Clinical and Experimental Nephrology最新文献_第3页

Glomerular filtration of creatinine: expanding its clinical utility as a skeletal muscle mass index in non-dialysis CKD patients. 肾小球滤过肌酐：扩大其作为非透析慢性肾脏病患者骨骼肌质量指数的临床用途。

IF 2.2 4区医学

Clinical and Experimental Nephrology Pub Date : 2025-03-29 DOI: 10.1007/s10157-025-02670-3

Shunsuke Yamada, Toshiaki Nakano

引用次数: 0

Status of initial treatment for rapidly progressive glomerulonephritis in Japan: analysis of a personal clinical records database. 日本快速进展性肾小球肾炎的初始治疗状况：个人临床记录数据库分析。

IF 2.2 4区医学

Clinical and Experimental Nephrology Pub Date : 2025-03-27 DOI: 10.1007/s10157-025-02657-0

Joichi Usui, Tomonori Kimura, Kunihiro Yamagata, Kentaro Nakajima, Shuzo Kaneko, Ken-Ei Sada, Naotake Tsuboi, Hirokazu Okada, Keiju Hiromura, Yoshitaka Isaka, Ichiei Narita

{"title":"Status of initial treatment for rapidly progressive glomerulonephritis in Japan: analysis of a personal clinical records database.","authors":"Joichi Usui, Tomonori Kimura, Kunihiro Yamagata, Kentaro Nakajima, Shuzo Kaneko, Ken-Ei Sada, Naotake Tsuboi, Hirokazu Okada, Keiju Hiromura, Yoshitaka Isaka, Ichiei Narita","doi":"10.1007/s10157-025-02657-0","DOIUrl":"https://doi.org/10.1007/s10157-025-02657-0","url":null,"abstract":"Background: As a joint project with the Ministry of Health, Labour and Welfare (MHLW), the Research Group on Intractable Renal Diseases is examining the feasibility of utilizing its personal clinical records database. We examine the validity of the initial-treatment data from the personal clinical records of patients with new-onset rapidly progressive glomerulonephritis (RPGN).Methods: Personal clinical records for patients with either RPGN or anti-glomerular basement membrane (GBM) antibody nephritis were used. The data from 454 newly enrolled RPGN patients were compiled for analysis in 2 cohort studies (CS1 for all case analysis and CS2 for selective analysis of new-onset cases).Results: In CS1, the serotypes of the 362 registered RPGN cases included 200 myeloperoxidase (MPO)-ANCA-positive, 98 anti-GBM-positive, and 9 proteinase-3 (PR3)-ANCA-positive cases, etc. CS2 included 96 of the MPO-ANCA-positive RPGN and 55 of the anti-GBM antibody-positive RPGN cases. For the initial treatment of MPO-ANCA-positive RPGN, the rates of glucocorticoid (GC) and GC pulse treatment were similar between the personal clinical records database and the nationwide questionnaire survey, but the rates of intravenous cyclophosphamide (CY) or rituximab were statistically significant lower in the personal clinical records database. For the initial treatment of anti-GBM antibody-positive RPGN, the rate of plasma exchange was similar between the two databases, but the rates of GC and per os CY tended to be lower in the personal clinical records database, although not statistically significant.Conclusion: Clear differences in initial treatment for new-onset RPGN patients were found between a personal clinical records database and another reported database.","PeriodicalId":10349,"journal":{"name":"Clinical and Experimental Nephrology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human primary proximal tubular epithelial cells and sepsis: a scoping review. 人类原发性近端肾小管上皮细胞与败血症：范围综述。

IF 2.2 4区医学

Clinical and Experimental Nephrology Pub Date : 2025-03-26 DOI: 10.1007/s10157-025-02656-1

Kjellbjørn Jakobsen, Ludvik K Ellingsen, Eirik Reierth, Stephen J Hodges, Lars M Ytrebø

