研究

IF 2.6 4区医学

Clinics and research in hepatology and gastroenterology Pub Date : 2025-02-15 DOI: 10.1016/j.clinre.2025.102556

Yu Chen , Xiaowei Ji , Weiyi Zhao , Jie Lin , Siyuan Xie , Jinghong Xu , Jianshan Mao

{"title":"A real-world study on the characteristics of autoimmune gastritis: A single-center retrospective cohort in China","authors":"Yu Chen , Xiaowei Ji , Weiyi Zhao , Jie Lin , Siyuan Xie , Jinghong Xu , Jianshan Mao","doi":"10.1016/j.clinre.2025.102556","DOIUrl":"10.1016/j.clinre.2025.102556","url":null,"abstract":"<div><h3>Background and Aim</h3><div>Autoimmune gastritis (AIG) was previously considered a rare disease in China, and its clinical characteristics were not fully understood. This study aimed to demonstrate the characteristics of AIG in China and evaluate gastric oxyntic mucosal atrophy using a modified AIG-atrophic staging.</div></div><div><h3>Methods</h3><div>This was a single-center retrospective observational real-world study. The diagnosis of AIG was based on pathological results combined with parietal cell antibody (PCA) and intrinsic factor antibody (IFA) results, and endoscopic findings.</div></div><div><h3>Results</h3><div>A total of 745 patients were enrolled, the median age at diagnosis was 58 years old, and 69.9 % were female. The symptoms of AIG patients were nonspecific, and about 2/5 of the cases were asymptomatic. The proportions of cases from modified AIG-atrophic stage 1 to 4 were 0.8 %, 14.1 %, 73.8 %, and 11.3 %, respectively. Approximately 1/5 had autoimmune thyroiditis (AITD). Near 1/2 had one or more comorbidities: iron-deficiency anemia (IDA), pernicious anemia (PA), neuropathy, gastric hyperplastic polyps (GHP), gastric intraepithelial neoplasia (GIN), type 1 gastric neuroendocrine tumors (g-NET), or gastric adenocarcinoma (GAC). There was a high risk of type 1 g-NET (7.0 %) and GAC (9.1 %) in AIG patients.</div></div><div><h3>Conclusions</h3><div>AIG is not rare in China, and its early diagnosis is challenging, accompanied by a high risk of GAC. The modified four-stage AIG-atrophic staging can effectively represent the extent of oxyntic mucosal atrophy and the progression in AIG.</div></div>","PeriodicalId":10424,"journal":{"name":"Clinics and research in hepatology and gastroenterology","volume":"49 4","pages":"Article 102556"},"PeriodicalIF":2.6,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143437178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suboptimal use of inpatient palliative care consultation in alcoholic hepatitis hospitalizations may lead to higher readmissions 酒精性肝炎住院患者姑息治疗咨询不理想可能导致更高的再入院率。

IF 2.6 4区医学

Clinics and research in hepatology and gastroenterology Pub Date : 2025-02-11 DOI: 10.1016/j.clinre.2025.102554

Ali Jaan , Syeda Shahnoor , Abdul Moiz Khan , Umer Farooq , Qurat Ul Ain Muhammad , Kamran Qureshi

{"title":"Suboptimal use of inpatient palliative care consultation in alcoholic hepatitis hospitalizations may lead to higher readmissions","authors":"Ali Jaan , Syeda Shahnoor , Abdul Moiz Khan , Umer Farooq , Qurat Ul Ain Muhammad , Kamran Qureshi","doi":"10.1016/j.clinre.2025.102554","DOIUrl":"10.1016/j.clinre.2025.102554","url":null,"abstract":"<div><h3>Aims</h3><div>Alcoholic hepatitis (AH), a clinical syndrome precipitated by chronic alcohol consumption, constitutes about 0.9 % of total admissions in the United States. It presents a wide severity spectrum, from mild disease to severe cases associated with high mortality. Palliative care (PC) is a specialized medical approach focused on enhancing the quality of life for patients with serious or life-threatening illnesses. This study aims to investigate the national trends of palliative care (PC) utilization in AH hospitalizations and its impact on resource utilization.</div></div><div><h3>Methods</h3><div>We identified adult AH hospitalizations from the Nationwide Readmission Database, further categorized based on PC utilization. Multivariate regression analysis was employed to evaluate the impact of PC on the 90-day readmission rate.</div></div><div><h3>Results</h3><div>Among the 68,062 AH patients, 3,784 (5.56 %) utilized PC services. PC utilization in AH hospitalizations was associated with a significantly lower 90-day readmission rate (adjusted hazard ratio (aHR) 0.45, <em>P</em> = 0.02). 90-day readmission benefits with PC were persistent on analysis of severe (North American Consortium for the Study of End-Stage Liver Disease-Acute-on-Chronic Liver Failure [NACSELD-ACLF] score ≥1) AH population as well (aHR 0.46, <em>P</em> < 0.01). Trend analysis of PC utilization in AH hospitalizations revealed a non-significant change from 5.39 % in 2016 to 5.69 % in 2020 (<em>P</em> = 0.07).</div></div><div><h3>Conclusion</h3><div>This study shows that PC utilization in AH hospitalizations reduces readmissions and healthcare burden. We advocate integrating PC into the comprehensive management of AH. Further research is needed to determine the optimal timing and components of PC interventions for AH patients.</div></div>","PeriodicalId":10424,"journal":{"name":"Clinics and research in hepatology and gastroenterology","volume":"49 4","pages":"Article 102554"},"PeriodicalIF":2.6,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating transcriptome and metabolomics analyses of hepatocellular carcinoma to discover novel biomarkers and drug targets 整合肝细胞癌的转录组学和代谢组学分析，发现新的生物标志物和药物靶点

