Clinical chemistry and laboratory medicine最新文献

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An immuno-DOT diagnostic assay for autoimmune nodopathy. 自身免疫性结节病的免疫 DOT 诊断测定。
IF 6.8 2区 医学
Clinical chemistry and laboratory medicine Pub Date : 2024-06-12 DOI: 10.1515/cclm-2024-0510
Alexandre Jentzer, Guillaume Taieb, Jérémie El Bechir, Thierry Vincent, Jérôme Joël Devaux
{"title":"An immuno-DOT diagnostic assay for autoimmune nodopathy.","authors":"Alexandre Jentzer, Guillaume Taieb, Jérémie El Bechir, Thierry Vincent, Jérôme Joël Devaux","doi":"10.1515/cclm-2024-0510","DOIUrl":"https://doi.org/10.1515/cclm-2024-0510","url":null,"abstract":"<p><strong>Objectives: </strong>Autoimmune nodopathy (AN) is a life-threatening peripheral neuropathy mediated by four autoantibodies targeting axoglial cell adhesion molecules at the nodes of Ranvier: Neurofascin-155 (Nfasc155), PanNeurofascin (PanNfasc), Contactin-1 (CNTN1), and Contactin-associated protein 1 (CASPR1). Antibody detection is a strong biomarker for AN diagnosis and treatment monitoring. The aim of this study was to develop an immuno-dot assay (immuno-DOT) compatible with routine implementation in medical laboratories.</p><p><strong>Methods: </strong>This new approach was compared to standard techniques: indirect immunofluorescence assay, cell-based assay, and ELISA. Sensitivities (Se) and specificities (Sp) were calculated on a cohort composed of 58 patients diagnosed with AN, 50 seronegative patients with chronic inflammatory demyelinating polyradiculoneuropathy, 20 healthy controls, 30 patients with Guillain-Barré syndrome, 20 with monoclonal gammopathy and 20 with Charcot-Marie-Tooth disease. The patients were diagnosed with AN based on compatible electro-clinical arguments and at least two positive standard techniques.</p><p><strong>Results: </strong>Immuno-DOT sensitivities and specificities were Se=91 %, Sp=97 % for anti-Nfasc155; Se=80 %, Sp=94 % for anti-PanNfasc; Se=93 %, Sp=98 % for anti-CNTN1; and Se=87 %, Sp=94 % for anti-CASPR1. Immuno-DOT allowed the diagnosis within 3 h and the accurate follow-up of the immune reactivity and isotype, and dot intensity correlated with antibody titers following treatments. A longitudinal study indicated that immuno-DOT yielded reliable results even after six months of storage at -20 °C.</p><p><strong>Conclusions: </strong>The diagnostic performance of immuno-DOT was satisfactory and compatible with routine implementation in medical laboratories.</p>","PeriodicalId":10390,"journal":{"name":"Clinical chemistry and laboratory medicine","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Methemoglobinemia after sodium nitrite poisoning: what blood gas analysis tells us (and what it might not). 亚硝酸钠中毒后的高铁血红蛋白血症:血气分析能告诉我们什么(以及不能告诉我们什么)。
IF 3.8 2区 医学
Clinical chemistry and laboratory medicine Pub Date : 2024-05-28 Print Date: 2024-10-28 DOI: 10.1515/cclm-2024-0465
Fabrizio Cappellini, Chiara Fania, Loredana Di Simone, Francesco Gaiani, Marco Giani, Marco Casati
{"title":"Methemoglobinemia after sodium nitrite poisoning: what blood gas analysis tells us (and what it might not).","authors":"Fabrizio Cappellini, Chiara Fania, Loredana Di Simone, Francesco Gaiani, Marco Giani, Marco Casati","doi":"10.1515/cclm-2024-0465","DOIUrl":"10.1515/cclm-2024-0465","url":null,"abstract":"","PeriodicalId":10390,"journal":{"name":"Clinical chemistry and laboratory medicine","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Thyroglobulin measurement is the most powerful outcome predictor in differentiated thyroid cancer: a decision tree analysis in a European multicenter series. 甲状腺球蛋白测量是分化型甲状腺癌最有力的预后指标:欧洲多中心系列研究的决策树分析。
IF 3.8 2区 医学
Clinical chemistry and laboratory medicine Pub Date : 2024-05-07 Print Date: 2024-10-28 DOI: 10.1515/cclm-2024-0405
Luca Giovanella, Lisa Milan, Wolfgang Roll, Manuel Weber, Simone Schenke, Michael Kreissl, Alexis Vrachimis, Kim Pabst, Tuncel Murat, Petra Petranović Ovčariček, Alfredo Campenni, Rainer Görges, Luca Ceriani