{"title":"Human primary proximal tubular epithelial cells and sepsis: a scoping review.","authors":"Kjellbjørn Jakobsen, Ludvik K Ellingsen, Eirik Reierth, Stephen J Hodges, Lars M Ytrebø","doi":"10.1007/s10157-025-02656-1","DOIUrl":"https://doi.org/10.1007/s10157-025-02656-1","url":null,"abstract":"Background: Sepsis impairs proximal renal tubular epithelial cell (PTEC) function, and this impairment contributes to the pathophysiology of sepsis-associated acute kidney injury (SA-AKI). By closely replicating in vivo conditions, primary human PTEC offer superior biological relevance for studying SA-AKI. The purpose of this scoping review was to identify and investigate experiments, where human primary PTEC have been used to study sepsis-related factors.Methods: A comprehensive literature search strategy was developed, and our reported items adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for scoping reviews (PRISMA-ScR) checklist. Peer-reviewed articles published in English or Scandinavian languages between 1946 and 2024 were included.Results: The literature search provided 292 results. Twelve studies were included, out of which only two were published after 2010. Eight studies used human primary PTEC isolated from healthy tissue during tumor nephrectomy, while four studies used primary PTEC purchased from commercially available providers. Experimental methods were heterogenous. The included studies applied E. coli porine, E. coli, Staphylococcal enterotoxin B, cytokines and lipopolysaccharide with differing dosages, exposure lengths, and combinations.Conclusions: Although human primary PTEC more closely resembles the in vivo environment in human kidneys, their use in sepsis and SA-AKI research remains remarkably limited, leading to substantial research gaps in the field. In addition, there is significant heterogeneity in the methodologies employed across existing studies. Standardizing and expanding the use of primary PTECs in future in vitro research could be pivotal for unraveling novel and relevant insights into the pathophysiology of SA-AKI.","PeriodicalId":10349,"journal":{"name":"Clinical and Experimental Nephrology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wnt-5a ameliorates sepsis-induced downregulation of renal AQP2 via the calcineurin signaling pathway. Wnt-5a通过钙调磷酸酶信号通路改善脓毒症诱导的肾AQP2下调。

IF 2.2 4区医学

Clinical and Experimental Nephrology Pub Date : 2025-03-26 DOI: 10.1007/s10157-025-02664-1

Yuanlong Wang, Shuai Xia, Wei Teng, Yufan Du, Hongli Xiao, Guoxing Wang

{"title":"Wnt-5a ameliorates sepsis-induced downregulation of renal AQP2 via the calcineurin signaling pathway.","authors":"Yuanlong Wang, Shuai Xia, Wei Teng, Yufan Du, Hongli Xiao, Guoxing Wang","doi":"10.1007/s10157-025-02664-1","DOIUrl":"https://doi.org/10.1007/s10157-025-02664-1","url":null,"abstract":"Background: Aquaporin 2 (AQP2), a vasopressin-sensitive water channel in the renal collecting ducts, maintains body water homeostasis. Sepsis reduces AQP2 and vasopressin receptor 2 (V2R) levels, contributing to acute kidney injury. In a heritable nephrogenic diabetes insipidus mouse model with V2R mutation, Wnt-5a, a ligand for frizzled receptors (Fzds), enhances AQP2 expression and translocation through calcium/calmodulin/calcineurin signaling. However, the mechanism by which Wnt-5a reduces sepsis-induced AQP2 inhibition remains unclear. We assessed this mechanism and whether Wnt-5a alleviates sepsis-induced downregulation of renal AQP2.Methods: Our study used in vitro and in vivo approaches. To study AQP2 trafficking, lipopolysaccharide (LPS) was applied to mouse inner medullary collecting duct 3 (mIMCD3) cells in vitro; subsequently, phosphorylated AQP2 and apical AQP2 expression as well as calcineurin activity and its upstream regulators (endogenous Wnt-5a, Fzds, and intracellular calcium intensity) were examined. In vivo, cecal ligation and puncture (CLP) mice were used to assess AQP2 levels and urine osmolality as indicators of urinary concentration.Results: In mIMCD3 cells, LPS application was associated with reduced V2R expression, a vasopressin-irreversible reduction in AQP2, and increased Fzds turnover and Wnt-5a-stimulated intracellular calcium, enhancing calcineurin signaling. Subsequent application of Wnt-5a to LPS-exposed mIMCD3 cells prevented AQP2 reduction. Moreover, it increased phosphorylated AQP2 at residue Ser269 (Ser269-pAQP2) and decreased at Ser261-pAQP2 without affecting Ser256-pAQP2. These effects were reversed by the calcineurin inhibitor cyclosporin A. In CLP mice, Wnt-5a injection was associated with increased renal AQP2 and urine osmolality.Conclusion: Wnt-5a attenuates sepsis-induced AQP2 downregulation through the calcineurin signaling pathway.","PeriodicalId":10349,"journal":{"name":"Clinical and Experimental Nephrology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical predictors associated with prolonged pneumoperitoneum time in laparoscopic living donor nephrectomy. 腹腔镜活体供体肾切除术中与腹腔积气时间延长相关的临床预测因素。