IF 2.6 4区医学

Clinics and research in hepatology and gastroenterology Pub Date : 2025-02-10 DOI: 10.1016/j.clinre.2025.102546

Ting Yang, Si-Yu Wang

{"title":"Integrating transcriptome and metabolomics analyses of hepatocellular carcinoma to discover novel biomarkers and drug targets","authors":"Ting Yang, Si-Yu Wang","doi":"10.1016/j.clinre.2025.102546","DOIUrl":"10.1016/j.clinre.2025.102546","url":null,"abstract":"<div><h3>Background</h3><div>Hepatocellular carcinoma (HCC) ranks sixth in incidence and third in mortality among all cancers. Chronic infection by hepatitis B and C viruses are the predominant risk factors for HCC, but other factors related to metabolic disorders including diabetes and obesity are also involved.</div></div><div><h3>Methods</h3><div>Ten male HCC patients with chronic HBV infection were included in this study. Primary HCC tissues were obtained from all study participants following liver resection. Normal tissues that were simultaneously collected served as the controls and were defined as tissue at least 5 cm from the tumor edge. Tissues were subjected to untargeted metabolomics and transcriptome analyses.</div></div><div><h3>Results</h3><div>We identified 31 and 41 differentially expressed metabolites (DEMs) in positive and negative ion modes, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that 15 DEMs were enriched in ABC transporters, nine in purine metabolism, eight in central carbon metabolism in cancer, and seven in biosynthesis of amino acids. Regarding the transcriptome analysis, 1,224 significantly up-regulated and 887 down-regulated RNAs were found. KEGG pathway analysis revealed that the most significantly enriched pathways were metabolic pathways. Integrated analysis showed seven pathways that were highly activated in HCC tissues including PI3K/Akt, ABC transporters, caffeine metabolism, carbon metabolism, biosynthesis of amino acids, arginine biosynthesis, alanine, aspartate, and glutamate metabolism.</div></div><div><h3>Conclusion</h3><div>Some DEMs could be biomarkers or therapeutic targets for HCC. Moreover, we found that MAGEB2 was significantly elevated in HCC tissues for the first time, and its association with HCC needs to be explored by functional studies.</div></div>","PeriodicalId":10424,"journal":{"name":"Clinics and research in hepatology and gastroenterology","volume":"49 4","pages":"Article 102546"},"PeriodicalIF":2.6,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the learning curve of intestinal ultrasound in inflammatory bowel disease: A comparative study between novice, regular and expert 了解肠道超声在炎性肠病中的学习曲线：新手、常规和专家的比较研究

IF 2.6 4区医学

Clinics and research in hepatology and gastroenterology Pub Date : 2025-02-09 DOI: 10.1016/j.clinre.2025.102548

Léo Bove , Jérémy Meyer , Michael Collins , Eric Frampas , Arnaud Bourreille , Catherine Le Berre

{"title":"Understanding the learning curve of intestinal ultrasound in inflammatory bowel disease: A comparative study between novice, regular and expert","authors":"Léo Bove , Jérémy Meyer , Michael Collins , Eric Frampas , Arnaud Bourreille , Catherine Le Berre","doi":"10.1016/j.clinre.2025.102548","DOIUrl":"10.1016/j.clinre.2025.102548","url":null,"abstract":"<div><h3>Background</h3><div>Intestinal ultrasound (IUS) has emerged as a valuable tool for monitoring inflammatory bowel disease (IBD), but its reliability depends on the technical skills of the operator.</div></div><div><h3>Aims</h3><div>This study aimed to assess the IUS learning curve of a novice briefly trained to get him familiarized with the ultrasound machine and normal/abnormal IUS images, and a gastroenterologist with formal general ultrasound training and regular IUS experience (> 200 exams), using an expert radiologist as the gold standard.</div></div><div><h3>Methods</h3><div>The ULTRA-IBD study was a single-center, prospective study involving 50 consecutive IBD patients. Three successive IUS examinations were performed on each patient by the 3 operators. Inter-rater agreement on IUS disease activity was calculated using the intraclass correlation coefficient.</div></div><div><h3>Results</h3><div>The experienced gastroenterologist demonstrated consistently good agreement with the radiologist throughout the study, while the novice failed to go beyond poor agreement after 50 examinations, although a faster learning curve was observed in patients without history of IBD surgery.</div></div><div><h3>Conclusion</h3><div>These findings highlight the difficulty of the technical performance of IUS rather than its interpretation. Comprehensive ultrasound training beforehand and between 50 and 200 examinations of experience are necessary before embarking on IUS.</div></div>","PeriodicalId":10424,"journal":{"name":"Clinics and research in hepatology and gastroenterology","volume":"49 3","pages":"Article 102548"},"PeriodicalIF":2.6,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143386980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative wide-field superficial en bloc R0 resection of a unique colorectal high-grade dysplasia: "horizontal full-thickness resection" 创新的宽视场表面整体R0切除一种独特的结直肠高级别不典型增生：“水平全层切除”。