{"title":"Thyroglobulin measurement is the most powerful outcome predictor in differentiated thyroid cancer: a decision tree analysis in a European multicenter series.","authors":"Luca Giovanella, Lisa Milan, Wolfgang Roll, Manuel Weber, Simone Schenke, Michael Kreissl, Alexis Vrachimis, Kim Pabst, Tuncel Murat, Petra Petranović Ovčariček, Alfredo Campenni, Rainer Görges, Luca Ceriani","doi":"10.1515/cclm-2024-0405","DOIUrl":"10.1515/cclm-2024-0405","url":null,"abstract":"<p><strong>Objectives: </strong>An accurate prognostic assessment is pivotal to adequately inform and individualize follow-up and management of patients with differentiated thyroid cancer (DTC). We aimed to develop a predictive model for recurrent disease in DTC patients treated by surgery and <sup>131</sup>I by adopting a decision tree model.</p><p><strong>Methods: </strong>Age, sex, histology, T stage, N stage, risk classes, remnant estimation, thyroid-stimulating hormone (TSH), thyroglobulin (Tg), administered <sup>131</sup>I activities and post-therapy whole body scintigraphy (PT-WBS) were identified as potential predictors and put into regression algorithm (conditional inference tree, c-tree) to develop a risk stratification model for predicting persistent/recurrent disease over time.</p><p><strong>Results: </strong>The PT-WBS pattern identified a partition of the population into two subgroups (PT-WBS positive or negative for distant metastases). Patients with distant metastases exhibited lower disease-free survival (either structural, DFS-SD, and biochemical, DFS-BD, disease) compared to those without metastases. Meanwhile, the latter were further stratified into three risk subgroups based on their Tg values. Notably, Tg values >63.1 ng/mL predicted a shorter survival time, with increased DFS-SD for Tg values <63.1 and <8.9 ng/mL, respectively. A comparable model was generated for biochemical disease (BD), albeit different DFS were predicted by slightly different Tg cutoff values (41.2 and 8.8 ng/mL) compared to DFS-SD.</p><p><strong>Conclusions: </strong>We developed a simple, accurate and reproducible decision tree model able to provide reliable information on the probability of structurally and/or biochemically persistent/relapsed DTC after a TTA. In turn, the provided information is highly relevant to refine the initial risk stratification, identify patients at higher risk of reduced structural and biochemical DFS, and modulate additional therapies and the relative follow-up.</p>","PeriodicalId":10390,"journal":{"name":"Clinical chemistry and laboratory medicine","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Insight into the status of plasma renin and aldosterone measurement: findings from 526 clinical laboratories in China 血浆肾素和醛固酮测定现状透视:来自中国 526 家临床实验室的研究结果
IF 6.8 2区 医学
Clinical chemistry and laboratory medicine Pub Date : 2024-04-30 DOI: 10.1515/cclm-2024-0373
Weiyan Zhou, Yuhang Deng, Wenjun Ma, Haijian Zhao, Kaijun Wang, Qian Zhang, Wei Gan, Wenxiang Chen, Jun Cai, Chuanbao Zhang
{"title":"Insight into the status of plasma renin and aldosterone measurement: findings from 526 clinical laboratories in China","authors":"Weiyan Zhou, Yuhang Deng, Wenjun Ma, Haijian Zhao, Kaijun Wang, Qian Zhang, Wei Gan, Wenxiang Chen, Jun Cai, Chuanbao Zhang","doi":"10.1515/cclm-2024-0373","DOIUrl":"https://doi.org/10.1515/cclm-2024-0373","url":null,"abstract":"Objectives Accurate measurements of renin and aldosterone levels play an important role in primary aldosteronism screening, which is of great importance in the management and categorization of hypertension. The objective of this study is to investigate the current status of plasma renin and aldosterone measurements in China, which is achieved by analyzing the results of 526 clinical laboratories nationwide for three pooled fresh plasma samples derived from more than 2,000 patients. Methods Renin and aldosterone in three pooled plasma samples were measured four times in 526 laboratories employing various measurement systems. The inter- and intra-laboratory %CV were calculated and compared. To determine the source of the substantial inter-laboratory %CV, laboratories were categorized according to the measurement systems they are using, and both the inter- and intra-measurement-system %CV were calculated and compared. Results Regarding renin, the majority of laboratories use four primary commercial immunoassays. However, for aldosterone, in addition to commercial immunoassays, laboratory-developed liquid chromatography-tandem mass spectrometry (LC-MS) methods are also used by laboratories. The median values of intra-laboratory %CVs, intra-measurement-system %CVs, inter-laboratory %CVs, and inter-measurement systems %CVs varied between 1.6 and 2.6 %, 4.6 and 14.9 %, 8.3 and 25.7 %, and 10.0 and 34.4 % for renin, respectively. For aldosterone, these values ranged from 1.4 to 2.2 %, 2.5–14.7 %, 9.9–31.0 %, and 10.0–35.5 %, respectively. Conclusions The precision within laboratories and measurement systems for plasma renin and aldosterone measurements is satisfactory. However, the comparability between laboratories using different measurement systems remains lacking, indicating the long way to achieve standardization and harmonization for these two analytes.","PeriodicalId":10390,"journal":{"name":"Clinical chemistry and laboratory medicine","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140826997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An improved implementation of metrological traceability concepts is needed to benefit from standardization of laboratory results 需要改进计量可追溯性概念的实施,以便从实验室结果标准化中获益