IF 2.2 4区医学

Clinical and Experimental Nephrology Pub Date : 2025-03-26 DOI: 10.1007/s10157-025-02663-2

Hiroki Kato, Keita Nakane, Ayaka Okamoto, Teppei Nishiwaki, Kojiro Niwa, Masayuki Tomioka, Tomoki Taniguchi, Makoto Kawase, Kota Kawase, Koji Iinuma, Yuki Tobisawa, Takuya Koie

{"title":"Clinical predictors associated with prolonged pneumoperitoneum time in laparoscopic living donor nephrectomy.","authors":"Hiroki Kato, Keita Nakane, Ayaka Okamoto, Teppei Nishiwaki, Kojiro Niwa, Masayuki Tomioka, Tomoki Taniguchi, Makoto Kawase, Kota Kawase, Koji Iinuma, Yuki Tobisawa, Takuya Koie","doi":"10.1007/s10157-025-02663-2","DOIUrl":"https://doi.org/10.1007/s10157-025-02663-2","url":null,"abstract":"Background: Kidney transplantation (KT) is a useful treatment option for patients with end-stage chronic kidney disease to avoid dialysis and achieve a good quality of life. In Japan, approximately 90% of kidneys for KT are obtained from living kidney donors. Laparoscopic renal nephrectomy (LDN) is the most commonly performed KT procedure in Japan. We aimed to determine the clinical variables that influence the prolongation of pneumoperitoneum time (PT) in LDN.Method: This retrospective study was carried out on 218 consecutive patients who underwent LDN at Gifu University Hospital. T The enrolled patients were divided into two groups according to the third quartile of PT in the LDN, with those in the lower third quartile (Q3) as Group 1 and those in the upper Q3 as Group 2. The primary endpoint was identification of predictive factors for prolonged PT.Result: In total, 178 patients were included in the analysis. For all patients, the median PT, estimated blood loss, and warm ischemic time were 170 min, 20 mL, and 4 min, respectively. Significantly longer PT was observed in Group 2 than in Group 1. Multiple regression analysis results showed that male donors, body mass index ≥ 25 kg/m2, and donors with more than two renal arteries were independent predictive factors for PT prolongation.Conclusion: Male sex, BMI ≥ 25 kg/m2, and two or more renal arteries are predictive factors for prolonged PT for donors in LDN.","PeriodicalId":10349,"journal":{"name":"Clinical and Experimental Nephrology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of magnetic field therapy (AT-04) on pain reduction during arteriovenous fistula puncture in hemodialysis. 磁场治疗（AT-04）对减轻血液透析动静脉瘘穿刺疼痛的影响。

IF 2.2 4区医学

Clinical and Experimental Nephrology Pub Date : 2025-03-26 DOI: 10.1007/s10157-025-02666-z

Yukiko Tsuchiya, Taketo Uchiyama, Kazuhiko Kato, Akio Nakashima, Goro Tokudome, Takashi Yokoo