IF 2.6 4区医学

Clinics and research in hepatology and gastroenterology Pub Date : 2025-02-08 DOI: 10.1016/j.clinre.2025.102552

Vincent Zimmer

引用次数: 0

A class of MicroRNAs as diagnostic biomarkers and therapeutic strategies in non-alcoholic fatty liver disease: A review 一类microrna作为非酒精性脂肪性肝病的诊断生物标志物和治疗策略

IF 2.6 4区医学

Clinics and research in hepatology and gastroenterology Pub Date : 2025-02-07 DOI: 10.1016/j.clinre.2025.102547

Faraz Mahdizadeh , Pouria Sobhi , Shokofeh Banaei

{"title":"A class of MicroRNAs as diagnostic biomarkers and therapeutic strategies in non-alcoholic fatty liver disease: A review","authors":"Faraz Mahdizadeh , Pouria Sobhi , Shokofeh Banaei","doi":"10.1016/j.clinre.2025.102547","DOIUrl":"10.1016/j.clinre.2025.102547","url":null,"abstract":"<div><div>MicroRNAs (miRNAs), small and noncoding RNAs that regulate gene expression through hybridization to messenger RNA, play a crucial role in the prevention or progression of non-alcoholic fatty liver disease (NAFLD). There is an urgent demand for the improvement of diagnostic tools and effective pharmacotherapies for the treatment of NAFLD, which can advance to cirrhosis and liver cancer. MiRNAs act as regulatory factors and noninvasive diagnostic agents for NAFLD, enabling the staging of the disorder, prognosis, and identification of pharmaco-therapeutic targets. NAFLD causes alterations in the expression patterns of hepatocyte miRNAs, with some specific miRNAs related to the upgrade from NAFLD to non-alcoholic steatohepatitis (NASH). These miRNAs can activate certain signaling cascade and exacerbate or improve NAFLD, additionally, act as hepatocellular signals or second messengers that transmit information between the liver and other systems. This study provides a comprehensive review of the most important miRNAs and their involvement in the pathophysiology and cellular signaling pathways related to NAFLD.</div></div>","PeriodicalId":10424,"journal":{"name":"Clinics and research in hepatology and gastroenterology","volume":"49 4","pages":"Article 102547"},"PeriodicalIF":2.6,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Management of portal hypertension in patients with hepatocellular carcinoma 肝细胞癌门静脉高压症的处理。

IF 2.6 4区医学

Clinics and research in hepatology and gastroenterology Pub Date : 2025-02-06 DOI: 10.1016/j.clinre.2025.102551

François Faitot

引用次数: 0

The impact of incidental hepatocellular carcinoma in liver transplantation: A growing concern 肝移植中偶发肝细胞癌的影响：日益受到关注

IF 2.6 4区医学

Clinics and research in hepatology and gastroenterology Pub Date : 2025-02-06 DOI: 10.1016/j.clinre.2025.102550

Alfredo Caturano , Enes Erul

{"title":"The impact of incidental hepatocellular carcinoma in liver transplantation: A growing concern","authors":"Alfredo Caturano , Enes Erul","doi":"10.1016/j.clinre.2025.102550","DOIUrl":"10.1016/j.clinre.2025.102550","url":null,"abstract":"<div><div>Incidental hepatocellular carcinoma (iHCC) is a growing concern in liver transplantation, where tumors are unexpectedly discovered during histopathological examination of explanted livers. The prevalence of iHCC varies widely, with reported rates between 1.6 % and 40 %, influenced by differences in diagnostic practices and patient populations. Recent studies, including that by Kerstens et al., highlight the clinical significance of iHCC, revealing its association with alcohol-related liver disease and the limitations of current surveillance strategies. Conventional imaging techniques, such as ultrasound, often fail to detect small tumors, leading to delayed diagnosis. Advanced imaging modalities, including MRI, and emerging biomarkers, such as des-γ-carboxy prothrombin (DCP), are improving detection, but further research is needed to refine diagnostic protocols. The growing recognition of Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) complicates the surveillance of iHCC, particularly as patients with MASLD often present with subtle or atypical clinical profiles. Personalized surveillance, incorporating advanced technologies like liquid biopsy and artificial intelligence, holds promise for improving early detection. Additionally, individualized post-transplant management strategies are crucial, given the lower survival rates observed in iHCC patients. As our understanding of iHCC evolves, a shift toward more tailored approaches in diagnosis, surveillance, and treatment is essential to improve outcomes and reduce the burden of incidental HCC in liver transplantation.</div></div>","PeriodicalId":10424,"journal":{"name":"Clinics and research in hepatology and gastroenterology","volume":"49 3","pages":"Article 102550"},"PeriodicalIF":2.6,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0