IF 6.8 2区 医学
Clinical chemistry and laboratory medicine Pub Date : 2024-04-30 DOI: 10.1515/cclm-2024-0428
Mauro Panteghini
{"title":"An improved implementation of metrological traceability concepts is needed to benefit from standardization of laboratory results","authors":"Mauro Panteghini","doi":"10.1515/cclm-2024-0428","DOIUrl":"https://doi.org/10.1515/cclm-2024-0428","url":null,"abstract":"Non-harmonization of laboratory results represents a concrete risk for patient safety. To avoid harms, it is agreed that measurements by <jats:italic>in vitro</jats:italic> diagnostic medical devices (IVD-MD) on clinical samples should be traceable to higher-order references and adjusted to give the same result. However, metrological traceability is not a formal claim and has to be correctly implemented, which in practice does not happen for a non-negligible number of measurands. Stakeholders, such as higher-order reference providers, IVD manufacturers, and External Quality Assessment organizers, have major responsibilities and should improve their contribution by unambiguously and rigorously applying what is described in the International Organization for Standardization 17511:2020 standard and other documents provided by the international scientific bodies, such as Joint Committee on Traceability in Laboratory Medicine and IFCC. For their part, laboratory professionals should take responsibility to abandon non-selective methods and move to IVD-MDs displaying proper selectivity, which is one of the indispensable prerequisites for the correct implementation of metrological traceability. The practicality of metrological traceability concepts is not impossible but relevant education and appropriate training of all involved stakeholders are essential to obtain the expected benefits in terms of standardization.","PeriodicalId":10390,"journal":{"name":"Clinical chemistry and laboratory medicine","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140826998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Macroprolactin in mothers and their babies: what is its origin? 母婴体内的巨泌乳素:其来源是什么?
IF 6.8 2区 医学
Clinical chemistry and laboratory medicine Pub Date : 2024-04-29 DOI: 10.1515/cclm-2024-0235
Norito Nishiyama, Naoki Hattori, Kohozo Aisaka, Masayuki Ishihara, Takanori Saito
{"title":"Macroprolactin in mothers and their babies: what is its origin?","authors":"Norito Nishiyama, Naoki Hattori, Kohozo Aisaka, Masayuki Ishihara, Takanori Saito","doi":"10.1515/cclm-2024-0235","DOIUrl":"https://doi.org/10.1515/cclm-2024-0235","url":null,"abstract":"Objectives Macroprolactinemia is one of the major causes of hyperprolactinemia. The aim of this study was to clarify the origin of macroprolactin (macro-PRL). Methods We examined macro-PRL in the sera of 826 pregnant women and in those of their babies’ umbilical cords at delivery. Macro-PRL was evaluated by precipitation with polyethylene glycol (PEG), gel filtration chromatography (GFC), and absorption with protein G (PG). Results We detected macro-PRL in 16 out of the 826 pregnant women (1.94 %) and in 14 of their babies, which may indicate the possibility of hereditary origin of macro-PRL. However, the macro-PRL ratios of the babies correlated positively with those of their mothers (r=0.72 for GFC, p&lt;0.001 and r=0.77 for PG, p&lt;0.001), suggesting that the immunoglobulin (Ig)G-type anti-PRL autoantibodies might be actively transferred to babies via the placenta and form macro-PRL by binding to their babies’ PRL or PRL-IgG complexes may possibly pass through the placenta. There were two cases in which only mothers had macro-PRL, indicating that the mothers had autoantibodies that did not pass through the placenta, such as IgA, PRL bound to the other proteins or PRL aggregates. No cases were found in which only the babies had macro-PRL and their mothers did