{"title":"Effects of magnetic field therapy (AT-04) on pain reduction during arteriovenous fistula puncture in hemodialysis.","authors":"Yukiko Tsuchiya, Taketo Uchiyama, Kazuhiko Kato, Akio Nakashima, Goro Tokudome, Takashi Yokoo","doi":"10.1007/s10157-025-02666-z","DOIUrl":"https://doi.org/10.1007/s10157-025-02666-z","url":null,"abstract":"Background: Pain from arteriovenous fistula puncture significantly affects the quality of life of patients undergoing hemodialysis. AT-04 was designed to alleviate this pain using magnetic stimulation, which is anticipated to be beneficial for managing pain across different conditions. However, limited research exists on the effectiveness of AT-04 in diminishing the pain related to arteriovenous fistula puncture in hemodialysis patients.Methods: Between July and August 2024, we enrolled 14 outpatient maintenance hemodialysis patients at Tokyu Hospital who had given informed consent. AT-04 was administered before arteriovenous fistula puncture. We assessed the pain intensity of the puncture using the Visual Analog Scale (VAS) before the application of AT-04, as well as at one and four weeks afterward. We observed changes in puncture pain levels before and after the AT-04 application.Results: The participants had an average age of 80 years (IQR, 74-85) and a mean dialysis duration of 5.8 years (IQR, 1.0-8.9). Lidocaine tape was utilized by 71.4% of participants, painkillers by 7.1%, and 28.6% used sleeping pills, with none taking antidepressants. The median VAS score before starting AT-04 was 40.5 (IQR, 23.5-55.5). One week after starting AT-04, the median VAS score dropped to 27.0 (IQR, 4.5-32.8), and four weeks later, it further decreased to 16.5 (IQR, 5.0-38.8). These decreases were statistically significant compared to the pre-treatment VAS score (one week after p = 0.0057, four weeks after p = 0.0076).Conclusion: The AT-04 device, which uses alternating magnetic field therapy, significantly alleviated pain from arteriovenous fistula puncture in hemodialysis patients.","PeriodicalId":10349,"journal":{"name":"Clinical and Experimental Nephrology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal changes of the life and renal prognoses of patients with rapidly progressive glomerulonephritis in Japan, 1989-2019. 1989-2019年日本快速进展性肾小球肾炎患者寿命和肾脏预后的时间变化

IF 2.2 4区医学

Clinical and Experimental Nephrology Pub Date : 2025-03-25 DOI: 10.1007/s10157-025-02643-6

Kentaro Nakajima, Shuzo Kaneko, Joichi Usui, Naotake Tsuboi, Hitoshi Sugiyama, Shoichi Maruyama, Yoshitaka Isaka, Ichiei Narita, Kunihiro Yamagata

{"title":"Temporal changes of the life and renal prognoses of patients with rapidly progressive glomerulonephritis in Japan, 1989-2019.","authors":"Kentaro Nakajima, Shuzo Kaneko, Joichi Usui, Naotake Tsuboi, Hitoshi Sugiyama, Shoichi Maruyama, Yoshitaka Isaka, Ichiei Narita, Kunihiro Yamagata","doi":"10.1007/s10157-025-02643-6","DOIUrl":"https://doi.org/10.1007/s10157-025-02643-6","url":null,"abstract":"Background: This study is a continuation of the Japan Rapidly Progressive GlomeruloNephritis (RPGN) Working Group's chronological nationwide survey.Methods: We analyzed 1,660 RPGN cases from 2016-2019 and compared them to 4,179 cases from five earlier periods (1989-1998, 1999-2001, 2002-2008, 2009-2011, 2012-2015). Data on causative diseases, clinical severity, 24-month life and renal survival, and treatment details were collected and compared.Results: The most recent cohort showed an older median age at onset (median age 74 years), with improved serum creatinine levels (median 2.5 mg/dL). Cumulative survival at 24 months remained stable (periods 1989-1998, 1999-2001, 2002-2008, 2009-2011, 2012-2015, 2016-2019 were each 72.0%, 72.9%, 77.7%, 83.0%, 84.9%, 83.5%, p < 0.01), while renal survival showed a favorable trend in the most recent periods (there were each 68.7%, 75.4%, 76.7%, 73.4%, 78.2%, 78.4%, p < 0.01). Anti-neutrophil cytoplasmic antibody-associated vasculitis (AAV)-RPGN had similar outcomes to the overall cohort. Increased rituximab use was observed, with no significant differences in life and renal prognosis between rituximab (RIX) and cyclophosphamide (CY). In severe renal impairment (Cre ≥ 6), renal prognosis was better in the CY or RIX use group than in the non-use group (p = 0.035, 0.025). Anti-glomerular basement membrane disease had a poorer renal prognosis compared to other causes.Conclusions: Despite an increasingly older age of onset, both life and renal prognoses for new-onset AAV-RPGN from 2016 to 2019 remain comparable to the best in previous surveys, due to the impact of constant improvements in early diagnosis and changes in treatment.","PeriodicalId":10349,"journal":{"name":"Clinical and Experimental Nephrology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autophagic stagnation: a key mechanism in kidney disease progression linked to aging and obesity. 自噬停滞：与衰老和肥胖相关的肾脏疾病进展的关键机制