not, suggesting that macro-PRL might not arise by non-hereditary congenital causes. Conclusions Macro-PRL in women of reproductive age might be mostly IgG-type anti-PRL autoantibody-bound PRL. The likely origin of macro-PRL in babies is the transplacental transfer of IgG-type anti-PRL autoantibodies or PRL-IgG complexes from the mothers to their babies.","PeriodicalId":10390,"journal":{"name":"Clinical chemistry and laboratory medicine","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140842180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diagnostic performance of a point of care high-sensitivity cardiac troponin I assay and single measurement evaluation to rule out and rule in acute coronary syndrome 高灵敏度心肌肌钙蛋白 I 检测和单次测量评估在排除和诊断急性冠状动脉综合征方面的诊断性能
IF 6.8 2区 医学
Clinical chemistry and laboratory medicine Pub Date : 2024-04-26 DOI: 10.1515/cclm-2024-0225
Vincenzo De Iuliis, Anna Rita Gabriele, Francesca De Santis, Roberta De Rugeriis, Luigi Di Quinzio, Steeve Aloisi, Anna Carla Rosati, Manuela Benvenuto, Donatello Fabiani, Sofia Chiatamone Ranieri
{"title":"Diagnostic performance of a point of care high-sensitivity cardiac troponin I assay and single measurement evaluation to rule out and rule in acute coronary syndrome","authors":"Vincenzo De Iuliis, Anna Rita Gabriele, Francesca De Santis, Roberta De Rugeriis, Luigi Di Quinzio, Steeve Aloisi, Anna Carla Rosati, Manuela Benvenuto, Donatello Fabiani, Sofia Chiatamone Ranieri","doi":"10.1515/cclm-2024-0225","DOIUrl":"https://doi.org/10.1515/cclm-2024-0225","url":null,"abstract":"Objectives About 10 million individuals in USA presented annually in the emergency department (ED) with chest pain or with signs and symptoms of acute coronary syndrome (ACS). The advent of point of care (POC) devices, able to measure high sensitivity troponin, are a very interesting tool in the ED setting for its rapid turnaround time (&lt;10 min). Methods The present study evaluates the diagnostic performance of the Atellica VTLi (Siemens) in real life setting using the clinical data derived from integrated diagnoses of emergency room staff and cardiologist and in comparison with standard laboratory hs-cTnT assay (Cobas 8000, Elecsys, Roche). 966 patients admitted to the emergency department of “G. Mazzini Hospital” in Teramo, Italy, from July 27, 2022, through June 09, 2023, were enrolled. Results The diagnostic performance of POC hs-cTnI was evaluated. An appropriate POC hs-cTnI threshold values &lt;4 ng/L supplied a sensitivity and an NPV of 100 % (95 % CI: 99.5–100) in order to achieve rapid rule out for MI through a single measurement at patient presentation in the ED. Furthermore, a derivation POC hs-cTnI concentration &gt;54 ng/L provided a specificity of 97.2 % (95 % CI: 95.9–98.1) and a PPV of 43.5 % (95 % CI: 40.3–46.7) for ruling in MI. Conclusions This platform showed comparable diagnostic performance for myocardial infarction to the central laboratory. Our data suggest the possible use of the Atellica VTLi hs-cTnI POC assay either in emergency department of urban medical centre, either in rural hospital for triage and patient management.","PeriodicalId":10390,"journal":{"name":"Clinical chemistry and laboratory medicine","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140797983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quality control in the Netherlands; todays practices and starting points for guidance and future research. 荷兰的质量控制;当今的做法以及指导和未来研究的出发点。
IF 6.8 2区 医学
Clinical chemistry and laboratory medicine Pub Date : 2024-04-26 DOI: 10.1515/cclm-2024-0316
H. V. van Rossum, Marith van Schrojenstein Lantman, Michel Severens, H. J. Vermeer, W. V. D. Verboeket-van de Venne, Wytze Oosterhuis, Niels de Jonge
{"title":"Quality control in the Netherlands; todays practices and starting points for guidance and future research.","authors":"H. V. van Rossum, Marith van Schrojenstein Lantman, Michel Severens, H. J. Vermeer, W. V. D. Verboeket-van de Venne, Wytze Oosterhuis, Niels de Jonge","doi":"10.1515/cclm-2024-0316","DOIUrl":"https://doi.org/10.1515/cclm-2024-0316","url":null,"abstract":"OBJECTIVES\u0000Adequate analytical quality of reported results is primarily ensured by performing internal quality control (iQC). Currently, several different iQC practices are in use. As a prelude to the revision of a Dutch guidance document on analytical QC, a questionnaire was sent out to gain insights in the applied practices and the need for guidance.\u0000\u0000\u0000METHODS\u0000A questionnaire, containing 20 multiple-choice questions with possibilities for explanation and comment on iQC practices and aspects was distributed to all clinical chemistry laboratories within the Netherlands. Results were reported descriptively.\u0000\u0000\u0000RESULTS\u0000Responses were received from 27 clinical laboratories (response 43 %). In 30 % the iQC was based on the analytical characteristics only, while 30 % used a 6-Sigma method, 19 % risk-based beyond 6-Sigma and 22 % used an alternative approach. 89 % of laboratories used a virtual analyzer model for iQC setup within one or more laboratory sites. Practices for determining standard deviation (SD) values included determining SD for each new iQC material (35 %), using historical SD values for new materials (35 %), and incorporating clinical tolerances into the SD value (31 %). Furthermore, 44 % of laboratories used patient moving averages for one or more tests. Daily iQC management was based on either \"traffic lights\" indicating in or out of control status, and review of all QC charts, often using multiple software systems.\u0000\u0000\u0000CONCLUSIONS\u0000A large heterogeneity of iQC practices in clinical laboratories was observed in the Netherlands. Several starting points for further research and/or guidance were identified, particularly in relation to the determination of SD values, the virtual analyzer model and methods to ensure analyzer equivalence.","PeriodicalId":10390,"journal":{"name":"Clinical chemistry and laboratory medicine","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140652611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Novel thiopurine S-methyltransferase (TPMT) variant identified in Malay individuals. 在马来人中发现新的硫嘌呤 S-甲基转移酶(TPMT)变体。
IF 6.8 2区 医学
Clinical chemistry and laboratory medicine Pub Date : 2024-04-26 DOI: 10.1515/cclm-2024-0494
Chun Kiat Lee, Pei-Tee Huan, Chean Nee Chai, Li Jie Ng, Evelyn Siew-Chuan Koay, Ogestelli Fabia Lee, Malcolm Tan, T. P. Loh
{"title":"Novel thiopurine S-methyltransferase (TPMT) variant identified in Malay individuals.","authors":"Chun Kiat Lee, Pei-Tee Huan, Chean Nee Chai, Li Jie Ng, Evelyn Siew-Chuan Koay, Ogestelli Fabia Lee, Malcolm Tan, T. P. Loh","doi":"10.1515/cclm-2024-0494","DOIUrl":"https://doi.org/10.1515/cclm-2024-0494","url":null,"abstract":"","PeriodicalId":10390,"journal":{"name":"Clinical chemistry and laboratory medicine","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140652975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Skin in the game: a review of single-cell and spatial transcriptomics in dermatological research. 游戏中的皮肤:单细胞和空间转录组学在皮肤病研究中的应用综述。
IF 6.8 2区 医学
Clinical chemistry and laboratory medicine Pub Date : 2024-04-25 DOI: 10.1515/cclm-2023-1245
Samuel Schepps, Jonathan Xu, Henry Yang, Jenna Mandel, Jaanvi Mehta, Julianna Tolotta, Nicole Baker, Volkan Tekmen, Neda Nikbakht, Paolo Fortina, Ignacia Fuentes, Bonnie LaFleur, Raymond J Cho, Andrew P South
{"title":"Skin in the game: a review of single-cell and spatial transcriptomics in dermatological research.","authors":"Samuel Schepps, Jonathan Xu, Henry Yang, Jenna Mandel, Jaanvi Mehta, Julianna Tolotta, Nicole Baker, Volkan Tekmen, Neda Nikbakht, Paolo Fortina, Ignacia Fuentes, Bonnie LaFleur, Raymond J Cho, Andrew P South","doi":"10.1515/cclm-2023-1245","DOIUrl":"https://doi.org/10.1515/cclm-2023-1245","url":null,"abstract":"Single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) are two emerging research technologies that uniquely characterize gene expression microenvironments on a cellular or subcellular level. The skin, a clinically accessible tissue composed of diverse, essential cell populations, serves as an ideal target for these high-resolution investigative approaches. Using these tools, researchers are assembling a compendium of data and discoveries in healthy skin as well as a range of dermatologic pathophysiologies, including atopic dermatitis, psoriasis, and cutaneous malignancies. The ongoing advancement of single-cell approaches, coupled with anticipated decreases in cost with increased adoption, will reshape dermatologic research, profoundly influencing disease characterization, prognosis, and ultimately clinical practice.","PeriodicalId":10390,"journal":{"name":"Clinical chemistry and laboratory medicine","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140658456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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