IF 2.2 4区医学

Clinical and Experimental Nephrology Pub Date : 2025-03-25 DOI: 10.1007/s10157-025-02653-4

Takeshi Yamamoto

{"title":"Autophagic stagnation: a key mechanism in kidney disease progression linked to aging and obesity.","authors":"Takeshi Yamamoto","doi":"10.1007/s10157-025-02653-4","DOIUrl":"https://doi.org/10.1007/s10157-025-02653-4","url":null,"abstract":"Autophagy, a critical intracellular degradation and recycling pathway mediated by lysosomes, is essential for maintaining cellular homeostasis through the quality control of proteins and organelles. Our research focused on the role of proximal tubular autophagy in the pathophysiology of aging, obesity, and diabetes. Using a novel method to monitor autophagic flux in kidney tissue, we revealed that age-associated high basal autophagy supports mitochondrial quality control and delays kidney aging. However, an impaired ability to upregulate autophagy under additional stress accelerates kidney aging. In obesity induced by a high-fat diet, lysosomal dysfunction disrupts autophagy, leading to renal lipotoxicity. Although autophagy is initially activated to repair organelle membranes and maintain proximal tubular cell integrity, this demand overwhelms lysosomes, resulting in \"autophagic stagnation\" characterized by phospholipid accumulation. Similar lysosomal phospholipid accumulation was observed in renal biopsies from elderly and obese patients. We identified TFEB-mediated lysosomal exocytosis as a mechanism to alleviate lipotoxicity by expelling accumulated phospholipids. Therapeutically, interventions such as the SGLT2 inhibitor empagliflozin and eicosapentaenoic acid restore lysosomal function and autophagic activity. Based on these findings, we propose a novel disease concept, \"Obesity-Related Proximal Tubulopathy.\" This study underscores autophagic stagnation as a key driver of kidney disease progression in aging and obesity, offering insights into the pathophysiology of kidney diseases and providing a foundation for targeted therapeutic strategies.","PeriodicalId":10349,"journal":{"name":"Clinical and Experimental Nephrology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platelet-rich plasma improves cyclophosphamide-induced interstitial cystitis in rat models through the toll-like receptor 4/nuclear factor-kappa B signalling pathway. 富血小板血浆通过toll样受体4/核因子κ B信号通路改善环磷酰胺诱导的大鼠间质性膀胱炎模型。

IF 2.2 4区医学

Clinical and Experimental Nephrology Pub Date : 2025-03-25 DOI: 10.1007/s10157-025-02660-5

Yufan Wu, Lei Chen, Minzhe Xu, Linya Yao, Shiyao Yang, Xiaojie Ang, Weiguo Chen

{"title":"Platelet-rich plasma improves cyclophosphamide-induced interstitial cystitis in rat models through the toll-like receptor 4/nuclear factor-kappa B signalling pathway.","authors":"Yufan Wu, Lei Chen, Minzhe Xu, Linya Yao, Shiyao Yang, Xiaojie Ang, Weiguo Chen","doi":"10.1007/s10157-025-02660-5","DOIUrl":"https://doi.org/10.1007/s10157-025-02660-5","url":null,"abstract":"Objective: To investigate the therapeutic effect of platelet-rich plasma (PRP) on a cyclophosphamide (CYP)-induced interstitial cystitis (IC) rat model.Methods: A CYP-induced IC rat model (75 mg/kg every 3 days, with a total of five injections) was used to evaluate the therapeutic effects of PRP. Here, PRP was administered via bladder irrigation (every 2 days, with a total of three irrigations), and bladder tissue was analysed for inflammation and histological changes. The toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB) signalling pathway was assessed using real-time quantitative polymerase chain reaction and ribonucleic acid sequencing. In addition, lipopolysaccharide (LPS)-induced SV-HUC-1 cells (10 μg/LPS and 2.5 mM adenosine triphosphate) were employed to investigate the inflammatory response and the effects of PRP on the TLR4/NF-κB signalling pathway.Results: The PRP treatment significantly improved the bladder tissue condition in the CYP-induced IC rat model, as evidenced by reduced inflammation and histological damage. The damage and shedding of the superficial epithelium of the bladder mucosa were notably decreased following PRP bladder instillation. Importantly, the expression of ZO-1, a key marker of epithelial integrity, was upregulated in PRP-treated rats, indicating enhanced bladder epithelial function. High-throughput analysis revealed that PRP alleviated bladder mucosal injury in the IC rat model through the TLR4/NF-κB signalling pathway. In LPS-induced SV-HUC-1 cells, PRP treatment also increased ZO-1 expression, decreased CDH1 expression and regulated the TLR4/NF-κB signalling pathway.Conclusion: Platelet-rich plasma treatment may improve the expression of ZO-1 and CDH1 in urinary epithelium in vitro by mediating the TLR4/NF-κB pathway, which is effective in the treatment of IC.","PeriodicalId":10349,"journal":{"name":"Clinical and Experimental Nephrology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New interpretation of diabetic nephropathy or diabetic kidney disease from kidney biopsy: review article. 肾活检对糖尿病肾病或糖尿病肾病的新解释：综述文章。

IF 2.2 4区医学

Clinical and Experimental Nephrology Pub Date : 2025-03-24 DOI: 10.1007/s10157-025-02661-4

Yoshifumi Ubara, Naoki Sawa, Masayuki Yamanouchi, Kei Kono, Kenichi Ohashi

{"title":"New interpretation of diabetic nephropathy or diabetic kidney disease from kidney biopsy: review article.","authors":"Yoshifumi Ubara, Naoki Sawa, Masayuki Yamanouchi, Kei Kono, Kenichi Ohashi","doi":"10.1007/s10157-025-02661-4","DOIUrl":"https://doi.org/10.1007/s10157-025-02661-4","url":null,"abstract":"Many nephrologists considered that renal involvement in diabetes patients was seen as nodular glomerulosclerosis (i.e., Kimmelstiel-Wilson lesions). However, they diagnosed diabetic nephropathy or diabetic kidney disease (DKD) from clinical information on diabetes history, and reports of evaluation by kidney biopsy were scarce. Since the publication of the Tervaert classification of diabetic nephropathy in 2010, reports of kidney biopsy in these patients have increased. Analysis of biopsy specimens revealed not only the Tervaert classification (class I to IV) based on glomerular lesions, but also various other pathologies. Besides nodular lesions, findings included paratubular basement membrane insudative lesions (PTBMILs), polar vasculosis, and nephropathy associated with novel drugs (sodium-glucose transport protein 2 inhibitors and dipeptidyl peptidase-4 inhibitors). PTBMILs are unique to diabetic nephropathy. In patients with continuous hyperglycemia and excessive salt intake, elevated blood osmolality (calculated by serum Na × 2 + serum glucose/18 + serum urea nitrogen/2.8) leads to thirst and excessive drinking, which results in fluid overload and generalized edema. The increase in circulating blood volume is thought to induce PTBMILs because of the influx of serum components into the endothelium of glomerular vessels. This case series review presents diverse kidney biopsy findings in patients with diabetic nephropathy or diabetic kidney disease as well as the pathogenesis.","PeriodicalId":10349,"journal":{"name":"Clinical and Experimental Nephrology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0